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Protein

Tyrosine 3-monooxygenase

Gene

Th

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the physiology of adrenergic neurons.

Catalytic activityi

L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactori

Enzyme regulationi

Phosphorylation leads to an increase in the catalytic activity.

Pathway: dopamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dopamine from L-tyrosine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tyrosine 3-monooxygenase (Th)
  2. Aromatic-L-amino-acid decarboxylase (Ddc)
This subpathway is part of the pathway dopamine biosynthesis, which is itself part of Catecholamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dopamine from L-tyrosine, the pathway dopamine biosynthesis and in Catecholamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi331 – 3311IronBy similarity
Metal bindingi336 – 3361IronBy similarity
Metal bindingi376 – 3761IronBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to drug Source: Ensembl
  • cellular response to glucose stimulus Source: Ensembl
  • cellular response to growth factor stimulus Source: Ensembl
  • cellular response to manganese ion Source: Ensembl
  • cellular response to nicotine Source: Ensembl
  • cerebral cortex development Source: Ensembl
  • circadian sleep/wake cycle Source: Ensembl
  • dopamine biosynthetic process Source: MGI
  • dopamine biosynthetic process from tyrosine Source: MGI
  • eating behavior Source: MGI
  • embryonic camera-type eye morphogenesis Source: BHF-UCL
  • epinephrine biosynthetic process Source: MGI
  • eye photoreceptor cell development Source: BHF-UCL
  • fatty acid metabolic process Source: Ensembl
  • glycoside metabolic process Source: Ensembl
  • heart development Source: MGI
  • isoquinoline alkaloid metabolic process Source: Ensembl
  • learning Source: MGI
  • locomotory behavior Source: MGI
  • mating behavior Source: MGI
  • memory Source: MGI
  • multicellular organismal aging Source: Ensembl
  • neurotransmitter biosynthetic process Source: UniProtKB-KW
  • norepinephrine biosynthetic process Source: MGI
  • organ morphogenesis Source: MGI
  • phthalate metabolic process Source: Ensembl
  • phytoalexin metabolic process Source: Ensembl
  • regulation of heart contraction Source: MGI
  • response to activity Source: Ensembl
  • response to amphetamine Source: Ensembl
  • response to corticosterone Source: Ensembl
  • response to electrical stimulus Source: Ensembl
  • response to estradiol Source: Ensembl
  • response to ethanol Source: MGI
  • response to ether Source: Ensembl
  • response to herbicide Source: Ensembl
  • response to hypoxia Source: MGI
  • response to immobilization stress Source: Ensembl
  • response to light stimulus Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to nutrient levels Source: Ensembl
  • response to peptide hormone Source: Ensembl
  • response to pyrethroid Source: Ensembl
  • response to salt stress Source: Ensembl
  • response to water deprivation Source: Ensembl
  • response to zinc ion Source: Ensembl
  • sensory perception of sound Source: Ensembl
  • social behavior Source: Ensembl
  • sphingolipid metabolic process Source: Ensembl
  • synaptic transmission, dopaminergic Source: MGI
  • synaptic vesicle amine transport Source: Ensembl
  • terpene metabolic process Source: Ensembl
  • visual perception Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Catecholamine biosynthesis, Neurotransmitter biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_295408. Catecholamine biosynthesis.
UniPathwayiUPA00747; UER00733.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine 3-monooxygenase (EC:1.14.16.2)
Alternative name(s):
Tyrosine 3-hydroxylase
Short name:
TH
Gene namesi
Name:Th
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98735. Th.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic side of plasma membrane Source: MGI
  • cytoplasmic vesicle Source: MGI
  • cytosol Source: Ensembl
  • dendrite Source: Ensembl
  • melanosome membrane Source: MGI
  • mitochondrion Source: Ensembl
  • neuron projection Source: MGI
  • nucleus Source: MGI
  • perikaryon Source: MGI
  • smooth endoplasmic reticulum Source: MGI
  • synaptic vesicle Source: Ensembl
  • terminal bouton Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 498497Tyrosine 3-monooxygenasePRO_0000205562Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine; by CaMK2By similarity
Modified residuei31 – 311PhosphoserineBy similarity
Modified residuei40 – 401Phosphoserine; by CaMK2 and PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24529.
PaxDbiP24529.
PRIDEiP24529.

PTM databases

PhosphoSiteiP24529.

Expressioni

Gene expression databases

BgeeiP24529.
CleanExiMM_TH.
ExpressionAtlasiP24529. baseline and differential.
GenevisibleiP24529. MM.

Interactioni

Protein-protein interaction databases

IntActiP24529. 1 interaction.
MINTiMINT-4138908.
STRINGi10090.ENSMUSP00000000219.

Structurei

3D structure databases

ProteinModelPortaliP24529.
SMRiP24529. Positions 65-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 599Poly-Ala

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP24529.
KOiK00501.
OMAiYATHACG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP24529.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEiPS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTPSASSPQ PKGFRRAVSE QDTKQAEAVT SPRFIGRRQS LIEDARKERE
60 70 80 90 100
AAAAAAAAAV ASAEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA
110 120 130 140 150
LKVFETFEAK IHHLETRPAQ RPLAGSPHLE YFVRFEVPSG DLAALLSSVR
160 170 180 190 200
RVSDDVRSAR EDKVPWFPRK VSELDKCHHL VTKFDPDLDL DHPGFSDQAY
210 220 230 240 250
RQRRKLIAEI AFQYKQGEPI PHVEYTKEEI ATWKEVYATL KGLYATHACR
260 270 280 290 300
EHLEAFQLLE RYCGYREDSI PQLEDVSHFL KERTGFQLRP VAGLLSARDF
310 320 330 340 350
LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ
360 370 380 390 400
DIGLASLGAS DEEIEKLSTV YWFTVEFGLC KQNGELKAYG AGLLSSYGEL
410 420 430 440 450
LHSLSEEPEV RAFDPDTAAV QPYQDQTYQP VYFVSESFSD AKDKLRNYAS
460 470 480 490
RIQRPFSVKF DPYTLAIDVL DSPHTIRRSL EGVQDELHTL TQALSAIS
Length:498
Mass (Da):55,993
Last modified:January 23, 2007 - v3
Checksum:i62790179664F6DC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69200 mRNA. Translation: AAA40434.1.
X53503 Genomic DNA. Translation: CAA37580.1.
CCDSiCCDS22036.1.
PIRiJN0068.
RefSeqiNP_033403.1. NM_009377.1.
UniGeneiMm.1292.

Genome annotation databases

EnsembliENSMUST00000000219; ENSMUSP00000000219; ENSMUSG00000000214.
GeneIDi21823.
KEGGimmu:21823.
UCSCiuc009koi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69200 mRNA. Translation: AAA40434.1.
X53503 Genomic DNA. Translation: CAA37580.1.
CCDSiCCDS22036.1.
PIRiJN0068.
RefSeqiNP_033403.1. NM_009377.1.
UniGeneiMm.1292.

3D structure databases

ProteinModelPortaliP24529.
SMRiP24529. Positions 65-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24529. 1 interaction.
MINTiMINT-4138908.
STRINGi10090.ENSMUSP00000000219.

PTM databases

PhosphoSiteiP24529.

Proteomic databases

MaxQBiP24529.
PaxDbiP24529.
PRIDEiP24529.

Protocols and materials databases

DNASUi21823.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000219; ENSMUSP00000000219; ENSMUSG00000000214.
GeneIDi21823.
KEGGimmu:21823.
UCSCiuc009koi.1. mouse.

Organism-specific databases

CTDi7054.
MGIiMGI:98735. Th.

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP24529.
KOiK00501.
OMAiYATHACG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP24529.
TreeFamiTF313327.

Enzyme and pathway databases

UniPathwayiUPA00747; UER00733.
ReactomeiREACT_295408. Catecholamine biosynthesis.

Miscellaneous databases

NextBioi301244.
PROiP24529.
SOURCEiSearch...

Gene expression databases

BgeeiP24529.
CleanExiMM_TH.
ExpressionAtlasiP24529. baseline and differential.
GenevisibleiP24529. MM.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEiPS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of mouse tyrosine hydroxylase deduced from its cDNA."
    Ichikawa S., Sasaoka T., Nagatsu T.
    Biochem. Biophys. Res. Commun. 176:1610-1616(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Morgan W.W., Bermudez J., Sharp Z.D.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-30.
    Strain: BALB/c.
  3. Lubec G., Yang J.W., Zigmond M.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 78-90.
    Tissue: Brain.

Entry informationi

Entry nameiTY3H_MOUSE
AccessioniPrimary (citable) accession number: P24529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.