Reviewed, UniProtKB/Swiss-Prot P24528 (MGMT_RAT)

Names and origin

Protein names

Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    EC=2.1.1.63
Alternative name(s):
    6-O-methylguanine-DNA methyltransferase
      Short name=MGMT
    O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:

Mgmt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 209	208	Methylated-DNA--protein-cysteine methyltransferase		PRO_0000139361
Sites
	Active site	149	1	Nucleophile; methyl group acceptor By similarity
	Metal binding	5	1	Zinc By similarity
	Metal binding	24	1	Zinc By similarity
	Metal binding	29	1	Zinc By similarity
	Metal binding	89	1	Zinc By similarity
	Binding site	99	1	DNA; via amide nitrogen By similarity
	Binding site	118	1	DNA By similarity
	Binding site	119	1	DNA; via amide nitrogen By similarity
	Binding site	127	1	DNA By similarity
	Binding site	132	1	DNA By similarity
	Binding site	155	1	DNA; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	10	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24528-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 61F8F51C8937D4A7	FASTA	209	22,244
	10 20 30 40 50 60 MAEICKMKYT VLDSPLGKIE LSGCERGLHG IRFLSGKTPN TDPTEAPACP EVLGGPEGVP 70 80 90 100 110 120 EPLVQCTAWL EAYFHEPAAT EGLPLPALHH PVFQQDSFTR QVLWKLLKVV KFGEMVSYQQ 130 140 150 160 170 180 LAALAGNPKA ARAVGGAMRS NPVPILIPCH RVIRSDGAIG NYSGGGQTVK EWLLAHEGIP 190 200 TGQPASKGLG LIGSWLKPSF ESSSPKPSG

References

[1]	"cDNA cloning of the rat O6-methylguanine-DNA-methyltransferase." Rahden-Staron I., Laval F. Biochem. Biophys. Res. Commun. 177:597-602(1991) [PubMed: 2049083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and cDNA cloning of a rat O6-alkylguanine-DNA-alkyltransferase gene, molecular analysis of expression in rat liver." Potter P.M., Rafferty J.A., Cawkwell L., Wilkinson M.C., Cooper D.P., O'Connor P.J., Margison G.P. Carcinogenesis 12:727-733(1991) [PubMed: 2013136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Ribozyme-mediated modulation of human O6-methylguanine-DNA methyltransferase expression." Potter P.M., Harris L.C., Remack J.S., Edwards C.C., Brent T.P. Cancer Res. 53:1731-1734(1993) [PubMed: 8467487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning and expresion of cDNA for rat O6-methylguanine-DNA methyltransferase." Sakumi K., Shiraishi A., Hayakawa H., Sekiguchi M. Nucleic Acids Res. 19:5597-5601(1991) [PubMed: 1945835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA." Liem L.-K., Lim A., Li B.F.L. Nucleic Acids Res. 22:1613-1619(1994) [PubMed: 8202360] [Abstract] Cited for: CHARACTERIZATION, PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S61804 mRNA. Translation: AAB20187.1. M76704 mRNA. Translation: AAA42052.1. X54862 mRNA. Translation: CAA38648.1.
IPI	IPI00231869.
PIR	XURTMC. JN0071.
RefSeq	NP_036993.1.
UniGene	Rn.9836
3D structure databases
HSSP	HSSP built from PDB template 1EH7 based on UniProtKB P16455.
SMR	P24528. Positions 6-178.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000016038. Rattus norvegicus. [Contig view]
GeneID	25332.
KEGG	rno:25332.
NMPDR	fig|10116.3.peg.3168.
Organism-specific databases
RGD	3087. Mgmt.
Phylogenomic databases
HOVERGEN	P24528.
OMA	P24528. CENGVHT.
Enzyme and pathway databases
BRENDA	2.1.1.63. 248.
Gene expression databases
ArrayExpress	P24528.
GermOnline	ENSRNOG00000016038. Rattus norvegicus.
Family and domain databases
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA_bd. IPR008332. MethylG_MeTrfase. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR10815. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
Pfam	PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00589. ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606215.

Entry information

Entry name

MGMT_RAT

Accession

Primary (citable) accession number: P24528

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)