P24486 (NTP1_CBEPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:

NPH1

Organism

Choristoneura biennis entomopoxvirus (CbEPV)

Taxonomic identifier

10288 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae › Betaentomopoxvirus

Virus host

Choristoneura fumiferana (Spruce budworm) [TaxID: 7141]

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Nucleoside triphosphatase I		PRO_0000099100
Regions
	Domain	48 – 213	166	Helicase ATP-binding
	Domain	379 – 542	164	Helicase C-terminal
	Nucleotide binding	61 – 68	8	ATP By similarity
	Region	468 – 534	67	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	151 – 154	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	P24486 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: C61BA6EA5D36F892	FASTA	648	76,116
	10 20 30 40 50 60 MFALDSIVGK HINYALDKTQ HLPNKINNSI TNTEITLQDY QYFASRIFIG LKNLNSMLLF 70 80 90 100 110 120 WDTGTGKTLT AVYIIKYIKE LFPRWIILIF IKKSLYIDPW LNTISSYISD TSNIKFIYYD 130 140 150 160 170 180 STSSLDKKFN NIYRSIESSL NKKNRLLIII DEVHKLISRS VKKDNNERNF TPIYRKLIKL 190 200 210 220 230 240 ANYENNKILC MSATPITNNI AEFNNLIGLL RPNVMNIKEE YINNGKLINF KEVRETLLGI 250 260 270 280 290 300 CSYKRLIEAD SLTDTNYIDG YAKKSIFYHN IIMSDEQSKL YNMAERYDYK TELGGLKTMR 310 320 330 340 350 360 RLISSFAFYD LKIKGDLDNV EYNEMIKRKL AEFSEFTKNI NFSKAFINAF KNNEIKSKTD 370 380 390 400 410 420 LLITDINNYN ILYQYSCKYI EACRIILNSR GKVLLFEPLV NFEGISSLKY YFNCFNISYI 430 440 450 460 470 480 EYSSKTIKMR DNDLNEYNNY ENNDGNKIKV CIFSYAGSEG ISFKCINDII ILDMPWNESE 490 500 510 520 530 540 LKQIIGRSIR LNSHEYLPIN YRYVNVHFII SYSNNRKSVD KEMLDIIKNK QGKINVVFDL 550 560 570 580 590 600 LKASSIETIH NMHKYIEPVD NEIIFEIIRK TRMKEMNISN VIINLKLYPI TYCKDYDRAT 610 620 630 640 ILKGFLNKDT NIIYDNDTPV AKLIVDNNNL PIFVIENDIL IYITNDYY

References

[1]	"DNA sequence of the nucleoside triphosphate phosphohydrolase I (NPH I) of the Choristoneura biennis entomopoxvirus." Yuen L., Noiseux M., Gomes M. Virology 182:403-406(1991) [PubMed: 1850911] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60400 Genomic DNA. Translation: AAA42886.1.
PIR	NPVZCP. A39154.
3D structure databases
ProteinModelPortal	P24486.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_CBEPV

Accession

Primary (citable) accession number: P24486

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program