Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Napin-1A

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The small, basic, water-soluble napins are one of the two major kinds of storage proteins synthesized in the seed during its maturation.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
Napin-1A
Alternative name(s):
Napin BnIa
Cleaved into the following 2 chains:
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000321311 – 31Napin-1A small chainAdd BLAST31
ChainiPRO_000003213232 – 110Napin-1A large chainAdd BLAST79

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi5 ↔ 56Interchain (between small and large chains)1 Publication
Disulfide bondi18 ↔ 45Interchain (between small and large chains)1 Publication
Modified residuei32Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi46 ↔ 931 Publication
Disulfide bondi58 ↔ 1011 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

The mature protein consists of a small and a large chain linked by disulfide bonds.

Structurei

Secondary structure

1110
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Helixi19 – 24Combined sources6
Helixi43 – 50Combined sources8
Helixi54 – 56Combined sources3
Helixi58 – 69Combined sources12
Helixi71 – 73Combined sources3
Helixi79 – 85Combined sources7
Turni96 – 98Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PNBNMR-A1-31[»]
B32-106[»]
ProteinModelPortaliP24565.
SMRiP24565.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24565.

Family & Domainsi

Sequence similaritiesi

Belongs to the 2S seed storage albumins family.Curated

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QPQKCQREFQ QEQHLRACQQ WIRQQLAGSP FQSGPQEGPW LREQCCNELY
60 70 80 90 100
QEDQVCVCPT LKQAAKSVRV QGQHGPFQST RIYQIAKNLP NVCNMKQIGT
110
CPFIAIPFFP
Length:110
Mass (Da):12,691
Last modified:March 1, 1992 - v1
Checksum:i3A2938ADA2C1E995
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-adjacent residuesi31 – 32Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti31Missing in minor form SM, less than 7%. 1
Natural varianti37E → Q in napin-1B. 1
Natural varianti107 – 110Missing in napin-1B. 4

Sequence databases

PIRiS20350.
S26636.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

PIRiS20350.
S26636.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PNBNMR-A1-31[»]
B32-106[»]
ProteinModelPortaliP24565.
SMRiP24565.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP24565.

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei2SSI_BRANA
AccessioniPrimary (citable) accession number: P24565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Napin 1A and 1B are minor component of the seed 2S albumin.
The sequence shown is that of napin IA.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.