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P24571 (MASY_CUCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase, glyoxysomal

EC=2.3.3.9
OrganismCucurbita maxima (Pumpkin) (Winter squash)
Taxonomic identifier3661 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionTransferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566Malate synthase, glyoxysomal
PRO_0000166867

Regions

Motif564 – 5663Microbody targeting signal Potential

Sites

Active site1821Proton acceptor By similarity
Active site4671Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P24571 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: B77F77D1D593834A

FASTA56664,636
        10         20         30         40         50         60 
MGSLGMYSES AVRKKSSRGY DVPEGVDIRG RYDEEFARIL NKEALLFVAD LQRTFRNHIR 

        70         80         90        100        110        120 
YSMECRREAK RRYNEGAVPG FDPATKYIRE SEWTCASVPP AVADRRVEIT GPVERKMIIN 

       130        140        150        160        170        180 
ALNSGAKVFM ADFEDALSPN WENLMRGQIN LKDAVDGTIS FHDKARNKVY KLNDQTAKLF 

       190        200        210        220        230        240 
VRPRGWHFAE AHIFIDGEPA TGCLVDFGLY FFHNHANFRR SQGQGSGPFF YLPKMEHSRE 

       250        260        270        280        290        300 
AKIWNSVFER AEKMAGIERG SIRATVLIET LPAVFQMDEI LYELRDHSVG LNCGRWDYIF 

       310        320        330        340        350        360 
SYVKTFQAHL DRLLPDRVQV GMAQHFMRSY SDLLIRTCHT VVCHVGGMAA QIPIRDDPKA 

       370        380        390        400        410        420 
NEMALELVRK DKLREAKAGH DGTWAAHPGL IPACMEVFTN SMGNAPNQIR SARRDDAANL 

       430        440        450        460        470        480 
TEDDLLQQPR GVRTLEGLRL NTRVGIQYLA AWLTGTGSVP LYNLMEDAAT AEISRVQNWQ 

       490        500        510        520        530        540 
WLKYGVELDG DGLGVRVNKE LFARVVEEEM ERIEREVGKE KFRKGMYKEA CKMFTRQCTA 

       550        560 
PTLDDFLTLD AYNHIVIHHP RELSRL 

« Hide

References

[1]"Pumpkin malate synthase. Cloning and sequencing of the cDNA and northern blot analysis."
Mori H., Takeda-Yoshikawa Y., Hara-Nishimura I., Nishimura M.
Eur. J. Biochem. 197:331-336(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 83-91; 197-206 AND 417-426.
Strain: cv. Kurokawa Amakuri Nankin.
Tissue: Etiolated cotyledon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56948 mRNA. Translation: CAA40262.1.

3D structure databases

ProteinModelPortalP24571.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP24571.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_CUCMA
AccessionPrimary (citable) accession number: P24571
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways