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P24581 (MTNX_NEILA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosine-specific methyltransferase NlaX

Short name=M.NlaX
EC=2.1.1.37
Gene names
Name:nlaXM
OrganismNeisseria lactamica
Taxonomic identifier486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Cytosine-specific methyltransferase NlaX
PRO_0000087911

Regions

Domain2 – 308307SAM-dependent MTase C5-type

Sites

Active site741 By similarity

Sequences

Sequence LengthMass (Da)Tools
P24581 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: E75150C89ABEA611

FASTA31334,843
        10         20         30         40         50         60 
MFKIIDLFAG IGGIRLGFEQ AFDDVRCVFS SEIDKYAVQT YQANHGGETV CGDITQTDVA 

        70         80         90        100        110        120 
DIPDHDILSA GFPCQPFSQA GLKKGFADTR GTLFFDIERI LLAKKPQAFL LENVKQLKGH 

       130        140        150        160        170        180 
DKGRTLQVIL AHLQQAGYKV YTEVLKARDF GIPQNRERIY LVGFLNHDVD FRFPQPIGQA 

       190        200        210        220        230        240 
TAVGDILEAY PDEKYTISDK LWQGYQRRKA ENRAAGKGFG YGLFNAESAY TNTISARYYK 

       250        260        270        280        290        300 
DGSEILIEQP GKNPRKITPP EAARLQGFPD SFQIPVSDAQ AYRQFGNSVC VPVIRAIAEQ 

       310 
MKAALSAVSD RKV 

« Hide

References

[1]"Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase."
Labbe D., Hoeltke H.J., Lau P.C.K.
Mol. Gen. Genet. 224:101-110(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54485 Genomic DNA. Translation: CAA38357.1.
PIRXYNHCL. S12037.

3D structure databases

ProteinModelPortalP24581.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2158. M.NlaX.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTNX_NEILA
AccessionPrimary (citable) accession number: P24581
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries