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Protein

Twitching mobility protein

Gene

pilT

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in pilus retraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi130 – 1378ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • pilus retraction Source: PseudoCAP
  • transport Source: UniProtKB-KW
  • type IV pilus-dependent motility Source: PseudoCAP
Complete GO annotation...

Keywords - Biological processi

Fimbrium biogenesis, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.15.2.1. the outer membrane protein secreting main terminal branch (mtb) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Twitching mobility protein
Gene namesi
Name:pilT
Ordered Locus Names:PA0395
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0395.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • type IV pilus Source: PseudoCAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Twitching mobility proteinPRO_0000207296Add
BLAST

Proteomic databases

PaxDbiP24559.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0395.

Structurei

Secondary structure

1
344
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1210Combined sources
Beta strandi16 – 216Combined sources
Beta strandi27 – 304Combined sources
Beta strandi33 – 364Combined sources
Helixi44 – 5411Combined sources
Helixi57 – 6610Combined sources
Beta strandi69 – 757Combined sources
Turni76 – 783Combined sources
Beta strandi79 – 8810Combined sources
Beta strandi91 – 999Combined sources
Turni106 – 1105Combined sources
Helixi113 – 1208Combined sources
Beta strandi122 – 1298Combined sources
Helixi136 – 15015Combined sources
Beta strandi154 – 1618Combined sources
Beta strandi169 – 1779Combined sources
Turni178 – 1803Combined sources
Beta strandi181 – 1833Combined sources
Helixi185 – 1928Combined sources
Beta strandi198 – 2036Combined sources
Helixi208 – 21912Combined sources
Beta strandi223 – 2297Combined sources
Helixi233 – 24210Combined sources
Helixi246 – 25914Combined sources
Beta strandi260 – 26910Combined sources
Beta strandi277 – 2848Combined sources
Helixi287 – 2959Combined sources
Helixi298 – 3003Combined sources
Helixi301 – 3066Combined sources
Helixi309 – 3113Combined sources
Helixi316 – 3205Combined sources
Helixi331 – 3366Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JVUX-ray3.10A/B/C1-344[»]
3JVVX-ray2.60A/B/C1-344[»]
ProteinModelPortaliP24559.
SMRiP24559. Positions 1-342.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24559.

Family & Domainsi

Sequence similaritiesi

Belongs to the GSP E family.Curated

Phylogenomic databases

eggNOGiENOG4105CZX. Bacteria.
COG2805. LUCA.
InParanoidiP24559.
KOiK02669.
OMAiVSPMIRV.
OrthoDBiEOG6JMMWM.
PhylomeDBiP24559.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR006321. PilT.
IPR001482. T2SS_protein-E.
[Graphical view]
PfamiPF00437. T2SSE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01420. pilT_fam. 1 hit.
PROSITEiPS00662. T2SP_E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24559-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDITELLAFS AKQGASDLHL SAGLPPMIRV DGDVRRINLP PLEHKQVHAL
60 70 80 90 100
IYDIMNDKQR KDFEEFLETD FSFEVPGVAR FRVNAFNQNR GAGAVFRTIP
110 120 130 140 150
SKVLTMEELG MGEVFKRVSD VPRGLVLVTG PTGSGKSTTL AAMLDYLNNT
160 170 180 190 200
KYHHILTIED PIEFVHESKK CLVNQREVHR DTLGFSEALR SALREDPDII
210 220 230 240 250
LVGEMRDLET IRLALTAAET GHLVFGTLHT TSAAKTIDRV VDVFPAEEKA
260 270 280 290 300
MVRSMLSESL QSVISQTLIK KIGGGRVAAH EIMIGTPAIR NLIREDKVAQ
310 320 330 340
MYSAIQTGGS LGMQTLDMCL KGLVAKGLIS RENAREKAKI PENF
Length:344
Mass (Da):38,021
Last modified:March 1, 1992 - v1
Checksum:iC35F89F28AD0BE01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55524 Genomic DNA. Translation: AAA25963.1.
AE004091 Genomic DNA. Translation: AAG03784.1.
PIRiJN0055.
RefSeqiNP_249086.1. NC_002516.2.
WP_003084552.1. NZ_ASJY01000077.1.

Genome annotation databases

EnsemblBacteriaiAAG03784; AAG03784; PA0395.
GeneIDi878389.
KEGGipae:PA0395.
PATRICi19835030. VBIPseAer58763_0416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55524 Genomic DNA. Translation: AAA25963.1.
AE004091 Genomic DNA. Translation: AAG03784.1.
PIRiJN0055.
RefSeqiNP_249086.1. NC_002516.2.
WP_003084552.1. NZ_ASJY01000077.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JVUX-ray3.10A/B/C1-344[»]
3JVVX-ray2.60A/B/C1-344[»]
ProteinModelPortaliP24559.
SMRiP24559. Positions 1-342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0395.

Protein family/group databases

TCDBi3.A.15.2.1. the outer membrane protein secreting main terminal branch (mtb) family.

Proteomic databases

PaxDbiP24559.

Protocols and materials databases

DNASUi878389.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03784; AAG03784; PA0395.
GeneIDi878389.
KEGGipae:PA0395.
PATRICi19835030. VBIPseAer58763_0416.

Organism-specific databases

PseudoCAPiPA0395.

Phylogenomic databases

eggNOGiENOG4105CZX. Bacteria.
COG2805. LUCA.
InParanoidiP24559.
KOiK02669.
OMAiVSPMIRV.
OrthoDBiEOG6JMMWM.
PhylomeDBiP24559.

Miscellaneous databases

EvolutionaryTraceiP24559.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR006321. PilT.
IPR001482. T2SS_protein-E.
[Graphical view]
PfamiPF00437. T2SSE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01420. pilT_fam. 1 hit.
PROSITEiPS00662. T2SP_E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria."
    Whitchurch C.B., Hobbs M., Livingston S.P., Krishnapillai V., Mattick J.S.
    Gene 101:33-44(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiPILT_PSEAE
AccessioniPrimary (citable) accession number: P24559
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 11, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.