P24504 (NUP3_PENSQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Nuclease PA3 EC=3.1.3.6 Alternative name(s): Deoxyribonuclease PA3 Endonuclease PA3
Organism	Penicillium sp.
Taxonomic identifier	5081 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes only single stranded DNA and RNA without apparent specificity for bases.
Catalytic activity	A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Binds 3 zinc ions.
Subcellular location	Secreted.
Sequence similarities	To A.oryzae nuclease S1, and barley nuclease.

Ontologies

Keywords
Cellular component	Secreted
Ligand	Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-nucleotidase activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Nuclease PA3		PRO_0000058004
Sites
	Metal binding	1	1	Zinc 3 By similarity
	Metal binding	6	1	Zinc 3 By similarity
	Metal binding	45	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	116	1	Zinc 1 By similarity
	Metal binding	120	1	Zinc 1 By similarity
	Metal binding	120	1	Zinc 3 By similarity
	Metal binding	126	1	Zinc 2 By similarity
	Metal binding	149	1	Zinc 2 By similarity
	Metal binding	153	1	Zinc 2 By similarity
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Ref.1
	Glycosylation	138	1	N-linked (GlcNAc...) Ref.1
	Glycosylation	184	1	N-linked (GlcNAc...) Ref.1
	Glycosylation	197	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	72 ↔ 217		By similarity
	Disulfide bond	80 ↔ 85		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24504 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: EF52CFDBBA4F16EF	FASTA	270	29,215
	10 20 30 40 50 60 WGALGHATVA YVAQHYVSPE AASWAQGILG SSSSSYLASI ASWADEYRLT SAGKWSASLH 70 80 90 100 110 120 FIDAEDNPPT NCNVDYERDC GSSGCSISAI ANYTQRVSDS SLSSENHAEA LRFLVHFIGD 130 140 150 160 170 180 MTQPLHDEAY AVGGNKINVT FDGYHDNLHS DWDTYMPQKL IGGHALSDAE SWAKTLVQNI 190 200 210 220 230 240 ESGNYTAQAT GWIKGDNISE PITTATRWAS DANALVCTVV MPHGAAALQT GDLYPTYYDS 250 260 270 VIDTIELQIA KGGYRLANWI NEIHGSEIAK

References

[1]	"Primary structure of a nuclease (nuclease PA3) from a Penicillium sp." Tabata N., Kazama H., Ohgi K., Irie M. Agric. Biol. Chem. 55:461-469(1991) [PubMed: 1369324] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Purification and characterization of a nuclease (3'-nucleotidase) from a Penicillium sp." Kazama H., Tabata N., Ohgi K., Irie M. Chem. Pharm. Bull. 38:3081-3085(1990) [PubMed: 1964878] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30 AND 268-270.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	JE0408.
3D structure databases
ProteinModelPortal	P24504.
SMR	P24504. Positions 1-264.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008947. PLipase_C/P1_nuclease. IPR003154. S1/P1nuclease. [Graphical view]
Gene3D	G3DSA:1.10.575.10. Phospholipase_C/P1_nuclease. 1 hit.
Pfam	PF02265. S1-P1_nuclease. 1 hit. [Graphical view]
SUPFAM	SSF48537. PLC_Nuclease. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUP3_PENSQ

Accession

Primary (citable) accession number: P24504

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	October 19, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program