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P24547 (IMDH2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inosine-5'-monophosphate dehydrogenase 2

Short name=IMP dehydrogenase 2
Short name=IMPD 2
Short name=IMPDH 2
EC=1.1.1.205
Alternative name(s):
IMPDH-II
Gene names
Name:Impdh2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism By similarity. It may also have a role in the development of malignancy and the growth progression of some tumors. HAMAP-Rule MF_03156

Catalytic activity

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH. HAMAP-Rule MF_03156

Cofactor

Potassium By similarity. HAMAP-Rule MF_03156

Enzyme regulation

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity. HAMAP-Rule MF_03156

Pathway

Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. HAMAP-Rule MF_03156

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity HAMAP-Rule MF_03156.

Sequence similarities

Belongs to the IMPDH/GMPR family.

Contains 2 CBS domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 514513Inosine-5'-monophosphate dehydrogenase 2 HAMAP-Rule MF_03156
PRO_0000093674

Regions

Domain114 – 17360CBS 1
Domain179 – 23759CBS 2
Nucleotide binding274 – 2763NAD By similarity
Nucleotide binding324 – 3263NAD By similarity
Region364 – 3663IMP binding By similarity
Region387 – 3882IMP binding By similarity
Region411 – 4155IMP binding By similarity

Sites

Active site3311Thioimidate intermediate By similarity
Metal binding3261Potassium; via carbonyl oxygen By similarity
Metal binding3281Potassium; via carbonyl oxygen By similarity
Metal binding3311Potassium; via carbonyl oxygen By similarity
Metal binding5001Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal binding5011Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal binding5021Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Binding site3291IMP By similarity
Binding site4411IMP By similarity

Amino acid modifications

Modified residue1221Phosphoserine By similarity
Modified residue4001Phosphotyrosine By similarity
Modified residue4161Phosphoserine By similarity
Modified residue5111N6-acetyllysine By similarity

Natural variations

Natural variant3331T → I in mycophenolic acid resistant cells.
Natural variant3511S → Y in mycophenolic acid resistant cells.

Experimental info

Sequence conflict4581P → L AA sequence Ref.4
Sequence conflict4651Q → S AA sequence Ref.4
Sequence conflict4831M → T in AAA39311. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24547 [UniParc].

Last modified October 3, 2003. Version 2.
Checksum: 17D25A5C5EBCC439

FASTA51455,815
        10         20         30         40         50         60 
MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD LTSALTKKIT 

        70         80         90        100        110        120 
LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ ANEVRKVKKY EQGFITDPVV 

       130        140        150        160        170        180 
LSPKDRVRDV FEAKARHGFC GIPITDTGRM GSRLVGIISS RDIDFLKEEE HDRFLEEIMT 

       190        200        210        220        230        240 
KREDLVVAPA GVTLKEANEI LQRSKKGKLP IVNENDELVA IIARTDLKKN RDYPLASKDA 

       250        260        270        280        290        300 
KKQLLCGAAI GTHEDDKYRL DLLALAGVDV VVLDSSQGNS IFQINMIKYI KEKYPSLQVI 

       310        320        330        340        350        360 
GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI CITQEVLACG RPQATAVYKV SEYARRFGVP 

       370        380        390        400        410        420 
VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY FFSDGIRLKK YRGMGSLDAM 

       430        440        450        460        470        480 
DKHLSSQNRY FSEADKIKVA QGVSGAVQDK GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR 

       490        500        510 
AMMYSGELKF EKRTSSAQVE GGVHSLHSYE KRLF 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and sequence of a cDNA encoding mouse IMP dehydrogenase."
Tiedeman A.A., Smith J.M.
Gene 97:289-293(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Gene amplification and dual point mutations of mouse IMP dehydrogenase associated with cellular resistance to mycophenolic acid."
Lightfoot T., Snyder F.F.
Biochim. Biophys. Acta 1217:156-162(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MYCOPHENOLIC ACID RESISTANT.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C3H/He and Czech II.
Tissue: Mammary gland and Osteoblast.
[4]"Increased activity, amount, and altered kinetic properties of IMP dehydrogenase from mycophenolic acid-resistant neuroblastoma cells."
Hodges S.D., Fung E., McKay D.J., Renaux B.S., Snyder F.F.
J. Biol. Chem. 264:18137-18141(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 125-133; 182-194; 289-290; 439-449; 456-466 AND 475-478.
[5]Lubec G., Yang J.W., Zigmond M.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 137-149.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33934 mRNA. Translation: AAA39311.1.
M98333 mRNA. Translation: AAA20181.1.
BC010314 mRNA. Translation: AAH10314.1.
BC052671 mRNA. Translation: AAH52671.1.
CCDSCCDS40768.1.
PIRJT0565.
RefSeqNP_035960.2. NM_011830.3.
UniGeneMm.6065.

3D structure databases

ProteinModelPortalP24547.
SMRP24547. Positions 10-514.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204792. 2 interactions.
IntActP24547. 2 interactions.
MINTMINT-1869864.

Chemistry

BindingDBP24547.
ChEMBLCHEMBL3169.

PTM databases

PhosphoSiteP24547.

2D gel databases

REPRODUCTION-2DPAGEP24547.

Proteomic databases

MaxQBP24547.
PaxDbP24547.
PRIDEP24547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081111; ENSMUSP00000079888; ENSMUSG00000062867.
GeneID23918.
KEGGmmu:23918.
UCSCuc009rqg.1. mouse.

Organism-specific databases

CTD3615.
MGIMGI:109367. Impdh2.

Phylogenomic databases

eggNOGCOG0517.
HOGENOMHOG000165752.
HOVERGENHBG052122.
InParanoidP24547.
KOK00088.
OMAHAAKDEH.
OrthoDBEOG7VTDMM.
PhylomeDBP24547.
TreeFamTF300378.

Enzyme and pathway databases

UniPathwayUPA00601; UER00295.

Gene expression databases

BgeeP24547.
CleanExMM_IMPDH2.
GenevestigatorP24547.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01964. IMPDH.
InterProIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFPIRSF000130. IMPDH. 1 hit.
SMARTSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsTIGR01302. IMP_dehydrog. 1 hit.
PROSITEPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIMPDH2. mouse.
NextBio303689.
PROP24547.
SOURCESearch...

Entry information

Entry nameIMDH2_MOUSE
AccessionPrimary (citable) accession number: P24547
Secondary accession number(s): Q61734, Q91Z11
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 3, 2003
Last modified: July 9, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot