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Protein

Retinal dehydrogenase 1

Gene

Aldh1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In addition to the activity on acetaldehyde and related substrates, is also involved in the oxidation of aldehydes derived from biogenic amines such as epinephrine and norepinephrine, as well as the aldehydes generated via lipid peroxidation. Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).By similarity

Catalytic activityi

Retinal + NAD+ + H2O = retinoate + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei170 – 1701Transition state stabilizerBy similarity
Active sitei269 – 2691Proton acceptorPROSITE-ProRule annotation
Active sitei303 – 3031NucleophilePROSITE-ProRule annotation
Binding sitei456 – 4561NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 2516NADBy similarity

GO - Molecular functioni

  1. 3-chloroallyl aldehyde dehydrogenase activity Source: MGI
  2. aldehyde dehydrogenase (NAD) activity Source: MGI
  3. benzaldehyde dehydrogenase (NAD+) activity Source: GO_Central
  4. retinal dehydrogenase activity Source: GO_Central

GO - Biological processi

  1. 9-cis-retinoic acid biosynthetic process Source: MGI
  2. embryonic eye morphogenesis Source: MGI
  3. kidney development Source: Ensembl
  4. liver development Source: Ensembl
  5. midgut development Source: Ensembl
  6. optic cup morphogenesis involved in camera-type eye development Source: MGI
  7. positive regulation of apoptotic process Source: MGI
  8. protein homotetramerization Source: Ensembl
  9. response to drug Source: MGI
  10. response to estradiol Source: Ensembl
  11. response to ethanol Source: Ensembl
  12. response to oxidative stress Source: Ensembl
  13. response to retinoic acid Source: Ensembl
  14. retinoic acid metabolic process Source: MGI
  15. retinol metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.36. 3474.
ReactomeiREACT_309926. Ethanol oxidation.
REACT_310468. RA biosynthesis pathway.
SABIO-RKP24549.
UniPathwayiUPA00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinal dehydrogenase 1 (EC:1.2.1.36)
Short name:
RALDH 1
Short name:
RalDH1
Alternative name(s):
ALDH-E1
ALHDII
Aldehyde dehydrogenase family 1 member A1
Aldehyde dehydrogenase, cytosolic
Gene namesi
Name:Aldh1a1
Synonyms:Ahd-2, Ahd2, Aldh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1353450. Aldh1a1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular vesicular exosome Source: MGI
  3. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 501500Retinal dehydrogenase 1PRO_0000056417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei91 – 911N6-acetyllysineBy similarity
Modified residuei128 – 1281N6-acetyllysineBy similarity
Modified residuei252 – 2521N6-acetyllysineBy similarity
Modified residuei337 – 3371PhosphothreonineBy similarity
Modified residuei353 – 3531N6-acetyllysineBy similarity
Modified residuei367 – 3671N6-acetyllysineBy similarity
Modified residuei410 – 4101N6-acetyllysineBy similarity
Modified residuei413 – 4131PhosphoserineBy similarity
Modified residuei419 – 4191N6-acetyllysineBy similarity
Modified residuei435 – 4351N6-acetyllysineBy similarity
Modified residuei495 – 4951N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP24549.
PaxDbiP24549.
PRIDEiP24549.

2D gel databases

REPRODUCTION-2DPAGEIPI00626662.
P24549.
SWISS-2DPAGEP24549.

PTM databases

PhosphoSiteiP24549.

Expressioni

Tissue specificityi

Expressed in the liver, lung, and testis. Apparently not expressed at detectable levels in kidney, stomach, ovary, heart, and brain.

Gene expression databases

BgeeiP24549.
CleanExiMM_ALDH1A1.
GenevestigatoriP24549.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP24549. 4 interactions.
MINTiMINT-1859401.

Structurei

3D structure databases

ProteinModelPortaliP24549.
SMRiP24549. Positions 9-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 3056Antabuse binding

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP24549.
KOiK07249.
OMAiVEFAHIG.
OrthoDBiEOG7PS1F7.
PhylomeDBiP24549.
TreeFamiTF300455.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPAQPAVP APLADLKIQH TKIFINNEWH NSVSGKKFPV LNPATEEVIC
60 70 80 90 100
HVEEGDKADV DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLMERDR
110 120 130 140 150
LLLATMEALN GGKVFANAYL SDLGGCIKAL KYCAGWADKI HGQTIPSDGD
160 170 180 190 200
IFTYTRREPI GVCGQIIPWN FPMLMFIWKI GPALSCGNTV VVKPAEQTPL
210 220 230 240 250
TALHLASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD KVAFTGSTQV
260 270 280 290 300
GKLIKEAAGK SNLKRVTLEL GGKSPCIVFA DADLDIAVEF AHHGVFYHQG
310 320 330 340 350
QCCVAASRIF VEESVYDEFV KRSVERAKKY VLGNPLTPGI NQGPQIDKEQ
360 370 380 390 400
HDKILDLIES GKKEGAKLEC GGGRWGNKGF FVQPTVFSNV TDEMRIAKEE
410 420 430 440 450
IFGPVQQIMK FKSVDDVIKR ANNTTYGLAA GLFTKDLDKA ITVSSALQAG
460 470 480 490 500
VVWVNCYMML SAQCPFGGFK MSGNGRELGE HGLYEYTELK TVAMKISQKN

