Reviewed, UniProtKB/Swiss-Prot P24476 (RIP0_DIACA)

Names and origin

Protein names	Recommended name:     Antiviral protein DAP-30     EC=3.2.2.22 Alternative name(s):     Ribosome-inactivating protein     rRNA N-glycosidase     Dianthin 30
Organism	Dianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifier	3570 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Dianthus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Single-chain ribosome-inactivating protein.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Miscellaneous	Inhibits HIV-1 infection and replication.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Antiviral protein Hydrolase Protein synthesis inhibitor Toxin
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.2

Chain

24 – 293

270

Antiviral protein DAP-30

PRO_0000030789

Sites

Active site

200

1

By similarity

Secondary structure

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293

Helix Strand Turn

Details...

Beta strand

25 – 30

6

Helix

36 – 50

15

Beta strand

70 – 72

3

Beta strand

74 – 81

8

Beta strand

84 – 91

8

Turn

92 – 94

3

Beta strand

97 – 103

7

Beta strand

109 – 113

5

Turn

115 – 117

3

Helix

120 – 126

7

Helix

132 – 134

3

Beta strand

135 – 137

3

Helix

144 – 150

7

Helix

160 – 163

4

Helix

167 – 174

8

Turn

175 – 179

5

Helix

184 – 197

14

Helix

199 – 203

5

Helix

205 – 213

9

Turn

214 – 216

3

Helix

223 – 230

8

Helix

232 – 241

10

Beta strand

242 – 244

3

Beta strand

247 – 256

10

Beta strand

258 – 261

4

Helix

262 – 265

4

Sequences

	Sequence		Length	Mass (Da)
	P24476-1 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: AC85AAAC8725D4F2	FASTA	293	32,717
	10 20 30 40 50 60 MKIYLVAAIA WILFQSSSWT TDAATAYTLN LANPSASQYS SFLDQIRNNV RDTSLIYGGT 70 80 90 100 110 120 DVAVIGAPST TDKFLRLNFQ GPRGTVSLGL RRENLYVVAY LAMDNANVNR AYYFKNQITS 130 140 150 160 170 180 AELTALFPEV VVANQKQLEY GEDYQAIEKN AKITTGDQSR KELGLGINLL ITMIDGVNKK 190 200 210 220 230 240 VRVVKDEARF LLIAIQMTAE AARFRYIQNL VTKNFPNKFD SENKVIQFQV SWSKISTAIF 250 260 270 280 290 GDCKNGVFNK DYDFGFGKVR QAKDLQMGLL KYLGRPKSSS IEANSTDDTA DVL

References

[1]	"Nucleotide sequence of cDNA coding for dianthin 30, a ribosome inactivating protein from Dianthus caryophyllus." Legname G., Bellosta P., Gromo G., Modena D., Keen J.N., Roberts L.M., Lord J.M. Biochim. Biophys. Acta 1090:119-122(1991) [PubMed: 1840496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A new class of anti-HIV agents: GAP31, DAPs 30 and 32." Lee-Huang S., Kung H.-F., Huang P.L., Huang P.L., Li B.-Q., Huang P., Huang H.I., Chen H.-C. FEBS Lett. 291:139-144(1991) [PubMed: 1936243] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-82. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X59260 mRNA. Translation: CAA41953.1. Sequence problems.

PIR

S17519.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LP8	X-ray	1.65	A	24-277	[»]
1LPC	X-ray	1.70	A	24-277	[»]
1LPD	X-ray	1.70	A	24-277	[»]
1RL0	X-ray	1.40	A	24-278	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.2.2.22. 21500.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00173. Adenine.

Entry information

Entry name

RIP0_DIACA

Accession

Primary (citable) accession number: P24476

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)