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Protein

Modification methylase NlaIII

Gene

nlaIIIM

Organism
Neisseria lactamica
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NlaIII (EC:2.1.1.72)
Short name:
M.NlaIII
Alternative name(s):
Adenine-specific methyltransferase NlaIII
Gene namesi
Name:nlaIIIM
OrganismiNeisseria lactamica
Taxonomic identifieri486 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Modification methylase NlaIIIPRO_0000087968Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYIGSKLKL SNWLETEISN VAGHSLSDKV FCDLFAGTGI VGRKFKTNVK
60 70 80 90 100
QVIANDMEYY SYVLNRNYIG NCQSILKAGE LLQRLEQLPP REGLIYQHYC
110 120 130 140 150
LGSGSERQYF SDENGKKIDA VRIQIEEWKN TRYIDEDTYY FLLATLLEGA
160 170 180 190 200
DKVANTASVY GAFLKNLKKS ALKPLSLEPA LFEIGSDGHQ VYQADANQLI
210 220 230 240 250
KNISGDILYL DPPYNARQYG ANYHLLNSIA LYDDFTPKGK TGLREYSRSK
260 270 280 290 300
YCSKSDVVPV FEALIRDADF QYIFLSYNNE GLMSVGQVRE IFERFGKYDL
310 320 330
VQTEYRRFKA DKTENRNHKA NSTFEYLHIL EKTF
Length:334
Mass (Da):38,382
Last modified:March 1, 1992 - v1
Checksum:i8BCF1A1A35F02E89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54485 Genomic DNA. Translation: CAA38356.1.
PIRiS12036. XYNHAL.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54485 Genomic DNA. Translation: CAA38356.1.
PIRiS12036. XYNHAL.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase."
    Labbe D., Hoeltke H.J., Lau P.C.K.
    Mol. Gen. Genet. 224:101-110(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Entry informationi

Entry nameiMTN3_NEILA
AccessioniPrimary (citable) accession number: P24582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: January 7, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-57 is the initiator.Curated

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.