Reviewed, UniProtKB/Swiss-Prot P24529 (TY3H_MOUSE)

Names and origin

Protein names

Recommended name:
    Tyrosine 3-monooxygenase
    EC=1.14.16.2
Alternative name(s):
    Tyrosine 3-hydroxylase
      Short name=TH

Gene names

Name:

Th

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays an important role in the physiology of adrenergic neurons.
Catalytic activity	L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Enzyme regulation	Phosphorylation leads to an increase in the catalytic activity.
Pathway	Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Catecholamine biosynthesis Neurotransmitter biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	dopamine biosynthetic process from tyrosine Inferred from direct assay. Source: MGI eating behavior Inferred from mutant phenotype. Source: MGI embryonic camera-type eye morphogenesis Inferred from mutant phenotype. Source: UniProtKB eye photoreceptor cell development Inferred from mutant phenotype. Source: UniProtKB heart development Inferred from mutant phenotype. Source: MGI learning Inferred from mutant phenotype. Source: MGI locomotory behavior Inferred from mutant phenotype. Source: MGI mating behavior Inferred from mutant phenotype. Source: MGI memory Inferred from mutant phenotype. Source: MGI neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of heart contraction Inferred from mutant phenotype. Source: MGI synaptic transmission, dopaminergic Inferred from mutant phenotype. Source: MGI visual perception Inferred from mutant phenotype. Source: UniProtKB
Cellular component	nucleus Inferred from direct assay. Source: MGI perikaryon Inferred from direct assay. Source: MGI
Molecular function	tyrosine 3-monooxygenase activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 498	497	Tyrosine 3-monooxygenase		PRO_0000205562
Regions
	Compositional bias	51 – 59	9	Poly-Ala
Sites
	Metal binding	331	1	Iron By similarity
	Metal binding	336	1	Iron By similarity
	Metal binding	376	1	Iron By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine; by CaMK2 By similarity
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	40	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24529-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 62790179664F6DC6	FASTA	498	55,993
	10 20 30 40 50 60 MPTPSASSPQ PKGFRRAVSE QDTKQAEAVT SPRFIGRRQS LIEDARKERE AAAAAAAAAV 70 80 90 100 110 120 ASAEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA LKVFETFEAK IHHLETRPAQ 130 140 150 160 170 180 RPLAGSPHLE YFVRFEVPSG DLAALLSSVR RVSDDVRSAR EDKVPWFPRK VSELDKCHHL 190 200 210 220 230 240 VTKFDPDLDL DHPGFSDQAY RQRRKLIAEI AFQYKQGEPI PHVEYTKEEI ATWKEVYATL 250 260 270 280 290 300 KGLYATHACR EHLEAFQLLE RYCGYREDSI PQLEDVSHFL KERTGFQLRP VAGLLSARDF 310 320 330 340 350 360 LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ DIGLASLGAS 370 380 390 400 410 420 DEEIEKLSTV YWFTVEFGLC KQNGELKAYG AGLLSSYGEL LHSLSEEPEV RAFDPDTAAV 430 440 450 460 470 480 QPYQDQTYQP VYFVSESFSD AKDKLRNYAS RIQRPFSVKF DPYTLAIDVL DSPHTIRRSL 490 EGVQDELHTL TQALSAIS

References

[1]	"Primary structure of mouse tyrosine hydroxylase deduced from its cDNA." Ichikawa S., Sasaoka T., Nagatsu T. Biochem. Biophys. Res. Commun. 176:1610-1616(1991) [PubMed: 1674869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Morgan W.W., Bermudez J., Sharp Z.D. Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-30. Strain: BALB/c.
[3]	Lubec G., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 78-90. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M69200 mRNA. Translation: AAA40434.1. X53503 Genomic DNA. Translation: CAA37580.1.
IPI	IPI00138131.
PIR	JN0068.
RefSeq	NP_033403.1.
UniGene	Mm.1292
3D structure databases
HSSP	HSSP built from PDB template 1TOH based on UniProtKB P04177.
SMR	P24529. Positions 159-497, 160-498.
ModBase	Search...
PTM databases
PhosphoSite	P24529.
Proteomic databases
PRIDE	P24529.
Genome annotation databases
Ensembl	ENSMUSG00000000214. Mus musculus. [Contig view]
GeneID	21823.
KEGG	mmu:21823.
Organism-specific databases
MGI	MGI:98735. Th.
Phylogenomic databases
HOGENOM	P24529.
HOVERGEN	P24529.
OMA	P24529. ELDKCHH.
Enzyme and pathway databases
BRENDA	1.14.16.2. 244.
Gene expression databases
ArrayExpress	P24529.
Bgee	P24529.
CleanEx	MM_TH.
GermOnline	ENSMUSG00000000214. Mus musculus.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005962. Tyr_3_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01269. Tyr_3_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301244.
SOURCE	Search...

Entry information

Entry name

TY3H_MOUSE

Accession

Primary (citable) accession number: P24529

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)