Reviewed, UniProtKB/Swiss-Prot P24555 (PTRB_ECOLI)

Names and origin

Protein names

Recommended name:
    Protease 2
    EC=3.4.21.83
Alternative name(s):
    Protease II
    Oligopeptidase B

Gene names

Name:	ptrB
Synonyms:	tlp
Ordered Locus Names:	b1845, JW1834

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues.
Catalytic activity	Hydrolysis of -Arg-|-Xaa- and -Lys-|-Xaa- bonds in oligopeptides, even when P1' residue is proline.
Sequence similarities	Belongs to the peptidase S9A family.

Ontologies

Keywords
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Protease 2		PRO_0000122404
Sites
	Active site	532	1	Charge relay system Ref.1
	Active site	617	1	Charge relay system By similarity
	Active site	652	1	Charge relay system By similarity
Experimental info
	Sequence conflict	682 – 686	5	ATPAD → LRLRTKYFPDNVSVLNAAPG SCCPGY AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24555-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B41AD388044D56B2	FASTA	686	79,491
	10 20 30 40 50 60 MLPKAARIPH AMTLHGDTRI DNYYWLRDDT RSQPEVLDYL QQENSYGHRV MASQQALQDR 70 80 90 100 110 120 ILKEIIDRIP QREVSAPYIK NGYRYRHIYE PGCEYAIYQR QSAFSEEWDE WETLLDANKR 130 140 150 160 170 180 AAHSEFYSMG GMAITPDNTI MALAEDFLSR RQYGIRFRNL ETGNWYPELL DNVEPSFVWA 190 200 210 220 230 240 NDSWIFYYVR KHPVTLLPYQ VWRHAIGTPA SQDKLIYEEK DDTYYVSLHK TTSKHYVVIH 250 260 270 280 290 300 LASATTSEVR LLDAEMADAE PFVFLPRRKD HEYSLDHYQH RFYLRSNRHG KNFGLYRTRM 310 320 330 340 350 360 RDEQQWEELI PPRENIMLEG FTLFTDWLVV EERQRGLTSL RQINRKTREV IGIAFDDPAY 370 380 390 400 410 420 VTWIAYNPEP ETARLRYGYS SMTTPDTLFE LDMDTGERRV LKQTEVPGFY AANYRSEHLW 430 440 450 460 470 480 IVARDGVEVP VSLVYHRKHF RKGHNPLLVY GYGSYGASID ADFSFSRLSL LDRGFVYAIV 490 500 510 520 530 540 HVRGGGELGQ QWYEDGKFLK KKNTFNDYLD ACDALLKLGY GSPSLCYAMG GSAGGMLMGV 550 560 570 580 590 600 AINQRPELFH GVIAQVPFVD VVTTMLDESI PLTTGEFEEW GNPQDPQYYE YMKSYSPYDN 610 620 630 640 650 660 VTAQAYPHLL VTTGLHDSQV QYWEPAKWVA KLRELKTDDH LLLLCTDMDS GHGGKSGRFK 670 680 SYEGVAMEYA FLVALAQGTL PATPAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme." Kanatani A., Masuda T., Shimoda T., Misoka F., Lin X.S., Yoshimoto T., Tsuru D. J. Biochem. 110:315-320(1991) [PubMed: 1769955] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 33694 / HB101.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10976 Genomic DNA. Translation: BAA01750.1. U00096 Genomic DNA. Translation: AAC74915.1. AP009048 Genomic DNA. Translation: BAA15651.1.
PIR	E64946.
RefSeq	AP_002465.1. NP_416359.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	946358.
GenomeReviews	Gene locus JW1834 in contig AP009048_GR. Gene locus b1845 in contig U00096_GR.
KEGG	ecj:JW1834. eco:b1845.
Organism-specific databases
EchoBASE	EB0997.
EcoGene	EG11004. ptrB.
CMR	Search...
Phylogenomic databases
HOGENOM	P24555.
OMA	P24555. TRVLYVE.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11004-MON.
Family and domain databases
InterPro	IPR002471. Pept_S9_AS. IPR001375. Peptidase_S9. IPR002470. Peptidase_S9A. IPR004106. Peptidase_S9A_N. [Graphical view]
PANTHER	PTHR11757. Peptidase_S9A. 1 hit.
Pfam	PF00326. Peptidase_S9. 1 hit. PF02897. Peptidase_S9_N. 1 hit. [Graphical view]
PRINTS	PR00862. PROLIGOPTASE.
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTRB_ECOLI

Accession

Primary (citable) accession number: P24555
Secondary accession number(s): P78068

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)