Reviewed,
UniProtKB/Swiss-Prot P24549 (AL1A1_MOUSE)
Last modified
November 3, 2009.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Retinal dehydrogenase 1 Short name=RALDH 1 Short name=RalDH1 EC=1.2.1.36 Alternative name(s): Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase, cytosolic ALHDII ALDH-E1 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 501 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | In addition to the activity on acetaldehyde and related substrates, is also involved in the oxidation of aldehydes derived from biogenic amines such as epinephrine and norepinephrine, as well as the aldehydes generated via lipid peroxidation. Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid By similarity. |
| Catalytic activity | Retinal + NAD+ + H2O = retinoate + NADH. |
| Pathway | |
| Subunit structure | Homodimer. |
| Subcellular location | |
| Tissue specificity | Expressed in the liver, lung, and testis. Apparently not expressed at detectable levels in kidney, stomach, ovary, heart, and brain. |
| Sequence similarities | Belongs to the aldehyde dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Acetylation |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | 9-cis-retinoic acid biosynthetic process Inferred from direct assay. Source: MGI optic cup morphogenesis involved in camera-type eye developmentInferred from genetic interaction. Source: MGI oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW positive regulation of apoptosisInferred from genetic interaction. Source: MGI response to drugInferred from direct assay. Source: MGI retinol metabolic processInferred from mutant phenotype. Source: MGI |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 3-chloroallyl aldehyde dehydrogenase activity Inferred from direct assay. Source: MGI retinal dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 501 | 500 | Retinal dehydrogenase 1 | PRO_0000056417 | |||||
Regions | |||||||||
| Nucleotide binding | 246 – 251 | 6 | NAD By similarity | ||||||
| Region | 300 – 305 | 6 | Antabuse binding | ||||||
Sites | |||||||||
| Active site | 269 | 1 | Proton acceptor By similarity | ||||||
| Active site | 303 | 1 | Nucleophile By similarity | ||||||
| Site | 170 | 1 | Transition state stabilizer By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylserine By similarity | ||||||
| Modified residue | 91 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 128 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 252 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 353 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 367 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 410 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 419 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 435 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 495 | 1 | N6-acetyllysine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 8 | 1 | A → R in AAA37202. Ref.1 | ||||||
| Sequence conflict | 8 | 1 | A → R in AAA37203. Ref.1 | ||||||
| Sequence conflict | 45 | 1 | T → S in AAB32754. Ref.2 | ||||||
| Sequence conflict | 51 | 1 | H → Q in AAB32754. Ref.2 | ||||||
| Sequence conflict | 87 | 1 | R → C in AAA37202. Ref.1 | ||||||
| Sequence conflict | 140 | 1 | I → Y AA sequence Ref.4 | ||||||
| Sequence conflict | 458 | 1 | M → I in AAA37202. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of a cytosolic aldehyde dehydrogenase-encoding cDNA from mouse liver." Rongnoparut P., Weaver S. Gene 101:261-265(1991) [PubMed: 2055490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: BALB/c and C57BL/6. Tissue: Liver. |
| [2] | "DNA sequence analysis of the cytosolic acetaldehyde dehydrogenase gene (Ahd-2) in mouse strains with variable ethanol preferences." Bond S.L., Singh S.M. Biochem. Med. Metab. Biol. 52:155-159(1994) [PubMed: 7993664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/ReJ, BALB/c and C57BL/6J. Tissue: Liver. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon and Liver. |
| [4] | "Aldehyde dehydrogenase is a positional marker in the retina." McCaffery P., Tempst P., Lara G., Drager U.C. Development 112:693-702(1991) [PubMed: 1935685] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-52; 140-156; 211-230; 309-320; 330-349; 400-410; 421-435 AND 477-490. Tissue: Embryonic retina. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M74570 mRNA. Translation: AAA37202.1. M74571 Genomic DNA. Translation: AAA37203.1. S75713 mRNA. Translation: AAB32754.2. S77047 Genomic DNA. No translation available. BC044729 mRNA. Translation: AAH44729.1. Different initiation. BC054386 mRNA. Translation: AAH54386.1. | |
| IPI | IPI00626662. |
| PIR | JQ1004. |
| RefSeq | NP_038495.2. |
| UniGene | Mm.435667 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BXS based on UniProtKB P51977. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P24549. |
PTM databases | |
| PhosphoSite | P24549. |
2-D gel databases | |
| SWISS-2DPAGE | P24549. |
| REPRODUCTION-2DPAGE | P24549. |
Proteomic databases | |
| PRIDE | P24549. |
Genome annotation databases | |
| Ensembl | ENSMUST00000087638; ENSMUSP00000084918; ENSMUSG00000053279; Mus musculus. [Genome view] |
| GeneID | 11668. |
| KEGG | mmu:11668. |
| UCSC | uc008gym.1. mouse. |
Organism-specific databases | |
| CTD | 11668. |
| MGI | MGI:1353450. Aldh1a1. |
Phylogenomic databases | |
| HOGENOM | P24549. |
| HOVERGEN | P24549. |
| OMA | VNCYSVV. |
Enzyme and pathway databases | |
| BRENDA | 1.2.1.36. 244. |
Gene expression databases | |
| ArrayExpress | P24549. |
| Bgee | P24549. |
| CleanEx | MM_ALDH1A1. |
| Genevestigator | P24549. |
| GermOnline | ENSMUSG00000053279. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view] |
| Gene3D | G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| PANTHER | PTHR11699. Aldehyde_dehyd. 1 hit. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| PROSITE | PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 279287. |
| SOURCE | Search... |
Entry information
| Entry name | AL1A1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P24549 Secondary accession number(s): Q7TQJ0, Q811J0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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