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Protein

Antiviral protein DAP-30

Gene
N/A
Organism
Dianthus caryophyllus (Carnation) (Clove pink)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Single-chain ribosome-inactivating protein.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei200By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Hydrolase, Protein synthesis inhibitor, Toxin

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral protein DAP-30 (EC:3.2.2.22)
Alternative name(s):
Dianthin 30
Ribosome-inactivating protein
rRNA N-glycosidase
OrganismiDianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifieri3570 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeCaryophylleaeDianthus

Pathology & Biotechi

Protein family/group databases

Allergomei2796. Dia c RIP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000003078924 – 293Antiviral protein DAP-30Add BLAST270

Proteomic databases

PRIDEiP24476.

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 30Combined sources6
Helixi36 – 50Combined sources15
Beta strandi63 – 65Combined sources3
Beta strandi70 – 72Combined sources3
Beta strandi74 – 81Combined sources8
Beta strandi84 – 91Combined sources8
Turni92 – 94Combined sources3
Beta strandi97 – 103Combined sources7
Beta strandi109 – 113Combined sources5
Turni115 – 117Combined sources3
Helixi120 – 126Combined sources7
Helixi132 – 134Combined sources3
Beta strandi135 – 137Combined sources3
Helixi144 – 150Combined sources7
Helixi160 – 163Combined sources4
Helixi167 – 174Combined sources8
Turni175 – 179Combined sources5
Helixi184 – 197Combined sources14
Helixi199 – 203Combined sources5
Helixi205 – 213Combined sources9
Turni214 – 216Combined sources3
Helixi223 – 230Combined sources8
Helixi232 – 241Combined sources10
Beta strandi242 – 244Combined sources3
Beta strandi247 – 256Combined sources10
Beta strandi258 – 261Combined sources4
Helixi262 – 265Combined sources4
Beta strandi267 – 270Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LP8X-ray1.65A24-277[»]
1LPCX-ray1.70A24-277[»]
1LPDX-ray1.70A24-277[»]
1RL0X-ray1.40A24-278[»]
ProteinModelPortaliP24476.
SMRiP24476.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24476.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIYLVAAIA WILFQSSSWT TDAATAYTLN LANPSASQYS SFLDQIRNNV
60 70 80 90 100
RDTSLIYGGT DVAVIGAPST TDKFLRLNFQ GPRGTVSLGL RRENLYVVAY
110 120 130 140 150
LAMDNANVNR AYYFKNQITS AELTALFPEV VVANQKQLEY GEDYQAIEKN
160 170 180 190 200
AKITTGDQSR KELGLGINLL ITMIDGVNKK VRVVKDEARF LLIAIQMTAE
210 220 230 240 250
AARFRYIQNL VTKNFPNKFD SENKVIQFQV SWSKISTAIF GDCKNGVFNK
260 270 280 290
DYDFGFGKVR QAKDLQMGLL KYLGRPKSSS IEANSTDDTA DVL
Length:293
Mass (Da):32,717
Last modified:December 1, 1992 - v2
Checksum:iAC85AAAC8725D4F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59260 mRNA. Translation: CAA41953.1. Sequence problems.
PIRiS17519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59260 mRNA. Translation: CAA41953.1. Sequence problems.
PIRiS17519.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LP8X-ray1.65A24-277[»]
1LPCX-ray1.70A24-277[»]
1LPDX-ray1.70A24-277[»]
1RL0X-ray1.40A24-278[»]
ProteinModelPortaliP24476.
SMRiP24476.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei2796. Dia c RIP.

Proteomic databases

PRIDEiP24476.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP24476.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIP0_DIACA
AccessioniPrimary (citable) accession number: P24476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibits HIV-1 infection and replication.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.