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P24555

- PTRB_ECOLI

UniProt

P24555 - PTRB_ECOLI

Protein

Protease 2

Gene

ptrB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues.

    Catalytic activityi

    Hydrolysis of -Arg-|-Xaa- and -Lys-|-Xaa- bonds in oligopeptides, even when P1' residue is proline.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei532 – 5321Charge relay system1 PublicationPROSITE-ProRule annotation
    Active sitei617 – 6171Charge relay systemPROSITE-ProRule annotation
    Active sitei652 – 6521Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: EcoCyc
    2. serine-type exopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11004-MONOMER.
    ECOL316407:JW1834-MONOMER.
    MetaCyc:EG11004-MONOMER.
    RETL1328306-WGS:GSTH-1229-MONOMER.

    Protein family/group databases

    MEROPSiS09.010.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protease 2 (EC:3.4.21.83)
    Alternative name(s):
    Oligopeptidase B
    Protease II
    Gene namesi
    Name:ptrB
    Synonyms:tlp
    Ordered Locus Names:b1845, JW1834
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11004. ptrB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 686686Protease 2PRO_0000122404Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP24555.

    Interactioni

    Protein-protein interaction databases

    IntActiP24555. 3 interactions.
    STRINGi511145.b1845.

    Structurei

    3D structure databases

    ProteinModelPortaliP24555.
    SMRiP24555. Positions 19-673.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9A family.Curated

    Phylogenomic databases

    eggNOGiCOG1770.
    HOGENOMiHOG000249811.
    KOiK01354.
    OMAiSLDPWFS.
    OrthoDBiEOG6DVJMH.
    PhylomeDBiP24555.

    Family and domain databases

    Gene3Di2.130.10.120. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR023302. Pept_S9A_N.
    IPR001375. Peptidase_S9.
    IPR002470. Peptidase_S9A.
    [Graphical view]
    PANTHERiPTHR11757. PTHR11757. 1 hit.
    PfamiPF00326. Peptidase_S9. 1 hit.
    PF02897. Peptidase_S9_N. 1 hit.
    [Graphical view]
    PRINTSiPR00862. PROLIGOPTASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24555-1 [UniParc]FASTAAdd to Basket

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    MLPKAARIPH AMTLHGDTRI DNYYWLRDDT RSQPEVLDYL QQENSYGHRV    50
    MASQQALQDR ILKEIIDRIP QREVSAPYIK NGYRYRHIYE PGCEYAIYQR 100
    QSAFSEEWDE WETLLDANKR AAHSEFYSMG GMAITPDNTI MALAEDFLSR 150
    RQYGIRFRNL ETGNWYPELL DNVEPSFVWA NDSWIFYYVR KHPVTLLPYQ 200
    VWRHAIGTPA SQDKLIYEEK DDTYYVSLHK TTSKHYVVIH LASATTSEVR 250
    LLDAEMADAE PFVFLPRRKD HEYSLDHYQH RFYLRSNRHG KNFGLYRTRM 300
    RDEQQWEELI PPRENIMLEG FTLFTDWLVV EERQRGLTSL RQINRKTREV 350
    IGIAFDDPAY VTWIAYNPEP ETARLRYGYS SMTTPDTLFE LDMDTGERRV 400
    LKQTEVPGFY AANYRSEHLW IVARDGVEVP VSLVYHRKHF RKGHNPLLVY 450
    GYGSYGASID ADFSFSRLSL LDRGFVYAIV HVRGGGELGQ QWYEDGKFLK 500
    KKNTFNDYLD ACDALLKLGY GSPSLCYAMG GSAGGMLMGV AINQRPELFH 550
    GVIAQVPFVD VVTTMLDESI PLTTGEFEEW GNPQDPQYYE YMKSYSPYDN 600
    VTAQAYPHLL VTTGLHDSQV QYWEPAKWVA KLRELKTDDH LLLLCTDMDS 650
    GHGGKSGRFK SYEGVAMEYA FLVALAQGTL PATPAD 686
    Length:686
    Mass (Da):79,491
    Last modified:November 1, 1997 - v2
    Checksum:iB41AD388044D56B2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti682 – 6865ATPAD → LRLRTKYFPDNVSVLNAAPG SCCPGY AA sequence (PubMed:1769955)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10976 Genomic DNA. Translation: BAA01750.1.
    U00096 Genomic DNA. Translation: AAC74915.1.
    AP009048 Genomic DNA. Translation: BAA15651.1.
    PIRiE64946.
    RefSeqiNP_416359.1. NC_000913.3.
    YP_490107.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74915; AAC74915; b1845.
    BAA15651; BAA15651; BAA15651.
    GeneIDi12934150.
    946358.
    KEGGiecj:Y75_p1821.
    eco:b1845.
    PATRICi32119011. VBIEscCol129921_1923.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10976 Genomic DNA. Translation: BAA01750.1 .
    U00096 Genomic DNA. Translation: AAC74915.1 .
    AP009048 Genomic DNA. Translation: BAA15651.1 .
    PIRi E64946.
    RefSeqi NP_416359.1. NC_000913.3.
    YP_490107.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P24555.
    SMRi P24555. Positions 19-673.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P24555. 3 interactions.
    STRINGi 511145.b1845.

    Protein family/group databases

    MEROPSi S09.010.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74915 ; AAC74915 ; b1845 .
    BAA15651 ; BAA15651 ; BAA15651 .
    GeneIDi 12934150.
    946358.
    KEGGi ecj:Y75_p1821.
    eco:b1845.
    PATRICi 32119011. VBIEscCol129921_1923.

    Organism-specific databases

    EchoBASEi EB0997.
    EcoGenei EG11004. ptrB.

    Phylogenomic databases

    eggNOGi COG1770.
    HOGENOMi HOG000249811.
    KOi K01354.
    OMAi SLDPWFS.
    OrthoDBi EOG6DVJMH.
    PhylomeDBi P24555.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11004-MONOMER.
    ECOL316407:JW1834-MONOMER.
    MetaCyc:EG11004-MONOMER.
    RETL1328306-WGS:GSTH-1229-MONOMER.

    Miscellaneous databases

    PROi P24555.

    Gene expression databases

    Genevestigatori P24555.

    Family and domain databases

    Gene3Di 2.130.10.120. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR023302. Pept_S9A_N.
    IPR001375. Peptidase_S9.
    IPR002470. Peptidase_S9A.
    [Graphical view ]
    PANTHERi PTHR11757. PTHR11757. 1 hit.
    Pfami PF00326. Peptidase_S9. 1 hit.
    PF02897. Peptidase_S9_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00862. PROLIGOPTASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme."
      Kanatani A., Masuda T., Shimoda T., Misoka F., Lin X.S., Yoshimoto T., Tsuru D.
      J. Biochem. 110:315-320(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 33694 / HB101.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPTRB_ECOLI
    AccessioniPrimary (citable) accession number: P24555
    Secondary accession number(s): P78068
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3