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Protein

Tyrosine 3-monooxygenase

Gene

Th

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the physiology of adrenergic neurons.

Catalytic activityi

L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactori

Enzyme regulationi

Phosphorylation leads to an increase in the catalytic activity.

Pathwayi: dopamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dopamine from L-tyrosine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tyrosine 3-monooxygenase (Th)
  2. Aromatic-L-amino-acid decarboxylase (Ddc)
This subpathway is part of the pathway dopamine biosynthesis, which is itself part of Catecholamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dopamine from L-tyrosine, the pathway dopamine biosynthesis and in Catecholamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi331IronBy similarity1
Metal bindingi336IronBy similarity1
Metal bindingi376IronBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aminergic neurotransmitter loading into synaptic vesicle Source: MGI
  • animal organ morphogenesis Source: MGI
  • catecholamine biosynthetic process Source: MGI
  • cellular response to alkaloid Source: Ensembl
  • cellular response to glucose stimulus Source: Ensembl
  • cellular response to growth factor stimulus Source: Ensembl
  • cellular response to manganese ion Source: Ensembl
  • cellular response to nicotine Source: Ensembl
  • cerebral cortex development Source: Ensembl
  • circadian sleep/wake cycle Source: Ensembl
  • dopamine biosynthetic process Source: MGI
  • dopamine biosynthetic process from tyrosine Source: MGI
  • eating behavior Source: MGI
  • embryonic camera-type eye morphogenesis Source: BHF-UCL
  • epinephrine biosynthetic process Source: MGI
  • eye photoreceptor cell development Source: BHF-UCL
  • fatty acid metabolic process Source: Ensembl
  • glycoside metabolic process Source: Ensembl
  • heart development Source: MGI
  • isoquinoline alkaloid metabolic process Source: Ensembl
  • learning Source: MGI
  • locomotory behavior Source: MGI
  • mating behavior Source: MGI
  • memory Source: MGI
  • multicellular organism aging Source: Ensembl
  • neurotransmitter biosynthetic process Source: UniProtKB-KW
  • norepinephrine biosynthetic process Source: MGI
  • phthalate metabolic process Source: Ensembl
  • phytoalexin metabolic process Source: Ensembl
  • regulation of heart contraction Source: MGI
  • response to activity Source: Ensembl
  • response to amphetamine Source: Ensembl
  • response to corticosterone Source: Ensembl
  • response to electrical stimulus Source: Ensembl
  • response to estradiol Source: Ensembl
  • response to ethanol Source: MGI
  • response to ether Source: Ensembl
  • response to herbicide Source: Ensembl
  • response to hypoxia Source: MGI
  • response to immobilization stress Source: Ensembl
  • response to isolation stress Source: Ensembl
  • response to light stimulus Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to nutrient levels Source: Ensembl
  • response to peptide hormone Source: Ensembl
  • response to pyrethroid Source: Ensembl
  • response to salt stress Source: Ensembl
  • response to water deprivation Source: Ensembl
  • response to zinc ion Source: Ensembl
  • sensory perception of sound Source: Ensembl
  • social behavior Source: Ensembl
  • sphingolipid metabolic process Source: Ensembl
  • synaptic transmission, dopaminergic Source: MGI
  • terpene metabolic process Source: Ensembl
  • visual perception Source: BHF-UCL

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processCatecholamine biosynthesis, Neurotransmitter biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-209905 Catecholamine biosynthesis
UniPathwayiUPA00747; UER00733

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine 3-monooxygenase (EC:1.14.16.2)
Alternative name(s):
Tyrosine 3-hydroxylase
Short name:
TH
Gene namesi
Name:Th
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98735 Th

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055621 – 498Tyrosine 3-monooxygenaseAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineCombined sources1
Modified residuei31PhosphoserineCombined sources1
Modified residuei40Phosphoserine; by CaMK2 and PKABy similarity1
Modified residuei472PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24529
PaxDbiP24529
PeptideAtlasiP24529
PRIDEiP24529

