DNA polymerase epsilon subunit B - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P24482 (DPB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA polymerase epsilon subunit B
EC=2.7.7.7

Alternative name(s):
DNA polymerase II subunit 2

Gene names

Name:	DPB2
Ordered Locus Names:	YPR175W
ORF Names:	P9705.7

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. Ref.6
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4. Ref.5
Subcellular location	Cytoplasm. Nucleus Ref.9.
Post-translational modification	Phosphorylated in a cell cycle dependent manner during late G1 phase. Phosphorylation may facilitate the interaction with POL2 or the activity of DNA polymerase II. Phosphorylation is independent of DNA replication but dependent upon CDC28 in vivo. Both Ser-141 and Ser-613 are phosphorylated in vivo, but in vitro only Ser-141 is phosphorylated by CDC28. Ref.4
Miscellaneous	Present with 3110 +/- 251 molecules/cell in log phase SD medium. In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Sequence similarities	Belongs to the DNA polymerase epsilon subunit B family.
Sequence caution	The sequence AAA34576.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB68109.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW error-prone translesion synthesis Inferred from direct assay PubMed 19776424. Source: SGD heterochromatin organization involved in chromatin silencing Inferred by curator PubMed 14673157. Source: SGD maintenance of fidelity involved in DNA-dependent DNA replication Inferred from mutant phenotype PubMed 18245343 PubMed 19463834. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 15282802. Source: SGD epsilon DNA polymerase complex Inferred from direct assay Ref.7 PubMed 14673157 PubMed 2406268. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 689

689

DNA polymerase epsilon subunit B

PRO_0000071573

Amino acid modifications

Modified residue

141

Phosphoserine; by CDC28 Ref.4

Modified residue

613

Phosphoserine Ref.4

Experimental info

Sequence conflict

458

F → Y in AAA34576. Ref.1

Sequence conflict

521

K → R in AAA34576. Ref.1

Sequence conflict

565

V → F in AAA34576. Ref.1

Sequence conflict

584

E → Q in AAA34576. Ref.1

Sequence conflict

644

T → I in AAA34576. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P24482 [UniParc].

Last modified January 10, 2006. Version 3.
Checksum: 0936E3FC90552788
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FASTA

689

78,340

        10         20         30         40         50         60 
MFGSGNVLPV KIQPPLLRPL AYRVLSRKYG LSIKSDGLSA LAEFVGTNIG ANWRQGPATI 

        70         80         90        100        110        120 
KFLEQFAAVW KQQERGLFID QSGVKEVIQE MKEREKVEWS HEHPIQHEEN ILGRTDDDEN 

       130        140        150        160        170        180 
NSDDEMPIAA DSSLQNVSLS SPMRQPTERD EYKQPFKPES SKALDWRDYF KVINASQQQR 

       190        200        210        220        230        240 
FSYNPHKMQF IFVPNKKQNG LGGIAGFLPD IEDKVQMFLT RYYLTNDRVM RNENFQNSDM 

       250        260        270        280        290        300 
FNPLSSMVSL QNELSNTNRQ QQSSSMSITP IKNLLGRDAQ NFLLLGLLNK NFKGNWSLED 

       310        320        330        340        350        360 
PSGSVEIDIS QTIPTQGHYY VPGCMVLVEG IYYSVGNKFH VTSMTLPPGE RREITLETIG 

       370        380        390        400        410        420 
NLDLLGIHGI SNNNFIARLD KDLKIRLHLL EKELTDHKFV ILGANLFLDD LKIMTALSKI 

       430        440        450        460        470        480 
LQKLNDDPPT LLIWQGSFTS VPVFASMSSR NISSSTQFKN NFDALATLLS RFDNLTENTT 

       490        500        510        520        530        540 
MIFIPGPNDL WGSMVSLGAS GTLPQDPIPS AFTKKINKVC KNVVWSSNPT RIAYLSQEIV 

       550        560        570        580        590        600 
IFRDDLSGRF KRHRLEFPFN ESEDVYTEND NMMSKDTDIV PIDELVKEPD QLPQKVQETR 

       610        620        630        640        650        660 
KLVKTILDQG HLSPFLDSLR PISWDLDHTL TLCPIPSTMV LCDTTSAQFD LTYNGCKVIN 

       670        680 
PGSFIHNRRA RYMEYVPSSK KTIQEEIYI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae." Araki H., Hamatake R.K., Johnston L.H., Sugino A. Proc. Natl. Acad. Sci. U.S.A. 88:4601-4605(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YHA8.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Cell cycle-dependent phosphorylation of the DNA polymerase epsilon subunit, Dpb2, by the Cdc28 cyclin-dependent protein kinase." Kesti T., McDonald W.H., Yates J.R. III, Wittenberg C. J. Biol. Chem. 279:14245-14255(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 132-144 AND 606-622, PHOSPHORYLATION AT SER-141 AND SER-613, MASS SPECTROMETRY.
[5]	"Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae." Ohya T., Maki S., Kawasaki Y., Sugino A. Nucleic Acids Res. 28:3846-3852(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[6]	"Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae." Shimizu K., Hashimoto K., Kirchner J.M., Nakai W., Nishikawa H., Resnick M.A., Sugino A. J. Biol. Chem. 277:37422-37429(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"The quaternary structure of DNA polymerase epsilon from Saccharomyces cerevisiae." Chilkova O., Jonsson B.-H., Johansson E. J. Biol. Chem. 278:14082-14086(2003) [PubMed] [Europe PMC] [Abstract] Cited for: COMPOSITION OF THE DNA POLYMERASE EPSILON COMPLEX.
[8]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M61710 Genomic DNA. Translation: AAA34576.1. Different initiation. U25842 Genomic DNA. Translation: AAB68109.1. Different initiation. BK006949 Genomic DNA. Translation: DAA11592.1.
PIR	S59833.
RefSeq	NP_015501.2. NM_001184272.1.
3D structure databases
ProteinModelPortal	P24482.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2533N.
IntAct	P24482. 23 interactions.
MINT	MINT-532205.
STRING	4932.YPR175W.
Proteomic databases
PaxDb	P24482.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR175W; YPR175W; YPR175W.
GeneID	856305.
KEGG	sce:YPR175W.
Organism-specific databases
CYGD	YPR175w.
SGD	S000006379. DPB2.
Phylogenomic databases
eggNOG	NOG311342.
GeneTree	ENSGT00390000012435.
HOGENOM	HOG000246706.
KO	K02325.
OMA	GSIEYKE.
OrthoDB	EOG4T4H3M.
Gene expression databases
Genevestigator	P24482.
GermOnline	YPR175W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007185. DNA_pol_alpha/epsilon_bsu. [Graphical view]
Pfam	PF04042. DNA_pol_E_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981670.

Entry information

Entry name

DPB2_YEAST

Accession

Primary (citable) accession number: P24482
Secondary accession number(s): D6W4H6, Q06622

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 10, 2006
Last modified:	May 1, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents