Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24546 (T2A1_ACICA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme AccI

Short name=R.AccI
EC=3.1.21.4
Alternative name(s):
Endonuclease AccI
Type II restriction enzyme AccI
Gene names
Name:accIR
OrganismAcinetobacter calcoaceticus
Taxonomic identifier471 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GTMKAC and cleaves after T-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

Homotetramer.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Type-2 restriction enzyme AccI
PRO_0000077276

Sequences

Sequence LengthMass (Da)Tools
P24546 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: DB663B74351C415E

FASTA36642,495
        10         20         30         40         50         60 
MDYYDRIREL TKNVPVELVD FEQPRDLART PTQASSNFIT NKEQGDWAED LVTRAINENS 

        70         80         90        100        110        120 
KNFVAVKYGK SDNLVAGENG FDTFYQDFQT ELDTIGKRPD LLIFKKTDFD TTLGFDVSQI 

       130        140        150        160        170        180 
PHHQITDYVK KAIAGIEVRS SAFLIDKYEE AMQVRTQRFT EIAFQTRDKI LAEFLDVLDH 

       190        200        210        220        230        240 
PSRSKYITLL NTLTLETISI FDFKVPGWRS NERLIEVNNL FKRLKVAIKE IQKRDYLSIT 

       250        260        270        280        290        300 
PKVEDIKVVY KWIETFNVPH FYFQVFFDKV YGISFEQILT IISNSDNDGV IFSVEKDVQN 

       310        320        330        340        350        360 
QNKTTIKINS KTGYPIASKV DEPTHESIRK EMDRGRLLFY VTFKGGTAYL DLDNLRTILG 


IEEAEF 

« Hide

References

[1]"Cloning and nucleotide sequences of the AccI restriction-modification genes in Acinetobacter calcoaceticus."
Kawakami B., Hilzheber C., Nagatomo M., Oka M.
Agric. Biol. Chem. 55:1553-1559(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
Strain: ATCC 49823.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10671 Genomic DNA. Translation: BAA01522.1.
PIRJU0469.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE18. AccI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019054. Restrct_endonuc_II_AccI.
[Graphical view]
PfamPF09545. RE_AccI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2A1_ACICA
AccessionPrimary (citable) accession number: P24546
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries