Reviewed, UniProtKB/Swiss-Prot P24475 (RIP3_GELMU)

Names and origin

Protein names	Recommended name:     Antiviral protein GAP-31     EC=3.2.2.22 Alternative name(s):     Ribosome-inactivating protein     rRNA N-glycosidase
Organism	Gelonium multiflorum (Euphorbiaceae himalaya)
Taxonomic identifier	3979 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Euphorbiaceae › Crotonoideae › Gelonieae › Gelonium

Protein attributes

Sequence length	55 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Single-chain ribosome-inactivating protein, possessing high antiviral potency and low toxicity to normal cells in culture and to intact animals. Capable of inhibiting HIV-1 infection and replication.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›55	›55	Antiviral protein GAP-31		PRO_0000221418
Experimental info
	Non-terminal residue	55	1

Sequences

	Sequence		Length	Mass (Da)
	P24475-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: ADA121C22834B5BF	FASTA	55	5,847
	10 20 30 40 50 GLDTVSFSTK GATYITYVNF LNELRVKTKP EGNSHGIPSL RKSSDDPGSS FVVAG

References

[1]	"A new class of anti-HIV agents: GAP31, DAPs 30 and 32." Lee-Huang S., Kung H.-F., Huang P.L., Huang P.L., Li B.-Q., Huang P., Huang H.I., Chen H.-C. FEBS Lett. 291:139-144(1991) [PubMed: 1936243] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed.

Cross-references

Sequence databases
PIR	S17574.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273556.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. [Graphical view]
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP3_GELMU

Accession

Primary (citable) accession number: P24475

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)