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Reviewed, UniProtKB/Swiss-Prot P24582 (MTN3_NEILA)

Last modified September 22, 2009. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Modification methylase NlaIII
      Short name=M.NlaIII
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase NlaIII
Gene names
Name: nlaIIIM
OrganismNeisseria lactamica
Taxonomic identifier486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Caution

It is uncertain whether Met-1 or Met-57 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Modification methylase NlaIII
PRO_0000087968

Sequences

Sequence LengthMass (Da)Tools
P24582-1 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 8BCF1A1A35F02E89

FASTA33438,382
        10         20         30         40         50         60 
MNYIGSKLKL SNWLETEISN VAGHSLSDKV FCDLFAGTGI VGRKFKTNVK QVIANDMEYY 

        70         80         90        100        110        120 
SYVLNRNYIG NCQSILKAGE LLQRLEQLPP REGLIYQHYC LGSGSERQYF SDENGKKIDA 

       130        140        150        160        170        180 
VRIQIEEWKN TRYIDEDTYY FLLATLLEGA DKVANTASVY GAFLKNLKKS ALKPLSLEPA 

       190        200        210        220        230        240 
LFEIGSDGHQ VYQADANQLI KNISGDILYL DPPYNARQYG ANYHLLNSIA LYDDFTPKGK 

       250        260        270        280        290        300 
TGLREYSRSK YCSKSDVVPV FEALIRDADF QYIFLSYNNE GLMSVGQVRE IFERFGKYDL 

       310        320        330 
VQTEYRRFKA DKTENRNHKA NSTFEYLHIL EKTF 

« Hide

References

[1]"Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase."
Labbe D., Hoeltke H.J., Lau P.C.K.
Mol. Gen. Genet. 224:101-110(1990) [PubMed: 2277628] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Cross-references

Sequence databases

X54485 Genomic DNA. Translation: CAA38356.1.
PIRXYNHAL. S12036.

3D structure databases

ModBaseSearch...

Protein family/group databases

REBASE3468. M.NlaIII.

Enzyme and pathway databases

BRENDA2.1.1.72. 96206.

Family and domain databases

InterProIPR002294. D12N6_MeTrfase.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSPR00505. D12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTN3_NEILA
AccessionPrimary (citable) accession number: P24582
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: September 22, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents