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Protein

Protease 2

Gene

ptrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues.

Catalytic activityi

Hydrolysis of -Arg-|-Xaa- and -Lys-|-Xaa- bonds in oligopeptides, even when P1' residue is proline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei532 – 5321Charge relay systemPROSITE-ProRule annotation1 Publication
Active sitei617 – 6171Charge relay systemPROSITE-ProRule annotation
Active sitei652 – 6521Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  • serine-type endopeptidase activity Source: EcoCyc
  • serine-type exopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG11004-MONOMER.
ECOL316407:JW1834-MONOMER.
MetaCyc:EG11004-MONOMER.
RETL1328306-WGS:GSTH-1229-MONOMER.
BRENDAi3.4.21.83. 2026.

Protein family/group databases

ESTHERiecoli-ptrb. S9N_PREPL_Peptidase_S9.
MEROPSiS09.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease 2 (EC:3.4.21.83)
Alternative name(s):
Oligopeptidase B
Protease II
Gene namesi
Name:ptrB
Synonyms:tlp
Ordered Locus Names:b1845, JW1834
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11004. ptrB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Protease 2PRO_0000122404Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP24555. 3 interactions.
STRINGi511145.b1845.

Structurei

3D structure databases

ProteinModelPortaliP24555.
SMRiP24555. Positions 19-673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Curated

Phylogenomic databases

eggNOGiCOG1770.
HOGENOMiHOG000249811.
InParanoidiP24555.
KOiK01354.
OMAiYAYMKSY.
OrthoDBiEOG6DVJMH.
PhylomeDBiP24555.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPKAARIPH AMTLHGDTRI DNYYWLRDDT RSQPEVLDYL QQENSYGHRV
60 70 80 90 100
MASQQALQDR ILKEIIDRIP QREVSAPYIK NGYRYRHIYE PGCEYAIYQR
110 120 130 140 150
QSAFSEEWDE WETLLDANKR AAHSEFYSMG GMAITPDNTI MALAEDFLSR
160 170 180 190 200
RQYGIRFRNL ETGNWYPELL DNVEPSFVWA NDSWIFYYVR KHPVTLLPYQ
210 220 230 240 250
VWRHAIGTPA SQDKLIYEEK DDTYYVSLHK TTSKHYVVIH LASATTSEVR
260 270 280 290 300
LLDAEMADAE PFVFLPRRKD HEYSLDHYQH RFYLRSNRHG KNFGLYRTRM
310 320 330 340 350
RDEQQWEELI PPRENIMLEG FTLFTDWLVV EERQRGLTSL RQINRKTREV
360 370 380 390 400
IGIAFDDPAY VTWIAYNPEP ETARLRYGYS SMTTPDTLFE LDMDTGERRV
410 420 430 440 450
LKQTEVPGFY AANYRSEHLW IVARDGVEVP VSLVYHRKHF RKGHNPLLVY
460 470 480 490 500
GYGSYGASID ADFSFSRLSL LDRGFVYAIV HVRGGGELGQ QWYEDGKFLK
510 520 530 540 550
KKNTFNDYLD ACDALLKLGY GSPSLCYAMG GSAGGMLMGV AINQRPELFH
560 570 580 590 600
GVIAQVPFVD VVTTMLDESI PLTTGEFEEW GNPQDPQYYE YMKSYSPYDN
610 620 630 640 650
VTAQAYPHLL VTTGLHDSQV QYWEPAKWVA KLRELKTDDH LLLLCTDMDS
660 670 680
GHGGKSGRFK SYEGVAMEYA FLVALAQGTL PATPAD
Length:686
Mass (Da):79,491
Last modified:November 1, 1997 - v2
Checksum:iB41AD388044D56B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti682 – 6865ATPAD → LRLRTKYFPDNVSVLNAAPG SCCPGY AA sequence (PubMed:1769955).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10976 Genomic DNA. Translation: BAA01750.1.
U00096 Genomic DNA. Translation: AAC74915.1.
AP009048 Genomic DNA. Translation: BAA15651.1.
PIRiE64946.
RefSeqiNP_416359.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74915; AAC74915; b1845.
BAA15651; BAA15651; BAA15651.
GeneIDi946358.
KEGGiecj:Y75_p1821.
eco:b1845.
PATRICi32119011. VBIEscCol129921_1923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10976 Genomic DNA. Translation: BAA01750.1.
U00096 Genomic DNA. Translation: AAC74915.1.
AP009048 Genomic DNA. Translation: BAA15651.1.
PIRiE64946.
RefSeqiNP_416359.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP24555.
SMRiP24555. Positions 19-673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24555. 3 interactions.
STRINGi511145.b1845.

Protein family/group databases

ESTHERiecoli-ptrb. S9N_PREPL_Peptidase_S9.
MEROPSiS09.010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74915; AAC74915; b1845.
BAA15651; BAA15651; BAA15651.
GeneIDi946358.
KEGGiecj:Y75_p1821.
eco:b1845.
PATRICi32119011. VBIEscCol129921_1923.

Organism-specific databases

EchoBASEiEB0997.
EcoGeneiEG11004. ptrB.

Phylogenomic databases

eggNOGiCOG1770.
HOGENOMiHOG000249811.
InParanoidiP24555.
KOiK01354.
OMAiYAYMKSY.
OrthoDBiEOG6DVJMH.
PhylomeDBiP24555.

Enzyme and pathway databases

BioCyciEcoCyc:EG11004-MONOMER.
ECOL316407:JW1834-MONOMER.
MetaCyc:EG11004-MONOMER.
RETL1328306-WGS:GSTH-1229-MONOMER.
BRENDAi3.4.21.83. 2026.

Miscellaneous databases

PROiP24555.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme."
    Kanatani A., Masuda T., Shimoda T., Misoka F., Lin X.S., Yoshimoto T., Tsuru D.
    J. Biochem. 110:315-320(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 33694 / HB101.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPTRB_ECOLI
AccessioniPrimary (citable) accession number: P24555
Secondary accession number(s): P78068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.