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Protein

Retinal dehydrogenase 1

Gene

Aldh1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (By similarity).By similarity

Catalytic activityi

Retinal + NAD+ + H2O = retinoate + NADH.By similarity

Pathwayi: retinol metabolism

This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei170Transition state stabilizerBy similarity1
Active sitei269Proton acceptorPROSITE-ProRule annotation1
Active sitei303NucleophilePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 170NADBy similarity4
Nucleotide bindingi193 – 196NADBy similarity4
Nucleotide bindingi226 – 227NADBy similarity2
Nucleotide bindingi246 – 247NADBy similarity2
Nucleotide bindingi269 – 271NADBy similarity3
Nucleotide bindingi349 – 353NADBy similarity5
Nucleotide bindingi400 – 402NADBy similarity3

GO - Molecular functioni

GO - Biological processi

  • 9-cis-retinoic acid biosynthetic process Source: MGI
  • embryonic eye morphogenesis Source: MGI
  • optic cup morphogenesis involved in camera-type eye development Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • response to drug Source: MGI
  • retinoic acid metabolic process Source: MGI
  • retinol metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.36. 3474.
ReactomeiR-MMU-5365859. RA biosynthesis pathway.
R-MMU-70350. Fructose catabolism.
R-MMU-71384. Ethanol oxidation.
SABIO-RKP24549.
UniPathwayiUPA00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinal dehydrogenase 1Curated (EC:1.2.1.-By similarity, EC:1.2.1.36By similarity)
Short name:
RALDH 1Curated
Short name:
RalDH1Curated
Alternative name(s):
ALDH-E1
ALHDII
Aldehyde dehydrogenase family 1 member A1Imported
Aldehyde dehydrogenase, cytosolic1 Publication
Gene namesi
Name:Aldh1a1Imported
Synonyms:Ahd-2, Ahd2, Aldh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1353450. Aldh1a1.

Subcellular locationi

  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000564172 – 501Retinal dehydrogenase 1Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei128N6-acetyllysineBy similarity1
Modified residuei252N6-acetyllysineBy similarity1
Modified residuei337PhosphothreonineBy similarity1
Modified residuei353N6-acetyllysineBy similarity1
Modified residuei367N6-acetyllysineBy similarity1
Modified residuei410N6-acetyllysineBy similarity1
Modified residuei413PhosphoserineBy similarity1
Modified residuei419N6-acetyllysineBy similarity1
Modified residuei435N6-acetyllysineBy similarity1
Modified residuei495N6-acetyllysineBy similarity1

Post-translational modificationi

The N-terminus is blocked most probably by acetylation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP24549.
PaxDbiP24549.
PeptideAtlasiP24549.
PRIDEiP24549.

2D gel databases

REPRODUCTION-2DPAGEIPI00626662.
P24549.
SWISS-2DPAGEP24549.

PTM databases

iPTMnetiP24549.
PhosphoSitePlusiP24549.
SwissPalmiP24549.

Expressioni

Tissue specificityi

Expressed in the liver, lung, and testis. Apparently not expressed at detectable levels in kidney, stomach, ovary, heart, and brain.

Gene expression databases

BgeeiENSMUSG00000053279.
CleanExiMM_ALDH1A1.
GenevisibleiP24549. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP24549. 4 interactors.
MINTiMINT-1859401.
STRINGi10090.ENSMUSP00000084918.

Structurei

3D structure databases

ProteinModelPortaliP24549.
SMRiP24549.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP24549.
KOiK07249.
OMAiSIAGTIC.
OrthoDBiEOG091G05E8.
PhylomeDBiP24549.
TreeFamiTF300455.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPAQPAVP APLADLKIQH TKIFINNEWH NSVSGKKFPV LNPATEEVIC
60 70 80 90 100
HVEEGDKADV DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLMERDR
110 120 130 140 150
LLLATMEALN GGKVFANAYL SDLGGCIKAL KYCAGWADKI HGQTIPSDGD
160 170 180 190 200
IFTYTRREPI GVCGQIIPWN FPMLMFIWKI GPALSCGNTV VVKPAEQTPL
210 220 230 240 250
TALHLASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD KVAFTGSTQV
260 270 280 290 300
GKLIKEAAGK SNLKRVTLEL GGKSPCIVFA DADLDIAVEF AHHGVFYHQG
310 320 330 340 350
QCCVAASRIF VEESVYDEFV KRSVERAKKY VLGNPLTPGI NQGPQIDKEQ
360 370 380 390 400
HDKILDLIES GKKEGAKLEC GGGRWGNKGF FVQPTVFSNV TDEMRIAKEE
410 420 430 440 450
IFGPVQQIMK FKSVDDVIKR ANNTTYGLAA GLFTKDLDKA ITVSSALQAG
460 470 480 490 500
VVWVNCYMML SAQCPFGGFK MSGNGRELGE HGLYEYTELK TVAMKISQKN

S
Length:501
Mass (Da):54,468
Last modified:January 23, 2007 - v5
Checksum:i0E428151B799BD1D
GO

Sequence cautioni

The sequence AAH44729 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8A → R in AAA37202 (PubMed:2055490).Curated1
Sequence conflicti8A → R in AAA37203 (PubMed:2055490).Curated1
Sequence conflicti45T → S in AAB32754 (PubMed:7993664).Curated1
Sequence conflicti51H → Q in AAB32754 (PubMed:7993664).Curated1
Sequence conflicti87R → C in AAA37202 (PubMed:2055490).Curated1
Sequence conflicti140I → Y AA sequence (PubMed:1935685).Curated1
Sequence conflicti458M → I in AAA37202 (PubMed:2055490).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74570 mRNA. Translation: AAA37202.1.
M74571 Genomic DNA. Translation: AAA37203.1.
S75713 mRNA. Translation: AAB32754.2.
S77047 Genomic DNA. No translation available.
BC044729 mRNA. Translation: AAH44729.1. Different initiation.
BC054386 mRNA. Translation: AAH54386.1.
CCDSiCCDS29695.1.
PIRiJQ1004.
RefSeqiNP_038495.2. NM_013467.3.
UniGeneiMm.250866.

Genome annotation databases

EnsembliENSMUST00000087638; ENSMUSP00000084918; ENSMUSG00000053279.
GeneIDi11668.
KEGGimmu:11668.
UCSCiuc008gym.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74570 mRNA. Translation: AAA37202.1.
M74571 Genomic DNA. Translation: AAA37203.1.
S75713 mRNA. Translation: AAB32754.2.
S77047 Genomic DNA. No translation available.
BC044729 mRNA. Translation: AAH44729.1. Different initiation.
BC054386 mRNA. Translation: AAH54386.1.
CCDSiCCDS29695.1.
PIRiJQ1004.
RefSeqiNP_038495.2. NM_013467.3.
UniGeneiMm.250866.

3D structure databases

ProteinModelPortaliP24549.
SMRiP24549.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24549. 4 interactors.
MINTiMINT-1859401.
STRINGi10090.ENSMUSP00000084918.

PTM databases

iPTMnetiP24549.
PhosphoSitePlusiP24549.
SwissPalmiP24549.

2D gel databases

REPRODUCTION-2DPAGEIPI00626662.
P24549.
SWISS-2DPAGEP24549.

Proteomic databases

MaxQBiP24549.
PaxDbiP24549.
PeptideAtlasiP24549.
PRIDEiP24549.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087638; ENSMUSP00000084918; ENSMUSG00000053279.
GeneIDi11668.
KEGGimmu:11668.
UCSCiuc008gym.1. mouse.

Organism-specific databases

CTDi216.
MGIiMGI:1353450. Aldh1a1.

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP24549.
KOiK07249.
OMAiSIAGTIC.
OrthoDBiEOG091G05E8.
PhylomeDBiP24549.
TreeFamiTF300455.

Enzyme and pathway databases

UniPathwayiUPA00912.
BRENDAi1.2.1.36. 3474.
ReactomeiR-MMU-5365859. RA biosynthesis pathway.
R-MMU-70350. Fructose catabolism.
R-MMU-71384. Ethanol oxidation.
SABIO-RKP24549.

Miscellaneous databases

PROiP24549.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053279.
CleanExiMM_ALDH1A1.
GenevisibleiP24549. MM.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAL1A1_MOUSE
AccessioniPrimary (citable) accession number: P24549
Secondary accession number(s): Q7TQJ0, Q811J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 163 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.