Reviewed, UniProtKB/Swiss-Prot P24477 (RIP2_DIACA)

Names and origin

Protein names	Recommended name:     Antiviral protein DAP-32     EC=3.2.2.22 Alternative name(s):     Ribosome-inactivating protein     rRNA N-glycosidase
Organism	Dianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifier	3570 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Dianthus

Protein attributes

Sequence length	60 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Single-chain ribosome-inactivating protein, possessing high antiviral potency and low toxicity to normal cells in culture and to intact animals. Capable of inhibiting HIV-1 infection and replication.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›60	›60	Antiviral protein DAP-32		PRO_0000221419
Experimental info
	Non-terminal residue	60	1

Sequences

	Sequence		Length	Mass (Da)
	P24477-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 0F8EF1A1395CAD90	FASTA	60	6,602
	10 20 30 40 50 60 AVKTITLNLV SPSANRYATF LTEIRDNVRX RSLDYSHSGI DVIGAPSSRD SXLNINFQSP

References

[1]	"A new class of anti-HIV agents: GAP31, DAPs 30 and 32." Lee-Huang S., Kung H.-F., Huang P.L., Huang P.L., Li B.-Q., Huang P., Huang H.I., Chen H.-C. FEBS Lett. 291:139-144(1991) [PubMed: 1936243] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Leaf.

Cross-references

Sequence databases
PIR	S17686.
3D structure databases
HSSP	HSSP built from PDB template 1QI7 based on UniProtKB P27559.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 21500.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. [Graphical view]
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP2_DIACA

Accession

Primary (citable) accession number: P24477

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)