P24552 (OXDA_FUSSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: D-amino-acid oxidase Short name=DAAO Short name=DAMOX Short name=DAO EC=1.4.3.3
Organism	Fusarium solani subsp. pisi (Nectria haematococca)
Taxonomic identifier	70791 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Nectria › Nectria haematococca complex

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme can effectively convert cephalosporin C into 7-beta-(5-carboxy-5-oxopentanamido)-cephalosporinic acid.
Catalytic activity	A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD.
Subcellular location	Peroxisome Potential.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the DAMOX/DASOX family.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-amino-acid oxidase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	D-amino-acid oxidase		PRO_0000162766
Regions
	Nucleotide binding	5 – 19	15	FAD By similarity
	Nucleotide binding	34 – 35	2	FAD By similarity
	Nucleotide binding	45 – 46	2	FAD By similarity
	Nucleotide binding	50 – 52	3	FAD By similarity
	Nucleotide binding	332 – 336	5	FAD By similarity
	Motif	359 – 361	3	Microbody targeting signal Potential
Sites
	Binding site	172	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	195	1	FAD By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	305	1	Substrate By similarity
	Binding site	333	1	Substrate; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24552 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 148119480E2A67A5	FASTA	361	39,696
	10 20 30 40 50 60 MSNTIVVVGA GVIGLTSALL LSKNKGNKIT VVAKHMPGDY DVEYASPFAG ANHSPMATEE 70 80 90 100 110 120 SSEWERRTWY EFKRLVEEVP EAGVHFQKSR IQRRNVDTEK AQRSGFPDAL FSKEPWFKNM 130 140 150 160 170 180 FEDFREQHPS EVIPGYDSGC EFTSVCINTA IYLPWLLGQC IKNGVIVKRA ILNDISEAKK 190 200 210 220 230 240 LSHAGKTPNI IVNATGLGSY KLGGVEDKTM APARGQIVVV RNESSPMLLT SGVEDGGADV 250 260 270 280 290 300 MYLMQRAAGG GTILGGTYDV GNWESQPDPN IANRIMQRIV EVRPEIANGK GVKGLSVIRH 310 320 330 340 350 360 AVGMRPWRKD GVRIEEEKLD DETWIVHNYG HSGWGYQGSY GCAENVVQLV DKVGKAAKSK L

References

[1]	"Structure and expression of cDNA for D-amino acid oxidase active against cephalosporin C from Fusarium solani." Isogai T., Ono H., Ishitani Y., Kojo H., Ueda Y., Kohsaka M. J. Biochem. 108:1063-1069(1990) [PubMed: 1982443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: M-0718 / FERM P-2688.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00809 mRNA. Translation: BAA00692.1.
PIR	JX0152.
3D structure databases
ProteinModelPortal	P24552.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR023209. D-aa_oxidase. IPR006181. D-amino_acid_oxidase_CS. IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 3 hits.
PANTHER	PTHR11530. PTHR11530. 1 hit.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PIRSF	PIRSF000189. D-aa_oxidase. 1 hit.
PROSITE	PS00677. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OXDA_FUSSO

Accession

Primary (citable) accession number: P24552

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program