PTM databases

iPTMnetiP24529
PhosphoSitePlusiP24529

Expressioni

Gene expression databases

BgeeiENSMUSG00000000214
CleanExiMM_TH
ExpressionAtlasiP24529 baseline and differential
GenevisibleiP24529 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP24529, 1 interactor
MINTiP24529
STRINGi10090.ENSMUSP00000000219

Structurei

3D structure databases

ProteinModelPortaliP24529
SMRiP24529
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 59Poly-Ala9

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3820 Eukaryota
COG3186 LUCA
GeneTreeiENSGT00390000010268
HOGENOMiHOG000233373
HOVERGENiHBG006841
InParanoidiP24529
KOiK00501
OMAiPHLEYFV
OrthoDBiEOG091G05MZ
PhylomeDBiP24529
TreeFamiTF313327

Family and domain databases

Gene3Di1.10.800.10, 1 hit
InterProiView protein in InterPro
IPR001273 ArAA_hydroxylase
IPR018301 ArAA_hydroxylase_Fe/CU_BS
IPR036951 ArAA_hydroxylase_sf
IPR036329 Aro-AA_hydroxylase_C_sf
IPR019774 Aromatic-AA_hydroxylase_C
IPR005962 Tyr_3_mOase
IPR019773 Tyrosine_3-monooxygenase-like
IPR021164 Tyrosine_hydroxylase_CS
PANTHERiPTHR11473 PTHR11473, 1 hit
PfamiView protein in Pfam
PF00351 Biopterin_H, 1 hit
PF12549 TOH_N, 2 hits
PIRSFiPIRSF000336 TH, 1 hit
PRINTSiPR00372 FYWHYDRXLASE
SUPFAMiSSF56534 SSF56534, 1 hit
TIGRFAMsiTIGR01269 Tyr_3_monoox, 1 hit
PROSITEiView protein in PROSITE
PS00367 BH4_AAA_HYDROXYL_1, 1 hit
PS51410 BH4_AAA_HYDROXYL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P24529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTPSASSPQ PKGFRRAVSE QDTKQAEAVT SPRFIGRRQS LIEDARKERE
60 70 80 90 100
AAAAAAAAAV ASAEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA
110 120 130 140 150
LKVFETFEAK IHHLETRPAQ RPLAGSPHLE YFVRFEVPSG DLAALLSSVR
160 170 180 190 200
RVSDDVRSAR EDKVPWFPRK VSELDKCHHL VTKFDPDLDL DHPGFSDQAY
210 220 230 240 250
RQRRKLIAEI AFQYKQGEPI PHVEYTKEEI ATWKEVYATL KGLYATHACR
260 270 280 290 300
EHLEAFQLLE RYCGYREDSI PQLEDVSHFL KERTGFQLRP VAGLLSARDF
310 320 330 340 350
LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ
360 370 380 390 400
DIGLASLGAS DEEIEKLSTV YWFTVEFGLC KQNGELKAYG AGLLSSYGEL
410 420 430 440 450
LHSLSEEPEV RAFDPDTAAV QPYQDQTYQP VYFVSESFSD AKDKLRNYAS
460 470 480 490
RIQRPFSVKF DPYTLAIDVL DSPHTIRRSL EGVQDELHTL TQALSAIS
Length:498
Mass (Da):55,993
Last modified:January 23, 2007 - v3
Checksum:i62790179664F6DC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69200 mRNA Translation: AAA40434.1
X53503 Genomic DNA Translation: CAA37580.1
CCDSiCCDS22036.1
PIRiJN0068
RefSeqiNP_033403.1, NM_009377.1
UniGeneiMm.1292

Genome annotation databases

EnsembliENSMUST00000000219; ENSMUSP00000000219; ENSMUSG00000000214
GeneIDi21823
KEGGimmu:21823
UCSCiuc009koi.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTY3H_MOUSE
AccessioniPrimary (citable) accession number: P24529
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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