P24555 (PTRB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protease 2
EC=3.4.21.83

Alternative name(s):
Oligopeptidase B
Protease II

Gene names

Name:	ptrB
Synonyms:	tlp
Ordered Locus Names:	b1845, JW1834

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues.
Catalytic activity	Hydrolysis of -Arg-|-Xaa- and -Lys-|-Xaa- bonds in oligopeptides, even when P1' residue is proline.
Sequence similarities	Belongs to the peptidase S9A family.

Ontologies

Keywords
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 240839. Source: EcoCyc
Molecular_function	serine-type endopeptidase activity Inferred from direct assay Ref.1. Source: EcoCyc serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Protease 2		PRO_0000122404
Sites
	Active site	532	1	Charge relay system Ref.1
	Active site	617	1	Charge relay system By similarity
	Active site	652	1	Charge relay system By similarity
Experimental info
	Sequence conflict	682 – 686	5	ATPAD → LRLRTKYFPDNVSVLNAAPG SCCPGY AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24555 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B41AD388044D56B2	FASTA	686	79,491
	10 20 30 40 50 60 MLPKAARIPH AMTLHGDTRI DNYYWLRDDT RSQPEVLDYL QQENSYGHRV MASQQALQDR 70 80 90 100 110 120 ILKEIIDRIP QREVSAPYIK NGYRYRHIYE PGCEYAIYQR QSAFSEEWDE WETLLDANKR 130 140 150 160 170 180 AAHSEFYSMG GMAITPDNTI MALAEDFLSR RQYGIRFRNL ETGNWYPELL DNVEPSFVWA 190 200 210 220 230 240 NDSWIFYYVR KHPVTLLPYQ VWRHAIGTPA SQDKLIYEEK DDTYYVSLHK TTSKHYVVIH 250 260 270 280 290 300 LASATTSEVR LLDAEMADAE PFVFLPRRKD HEYSLDHYQH RFYLRSNRHG KNFGLYRTRM 310 320 330 340 350 360 RDEQQWEELI PPRENIMLEG FTLFTDWLVV EERQRGLTSL RQINRKTREV IGIAFDDPAY 370 380 390 400 410 420 VTWIAYNPEP ETARLRYGYS SMTTPDTLFE LDMDTGERRV LKQTEVPGFY AANYRSEHLW 430 440 450 460 470 480 IVARDGVEVP VSLVYHRKHF RKGHNPLLVY GYGSYGASID ADFSFSRLSL LDRGFVYAIV 490 500 510 520 530 540 HVRGGGELGQ QWYEDGKFLK KKNTFNDYLD ACDALLKLGY GSPSLCYAMG GSAGGMLMGV 550 560 570 580 590 600 AINQRPELFH GVIAQVPFVD VVTTMLDESI PLTTGEFEEW GNPQDPQYYE YMKSYSPYDN 610 620 630 640 650 660 VTAQAYPHLL VTTGLHDSQV QYWEPAKWVA KLRELKTDDH LLLLCTDMDS GHGGKSGRFK 670 680 SYEGVAMEYA FLVALAQGTL PATPAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme." Kanatani A., Masuda T., Shimoda T., Misoka F., Lin X.S., Yoshimoto T., Tsuru D. J. Biochem. 110:315-320(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 33694 / HB101.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10976 Genomic DNA. Translation: BAA01750.1. U00096 Genomic DNA. Translation: AAC74915.1. AP009048 Genomic DNA. Translation: BAA15651.1.
PIR	E64946.
RefSeq	NP_416359.1. NC_000913.2. YP_490107.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P24555.
SMR	P24555. Positions 3-680.
ModBase	Search...
Protein-protein interaction databases
IntAct	P24555. 3 interactions.
STRING	511145.b1845.
Protein family/group databases
MEROPS	S09.010.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74915; AAC74915; b1845. BAA15651; BAA15651; BAA15651.
GeneID	12934150. 946358.
KEGG	ecj:Y75_p1821. eco:b1845.
PATRIC	32119011. VBIEscCol129921_1923.
Organism-specific databases
EchoBASE	EB0997.
EcoGene	EG11004. ptrB.
Phylogenomic databases
eggNOG	COG1770.
HOGENOM	HOG000249811.
KO	K01354.
OMA	PLYQYGY.
ProtClustDB	PRK10115.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11004-MONOMER. ECOL316407:JW1834-MONOMER. MetaCyc:EG11004-MONOMER.
Gene expression databases
Genevestigator	P24555.
Family and domain databases
Gene3D	2.130.10.120. 1 hit.
InterPro	IPR002471. Pept_S9_AS. IPR023302. Pept_S9A_oligopept_N. IPR001375. Peptidase_S9. IPR002470. Peptidase_S9A. IPR004106. Peptidase_S9A_B_C_N. [Graphical view]
PANTHER	PTHR11757. PTHR11757. 1 hit.
Pfam	PF00326. Peptidase_S9. 1 hit. PF02897. Peptidase_S9_N. 1 hit. [Graphical view]
PRINTS	PR00862. PROLIGOPTASE.
SUPFAM	SSF50993. Peptidase_S9A_N. 1 hit.
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTRB_ECOLI

Accession

Primary (citable) accession number: P24555
Secondary accession number(s): P78068

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program