P24485 (CD53_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leukocyte surface antigen CD53

Alternative name(s):
Cell surface glycoprotein CD53
Leukocyte antigen MRC OX-44
CD_antigen=CD53

Gene names

Name:	Cd53
Synonyms:	Ox-44

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in growth regulation in hematopoietic cells.
Subunit structure	Interacts with SCIMP By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Spleen and thymus, B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes, peripheral T-cells.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of growth Non-traceable author statement Ref.1. Source: RGD
Cellular_component	cell surface Inferred from direct assay Ref.1. Source: RGD immunological synapse Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 219	218	Leukocyte surface antigen CD53		PRO_0000219214
Regions
	Topological domain	2 – 11	10	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Potential
	Topological domain	33 – 54	22	Extracellular Potential
	Transmembrane	55 – 69	15	Helical; Potential
	Topological domain	70 – 80	11	Cytoplasmic Potential
	Transmembrane	81 – 106	26	Helical; Potential
	Topological domain	107 – 181	75	Extracellular Potential
	Transmembrane	182 – 206	25	Helical; Potential
	Topological domain	207 – 219	13	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	129	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P24485 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C73FC368311F172D	FASTA	219	24,168
	10 20 30 40 50 60 MGMSSLKLLK YVLFFFNFLF WVCGCCILGF GIHLLVQNTY GILFRNLPFL TLGNVLVIVG 70 80 90 100 110 120 SIIMVVAFLG CMGSIKENKC LLMSFFVLLL LILLAEVTLA ILLFVYEKKI NTLVAEGLND 130 140 150 160 170 180 SIQHYHSDNS TRMAWDFIQS QLQCCGVNGS SDWISGPPSS CPSGADVQGC YKKGQAWFHS 190 200 210 NFLYIGIVTI CVCVIQVLGM SFALTLNCQI DKTSQALGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The rat leukocyte antigen MRC OX-44 is a member of a new family of cell surface proteins which appear to be involved in growth regulation." Bellacosa A., Lazo P.A., Bear S.E., Tsichlis P.N. Mol. Cell. Biol. 11:2864-2872(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"The primary structure of the human leukocyte antigen CD37, a species homologue of the rat MRC OX-44 antigen." Classon B.J., Williams A.F., Willis A.C., Seed B., Stamenkovic I. J. Exp. Med. 169:1497-1502(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-30.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57276 mRNA. Translation: AAA41775.1. BC078900 mRNA. Translation: AAH78900.1.
IPI	IPI00231743.
PIR	A39574.
RefSeq	NP_036655.1. NM_012523.2.
UniGene	Rn.31988.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000024344.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000024344; ENSRNOP00000024344; ENSRNOG00000017874.
GeneID	24251.
KEGG	rno:24251.
UCSC	RGD:2310. rat.
Organism-specific databases
CTD	963.
RGD	2310. Cd53.
Phylogenomic databases
eggNOG	NOG265641.
GeneTree	ENSGT00670000097822.
HOGENOM	HOG000230651.
HOVERGEN	HBG002324.
InParanoid	P24485.
KO	K06489.
OMA	VIQVLGM.
OrthoDB	EOG4ZS94F.
Gene expression databases
Genevestigator	P24485.
GermOnline	ENSRNOG00000017874. Rattus norvegicus.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	602765.

Entry information

Entry name

CD53_RAT

Accession

Primary (citable) accession number: P24485

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program