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Protein

Phosphomevalonate kinase

Gene

ERG8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 79 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate.

Pathwayi: isopentenyl diphosphate biosynthesis via mevalonate pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Mevalonate kinase (ERG12)
  2. Phosphomevalonate kinase (ERG8)
  3. Diphosphomevalonate decarboxylase (MVD1)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate, the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway and in Isoprenoid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi150 – 160ATPSequence analysisAdd BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphomevalonate kinase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • farnesyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
  • isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
  • organic acid phosphorylation Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processIsoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR220W-MONOMER.
YEAST:YMR220W-MONOMER.
UniPathwayiUPA00057; UER00099.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomevalonate kinase (EC:2.7.4.2)
Gene namesi
Name:ERG8
Ordered Locus Names:YMR220W
ORF Names:YM9959.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR220W.
SGDiS000004833. ERG8.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001566711 – 451Phosphomevalonate kinaseAdd BLAST451

Proteomic databases

MaxQBiP24521.
PRIDEiP24521.
TopDownProteomicsiP24521.

Interactioni

Protein-protein interaction databases

BioGridi35398. 150 interactors.
MINTiMINT-4498550.
STRINGi4932.YMR220W.

Structurei

3D structure databases

ProteinModelPortaliP24521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000175372.
InParanoidiP24521.
KOiK00938.
OMAiRMCDVDC.
OrthoDBiEOG092C392D.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProiView protein in InterPro
IPR016005. Erg8.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR035102. Phosphomevalonate_kinase_2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
PANTHERiPTHR31814:SF3. PTHR31814:SF3. 1 hit.
PfamiView protein in Pfam
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
PIRSFiPIRSF017288. PMK_GHMP_euk. 1 hit.
SUPFAMiSSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR01219. Pmev_kin_ERG8. 1 hit.
PROSITEiView protein in PROSITE
PS00627. GHMP_KINASES_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P24521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELRAFSAP GKALLAGGYL VLDTKYEAFV VGLSARMHAV AHPYGSLQGS
60 70 80 90 100
DKFEVRVKSK QFKDGEWLYH ISPKSGFIPV SIGGSKNPFI EKVIANVFSY
110 120 130 140 150
FKPNMDDYCN RNLFVIDIFS DDAYHSQEDS VTEHRGNRRL SFHSHRIEEV
160 170 180 190 200
PKTGLGSSAG LVTVLTTALA SFFVSDLENN VDKYREVIHN LAQVAHCQAQ
210 220 230 240 250
GKIGSGFDVA AAAYGSIRYR RFPPALISNL PDIGSATYGS KLAHLVDEED
260 270 280 290 300
WNITIKSNHL PSGLTLWMGD IKNGSETVKL VQKVKNWYDS HMPESLKIYT
310 320 330 340 350
ELDHANSRFM DGLSKLDRLH ETHDDYSDQI FESLERNDCT CQKYPEITEV
360 370 380 390 400
RDAVATIRRS FRKITKESGA DIEPPVQTSL LDDCQTLKGV LTCLIPGAGG
410 420 430 440 450
YDAIAVITKQ DVDLRAQTAN DKRFSKVQWL DVTQADWGVR KEKDPETYLD

K
Length:451
Mass (Da):50,455
Last modified:October 1, 1996 - v2
Checksum:iDB2B6C862153683C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213A → R in AAA34596 (PubMed:1846667).Curated1
Sequence conflicti418 – 423TANDKR → PLMTKD (PubMed:1846667).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63648 Genomic DNA. Translation: AAA34596.1.
Z49939 Genomic DNA. Translation: CAA90191.1.
BK006946 Genomic DNA. Translation: DAA10119.1.
PIRiS57588.
RefSeqiNP_013947.1. NM_001182727.1.

Genome annotation databases

EnsemblFungiiYMR220W; YMR220W; YMR220W.
GeneIDi855260.
KEGGisce:YMR220W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiERG8_YEAST
AccessioniPrimary (citable) accession number: P24521
Secondary accession number(s): D6W045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names