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P24555 (PTRB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protease 2

EC=3.4.21.83
Alternative name(s):
Oligopeptidase B
Protease II
Gene names
Name:ptrB
Synonyms:tlp
Ordered Locus Names:b1845, JW1834
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length686 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues.

Catalytic activity

Hydrolysis of -Arg-|-Xaa- and -Lys-|-Xaa- bonds in oligopeptides, even when P1' residue is proline.

Sequence similarities

Belongs to the peptidase S9A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 686686Protease 2
PRO_0000122404

Sites

Active site5321Charge relay system Ref.1
Active site6171Charge relay system By similarity
Active site6521Charge relay system By similarity

Experimental info

Sequence conflict682 – 6865ATPAD → LRLRTKYFPDNVSVLNAAPG SCCPGY AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24555 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: B41AD388044D56B2

FASTA68679,491
        10         20         30         40         50         60 
MLPKAARIPH AMTLHGDTRI DNYYWLRDDT RSQPEVLDYL QQENSYGHRV MASQQALQDR 

        70         80         90        100        110        120 
ILKEIIDRIP QREVSAPYIK NGYRYRHIYE PGCEYAIYQR QSAFSEEWDE WETLLDANKR 

       130        140        150        160        170        180 
AAHSEFYSMG GMAITPDNTI MALAEDFLSR RQYGIRFRNL ETGNWYPELL DNVEPSFVWA 

       190        200        210        220        230        240 
NDSWIFYYVR KHPVTLLPYQ VWRHAIGTPA SQDKLIYEEK DDTYYVSLHK TTSKHYVVIH 

       250        260        270        280        290        300 
LASATTSEVR LLDAEMADAE PFVFLPRRKD HEYSLDHYQH RFYLRSNRHG KNFGLYRTRM 

       310        320        330        340        350        360 
RDEQQWEELI PPRENIMLEG FTLFTDWLVV EERQRGLTSL RQINRKTREV IGIAFDDPAY 

       370        380        390        400        410        420 
VTWIAYNPEP ETARLRYGYS SMTTPDTLFE LDMDTGERRV LKQTEVPGFY AANYRSEHLW 

       430        440        450        460        470        480 
IVARDGVEVP VSLVYHRKHF RKGHNPLLVY GYGSYGASID ADFSFSRLSL LDRGFVYAIV 

       490        500        510        520        530        540 
HVRGGGELGQ QWYEDGKFLK KKNTFNDYLD ACDALLKLGY GSPSLCYAMG GSAGGMLMGV 

       550        560        570        580        590        600 
AINQRPELFH GVIAQVPFVD VVTTMLDESI PLTTGEFEEW GNPQDPQYYE YMKSYSPYDN 

       610        620        630        640        650        660 
VTAQAYPHLL VTTGLHDSQV QYWEPAKWVA KLRELKTDDH LLLLCTDMDS GHGGKSGRFK 

       670        680 
SYEGVAMEYA FLVALAQGTL PATPAD 

« Hide

References

« Hide 'large scale' references
[1]"Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme."
Kanatani A., Masuda T., Shimoda T., Misoka F., Lin X.S., Yoshimoto T., Tsuru D.
J. Biochem. 110:315-320(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 33694 / HB101.
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10976 Genomic DNA. Translation: BAA01750.1.
U00096 Genomic DNA. Translation: AAC74915.1.
AP009048 Genomic DNA. Translation: BAA15651.1.
PIRE64946.
RefSeqNP_416359.1. NC_000913.3.
YP_490107.1. NC_007779.1.

3D structure databases

ProteinModelPortalP24555.
SMRP24555. Positions 19-673.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP24555. 3 interactions.
STRING511145.b1845.

Protein family/group databases

MEROPSS09.010.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74915; AAC74915; b1845.
BAA15651; BAA15651; BAA15651.
GeneID12934150.
946358.
KEGGecj:Y75_p1821.
eco:b1845.
PATRIC32119011. VBIEscCol129921_1923.

Organism-specific databases

EchoBASEEB0997.
EcoGeneEG11004. ptrB.

Phylogenomic databases

eggNOGCOG1770.
HOGENOMHOG000249811.
KOK01354.
OMASLDPWFS.
OrthoDBEOG6DVJMH.
PhylomeDBP24555.

Enzyme and pathway databases

BioCycEcoCyc:EG11004-MONOMER.
ECOL316407:JW1834-MONOMER.
MetaCyc:EG11004-MONOMER.
RETL1328306-WGS:GSTH-1229-MONOMER.

Gene expression databases

GenevestigatorP24555.

Family and domain databases

Gene3D2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERPTHR11757. PTHR11757. 1 hit.
PfamPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSPR00862. PROLIGOPTASE.
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP24555.

Entry information

Entry namePTRB_ECOLI
AccessionPrimary (citable) accession number: P24555
Secondary accession number(s): P78068
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene