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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

Mgmt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51ZincBy similarity
Metal bindingi24 – 241ZincBy similarity
Metal bindingi29 – 291ZincBy similarity
Metal bindingi89 – 891ZincBy similarity
Binding sitei99 – 991DNA; via amide nitrogenBy similarity
Binding sitei118 – 1181DNABy similarity
Binding sitei119 – 1191DNA; via amide nitrogenBy similarity
Binding sitei127 – 1271DNABy similarity
Binding sitei132 – 1321DNABy similarity
Active sitei149 – 1491Nucleophile; methyl group acceptorPROSITE-ProRule annotation
Binding sitei155 – 1551DNA; via amide nitrogenBy similarity

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • damaged DNA binding Source: GO_Central
  • methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: RGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: RGD
  • cellular response to ionizing radiation Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to oxidative stress Source: RGD
  • DNA dealkylation involved in DNA repair Source: RGD
  • mammary gland epithelial cell differentiation Source: RGD
  • negative regulation of cell death Source: RGD
  • positive regulation of DNA repair Source: RGD
  • regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  • response to drug Source: RGD
  • response to ethanol Source: RGD
  • response to folic acid Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to toxic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:Mgmt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3087. Mgmt.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Methylated-DNA--protein-cysteine methyltransferasePRO_0000139361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP24528.
PRIDEiP24528.

PTM databases

iPTMnetiP24528.
PhosphoSiteiP24528.

Expressioni

Gene expression databases

GenevisibleiP24528. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021537.

Structurei

3D structure databases

ProteinModelPortaliP24528.
SMRiP24528. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiKOG4062. Eukaryota.
COG0350. LUCA.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP24528.
KOiK00567.
OMAiCENGVHT.
OrthoDBiEOG7RRF9D.
PhylomeDBiP24528.
TreeFamiTF314064.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEICKMKYT VLDSPLGKIE LSGCERGLHG IRFLSGKTPN TDPTEAPACP
60 70 80 90 100
EVLGGPEGVP EPLVQCTAWL EAYFHEPAAT EGLPLPALHH PVFQQDSFTR
110 120 130 140 150
QVLWKLLKVV KFGEMVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH
160 170 180 190 200
RVIRSDGAIG NYSGGGQTVK EWLLAHEGIP TGQPASKGLG LIGSWLKPSF

ESSSPKPSG
Length:209
Mass (Da):22,244
Last modified:January 23, 2007 - v3
Checksum:i61F8F51C8937D4A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S61804 mRNA. Translation: AAB20187.1.
M76704 mRNA. Translation: AAA42052.1.
X54862 mRNA. Translation: CAA38648.1.
PIRiJN0071. XURTMC.
RefSeqiNP_036993.1. NM_012861.1.
UniGeneiRn.9836.

Genome annotation databases

EnsembliENSRNOT00000021537; ENSRNOP00000021537; ENSRNOG00000016038.
GeneIDi25332.
KEGGirno:25332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S61804 mRNA. Translation: AAB20187.1.
M76704 mRNA. Translation: AAA42052.1.
X54862 mRNA. Translation: CAA38648.1.
PIRiJN0071. XURTMC.
RefSeqiNP_036993.1. NM_012861.1.
UniGeneiRn.9836.

3D structure databases

ProteinModelPortaliP24528.
SMRiP24528. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021537.

PTM databases

iPTMnetiP24528.
PhosphoSiteiP24528.

Proteomic databases

PaxDbiP24528.
PRIDEiP24528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021537; ENSRNOP00000021537; ENSRNOG00000016038.
GeneIDi25332.
KEGGirno:25332.

Organism-specific databases

CTDi4255.
RGDi3087. Mgmt.

Phylogenomic databases

eggNOGiKOG4062. Eukaryota.
COG0350. LUCA.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP24528.
KOiK00567.
OMAiCENGVHT.
OrthoDBiEOG7RRF9D.
PhylomeDBiP24528.
TreeFamiTF314064.

Miscellaneous databases

PROiP24528.

Gene expression databases

GenevisibleiP24528. RN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning of the rat O6-methylguanine-DNA-methyltransferase."
    Rahden-Staron I., Laval F.
    Biochem. Biophys. Res. Commun. 177:597-602(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and cDNA cloning of a rat O6-alkylguanine-DNA-alkyltransferase gene, molecular analysis of expression in rat liver."
    Potter P.M., Rafferty J.A., Cawkwell L., Wilkinson M.C., Cooper D.P., O'Connor P.J., Margison G.P.
    Carcinogenesis 12:727-733(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Ribozyme-mediated modulation of human O6-methylguanine-DNA methyltransferase expression."
    Potter P.M., Harris L.C., Remack J.S., Edwards C.C., Brent T.P.
    Cancer Res. 53:1731-1734(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Cloning and expresion of cDNA for rat O6-methylguanine-DNA methyltransferase."
    Sakumi K., Shiraishi A., Hayakawa H., Sekiguchi M.
    Nucleic Acids Res. 19:5597-5601(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA."
    Liem L.-K., Lim A., Li B.F.L.
    Nucleic Acids Res. 22:1613-1619(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiMGMT_RAT
AccessioniPrimary (citable) accession number: P24528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.