Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine 3-monooxygenase

Gene

Th

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the physiology of adrenergic neurons.

Catalytic activityi

L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactori

Enzyme regulationi

Phosphorylation leads to an increase in the catalytic activity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi331 – 3311IronBy similarity
Metal bindingi336 – 3361IronBy similarity
Metal bindingi376 – 3761IronBy similarity

GO - Molecular functioni

  1. amino acid binding Source: Ensembl
  2. dopamine binding Source: Ensembl
  3. enzyme binding Source: MGI
  4. ferric iron binding Source: Ensembl
  5. ferrous iron binding Source: Ensembl
  6. oxygen binding Source: Ensembl
  7. tetrahydrobiopterin binding Source: Ensembl
  8. tyrosine 3-monooxygenase activity Source: MGI

GO - Biological processi

  1. cellular response to drug Source: Ensembl
  2. cellular response to glucose stimulus Source: Ensembl
  3. cellular response to growth factor stimulus Source: Ensembl
  4. cellular response to manganese ion Source: Ensembl
  5. cellular response to nicotine Source: Ensembl
  6. cerebral cortex development Source: Ensembl
  7. circadian sleep/wake cycle Source: Ensembl
  8. dopamine biosynthetic process Source: MGI
  9. dopamine biosynthetic process from tyrosine Source: MGI
  10. eating behavior Source: MGI
  11. embryonic camera-type eye morphogenesis Source: BHF-UCL
  12. epinephrine biosynthetic process Source: MGI
  13. eye photoreceptor cell development Source: BHF-UCL
  14. fatty acid metabolic process Source: Ensembl
  15. glycoside metabolic process Source: Ensembl
  16. heart development Source: MGI
  17. isoquinoline alkaloid metabolic process Source: Ensembl
  18. learning Source: MGI
  19. locomotory behavior Source: MGI
  20. mating behavior Source: MGI
  21. memory Source: MGI
  22. multicellular organismal aging Source: Ensembl
  23. neurotransmitter biosynthetic process Source: UniProtKB-KW
  24. norepinephrine biosynthetic process Source: MGI
  25. organ morphogenesis Source: MGI
  26. phthalate metabolic process Source: Ensembl
  27. phytoalexin metabolic process Source: Ensembl
  28. regulation of heart contraction Source: MGI
  29. response to activity Source: Ensembl
  30. response to amphetamine Source: Ensembl
  31. response to corticosterone Source: Ensembl
  32. response to electrical stimulus Source: Ensembl
  33. response to estradiol Source: Ensembl
  34. response to ethanol Source: MGI
  35. response to ether Source: Ensembl
  36. response to herbicide Source: Ensembl
  37. response to hypoxia Source: MGI
  38. response to immobilization stress Source: Ensembl
  39. response to light stimulus Source: Ensembl
  40. response to lipopolysaccharide Source: Ensembl
  41. response to nutrient levels Source: Ensembl
  42. response to peptide hormone Source: Ensembl
  43. response to pyrethroid Source: Ensembl
  44. response to salt stress Source: Ensembl
  45. response to water deprivation Source: Ensembl
  46. response to zinc ion Source: Ensembl
  47. sensory perception of sound Source: Ensembl
  48. social behavior Source: Ensembl
  49. sphingolipid metabolic process Source: Ensembl
  50. synaptic transmission, dopaminergic Source: MGI
  51. synaptic vesicle amine transport Source: Ensembl
  52. terpene metabolic process Source: Ensembl
  53. visual perception Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Catecholamine biosynthesis, Neurotransmitter biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_217777. Catecholamine biosynthesis.
UniPathwayiUPA00747; UER00733.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine 3-monooxygenase (EC:1.14.16.2)
Alternative name(s):
Tyrosine 3-hydroxylase
Short name:
TH
Gene namesi
Name:Th
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:98735. Th.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: MGI
  2. cytoplasm Source: MGI
  3. cytoplasmic side of plasma membrane Source: MGI
  4. cytoplasmic vesicle Source: MGI
  5. cytosol Source: Ensembl
  6. dendrite Source: Ensembl
  7. melanosome membrane Source: MGI
  8. mitochondrion Source: Ensembl
  9. neuron projection Source: MGI
  10. nucleus Source: MGI
  11. perikaryon Source: MGI
  12. smooth endoplasmic reticulum Source: MGI
  13. synaptic vesicle Source: Ensembl
  14. terminal bouton Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 498497Tyrosine 3-monooxygenasePRO_0000205562Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine; by CaMK2By similarity
Modified residuei31 – 311PhosphoserineBy similarity
Modified residuei40 – 401Phosphoserine; by CaMK2 and PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24529.
PaxDbiP24529.
PRIDEiP24529.

PTM databases

PhosphoSiteiP24529.

Expressioni

Gene expression databases

BgeeiP24529.
CleanExiMM_TH.
ExpressionAtlasiP24529. baseline and differential.
GenevestigatoriP24529.

Interactioni

Protein-protein interaction databases

IntActiP24529. 1 interaction.
MINTiMINT-4138908.
STRINGi10090.ENSMUSP00000000219.

Structurei

3D structure databases

ProteinModelPortaliP24529.
SMRiP24529. Positions 65-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 599Poly-Ala

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP24529.
KOiK00501.
OMAiYATHACG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP24529.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEiPS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTPSASSPQ PKGFRRAVSE QDTKQAEAVT SPRFIGRRQS LIEDARKERE
60 70 80 90 100
AAAAAAAAAV ASAEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA
110 120 130 140 150
LKVFETFEAK IHHLETRPAQ RPLAGSPHLE YFVRFEVPSG DLAALLSSVR
160 170 180 190 200
RVSDDVRSAR EDKVPWFPRK VSELDKCHHL VTKFDPDLDL DHPGFSDQAY
210 220 230 240 250
RQRRKLIAEI AFQYKQGEPI PHVEYTKEEI ATWKEVYATL KGLYATHACR
260 270 280 290 300
EHLEAFQLLE RYCGYREDSI PQLEDVSHFL KERTGFQLRP VAGLLSARDF
310 320 330 340 350
LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ
360 370 380 390 400
DIGLASLGAS DEEIEKLSTV YWFTVEFGLC KQNGELKAYG AGLLSSYGEL
410 420 430 440 450
LHSLSEEPEV RAFDPDTAAV QPYQDQTYQP VYFVSESFSD AKDKLRNYAS
460 470 480 490
RIQRPFSVKF DPYTLAIDVL DSPHTIRRSL EGVQDELHTL TQALSAIS
Length:498
Mass (Da):55,993
Last modified:January 23, 2007 - v3
Checksum:i62790179664F6DC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69200 mRNA. Translation: AAA40434.1.
X53503 Genomic DNA. Translation: CAA37580.1.
CCDSiCCDS22036.1.
PIRiJN0068.
RefSeqiNP_033403.1. NM_009377.1.
UniGeneiMm.1292.

Genome annotation databases

EnsembliENSMUST00000000219; ENSMUSP00000000219; ENSMUSG00000000214.
GeneIDi21823.
KEGGimmu:21823.
UCSCiuc009koi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69200 mRNA. Translation: AAA40434.1.
X53503 Genomic DNA. Translation: CAA37580.1.
CCDSiCCDS22036.1.
PIRiJN0068.
RefSeqiNP_033403.1. NM_009377.1.
UniGeneiMm.1292.

3D structure databases

ProteinModelPortaliP24529.
SMRiP24529. Positions 65-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24529. 1 interaction.
MINTiMINT-4138908.
STRINGi10090.ENSMUSP00000000219.

PTM databases

PhosphoSiteiP24529.

Proteomic databases

MaxQBiP24529.
PaxDbiP24529.
PRIDEiP24529.

Protocols and materials databases

DNASUi21823.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000219; ENSMUSP00000000219; ENSMUSG00000000214.
GeneIDi21823.
KEGGimmu:21823.
UCSCiuc009koi.1. mouse.

Organism-specific databases

CTDi7054.
MGIiMGI:98735. Th.

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP24529.
KOiK00501.
OMAiYATHACG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP24529.
TreeFamiTF313327.

Enzyme and pathway databases

UniPathwayiUPA00747; UER00733.
ReactomeiREACT_217777. Catecholamine biosynthesis.

Miscellaneous databases

NextBioi301244.
PROiP24529.
SOURCEiSearch...

Gene expression databases

BgeeiP24529.
CleanExiMM_TH.
ExpressionAtlasiP24529. baseline and differential.
GenevestigatoriP24529.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEiPS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of mouse tyrosine hydroxylase deduced from its cDNA."
    Ichikawa S., Sasaoka T., Nagatsu T.
    Biochem. Biophys. Res. Commun. 176:1610-1616(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Morgan W.W., Bermudez J., Sharp Z.D.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-30.
    Strain: BALB/c.
  3. Lubec G., Yang J.W., Zigmond M.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 78-90.
    Tissue: Brain.

Entry informationi

Entry nameiTY3H_MOUSE
AccessioniPrimary (citable) accession number: P24529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.