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Protein

Argininosuccinate synthase

Gene

argG

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei99 – 991CitrullineBy similarity
Binding sitei129 – 1291ATP; via amide nitrogenBy similarity
Binding sitei131 – 1311AspartateBy similarity
Binding sitei131 – 1311ATPBy similarity
Binding sitei135 – 1351AspartateBy similarity
Binding sitei135 – 1351CitrullineBy similarity
Binding sitei136 – 1361AspartateBy similarity
Binding sitei136 – 1361ATPBy similarity
Binding sitei139 – 1391CitrullineBy similarity
Binding sitei192 – 1921CitrullineBy similarity
Binding sitei194 – 1941ATPBy similarity
Binding sitei201 – 2011CitrullineBy similarity
Binding sitei203 – 2031CitrullineBy similarity
Binding sitei280 – 2801CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:SCO7036
ORF Names:SC4G1.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 481480Argininosuccinate synthasePRO_0000148708Add
BLAST

Proteomic databases

PRIDEiP24532.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi100226.SCO7036.

Structurei

3D structure databases

ProteinModelPortaliP24532.
SMRiP24532. Positions 2-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
InParanoidiP24532.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.
PhylomeDBiP24532.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVLTSLPT GERVGIAFSG GLDTSVAVAW MRDKGAVPCT YTADIGQYDE
60 70 80 90 100
PDIASVPDRA KTYGAEVARL VDCRAALVEE GLAALTCGAF HIRSGGRAYF
110 120 130 140 150
NTTPLGRAVT GTLLVRAMLE DDVQIWGDGS TFKGNDIERF YRYGLLANPQ
160 170 180 190 200
LRIYKPWLDA DFVTELGGRK EMSEWLVAHD LPYRDSTEKA YSTDANIWGA
210 220 230 240 250
THEAKTLEHL DTGVETVEPI MGVRFWDPSV EIAPEDVTIG FDQGRPVTIN
260 270 280 290 300
GKEFASAVDL VMEANAVGGR HGMGMSDQIE NRIIEAKSRG IYEAPGMALL
310 320 330 340 350
HAAYERLVNA IHNEDTLAQY HTEGRRLGRL MYEGRWLDPQ SLMIRESLQR
360 370 380 390 400
WVGSAVTGEV TLRLRRGEDY SILDTTGPAF SYHPDKLSME RTEDSAFGPV
410 420 430 440 450
DRIGQLTMRN LDIADSRAKL EQYAGLGLIG TANPAIGAAQ AAATGLIGAM
460 470 480
PEGGAQAIAS RGEVSADDEL LDRAAMESGT D
Length:481
Mass (Da):52,270
Last modified:January 23, 2007 - v4
Checksum:i18E7DE4BEABF98B3
GO

Sequence cautioni

The sequence BAA00691.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 839AALVEEGLA → RRWSRRGWG in BAA00691 (Ref. 1) Curated
Sequence conflicti181 – 1811L → F in BAA00691 (Ref. 1) Curated
Sequence conflicti265 – 2728NAVGGRHG → TPSAAGTA in BAA00691 (Ref. 1) Curated
Sequence conflicti326 – 3261R → P in BAA00691 (Ref. 1) Curated
Sequence conflicti426 – 4283LGL → SRF in BAA00691 (Ref. 1) Curated
Sequence conflicti435 – 4351A → P in BAA00691 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00799 Genomic DNA. Translation: BAA00691.1. Different initiation.
AL939130 Genomic DNA. Translation: CAC01534.1.
PIRiJQ1057. AJSMRC.
RefSeqiNP_631098.1. NC_003888.3.
WP_003972108.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAC01534; CAC01534; CAC01534.
GeneIDi1102474.
KEGGisco:SCO7036.
PATRICi23743995. VBIStrCoe124346_7140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00799 Genomic DNA. Translation: BAA00691.1. Different initiation.
AL939130 Genomic DNA. Translation: CAC01534.1.
PIRiJQ1057. AJSMRC.
RefSeqiNP_631098.1. NC_003888.3.
WP_003972108.1. NC_003888.3.

3D structure databases

ProteinModelPortaliP24532.
SMRiP24532. Positions 2-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO7036.

Proteomic databases

PRIDEiP24532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC01534; CAC01534; CAC01534.
GeneIDi1102474.
KEGGisco:SCO7036.
PATRICi23743995. VBIStrCoe124346_7140.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
InParanoidiP24532.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.
PhylomeDBiP24532.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the gene encoding argininosuccinate synthetase in Streptomyces coelicolor A3(2)."
    Ishihara H., Urabe H., Kasama H., Ogawara H.
    Nihon Hosenkin Gakkaishi 5:14-17(1991)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A3(2) / NRRL B-16638.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiASSY_STRCO
AccessioniPrimary (citable) accession number: P24532
Secondary accession number(s): Q9FC47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 123 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.