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P24582 (MTN3_NEILA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NlaIII

Short name=M.NlaIII
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase NlaIII
Gene names
Name:nlaIIIM
OrganismNeisseria lactamica
Taxonomic identifier486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Caution

It is uncertain whether Met-1 or Met-57 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Modification methylase NlaIII
PRO_0000087968

Sequences

Sequence LengthMass (Da)Tools
P24582 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 8BCF1A1A35F02E89

FASTA33438,382
        10         20         30         40         50         60 
MNYIGSKLKL SNWLETEISN VAGHSLSDKV FCDLFAGTGI VGRKFKTNVK QVIANDMEYY 

        70         80         90        100        110        120 
SYVLNRNYIG NCQSILKAGE LLQRLEQLPP REGLIYQHYC LGSGSERQYF SDENGKKIDA 

       130        140        150        160        170        180 
VRIQIEEWKN TRYIDEDTYY FLLATLLEGA DKVANTASVY GAFLKNLKKS ALKPLSLEPA 

       190        200        210        220        230        240 
LFEIGSDGHQ VYQADANQLI KNISGDILYL DPPYNARQYG ANYHLLNSIA LYDDFTPKGK 

       250        260        270        280        290        300 
TGLREYSRSK YCSKSDVVPV FEALIRDADF QYIFLSYNNE GLMSVGQVRE IFERFGKYDL 

       310        320        330 
VQTEYRRFKA DKTENRNHKA NSTFEYLHIL EKTF 

« Hide

References

[1]"Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase."
Labbe D., Hoeltke H.J., Lau P.C.K.
Mol. Gen. Genet. 224:101-110(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54485 Genomic DNA. Translation: CAA38356.1.
PIRXYNHAL. S12036.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTN3_NEILA
AccessionPrimary (citable) accession number: P24582
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries