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P24582

- MTN3_NEILA

UniProt

P24582 - MTN3_NEILA

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Protein
Modification methylase NlaIII
Gene
nlaIIIM
Organism
Neisseria lactamica
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NlaIII (EC:2.1.1.72)
Short name:
M.NlaIII
Alternative name(s):
Adenine-specific methyltransferase NlaIII
Gene namesi
Name:nlaIIIM
OrganismiNeisseria lactamica
Taxonomic identifieri486 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Modification methylase NlaIII
PRO_0000087968Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24582-1 [UniParc]FASTAAdd to Basket

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MNYIGSKLKL SNWLETEISN VAGHSLSDKV FCDLFAGTGI VGRKFKTNVK    50
QVIANDMEYY SYVLNRNYIG NCQSILKAGE LLQRLEQLPP REGLIYQHYC 100
LGSGSERQYF SDENGKKIDA VRIQIEEWKN TRYIDEDTYY FLLATLLEGA 150
DKVANTASVY GAFLKNLKKS ALKPLSLEPA LFEIGSDGHQ VYQADANQLI 200
KNISGDILYL DPPYNARQYG ANYHLLNSIA LYDDFTPKGK TGLREYSRSK 250
YCSKSDVVPV FEALIRDADF QYIFLSYNNE GLMSVGQVRE IFERFGKYDL 300
VQTEYRRFKA DKTENRNHKA NSTFEYLHIL EKTF 334
Length:334
Mass (Da):38,382
Last modified:March 1, 1992 - v1
Checksum:i8BCF1A1A35F02E89
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54485 Genomic DNA. Translation: CAA38356.1.
PIRiS12036. XYNHAL.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54485 Genomic DNA. Translation: CAA38356.1 .
PIRi S12036. XYNHAL.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase."
    Labbe D., Hoeltke H.J., Lau P.C.K.
    Mol. Gen. Genet. 224:101-110(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Entry informationi

Entry nameiMTN3_NEILA
AccessioniPrimary (citable) accession number: P24582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-57 is the initiator.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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