P24482 (DPB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase epsilon subunit B
EC=2.7.7.7

Alternative name(s):
DNA polymerase II subunit 2

Gene names

Name:	DPB2
Ordered Locus Names:	YPR175W
ORF Names:	P9705.7

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. Ref.6
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4. Ref.5
Subcellular location	Cytoplasm. Nucleus Ref.9.
Post-translational modification	Phosphorylated in a cell cycle dependent manner during late G1 phase. Phosphorylation may facilitate the interaction with POL2 or the activity of DNA polymerase II. Phosphorylation is independent of DNA replication but dependent upon CDC28 in vivo. Both Ser-141 and Ser-613 are phosphorylated in vivo, but in vitro only Ser-141 is phosphorylated by CDC28. Ref.4
Miscellaneous	Present with 3110 +/- 251 molecules/cell in log phase SD medium. Ref.10 In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Sequence similarities	Belongs to the DNA polymerase epsilon subunit B family.
Sequence caution	The sequence AAA34576.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB68109.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW lagging strand elongation Traceable author statement. Source: SGD leading strand elongation Traceable author statement. Source: SGD mismatch repair Traceable author statement. Source: SGD nucleotide-excision repair Traceable author statement. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD epsilon DNA polymerase complex Inferred from direct assay. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	DNA polymerase epsilon subunit B		PRO_0000071573
Amino acid modifications
	Modified residue	141	1	Phosphoserine; by CDC28 Ref.4
	Modified residue	613	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	458	1	F → Y in AAA34576. Ref.1
	Sequence conflict	521	1	K → R in AAA34576. Ref.1
	Sequence conflict	565	1	V → F in AAA34576. Ref.1
	Sequence conflict	584	1	E → Q in AAA34576. Ref.1
	Sequence conflict	644	1	T → I in AAA34576. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24482 [UniParc]. Last modified January 10, 2006. Version 3. Checksum: 0936E3FC90552788	FASTA	689	78,340
	10 20 30 40 50 60 MFGSGNVLPV KIQPPLLRPL AYRVLSRKYG LSIKSDGLSA LAEFVGTNIG ANWRQGPATI 70 80 90 100 110 120 KFLEQFAAVW KQQERGLFID QSGVKEVIQE MKEREKVEWS HEHPIQHEEN ILGRTDDDEN 130 140 150 160 170 180 NSDDEMPIAA DSSLQNVSLS SPMRQPTERD EYKQPFKPES SKALDWRDYF KVINASQQQR 190 200 210 220 230 240 FSYNPHKMQF IFVPNKKQNG LGGIAGFLPD IEDKVQMFLT RYYLTNDRVM RNENFQNSDM 250 260 270 280 290 300 FNPLSSMVSL QNELSNTNRQ QQSSSMSITP IKNLLGRDAQ NFLLLGLLNK NFKGNWSLED 310 320 330 340 350 360 PSGSVEIDIS QTIPTQGHYY VPGCMVLVEG IYYSVGNKFH VTSMTLPPGE RREITLETIG 370 380 390 400 410 420 NLDLLGIHGI SNNNFIARLD KDLKIRLHLL EKELTDHKFV ILGANLFLDD LKIMTALSKI 430 440 450 460 470 480 LQKLNDDPPT LLIWQGSFTS VPVFASMSSR NISSSTQFKN NFDALATLLS RFDNLTENTT 490 500 510 520 530 540 MIFIPGPNDL WGSMVSLGAS GTLPQDPIPS AFTKKINKVC KNVVWSSNPT RIAYLSQEIV 550 560 570 580 590 600 IFRDDLSGRF KRHRLEFPFN ESEDVYTEND NMMSKDTDIV PIDELVKEPD QLPQKVQETR 610 620 630 640 650 660 KLVKTILDQG HLSPFLDSLR PISWDLDHTL TLCPIPSTMV LCDTTSAQFD LTYNGCKVIN 670 680 PGSFIHNRRA RYMEYVPSSK KTIQEEIYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae." Araki H., Hamatake R.K., Johnston L.H., Sugino A. Proc. Natl. Acad. Sci. U.S.A. 88:4601-4605(1991) [PubMed: 2052544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YHA8.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Cell cycle-dependent phosphorylation of the DNA polymerase epsilon subunit, Dpb2, by the Cdc28 cyclin-dependent protein kinase." Kesti T., McDonald W.H., Yates J.R. III, Wittenberg C. J. Biol. Chem. 279:14245-14255(2004) [PubMed: 14747467] [Abstract] Cited for: PROTEIN SEQUENCE OF 132-144 AND 606-622, PHOSPHORYLATION AT SER-141 AND SER-613, MASS SPECTROMETRY.
[5]	"Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae." Ohya T., Maki S., Kawasaki Y., Sugino A. Nucleic Acids Res. 28:3846-3852(2000) [PubMed: 11024162] [Abstract] Cited for: SUBUNIT.
[6]	"Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae." Shimizu K., Hashimoto K., Kirchner J.M., Nakai W., Nishikawa H., Resnick M.A., Sugino A. J. Biol. Chem. 277:37422-37429(2002) [PubMed: 12124389] [Abstract] Cited for: FUNCTION.
[7]	"The quaternary structure of DNA polymerase epsilon from Saccharomyces cerevisiae." Chilkova O., Jonsson B.-H., Johansson E. J. Biol. Chem. 278:14082-14086(2003) [PubMed: 12571237] [Abstract] Cited for: COMPOSITION OF THE DNA POLYMERASE EPSILON COMPLEX.
[8]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M61710 Genomic DNA. Translation: AAA34576.1. Different initiation. U25842 Genomic DNA. Translation: AAB68109.1. Different initiation. BK006949 Genomic DNA. Translation: DAA11592.1.
PIR	S59833.
RefSeq	NP_015501.2. NM_001184272.1.
3D structure databases
ProteinModelPortal	P24482.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2533N.
IntAct	P24482. 25 interactions.
MINT	MINT-532205.
STRING	P24482.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR175W; YPR175W; YPR175W.
GeneID	856305.
KEGG	sce:YPR175W.
NMPDR	fig|4932.3.peg.6650.
Organism-specific databases
CYGD	YPR175w.
SGD	S000006379. DPB2.
Phylogenomic databases
eggNOG	fuNOG04205.
GeneTree	EFGT00050000005199.
HOGENOM	HBG398250.
OMA	GSIEYKE.
OrthoDB	EOG4T4H3M.
Gene expression databases
ArrayExpress	P24482.
Genevestigator	P24482.
GermOnline	YPR175W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007185. DNA_pol_alpha/epsilon_bsu. [Graphical view]
KO	K02325.
Pfam	PF04042. DNA_pol_E_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981670.

Entry information

Entry name

DPB2_YEAST

Accession

Primary (citable) accession number: P24482
Secondary accession number(s): D6W4H6, Q06622

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 10, 2006
Last modified:	December 14, 2011
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program