S
Length:501
Mass (Da):54,468
Last modified:January 23, 2007 - v5
Checksum:i0E428151B799BD1D
GO

Sequence cautioni

The sequence AAH44729.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81A → R in AAA37202 (PubMed:2055490).Curated
Sequence conflicti8 – 81A → R in AAA37203 (PubMed:2055490).Curated
Sequence conflicti45 – 451T → S in AAB32754 (PubMed:7993664).Curated
Sequence conflicti51 – 511H → Q in AAB32754 (PubMed:7993664).Curated
Sequence conflicti87 – 871R → C in AAA37202 (PubMed:2055490).Curated
Sequence conflicti140 – 1401I → Y AA sequence (PubMed:1935685).Curated
Sequence conflicti458 – 4581M → I in AAA37202 (PubMed:2055490).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74570 mRNA. Translation: AAA37202.1.
M74571 Genomic DNA. Translation: AAA37203.1.
S75713 mRNA. Translation: AAB32754.2.
S77047 Genomic DNA. No translation available.
BC044729 mRNA. Translation: AAH44729.1. Different initiation.
BC054386 mRNA. Translation: AAH54386.1.
CCDSiCCDS29695.1.
PIRiJQ1004.
RefSeqiNP_038495.2. NM_013467.3.
UniGeneiMm.250866.

Genome annotation databases

EnsembliENSMUST00000087638; ENSMUSP00000084918; ENSMUSG00000053279.
GeneIDi11668.
KEGGimmu:11668.
UCSCiuc008gym.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74570 mRNA. Translation: AAA37202.1.
M74571 Genomic DNA. Translation: AAA37203.1.
S75713 mRNA. Translation: AAB32754.2.
S77047 Genomic DNA. No translation available.
BC044729 mRNA. Translation: AAH44729.1. Different initiation.
BC054386 mRNA. Translation: AAH54386.1.
CCDSiCCDS29695.1.
PIRiJQ1004.
RefSeqiNP_038495.2. NM_013467.3.
UniGeneiMm.250866.

3D structure databases

ProteinModelPortaliP24549.
SMRiP24549. Positions 9-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24549. 4 interactions.
MINTiMINT-1859401.

PTM databases

PhosphoSiteiP24549.

2D gel databases

REPRODUCTION-2DPAGEIPI00626662.
P24549.
SWISS-2DPAGEP24549.

Proteomic databases

MaxQBiP24549.
PaxDbiP24549.
PRIDEiP24549.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087638; ENSMUSP00000084918; ENSMUSG00000053279.
GeneIDi11668.
KEGGimmu:11668.
UCSCiuc008gym.1. mouse.

Organism-specific databases

CTDi216.
MGIiMGI:1353450. Aldh1a1.

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP24549.
KOiK07249.
OMAiVEFAHIG.
OrthoDBiEOG7PS1F7.
PhylomeDBiP24549.
TreeFamiTF300455.

Enzyme and pathway databases

UniPathwayiUPA00912.
BRENDAi1.2.1.36. 3474.
ReactomeiREACT_309926. Ethanol oxidation.
REACT_310468. RA biosynthesis pathway.
SABIO-RKP24549.

Miscellaneous databases

NextBioi279287.
PROiP24549.
SOURCEiSearch...

Gene expression databases

BgeeiP24549.
CleanExiMM_ALDH1A1.
GenevestigatoriP24549.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a cytosolic aldehyde dehydrogenase-encoding cDNA from mouse liver."
    Rongnoparut P., Weaver S.
    Gene 101:261-265(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: BALB/c and C57BL/6.
    Tissue: Liver.
  2. "DNA sequence analysis of the cytosolic acetaldehyde dehydrogenase gene (Ahd-2) in mouse strains with variable ethanol preferences."
    Bond S.L., Singh S.M.
    Biochem. Med. Metab. Biol. 52:155-159(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/ReJ, BALB/c and C57BL/6J.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Liver.
  4. "Aldehyde dehydrogenase is a positional marker in the retina."
    McCaffery P., Tempst P., Lara G., Drager U.C.
    Development 112:693-702(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-52; 140-156; 211-230; 309-320; 330-349; 400-410; 421-435 AND 477-490.
    Tissue: Embryonic retina.

Entry informationi

Entry nameiAL1A1_MOUSE
AccessioniPrimary (citable) accession number: P24549
Secondary accession number(s): Q7TQJ0, Q811J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 148 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.