Reviewed, UniProtKB/Swiss-Prot P24531 (GLYA_CAMJE)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase
      Short name=Serine methylase
      Short name=SHMT
    EC=2.1.2.1

Gene names

Name:	glyA
Ordered Locus Names:	Cj0402

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00051
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flaG	Q0PAW9	1	EBI-1327127,EBI-1191365

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Serine hydroxymethyltransferase HAMAP MF_00051		PRO_0000113554
Amino acid modifications
	Modified residue	224	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	188	1	I → V in CAA37812. Ref.1
	Sequence conflict	214	1	Y → H in CAA37812. Ref.1
	Sequence conflict	386	1	V → I in CAA37812. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24531-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 91225A45CD9193E6	FASTA	414	45,778
	10 20 30 40 50 60 MSLEMFDKEI FDLTNKELER QCEGLEMIAS ENFTLPEVME VMGSILTNKY AEGYPGKRYY 70 80 90 100 110 120 GGCEFVDEIE TLAIERCKKL FNCKFANVQP NSGSQANQGV YAALINPGDK ILGMDLSHGG 130 140 150 160 170 180 HLTHGAKVSS SGKMYESCFY GVELDGRIDY EKVREIAKKE KPKLIVCGAS AYARVIDFAK 190 200 210 220 230 240 FREIADEIGA YLFADIAHIA GLVVAGEHPS PFPYAHVVSS TTHKTLRGPR GGIIMTNDEE 250 260 270 280 290 300 LAKKINSAIF PGIQGGPLMH VIAAKAVGFK FNLSDEWKVY AKQVRTNAQV LANVLMDRKF 310 320 330 340 350 360 KLVSDGTDNH LVLMSFLDRE FSGKDADLAL GNAGITANKN TVPGEIRSPF ITSGLRLGTP 370 380 390 400 410 ALTARGFKEK EMEIVSNYIA DILDDVNNEK LQENIKQELK KLASNFIIYE RAMF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of the Campylobacter jejuni glyA gene encoding serine hydroxymethyltransferase." Chan V.L., Bingham H.L. Gene 101:51-58(1991) [PubMed: 2060796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53816 Genomic DNA. Translation: CAA37812.1. AL111168 Genomic DNA. Translation: CAL34552.1.
PIR	H81383. JQ1016.
RefSeq	YP_002343839.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFO based on UniProtKB P00477.
ModBase	Search...
Protein-protein interaction databases
IntAct	P24531. 45 interactions.
Genome annotation databases
GeneID	904726.
GenomeReviews	Gene locus Cj0402 in contig AL111168_GR.
KEGG	cje:Cj0402.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P24531.
OMA	P24531. AYSLRID.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0402-MON.
BRENDA	2.1.2.1. 255835.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_CAMJE

Accession

Primary (citable) accession number: P24531
Secondary accession number(s): Q0PBA8, Q9PIA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)