Reviewed, UniProtKB/Swiss-Prot P24571 (MASY_CUCMA)

Names and origin

Protein names	Recommended name:     Malate synthase, glyoxysomal     EC=2.3.3.9
Organism	Cucurbita maxima (Pumpkin) (Winter squash)
Taxonomic identifier	3661 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Cucurbita

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Malate synthase, glyoxysomal		PRO_0000166867
Regions
	Motif	564 – 566	3	Microbody targeting signal Potential
Sites
	Active site	182	1	Proton acceptor By similarity
	Active site	467	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24571-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: B77F77D1D593834A	FASTA	566	64,636
	10 20 30 40 50 60 MGSLGMYSES AVRKKSSRGY DVPEGVDIRG RYDEEFARIL NKEALLFVAD LQRTFRNHIR 70 80 90 100 110 120 YSMECRREAK RRYNEGAVPG FDPATKYIRE SEWTCASVPP AVADRRVEIT GPVERKMIIN 130 140 150 160 170 180 ALNSGAKVFM ADFEDALSPN WENLMRGQIN LKDAVDGTIS FHDKARNKVY KLNDQTAKLF 190 200 210 220 230 240 VRPRGWHFAE AHIFIDGEPA TGCLVDFGLY FFHNHANFRR SQGQGSGPFF YLPKMEHSRE 250 260 270 280 290 300 AKIWNSVFER AEKMAGIERG SIRATVLIET LPAVFQMDEI LYELRDHSVG LNCGRWDYIF 310 320 330 340 350 360 SYVKTFQAHL DRLLPDRVQV GMAQHFMRSY SDLLIRTCHT VVCHVGGMAA QIPIRDDPKA 370 380 390 400 410 420 NEMALELVRK DKLREAKAGH DGTWAAHPGL IPACMEVFTN SMGNAPNQIR SARRDDAANL 430 440 450 460 470 480 TEDDLLQQPR GVRTLEGLRL NTRVGIQYLA AWLTGTGSVP LYNLMEDAAT AEISRVQNWQ 490 500 510 520 530 540 WLKYGVELDG DGLGVRVNKE LFARVVEEEM ERIEREVGKE KFRKGMYKEA CKMFTRQCTA 550 560 PTLDDFLTLD AYNHIVIHHP RELSRL

References

[1]

"Pumpkin malate synthase. Cloning and sequencing of the cDNA and northern blot analysis." Mori H., Takeda-Yoshikawa Y., Hara-Nishimura I., Nishimura M. Eur. J. Biochem. 197:331-336(1991) [PubMed: 1709098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 83-91; 197-206 AND 417-426. Strain: cv. Kurokawa Amakuri Nankin. Tissue: Etiolated cotyledon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56948 mRNA. Translation: CAA40262.1.
3D structure databases
SMR	P24571. Positions 126-541.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 2176.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_CUCMA

Accession

Primary (citable) accession number: P24571

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)