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Protein

Napin-1A

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The small, basic, water-soluble napins are one of the two major kinds of storage proteins synthesized in the seed during its maturation.

Miscellaneous

Napin 1A and 1B are minor component of the seed 2S albumin.
The sequence shown is that of napin IA.

GO - Molecular functioni

Keywordsi

Molecular functionSeed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
Napin-1A
Alternative name(s):
Napin BnIa
Cleaved into the following 2 chains:
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000321311 – 31Napin-1A small chainAdd BLAST31
ChainiPRO_000003213232 – 110Napin-1A large chainAdd BLAST79

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi5 ↔ 56Interchain (between small and large chains)1 Publication
Disulfide bondi18 ↔ 45Interchain (between small and large chains)1 Publication
Modified residuei32Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi46 ↔ 931 Publication
Disulfide bondi58 ↔ 1011 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

The mature protein consists of a small and a large chain linked by disulfide bonds.

Structurei

Secondary structure

1110
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Helixi19 – 24Combined sources6
Helixi43 – 50Combined sources8
Helixi54 – 56Combined sources3
Helixi58 – 69Combined sources12
Helixi71 – 73Combined sources3
Helixi79 – 85Combined sources7
Turni96 – 98Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PNBNMR-A1-31[»]
B32-106[»]
ProteinModelPortaliP24565.
SMRiP24565.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24565.

Family & Domainsi

Sequence similaritiesi

Belongs to the 2S seed storage albumins family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
PfamiView protein in Pfam
PF00234. Tryp_alpha_amyl. 1 hit.
PRINTSiPR00496. NAPIN.
SMARTiView protein in SMART
SM00499. AAI. 1 hit.
SUPFAMiSSF47699. SSF47699. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QPQKCQREFQ QEQHLRACQQ WIRQQLAGSP FQSGPQEGPW LREQCCNELY
60 70 80 90 100
QEDQVCVCPT LKQAAKSVRV QGQHGPFQST RIYQIAKNLP NVCNMKQIGT
110
CPFIAIPFFP
Length:110
Mass (Da):12,691
Last modified:March 1, 1992 - v1
Checksum:i3A2938ADA2C1E995
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-adjacent residuesi31 – 32Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti31Missing in minor form SM, less than 7%. 1
Natural varianti37E → Q in napin-1B. 1
Natural varianti107 – 110Missing in napin-1B. 4

Sequence databases

PIRiS20350.
S26636.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry namei2SSI_BRANA
AccessioniPrimary (citable) accession number: P24565
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 12, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families