Reviewed, UniProtKB/Swiss-Prot P24478 (RIPS_TRIKI)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein karasurin-C     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase Cleaved into the following chain:     1- Recommended name:             Ribosome-inactivating protein karasurin-A
Organism	Trichosanthes kirilowii (Mongolian snake-gourd)
Taxonomic identifier	3677 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Trichosanthes

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Abortion-inducing protein. It inactivates eukaryotic 60S ribosomal subunits.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2
	Chain	22 – 270	249	Ribosome-inactivating protein karasurin-C		PRO_0000030767
	Chain	24 – 270	247	Ribosome-inactivating protein karasurin-A		PRO_0000030768
	Propeptide	271 – 289	19	Removed in mature form		PRO_0000030769
Sites
	Active site	183	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24478-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 883D3E3242887B26	FASTA	289	31,704
	10 20 30 40 50 60 MIRFLVFSLL ILTLFLTAPA VEGDVSFRLS GATSSSYGVF ISNLRKALPY ERKLYDIPLL 70 80 90 100 110 120 RSTLPGSQRY ALIHLTNYAD ETISVAIDVT NVYVMGYRAG DTSYFFNEAS ATEAAKYVFK 130 140 150 160 170 180 DAKRKVTLPY SGNYERLQIA AGKIRENIPL GLPALDSAIT TLFYYNANSA ASALMVLIQS 190 200 210 220 230 240 TSEAARYKFI EQQIGKRVDK TFLPSLAIIS LENSWSALSK QIQIASTNNG QFETPVVLIN 250 260 270 280 AQNQRVTITN VDAGVVTSNI ALLLNRNNMA AIDDDVPMAQ SFGCGSYAI

References

[1]	"Cloning and bacterial expression of a gene encoding ribosome-inactivating proteins, karasurin-A and karasurin-C, from Trichosanthes kirilowii var. japonica." Mizukami H., Iida K., Kondo T., Ogihara Y. Biol. Pharm. Bull. 20:711-713(1997) [PubMed: 9212998] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Root tuber.
[2]	"Amino acid sequences and ribosome-inactivating activities of karasurin-B and karasurin-C." Kondo T., Mizukami H., Takeda T., Ogihara Y. Biol. Pharm. Bull. 19:1485-1489(1996) [PubMed: 8951169] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-270.
[3]	"The complete amino acid sequence of an abortifacient protein, karasurin." Toyokawa S., Takeda T., Kato Y., Wakabayashi K., Ogihara Y. Chem. Pharm. Bull. 39:1244-1249(1991) [PubMed: 1914000] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-270.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB000666 Genomic DNA. Translation: BAA21786.1.
PIR	JC5606. JU0393.
3D structure databases
HSSP	HSSP built from PDB template 1MRJ based on UniProtKB P09989.
SMR	P24478. Positions 24-270.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273574.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIPS_TRIKI

Accession

Primary (citable) accession number: P24478

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)