Reviewed, UniProtKB/Swiss-Prot P24581 (MTNX_NEILA)

Names and origin

Protein names

Recommended name:
    Cytosine-specific methyltransferase NlaX
      Short name=M.NlaX
    EC=2.1.1.37

Gene names

Name:

nlaXM

Organism

Neisseria lactamica

Taxonomic identifier

486 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Cytosine-specific methyltransferase NlaX		PRO_0000087911
Sites
	Active site	74	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24581-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: E75150C89ABEA611	FASTA	313	34,843
	10 20 30 40 50 60 MFKIIDLFAG IGGIRLGFEQ AFDDVRCVFS SEIDKYAVQT YQANHGGETV CGDITQTDVA 70 80 90 100 110 120 DIPDHDILSA GFPCQPFSQA GLKKGFADTR GTLFFDIERI LLAKKPQAFL LENVKQLKGH 130 140 150 160 170 180 DKGRTLQVIL AHLQQAGYKV YTEVLKARDF GIPQNRERIY LVGFLNHDVD FRFPQPIGQA 190 200 210 220 230 240 TAVGDILEAY PDEKYTISDK LWQGYQRRKA ENRAAGKGFG YGLFNAESAY TNTISARYYK 250 260 270 280 290 300 DGSEILIEQP GKNPRKITPP EAARLQGFPD SFQIPVSDAQ AYRQFGNSVC VPVIRAIAEQ 310 MKAALSAVSD RKV

References

[1]

"Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase." Labbe D., Hoeltke H.J., Lau P.C.K. Mol. Gen. Genet. 224:101-110(1990) [PubMed: 2277628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54485 Genomic DNA. Translation: CAA38357.1.
PIR	XYNHCL. S12037.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	2158. M.NlaX.
Enzyme and pathway databases
BRENDA	2.1.1.37. 96206.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTNX_NEILA

Accession

Primary (citable) accession number: P24581

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	March 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)