Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24546

- T2A1_ACICA

UniProt

P24546 - T2A1_ACICA

Protein

Type-2 restriction enzyme AccI

Gene

accIR

Organism
Acinetobacter calcoaceticus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 55 (16 Apr 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Recognizes the double-stranded sequence GTMKAC and cleaves after T-2.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi18. AccI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme AccI (EC:3.1.21.4)
    Short name:
    R.AccI
    Alternative name(s):
    Endonuclease AccI
    Type II restriction enzyme AccI
    Gene namesi
    Name:accIR
    OrganismiAcinetobacter calcoaceticus
    Taxonomic identifieri471 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366Type-2 restriction enzyme AccIPRO_0000077276Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Family & Domainsi

    Family and domain databases

    InterProiIPR019054. Restrct_endonuc_II_AccI.
    [Graphical view]
    PfamiPF09545. RE_AccI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24546-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYYDRIREL TKNVPVELVD FEQPRDLART PTQASSNFIT NKEQGDWAED    50
    LVTRAINENS KNFVAVKYGK SDNLVAGENG FDTFYQDFQT ELDTIGKRPD 100
    LLIFKKTDFD TTLGFDVSQI PHHQITDYVK KAIAGIEVRS SAFLIDKYEE 150
    AMQVRTQRFT EIAFQTRDKI LAEFLDVLDH PSRSKYITLL NTLTLETISI 200
    FDFKVPGWRS NERLIEVNNL FKRLKVAIKE IQKRDYLSIT PKVEDIKVVY 250
    KWIETFNVPH FYFQVFFDKV YGISFEQILT IISNSDNDGV IFSVEKDVQN 300
    QNKTTIKINS KTGYPIASKV DEPTHESIRK EMDRGRLLFY VTFKGGTAYL 350
    DLDNLRTILG IEEAEF 366
    Length:366
    Mass (Da):42,495
    Last modified:March 1, 1992 - v1
    Checksum:iDB663B74351C415E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10671 Genomic DNA. Translation: BAA01522.1.
    PIRiJU0469.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10671 Genomic DNA. Translation: BAA01522.1 .
    PIRi JU0469.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 18. AccI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019054. Restrct_endonuc_II_AccI.
    [Graphical view ]
    Pfami PF09545. RE_AccI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequences of the AccI restriction-modification genes in Acinetobacter calcoaceticus."
      Kawakami B., Hilzheber C., Nagatomo M., Oka M.
      Agric. Biol. Chem. 55:1553-1559(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
      Strain: ATCC 49823.

    Entry informationi

    Entry nameiT2A1_ACICA
    AccessioniPrimary (citable) accession number: P24546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: April 16, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3