Reviewed, UniProtKB/Swiss-Prot P24582 (MTN3_NEILA)

Names and origin

Protein names

Recommended name:
    Modification methylase NlaIII
      Short name=M.NlaIII
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase NlaIII

Gene names

Name:

nlaIIIM

Organism

Neisseria lactamica

Taxonomic identifier

486 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.
Caution	It is uncertain whether Met-1 or Met-57 is the initiator.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Modification methylase NlaIII		PRO_0000087968

Sequences

	Sequence		Length	Mass (Da)
	P24582-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 8BCF1A1A35F02E89	FASTA	334	38,382
	10 20 30 40 50 60 MNYIGSKLKL SNWLETEISN VAGHSLSDKV FCDLFAGTGI VGRKFKTNVK QVIANDMEYY 70 80 90 100 110 120 SYVLNRNYIG NCQSILKAGE LLQRLEQLPP REGLIYQHYC LGSGSERQYF SDENGKKIDA 130 140 150 160 170 180 VRIQIEEWKN TRYIDEDTYY FLLATLLEGA DKVANTASVY GAFLKNLKKS ALKPLSLEPA 190 200 210 220 230 240 LFEIGSDGHQ VYQADANQLI KNISGDILYL DPPYNARQYG ANYHLLNSIA LYDDFTPKGK 250 260 270 280 290 300 TGLREYSRSK YCSKSDVVPV FEALIRDADF QYIFLSYNNE GLMSVGQVRE IFERFGKYDL 310 320 330 VQTEYRRFKA DKTENRNHKA NSTFEYLHIL EKTF

References

[1]

"Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase." Labbe D., Hoeltke H.J., Lau P.C.K. Mol. Gen. Genet. 224:101-110(1990) [PubMed: 2277628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54485 Genomic DNA. Translation: CAA38356.1.
PIR	XYNHAL. S12036.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3468. M.NlaIII.
Enzyme and pathway databases
BRENDA	2.1.1.72. 96206.
Family and domain databases
InterPro	IPR002294. D12N6_MeTrfase. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTN3_NEILA

Accession

Primary (citable) accession number: P24582

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	September 22, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)