P24565 (2SSI_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Napin-1A Alternative name(s): Napin BnIa Cleaved into the following 2 chains: Napin-1A small chain Napin-1A large chain
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The small, basic, water-soluble napins are one of the two major kinds of storage proteins synthesized in the seed during its maturation.
Subunit structure	The mature protein consists of a small and a large chain linked by disulfide bonds.
Miscellaneous	Napin 1A and 1B are minor component of the seed 2S albumin. The sequence shown is that of napin IA.
Sequence similarities	Belongs to the 2S seed storage albumins family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 31

31

Napin-1A small chain

PRO_0000032131

Chain

32 – 110

79

Napin-1A large chain

PRO_0000032132

Amino acid modifications

Modified residue

32

1

Pyrrolidone carboxylic acid

Disulfide bond

5 ↔ 56

Interchain (between small and large chains) Ref.2

Disulfide bond

18 ↔ 45

Interchain (between small and large chains) Ref.2

Disulfide bond

46 ↔ 93

Ref.2

Disulfide bond

58 ↔ 101

Ref.2

Natural variations

Natural variant

31

1

Missing in minor form SM, less than 7%.

Natural variant

37

1

E → Q in napin-1B.

Natural variant

107 – 110

4

Missing in napin-1B.

Experimental info

Non-adjacent residues

31 – 32

2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

110

Helix Strand Turn

Details...

Helix

3 – 12

10

Helix

19 – 24

6

Helix

43 – 50

8

Helix

54 – 56

3

Helix

58 – 69

12

Helix

71 – 73

3

Helix

79 – 85

7

Turn

96 – 98

3

Sequences

	Sequence		Length	Mass (Da)
	P24565 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 3A2938ADA2C1E995	FASTA	110	12,691
	10 20 30 40 50 60 QPQKCQREFQ QEQHLRACQQ WIRQQLAGSP FQSGPQEGPW LREQCCNELY QEDQVCVCPT 70 80 90 100 110 LKQAAKSVRV QGQHGPFQST RIYQIAKNLP NVCNMKQIGT CPFIAIPFFP

References

[1]	"A new distinct group of 2 S albumins from rapeseed. Amino acid sequence of two low molecular weight napins." Monsalve R.I., Lopez-Otin C., Villalba M., Rodriguez R. FEBS Lett. 295:207-210(1991) [PubMed: 1765156] [Abstract] Cited for: PROTEIN SEQUENCE, PYROGLUTAMATE FORMATION AT GLN-32. Tissue: Seed.
[2]	"1H NMR assignment and global fold of napin BnIb, a representative 2S albumin seed protein." Rico M., Bruix M., Gonzalez C., Monsalve R.I., Rodriguez R. Biochemistry 35:15672-15682(1996) [PubMed: 8961930] [Abstract] Cited for: STRUCTURE BY NMR OF 1-106, DISULFIDE BONDS.

Cross-references

Sequence databases

PIR

S20350.
S26636.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PNB	NMR	-	A	1-31	[»]
			B	32-106	[»]

ProteinModelPortal

P24565.

SMR

P24565. Positions 1-106.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]

Gene3D

G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

PRINTS

PR00496. NAPIN.

SMART

SM00499. AAI. 1 hit.
[Graphical view]

SUPFAM

SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.

ProtoNet

Search...

Entry information

Entry name

2SSI_BRANA

Accession

Primary (citable) accession number: P24565

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 5, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program