Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24582

- MTN3_NEILA

UniProt

P24582 - MTN3_NEILA

Protein

Modification methylase NlaIII

Gene

nlaIIIM

Organism
Neisseria lactamica
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase NlaIII (EC:2.1.1.72)
    Short name:
    M.NlaIII
    Alternative name(s):
    Adenine-specific methyltransferase NlaIII
    Gene namesi
    Name:nlaIIIM
    OrganismiNeisseria lactamica
    Taxonomic identifieri486 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 334334Modification methylase NlaIIIPRO_0000087968Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24582-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYIGSKLKL SNWLETEISN VAGHSLSDKV FCDLFAGTGI VGRKFKTNVK    50
    QVIANDMEYY SYVLNRNYIG NCQSILKAGE LLQRLEQLPP REGLIYQHYC 100
    LGSGSERQYF SDENGKKIDA VRIQIEEWKN TRYIDEDTYY FLLATLLEGA 150
    DKVANTASVY GAFLKNLKKS ALKPLSLEPA LFEIGSDGHQ VYQADANQLI 200
    KNISGDILYL DPPYNARQYG ANYHLLNSIA LYDDFTPKGK TGLREYSRSK 250
    YCSKSDVVPV FEALIRDADF QYIFLSYNNE GLMSVGQVRE IFERFGKYDL 300
    VQTEYRRFKA DKTENRNHKA NSTFEYLHIL EKTF 334
    Length:334
    Mass (Da):38,382
    Last modified:March 1, 1992 - v1
    Checksum:i8BCF1A1A35F02E89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54485 Genomic DNA. Translation: CAA38356.1.
    PIRiS12036. XYNHAL.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54485 Genomic DNA. Translation: CAA38356.1 .
    PIRi S12036. XYNHAL.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase."
      Labbe D., Hoeltke H.J., Lau P.C.K.
      Mol. Gen. Genet. 224:101-110(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

    Entry informationi

    Entry nameiMTN3_NEILA
    AccessioniPrimary (citable) accession number: P24582
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-57 is the initiator.Curated

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3