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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

Mgmt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5ZincBy similarity1
Metal bindingi24ZincBy similarity1
Metal bindingi29ZincBy similarity1
Metal bindingi89ZincBy similarity1
Binding sitei99DNA; via amide nitrogenBy similarity1
Binding sitei118DNABy similarity1
Binding sitei119DNA; via amide nitrogenBy similarity1
Binding sitei127DNABy similarity1
Binding sitei132DNABy similarity1
Active sitei149Nucleophile; methyl group acceptorPROSITE-ProRule annotation1
Binding sitei155DNA; via amide nitrogenBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • DNA binding Source: UniProtKB-KW
  • methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: RGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: RGD
  • cellular response to ionizing radiation Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to oxidative stress Source: RGD
  • DNA dealkylation involved in DNA repair Source: RGD
  • mammary gland epithelial cell differentiation Source: RGD
  • negative regulation of apoptotic process Source: Ensembl
  • negative regulation of cell death Source: RGD
  • positive regulation of DNA repair Source: RGD
  • positive regulation of double-strand break repair Source: Ensembl
  • regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  • response to drug Source: RGD
  • response to ethanol Source: RGD
  • response to folic acid Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to toxic substance Source: RGD

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:Mgmt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3087. Mgmt.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393611 – 209Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST209

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP24528.
PRIDEiP24528.

PTM databases

iPTMnetiP24528.
PhosphoSitePlusiP24528.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016038.
GenevisibleiP24528. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021537.

Structurei

3D structure databases

ProteinModelPortaliP24528.
SMRiP24528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiKOG4062. Eukaryota.
COG0350. LUCA.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP24528.
KOiK00567.
OMAiMDKACEM.
OrthoDBiEOG091G11SR.
PhylomeDBiP24528.
TreeFamiTF314064.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

P24528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEICKMKYT VLDSPLGKIE LSGCERGLHG IRFLSGKTPN TDPTEAPACP
60 70 80 90 100
EVLGGPEGVP EPLVQCTAWL EAYFHEPAAT EGLPLPALHH PVFQQDSFTR
110 120 130 140 150
QVLWKLLKVV KFGEMVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH
160 170 180 190 200
RVIRSDGAIG NYSGGGQTVK EWLLAHEGIP TGQPASKGLG LIGSWLKPSF

ESSSPKPSG
Length:209
Mass (Da):22,244
Last modified:January 23, 2007 - v3
Checksum:i61F8F51C8937D4A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S61804 mRNA. Translation: AAB20187.1.
M76704 mRNA. Translation: AAA42052.1.
X54862 mRNA. Translation: CAA38648.1.
PIRiJN0071. XURTMC.
RefSeqiNP_036993.1. NM_012861.1.
UniGeneiRn.9836.

Genome annotation databases

EnsembliENSRNOT00000021537; ENSRNOP00000021537; ENSRNOG00000016038.
GeneIDi25332.
KEGGirno:25332.

Similar proteinsi

Entry informationi

Entry nameiMGMT_RAT
AccessioniPrimary (citable) accession number: P24528
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families