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Protein

Type-2 restriction enzyme AccI

Gene

accIR

Organism
Acinetobacter calcoaceticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GTMKAC and cleaves after T-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi18. AccI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AccI (EC:3.1.21.4)
Short name:
R.AccI
Alternative name(s):
Endonuclease AccI
Type II restriction enzyme AccI
Gene namesi
Name:accIR
OrganismiAcinetobacter calcoaceticus
Taxonomic identifieri471 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Type-2 restriction enzyme AccIPRO_0000077276Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Family & Domainsi

Family and domain databases

InterProiIPR019054. Restrct_endonuc_II_AccI.
[Graphical view]
PfamiPF09545. RE_AccI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24546-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYYDRIREL TKNVPVELVD FEQPRDLART PTQASSNFIT NKEQGDWAED
60 70 80 90 100
LVTRAINENS KNFVAVKYGK SDNLVAGENG FDTFYQDFQT ELDTIGKRPD
110 120 130 140 150
LLIFKKTDFD TTLGFDVSQI PHHQITDYVK KAIAGIEVRS SAFLIDKYEE
160 170 180 190 200
AMQVRTQRFT EIAFQTRDKI LAEFLDVLDH PSRSKYITLL NTLTLETISI
210 220 230 240 250
FDFKVPGWRS NERLIEVNNL FKRLKVAIKE IQKRDYLSIT PKVEDIKVVY
260 270 280 290 300
KWIETFNVPH FYFQVFFDKV YGISFEQILT IISNSDNDGV IFSVEKDVQN
310 320 330 340 350
QNKTTIKINS KTGYPIASKV DEPTHESIRK EMDRGRLLFY VTFKGGTAYL
360
DLDNLRTILG IEEAEF
Length:366
Mass (Da):42,495
Last modified:March 1, 1992 - v1
Checksum:iDB663B74351C415E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10671 Genomic DNA. Translation: BAA01522.1.
PIRiJU0469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10671 Genomic DNA. Translation: BAA01522.1.
PIRiJU0469.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi18. AccI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019054. Restrct_endonuc_II_AccI.
[Graphical view]
PfamiPF09545. RE_AccI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and nucleotide sequences of the AccI restriction-modification genes in Acinetobacter calcoaceticus."
    Kawakami B., Hilzheber C., Nagatomo M., Oka M.
    Agric. Biol. Chem. 55:1553-1559(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: ATCC 49823.

Entry informationi

Entry nameiT2A1_ACICA
AccessioniPrimary (citable) accession number: P24546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.