Reviewed, UniProtKB/Swiss-Prot P24583 (KPC1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein kinase C-like 1
      Short name=PKC 1
    EC=2.7.11.13

Gene names

Name:	PKC1
Synonyms:	HPO2, STT1
Ordered Locus Names:	YBL105C
ORF Names:	YBL0807

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for cell growth and for the G2->M transition of the cell division cycle. Mediates a protein kinase cascade; it activates BCK1 which itself activates MKK1/MKK2.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. Contains 2 REM (Hr1) repeats.

Ontologies

Keywords
Biological process	Cell cycle
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin filament organization Inferred from genetic interaction. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from mutant phenotype. Source: SGD protein amino acid phosphorylation Inferred from direct assay. Source: SGD protein kinase cascade Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD cytoskeleton Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD site of polarized growth Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein kinase C activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ENO1	P00924	1	EBI-9860,EBI-6468

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1151	1151	Protein kinase C-like 1		PRO_0000055738
Regions
	Repeat	5 – 77	73	REM 1
	Repeat	118 – 193	76	REM 2
	Domain	196 – 288	93	C2
	Domain	824 – 1083	260	Protein kinase
	Domain	1084 – 1151	68	AGC-kinase C-terminal
	Zinc finger	414 – 461	48	Phorbol-ester/DAG-type 1
	Zinc finger	481 – 531	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	830 – 838	9	ATP By similarity
Sites
	Active site	949	1	Proton acceptor By similarity
	Binding site	853	1	ATP By similarity
Amino acid modifications
	Modified residue	226	1	Phosphoserine Ref.5
	Modified residue	577	1	Phosphoserine Ref.5 Ref.4
	Modified residue	751	1	Phosphothreonine Ref.5
	Modified residue	761	1	Phosphoserine Ref.5 Ref.4
	Modified residue	981	1	Phosphothreonine Ref.5
	Modified residue	983	1	Phosphothreonine Ref.5
	Modified residue	1125	1	Phosphothreonine Ref.5
Natural variations
	Natural variant	958	1	T → I in cly5; temperature-sensitive mutation that cause cell lysis at high temperature.
	Natural variant	1023	1	P → S in cly7; temperature-sensitive mutation that cause cell lysis at high temperature.
Experimental info
	Sequence conflict	81	1	F → C in AAA34878. Ref.1
	Sequence conflict	244	1	T → S in AAA34878. Ref.1
	Sequence conflict	606	1	G → E in AAA34878. Ref.1
	Sequence conflict	623	1	S → P in AAA34878. Ref.1
	Sequence conflict	789	1	P → A in AAA34878. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24583-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 3300E8D523C8587C	FASTA	1,151	131,519
	10 20 30 40 50 60 MSFSQLEQNI KKKIAVEENI IRGASALKKK TSNVMVIQKC NTNIREARQN LEYLEDSLKK 70 80 90 100 110 120 LRLKTAQQSQ GENGSEDNER FNSKEYGFLS TKSPNEHIFS RLDLVKYDCP SLAQRIQYML 130 140 150 160 170 180 QQLEFKLQVE KQYQEANTKL TKLYQIDGDQ RSSSAAEGGA MESKYRIQML NKALKKYQAI 190 200 210 220 230 240 NVDFDQFKHQ PNDIMDNQQP KFRRKQLTGV LTIGITAARD VDHIQSPMFA RKPESYVTIK 250 260 270 280 290 300 IDDTIKARTK PSRNDRWSED FQIPVEKGNE IEITVYDKVN DSLIPVAIMW LLLSDIAEEI 310 320 330 340 350 360 RKKKAGQTNE QQGWVNASNI NGGSSLASEE GSTLTSTYSN SAIQSTSAKN VQGENTSTSQ 370 380 390 400 410 420 ISTNSWFVLE PSGQILLTLG FHKSSQIERK QLMGGLHRHG AIINRKEEIF EQHGHHFVQK 430 440 450 460 470 480 SFYNIMCCAY CGDFLRYTGF QCQDCKFLCH KKCYTNVVTK CIAKTSTDTD PDEAKLNHRI 490 500 510 520 530 540 PHRFLPTSNR GTKWCCHCGY ILPWGRHKVR KCSECGIMCH AQCAHLVPDF CGMSMEMANK 550 560 570 580 590 600 ILKTIQDTKR NQEKKKRTVP SAQLGSSIGT ANGSDLSPSK LAERANAPLP PQPRKHDKTP 610 620 630 640 650 660 SPQKVGRDSP TKQHDPIIDK KISLQTHGRE KLNKFIDENE AYLNFTEGAQ QTAEFSSPEK 670 680 690 700 710 720 TLDPTSNRRS LGLTDLSIEH SQTWESKDDL MRDELELWKA QREEMELEIK QDSGEIQEDL 730 740 750 760 770 780 EVDHIDLETK QKLDWENKND FREADLTIDS THTNPFRDMN SETFQIEQDH ASKEVLQETV 790 800 810 820 830 840 SLAPTSTHPS RTTDQQSPQK SQTSTSAKHK KRAAKRRKVS LDNFVLLKVL GKGNFGKVIL 850 860 870 880 890 900 SKSKNTDRLC AIKVLKKDNI IQNHDIESAR AEKKVFLLAT KTKHPFLTNL YCSFQTENRI 910 920 930 940 950 960 YFAMEFIGGG DLMWHVQNQR LSVRRAKFYA AEVLLALKYF HDNGVIYRDL KLENILLTPE 970 980 990 1000 1010 1020 GHIKIADYGL CKDEMWYGNR TSTFCGTPEF MAPEILKEQE YTKAVDWWAF GVLLYQMLLC 1030 1040 1050 1060 1070 1080 QSPFSGDDED EVFNAILTDE PLYPIDMAGE IVQIFQGLLT KDPEKRLGAG PRDADEVMEE 1090 1100 1110 1120 1130 1140 PFFRNINFDD ILNLRVKPPY IPEIKSPEDT SYFEQEFTSA PPTLTPLPSV LTTSQQEEFR 1150 GFSFMPDDLD L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle." Levin D.E., Fields F.O., Kunisawa R., Bishop J.M., Thorner J. Cell 62:213-224(1990) [PubMed: 2196995] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 204278 / EG123 / SM1058.
[2]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-577 AND SER-761, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226; SER-577; THR-751; SER-761; THR-981; THR-983 AND THR-1125, MASS SPECTROMETRY.
[6]	"Saccharomyces cerevisiae cell lysis mutations cly5 and cly7 define temperature-sensitive alleles of PKC1, the gene encoding yeast protein kinase C." Baymiller J., McCullough J.E. Yeast 13:305-312(1997) [PubMed: 9133734] [Abstract] Cited for: VARIANTS CLY5 AND CLY7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M32491 mRNA. Translation: AAA34878.1. X79489 Genomic DNA. Translation: CAA55990.1. Z35866 Genomic DNA. Translation: CAA84932.1.
PIR	S45390.
RefSeq	NP_009445.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1516N.
IntAct	P24583. 9 interactions.
Proteomic databases
PeptideAtlas	P24583.
Genome annotation databases
Ensembl	YBL105C. Saccharomyces cerevisiae. [Contig view]
GeneID	852169.
GenomeReviews	Gene locus YBL105C in contig Y13134_GR.
KEGG	sce:YBL105C.
NMPDR	fig|4932.3.peg.130.
Organism-specific databases
CYGD	YBL105c.
SGD	S000000201. PKC1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P24583.
OMA	P24583. WCCHCGY.
Enzyme and pathway databases
BRENDA	2.7.11.13. 250.
Gene expression databases
ArrayExpress	P24583.
GermOnline	YBL105C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR002219. DAG_PE_bd. IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR015745. PKC. IPR017892. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF02185. HR1. 2 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00742. Hr1. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. False negative. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970614.

Entry information

Entry name

KPC1_YEAST

Accession

Primary (citable) accession number: P24583

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)