Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P24583 (KPC1_YEAST)

Last modified June 16, 2009. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protein kinase C-like 1
      Short name=PKC 1
    EC=2.7.11.13
Gene names
Name: PKC1
Synonyms: HPO2, STT1
Ordered Locus Names: YBL105C
ORF Names: YBL0807
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1151 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Required for cell growth and for the G2->M transition of the cell division cycle. Mediates a protein kinase cascade; it activates BCK1 which itself activates MKK1/MKK2.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Contains 2 REM (Hr1) repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ENO1P009241EBI-9860,EBI-6468

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11511151Protein kinase C-like 1
PRO_0000055738

Regions

Repeat5 – 7773REM 1
Repeat118 – 19376REM 2
Domain196 – 28893C2
Domain824 – 1083260Protein kinase
Domain1084 – 115168AGC-kinase C-terminal
Zinc finger414 – 46148Phorbol-ester/DAG-type 1
Zinc finger481 – 53151Phorbol-ester/DAG-type 2
Nucleotide binding830 – 8389ATP By similarity

Sites

Active site9491Proton acceptor By similarity
Binding site8531ATP By similarity

Amino acid modifications

Modified residue2261Phosphoserine Ref.5
Modified residue5771Phosphoserine Ref.5 Ref.4
Modified residue7511Phosphothreonine Ref.5
Modified residue7611Phosphoserine Ref.5 Ref.4
Modified residue9811Phosphothreonine Ref.5
Modified residue9831Phosphothreonine Ref.5
Modified residue11251Phosphothreonine Ref.5

Natural variations

Natural variant9581T → I in cly5; temperature-sensitive mutation that cause cell lysis at high temperature.
Natural variant10231P → S in cly7; temperature-sensitive mutation that cause cell lysis at high temperature.

Experimental info

Sequence conflict811F → C in AAA34878. Ref.1
Sequence conflict2441T → S in AAA34878. Ref.1
Sequence conflict6061G → E in AAA34878. Ref.1
Sequence conflict6231S → P in AAA34878. Ref.1
Sequence conflict7891P → A in AAA34878. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24583-1 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 3300E8D523C8587C

FASTA1,151131,519
        10         20         30         40         50         60 
MSFSQLEQNI KKKIAVEENI IRGASALKKK TSNVMVIQKC NTNIREARQN LEYLEDSLKK 

        70         80         90        100        110        120 
LRLKTAQQSQ GENGSEDNER FNSKEYGFLS TKSPNEHIFS RLDLVKYDCP SLAQRIQYML 

       130        140        150        160        170        180 
QQLEFKLQVE KQYQEANTKL TKLYQIDGDQ RSSSAAEGGA MESKYRIQML NKALKKYQAI 

       190        200        210        220        230        240 
NVDFDQFKHQ PNDIMDNQQP KFRRKQLTGV LTIGITAARD VDHIQSPMFA RKPESYVTIK 

       250        260        270        280        290        300 
IDDTIKARTK PSRNDRWSED FQIPVEKGNE IEITVYDKVN DSLIPVAIMW LLLSDIAEEI 

       310        320        330        340        350        360 
RKKKAGQTNE QQGWVNASNI NGGSSLASEE GSTLTSTYSN SAIQSTSAKN VQGENTSTSQ 

       370        380        390        400        410        420 
ISTNSWFVLE PSGQILLTLG FHKSSQIERK QLMGGLHRHG AIINRKEEIF EQHGHHFVQK 

       430        440        450        460        470        480 
SFYNIMCCAY CGDFLRYTGF QCQDCKFLCH KKCYTNVVTK CIAKTSTDTD PDEAKLNHRI 

       490        500        510        520        530        540 
PHRFLPTSNR GTKWCCHCGY ILPWGRHKVR KCSECGIMCH AQCAHLVPDF CGMSMEMANK 

       550        560        570        580        590        600 
ILKTIQDTKR NQEKKKRTVP SAQLGSSIGT ANGSDLSPSK LAERANAPLP PQPRKHDKTP 

       610        620        630        640        650        660 
SPQKVGRDSP TKQHDPIIDK KISLQTHGRE KLNKFIDENE AYLNFTEGAQ QTAEFSSPEK 

       670        680        690        700        710        720 
TLDPTSNRRS LGLTDLSIEH SQTWESKDDL MRDELELWKA QREEMELEIK QDSGEIQEDL 

       730        740        750        760        770        780 
EVDHIDLETK QKLDWENKND FREADLTIDS THTNPFRDMN SETFQIEQDH ASKEVLQETV 

       790        800        810        820        830        840 
SLAPTSTHPS RTTDQQSPQK SQTSTSAKHK KRAAKRRKVS LDNFVLLKVL GKGNFGKVIL 

       850        860        870        880        890        900 
SKSKNTDRLC AIKVLKKDNI IQNHDIESAR AEKKVFLLAT KTKHPFLTNL YCSFQTENRI 

       910        920        930        940        950        960 
YFAMEFIGGG DLMWHVQNQR LSVRRAKFYA AEVLLALKYF HDNGVIYRDL KLENILLTPE 

       970        980        990       1000       1010       1020 
GHIKIADYGL CKDEMWYGNR TSTFCGTPEF MAPEILKEQE YTKAVDWWAF GVLLYQMLLC 

      1030       1040       1050       1060       1070       1080 
QSPFSGDDED EVFNAILTDE PLYPIDMAGE IVQIFQGLLT KDPEKRLGAG PRDADEVMEE 

      1090       1100       1110       1120       1130       1140 
PFFRNINFDD ILNLRVKPPY IPEIKSPEDT SYFEQEFTSA PPTLTPLPSV LTTSQQEEFR 

      1150 
GFSFMPDDLD L 

« Hide

References

« Hide 'large scale' references
[1]"A candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle."
Levin D.E., Fields F.O., Kunisawa R., Bishop J.M., Thorner J.
Cell 62:213-224(1990) [PubMed: 2196995] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ATCC 204278 / EG123 / SM1058.
[2]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-577 AND SER-761, MASS SPECTROMETRY.
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226; SER-577; THR-751; SER-761; THR-981; THR-983 AND THR-1125, MASS SPECTROMETRY.
[6]"Saccharomyces cerevisiae cell lysis mutations cly5 and cly7 define temperature-sensitive alleles of PKC1, the gene encoding yeast protein kinase C."
Baymiller J., McCullough J.E.
Yeast 13:305-312(1997) [PubMed: 9133734] [Abstract]
Cited for: VARIANTS CLY5 AND CLY7.
+Additional computationally mapped references.

Cross-references

Sequence databases

M32491 mRNA. Translation: AAA34878.1.
X79489 Genomic DNA. Translation: CAA55990.1.
Z35866 Genomic DNA. Translation: CAA84932.1.
PIRS45390.
RefSeqNP_009445.1.

3D structure databases

HSSPHSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1516N.
IntActP24583. 9 interactions.

Proteomic databases

PeptideAtlasP24583.

Genome annotation databases

EnsemblYBL105C. Saccharomyces cerevisiae. [Contig view]
GeneID852169.
GenomeReviewsGene locus YBL105C in contig Y13134_GR.
KEGGsce:YBL105C.
NMPDRfig|4932.3.peg.130.

Organism-specific databases

CYGDYBL105c.
SGDS000000201. PKC1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP24583.
OMAP24583. WCCHCGY.

Enzyme and pathway databases

BRENDA2.7.11.13. 250.

Gene expression databases

ArrayExpressP24583.
GermOnlineYBL105C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000008. C2_Ca-dep.
IPR002219. DAG_PE_bd.
IPR000861. HR1-like_rho-bd.
IPR011072. HR1_rho-bd.
IPR015745. PKC.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PANTHERPTHR22985:SF86. PKC. 1 hit.
PfamPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF02185. HR1. 2 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00742. Hr1. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. False negative.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio970614.

Entry information

Entry nameKPC1_YEAST
AccessionPrimary (citable) accession number: P24583
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1994
Last modified: June 16, 2009
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents