Reviewed, UniProtKB/Swiss-Prot P24548 (PGLR_OENOR)

Names and origin

Protein names	Recommended name:     Exopolygalacturonase       Short name=ExoPG     EC=3.2.1.67 Alternative name(s):     Pectinase     Galacturan 1,4-alpha-galacturonidase
Organism	Oenothera organensis (Evening primrose)
Taxonomic identifier	3945 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Myrtales › Onagraceae › Oenothera

Protein attributes

Sequence length	362 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.
Catalytic activity	((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.
Subcellular location	Secreted By similarity. Secreted › cell wall By similarity.
Tissue specificity	Pollen tubes growing through the style during pollination.
Developmental stage	Accumulates late in pollen development and/or during germination and tube growth.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 4 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galacturan 1,4-alpha-galacturonidase activity Inferred from electronic annotation. Source: EC polygalacturonase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 362	›362	Exopolygalacturonase		PRO_0000215229
Regions
	Repeat	138 – 164	27	PbH1 1
	Repeat	165 – 186	22	PbH1 2
	Repeat	188 – 208	21	PbH1 3
	Repeat	218 – 239	22	PbH1 4
Sites
	Active site	179	1	Proton donor By similarity
	Active site	202	1	By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P24548-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 0AD6F45B5518B947	FASTA	362	38,207
	10 20 30 40 50 60 DSTQALTTAW KEACASASPS TILVPKGNFA VGLITLEGPC KSSIGLQLQG TLKAPADPSK 70 80 90 100 110 120 IKGLGWINLN KIDLLTIFGG GVFDGQGKSA WVQNDCHKNG PICKTLSMNL RLYAVTNSIL 130 140 150 160 170 180 RDVTTLDSKN FHVNVIGCKN LTFERFKISA AETSINTDGI HIGRSDGVNI INTEIKTGDD 190 200 210 220 230 240 CISLGDGSKN INITNITCGP GHGISVGSLG RYKNEESVVG IYVKNCTITG SQNGVRIKTW 250 260 270 280 290 300 PKSEPGEASE MHFQDITMNS VGTPILIDQG YCPYNQCTAE VPSSVKLSKI SFKNIKGTST 310 320 330 340 350 360 TKEAVKLVCS KSFPCNGVEL ADIDLTYSGK GGPATSVCEN IKPTIKGKQI PAICSGSAAK AA

References

[1]	"Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonase." Brown S.M., Crouch M.L. Plant Cell 2:263-274(1990) [PubMed: 2152116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Pollen.

Cross-references

Sequence databases
PIR	JQ0992.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.67. 290412.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_OENOR

Accession

Primary (citable) accession number: P24548

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)