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P24531 (GLYA_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:Cj0402
OrganismCampylobacter jejuni
Taxonomic identifier197 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_0000113554

Regions

Region120 – 1223Substrate binding By similarity

Sites

Binding site301Pyridoxal phosphate By similarity
Binding site501Pyridoxal phosphate By similarity
Binding site521Substrate By similarity
Binding site591Substrate binding By similarity
Binding site601Pyridoxal phosphate By similarity
Binding site941Pyridoxal phosphate By similarity
Binding site1161Substrate By similarity
Binding site1701Pyridoxal phosphate By similarity
Binding site1981Pyridoxal phosphate By similarity
Binding site2231Pyridoxal phosphate By similarity
Binding site2301Pyridoxal phosphate By similarity
Binding site2561Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3561Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2241N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict1881I → V in CAA37812. Ref.1
Sequence conflict2141Y → H in CAA37812. Ref.1
Sequence conflict3861V → I in CAA37812. Ref.1

Secondary structure

1 ............................................................. 414
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24531 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 91225A45CD9193E6

FASTA41445,778
        10         20         30         40         50         60 
MSLEMFDKEI FDLTNKELER QCEGLEMIAS ENFTLPEVME VMGSILTNKY AEGYPGKRYY 

        70         80         90        100        110        120 
GGCEFVDEIE TLAIERCKKL FNCKFANVQP NSGSQANQGV YAALINPGDK ILGMDLSHGG 

       130        140        150        160        170        180 
HLTHGAKVSS SGKMYESCFY GVELDGRIDY EKVREIAKKE KPKLIVCGAS AYARVIDFAK 

       190        200        210        220        230        240 
FREIADEIGA YLFADIAHIA GLVVAGEHPS PFPYAHVVSS TTHKTLRGPR GGIIMTNDEE 

       250        260        270        280        290        300 
LAKKINSAIF PGIQGGPLMH VIAAKAVGFK FNLSDEWKVY AKQVRTNAQV LANVLMDRKF 

       310        320        330        340        350        360 
KLVSDGTDNH LVLMSFLDRE FSGKDADLAL GNAGITANKN TVPGEIRSPF ITSGLRLGTP 

       370        380        390        400        410 
ALTARGFKEK EMEIVSNYIA DILDDVNNEK LQENIKQELK KLASNFIIYE RAMF

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of the Campylobacter jejuni glyA gene encoding serine hydroxymethyltransferase."
Chan V.L., Bingham H.L.
Gene 101:51-58(1991) [PubMed: 2060796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences."
Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. expand/collapse author list , van Vliet A.H.M., Whitehead S., Barrell B.G.
Nature 403:665-668(2000) [PubMed: 10688204] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 11168 / Serotype O:2.
[3]"Crystal structure of serine hydroxymethyltransferase from Campylobacter jejuni."
Center for structural genomics of infectious diseases (CSGID)
Anderson S.M., Wawrzak Z., Onopriyenko O., Hasseman J., Anderson W.F., Savchenko A.
Submitted (MAY-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS), SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X53816 Genomic DNA. Translation: CAA37812.1.
AL111168 Genomic DNA. Translation: CAL34552.1.
PIRH81383.
JQ1016.
RefSeqYP_002343839.1. NC_002163.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3N0LX-ray1.80A/B1-414[»]
ProteinModelPortalP24531.
SMRP24531. Positions 2-403.
ModBaseSearch...

Protein-protein interaction databases

IntActP24531. 45 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID904726.
GenomeReviewsGene locus Cj0402 in contig AL111168_GR.
KEGGcje:Cj0402.
PATRIC20057719. VBICamJej33762_0393.

Phylogenomic databases

HOGENOMHBG301263.
OMAHCHVATT.
ProtClustDBPRK13034.

Enzyme and pathway databases

BioCycCJEJ192222:CJ0402-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_CAMJE
AccessionPrimary (citable) accession number: P24531
Secondary accession number(s): Q0PBA8, Q9PIA3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 1, 2000
Last modified: January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents