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P24529 (TY3H_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine 3-monooxygenase

EC=1.14.16.2
Alternative name(s):
Tyrosine 3-hydroxylase
Short name=TH
Gene names
Name:Th
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in the physiology of adrenergic neurons.

Catalytic activity

L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactor

Fe2+ ion.

Enzyme regulation

Phosphorylation leads to an increase in the catalytic activity.

Pathway

Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.

Sequence similarities

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
   Biological processCatecholamine biosynthesis
Neurotransmitter biosynthesis
   LigandIron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to drug

Inferred from electronic annotation. Source: Ensembl

cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to manganese ion

Inferred from electronic annotation. Source: Ensembl

cellular response to nicotine

Inferred from electronic annotation. Source: Ensembl

cerebral cortex development

Inferred from electronic annotation. Source: Ensembl

circadian sleep/wake cycle

Inferred from electronic annotation. Source: Ensembl

dopamine biosynthetic process from tyrosine

Inferred from direct assay PubMed 9520487. Source: MGI

eating behavior

Inferred from mutant phenotype PubMed 11430814PubMed 15374756. Source: MGI

embryonic camera-type eye morphogenesis

Inferred from mutant phenotype PubMed 16445854. Source: BHF-UCL

epinephrine biosynthetic process

Inferred from electronic annotation. Source: Ensembl

eye photoreceptor cell development

Inferred from mutant phenotype PubMed 16445854. Source: BHF-UCL

fatty acid metabolic process

Inferred from electronic annotation. Source: Ensembl

glycoside metabolic process

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from mutant phenotype PubMed 10212311PubMed 7715703. Source: MGI

isoquinoline alkaloid metabolic process

Inferred from electronic annotation. Source: Ensembl

learning

Inferred from mutant phenotype PubMed 14684249. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 11430814PubMed 14684249. Source: MGI

mating behavior

Inferred from mutant phenotype PubMed 9829800. Source: MGI

memory

Inferred from mutant phenotype PubMed 14684249. Source: MGI

multicellular organismal aging

Inferred from electronic annotation. Source: Ensembl

neurotransmitter biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

norepinephrine biosynthetic process

Inferred from electronic annotation. Source: Ensembl

organ morphogenesis

Inferred from mutant phenotype PubMed 7715703. Source: MGI

phthalate metabolic process

Inferred from electronic annotation. Source: Ensembl

phytoalexin metabolic process

Inferred from electronic annotation. Source: Ensembl

regulation of heart contraction

Inferred from mutant phenotype PubMed 7592982PubMed 7715703. Source: MGI

response to activity

Inferred from electronic annotation. Source: Ensembl

response to amphetamine

Inferred from electronic annotation. Source: Ensembl

response to corticosterone

Inferred from electronic annotation. Source: Ensembl

response to electrical stimulus

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to ether

Inferred from electronic annotation. Source: Ensembl

response to herbicide

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to light stimulus

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to nutrient levels

Inferred from electronic annotation. Source: Ensembl

response to peptide hormone

Inferred from electronic annotation. Source: Ensembl

response to pyrethroid

Inferred from electronic annotation. Source: Ensembl

response to salt stress

Inferred from electronic annotation. Source: Ensembl

response to water deprivation

Inferred from electronic annotation. Source: Ensembl

response to zinc ion

Inferred from electronic annotation. Source: Ensembl

sensory perception of sound

Inferred from electronic annotation. Source: Ensembl

social behavior

Inferred from electronic annotation. Source: Ensembl

sphingolipid metabolic process

Inferred from electronic annotation. Source: Ensembl

synaptic transmission, dopaminergic

Inferred from mutant phenotype PubMed 12604788PubMed 15317940. Source: MGI

synaptic vesicle amine transport

Inferred from electronic annotation. Source: Ensembl

terpene metabolic process

Inferred from electronic annotation. Source: Ensembl

visual perception

Inferred from mutant phenotype PubMed 16445854. Source: BHF-UCL

   Cellular_componentaxon

Inferred from direct assay PubMed 15814794PubMed 17761882. Source: MGI

cytoplasm

Inferred from direct assay PubMed 10707987. Source: MGI

cytoplasmic side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from electronic annotation. Source: Ensembl

melanosome membrane

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Inferred from electronic annotation. Source: Ensembl

neuron projection

Inferred from direct assay PubMed 12074840. Source: MGI

nucleus

Inferred from direct assay PubMed 15643613. Source: MGI

perikaryon

Inferred from direct assay PubMed 10350535. Source: MGI

smooth endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle

Inferred from electronic annotation. Source: Ensembl

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionamino acid binding

Inferred from electronic annotation. Source: Ensembl

dopamine binding

Inferred from electronic annotation. Source: Ensembl

ferric iron binding

Inferred from electronic annotation. Source: Ensembl

ferrous iron binding

Inferred from electronic annotation. Source: Ensembl

oxygen binding

Inferred from electronic annotation. Source: Ensembl

tetrahydrobiopterin binding

Inferred from electronic annotation. Source: Ensembl

tyrosine 3-monooxygenase activity

Traceable author statement PubMed 7592982. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 498497Tyrosine 3-monooxygenase
PRO_0000205562

Regions

Compositional bias51 – 599Poly-Ala

Sites

Metal binding3311Iron By similarity
Metal binding3361Iron By similarity
Metal binding3761Iron By similarity

Amino acid modifications

Modified residue191Phosphoserine; by CaMK2 By similarity
Modified residue311Phosphoserine By similarity
Modified residue401Phosphoserine; by CaMK2 and PKA By similarity

Sequences

Sequence LengthMass (Da)Tools
P24529 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 62790179664F6DC6

FASTA49855,993
        10         20         30         40         50         60 
MPTPSASSPQ PKGFRRAVSE QDTKQAEAVT SPRFIGRRQS LIEDARKERE AAAAAAAAAV 

        70         80         90        100        110        120 
ASAEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA LKVFETFEAK IHHLETRPAQ 

       130        140        150        160        170        180 
RPLAGSPHLE YFVRFEVPSG DLAALLSSVR RVSDDVRSAR EDKVPWFPRK VSELDKCHHL 

       190        200        210        220        230        240 
VTKFDPDLDL DHPGFSDQAY RQRRKLIAEI AFQYKQGEPI PHVEYTKEEI ATWKEVYATL 

       250        260        270        280        290        300 
KGLYATHACR EHLEAFQLLE RYCGYREDSI PQLEDVSHFL KERTGFQLRP VAGLLSARDF 

       310        320        330        340        350        360 
LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ DIGLASLGAS 

       370        380        390        400        410        420 
DEEIEKLSTV YWFTVEFGLC KQNGELKAYG AGLLSSYGEL LHSLSEEPEV RAFDPDTAAV 

       430        440        450        460        470        480 
QPYQDQTYQP VYFVSESFSD AKDKLRNYAS RIQRPFSVKF DPYTLAIDVL DSPHTIRRSL 

       490 
EGVQDELHTL TQALSAIS 

« Hide

References

[1]"Primary structure of mouse tyrosine hydroxylase deduced from its cDNA."
Ichikawa S., Sasaoka T., Nagatsu T.
Biochem. Biophys. Res. Commun. 176:1610-1616(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Morgan W.W., Bermudez J., Sharp Z.D.
Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-30.
Strain: BALB/c.
[3]Lubec G., Yang J.W., Zigmond M.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 78-90.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M69200 mRNA. Translation: AAA40434.1.
X53503 Genomic DNA. Translation: CAA37580.1.
PIRJN0068.
RefSeqNP_033403.1. NM_009377.1.
UniGeneMm.1292.

3D structure databases

ProteinModelPortalP24529.
SMRP24529. Positions 80-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP24529. 1 interaction.
MINTMINT-4138908.
STRING10090.ENSMUSP00000000219.

PTM databases

PhosphoSiteP24529.

Proteomic databases

PaxDbP24529.
PRIDEP24529.

Protocols and materials databases

DNASU21823.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000219; ENSMUSP00000000219; ENSMUSG00000000214.
GeneID21823.
KEGGmmu:21823.
UCSCuc009koi.1. mouse.

Organism-specific databases

CTD7054.
MGIMGI:98735. Th.

Phylogenomic databases

eggNOGCOG3186.
GeneTreeENSGT00390000010268.
HOGENOMHOG000233373.
HOVERGENHBG006841.
InParanoidP24529.
KOK00501.
OMAELDKCHH.
OrthoDBEOG7KM5T1.
PhylomeDBP24529.
TreeFamTF313327.

Enzyme and pathway databases

UniPathwayUPA00747; UER00733.

Gene expression databases

BgeeP24529.
CleanExMM_TH.
GenevestigatorP24529.

Family and domain databases

Gene3D1.10.800.10. 1 hit.
InterProIPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERPTHR11473. PTHR11473. 1 hit.
PfamPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFPIRSF000336. TH. 1 hit.
PRINTSPR00372. FYWHYDRXLASE.
SUPFAMSSF56534. SSF56534. 1 hit.
TIGRFAMsTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEPS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301244.
PROP24529.
SOURCESearch...

Entry information

Entry nameTY3H_MOUSE
AccessionPrimary (citable) accession number: P24529
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot