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Protein

Nitrite reductase

Gene

nirS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.
NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.

Cofactori

hemeNote: Binds 2 heme groups per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72Heme (covalent)1
Binding sitei75Heme (covalent)1
Metal bindingi76Iron (heme axial ligand)1
Metal bindingi113Iron (heme axial ligand)1
Metal bindingi207Iron (heme D1 proximal ligand)1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.7.2.1. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase (EC:1.7.2.1)
Alternative name(s):
Cytochrome cd1
Cytochrome oxidase
Hydroxylamine reductase (EC:1.7.99.1)
Gene namesi
Name:nirS
Ordered Locus Names:PA0519
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0519.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000000657626 – 568Nitrite reductaseAdd BLAST543

Proteomic databases

PaxDbiP24474.
PRIDEiP24474.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi208964.PA0519.

Structurei

Secondary structure

1568
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 35Combined sources4
Helixi44 – 46Combined sources3
Beta strandi51 – 53Combined sources3
Helixi58 – 71Combined sources14
Helixi73 – 76Combined sources4
Turni77 – 80Combined sources4
Beta strandi83 – 86Combined sources4
Helixi90 – 96Combined sources7
Helixi98 – 107Combined sources10
Beta strandi110 – 112Combined sources3
Turni117 – 120Combined sources4
Helixi124 – 133Combined sources10
Beta strandi134 – 136Combined sources3
Helixi146 – 152Combined sources7
Beta strandi154 – 157Combined sources4
Helixi159 – 161Combined sources3
Helixi172 – 174Combined sources3
Beta strandi175 – 180Combined sources6
Helixi181 – 183Combined sources3
Beta strandi185 – 190Combined sources6
Turni191 – 193Combined sources3
Beta strandi196 – 201Combined sources6
Beta strandi206 – 211Combined sources6
Beta strandi217 – 222Combined sources6
Beta strandi225 – 231Combined sources7
Beta strandi234 – 236Combined sources3
Beta strandi238 – 244Combined sources7
Beta strandi247 – 254Combined sources8
Turni261 – 263Combined sources3
Beta strandi264 – 279Combined sources16
Turni280 – 282Combined sources3
Beta strandi285 – 290Combined sources6
Beta strandi296 – 298Combined sources3
Beta strandi301 – 303Combined sources3
Beta strandi307 – 312Combined sources6
Beta strandi314 – 323Combined sources10
Turni324 – 327Combined sources4
Beta strandi328 – 333Combined sources6
Beta strandi337 – 339Combined sources3
Beta strandi341 – 346Combined sources6
Beta strandi354 – 356Combined sources3
Beta strandi362 – 367Combined sources6
Helixi368 – 370Combined sources3
Beta strandi372 – 377Combined sources6
Turni378 – 381Combined sources4
Beta strandi382 – 388Combined sources7
Beta strandi390 – 393Combined sources4
Beta strandi399 – 403Combined sources5
Turni404 – 406Combined sources3
Beta strandi407 – 424Combined sources18
Turni427 – 429Combined sources3
Turni431 – 433Combined sources3
Beta strandi436 – 442Combined sources7
Beta strandi444 – 446Combined sources3
Beta strandi459 – 462Combined sources4
Helixi470 – 473Combined sources4
Beta strandi476 – 480Combined sources5
Beta strandi489 – 491Combined sources3
Helixi493 – 497Combined sources5
Beta strandi505 – 511Combined sources7
Beta strandi513 – 523Combined sources11
Beta strandi526 – 528Combined sources3
Beta strandi531 – 536Combined sources6
Turni537 – 540Combined sources4
Beta strandi541 – 546Combined sources6
Beta strandi553 – 559Combined sources7
Helixi560 – 564Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BL9X-ray2.90A/B26-568[»]
1GJQX-ray2.70A/B26-568[»]
1HZUX-ray2.70A26-568[»]
1HZVX-ray2.83A26-568[»]
1N15X-ray2.90A/B26-568[»]
1N50X-ray2.90A/B26-568[»]
1N90X-ray2.90A/B26-568[»]
1NIRX-ray2.15A/B26-568[»]
1NNOX-ray2.65A/B26-568[»]
ProteinModelPortaliP24474.
SMRiP24474.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24474.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 140Cytochrome cPROSITE-ProRule annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 54N-terminal tailAdd BLAST29
Regioni141 – 568D1-heme domainAdd BLAST428

Sequence similaritiesi

Contains 1 cytochrome c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F64. Bacteria.
COG2010. LUCA.
HOGENOMiHOG000225986.
InParanoidiP24474.
KOiK15864.
OMAiMPNWGTS.
PhylomeDBiP24474.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR003143. Cyt_cd1_C.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 1 hit.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF51004. SSF51004. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24474-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFGKPLVGT LLASLTLLGL ATAHAKDDMK AAEQYQGAAS AVDPAHVVRT
60 70 80 90 100
NGAPDMSESE FNEAKQIYFQ RCAGCHGVLR KGATGKPLTP DITQQRGQQY
110 120 130 140 150
LEALITYGTP LGMPNWGSSG ELSKEQITLM AKYIQHTPPQ PPEWGMPEMR
160 170 180 190 200
ESWKVLVKPE DRPKKQLNDL DLPNLFSVTL RDAGQIALVD GDSKKIVKVI
210 220 230 240 250
DTGYAVHISR MSASGRYLLV IGRDARIDMI DLWAKEPTKV AEIKIGIEAR
260 270 280 290 300
SVESSKFKGY EDRYTIAGAY WPPQFAIMDG ETLEPKQIVS TRGMTVDTQT
310 320 330 340 350
YHPEPRVAAI IASHEHPEFI VNVKETGKVL LVNYKDIDNL TVTSIGAAPF
360 370 380 390 400
LHDGGWDSSH RYFMTAANNS NKVAVIDSKD RRLSALVDVG KTPHPGRGAN
410 420 430 440 450
FVHPKYGPVW STSHLGDGSI SLIGTDPKNH PQYAWKKVAE LQGQGGGSLF
460 470 480 490 500
IKTHPKSSHL YVDTTFNPDA RISQSVAVFD LKNLDAKYQV LPIAEWADLG
510 520 530 540 550
EGAKRVVQPE YNKRGDEVWF SVWNGKNDSS ALVVVDDKTL KLKAVVKDPR
560
LITPTGKFNV YNTQHDVY
Length:568
Mass (Da):62,653
Last modified:March 1, 1992 - v1
Checksum:i20FAE9FFE9127D62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16452 Genomic DNA. Translation: CAA34471.1.
AE004091 Genomic DNA. Translation: AAG03908.1.
X51631 Genomic DNA. Translation: CAA35957.1.
X51319 Genomic DNA. Translation: CAA35702.1.
PIRiA32975. OSPSA.
RefSeqiNP_249210.1. NC_002516.2.
WP_003111532.1. NZ_ASJY01000100.1.

Genome annotation databases

EnsemblBacteriaiAAG03908; AAG03908; PA0519.
GeneIDi882217.
KEGGipae:PA0519.
PATRICi19835296. VBIPseAer58763_0549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16452 Genomic DNA. Translation: CAA34471.1.
AE004091 Genomic DNA. Translation: AAG03908.1.
X51631 Genomic DNA. Translation: CAA35957.1.
X51319 Genomic DNA. Translation: CAA35702.1.
PIRiA32975. OSPSA.
RefSeqiNP_249210.1. NC_002516.2.
WP_003111532.1. NZ_ASJY01000100.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BL9X-ray2.90A/B26-568[»]
1GJQX-ray2.70A/B26-568[»]
1HZUX-ray2.70A26-568[»]
1HZVX-ray2.83A26-568[»]
1N15X-ray2.90A/B26-568[»]
1N50X-ray2.90A/B26-568[»]
1N90X-ray2.90A/B26-568[»]
1NIRX-ray2.15A/B26-568[»]
1NNOX-ray2.65A/B26-568[»]
ProteinModelPortaliP24474.
SMRiP24474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0519.

Proteomic databases

PaxDbiP24474.
PRIDEiP24474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03908; AAG03908; PA0519.
GeneIDi882217.
KEGGipae:PA0519.
PATRICi19835296. VBIPseAer58763_0549.

Organism-specific databases

PseudoCAPiPA0519.

Phylogenomic databases

eggNOGiENOG4105F64. Bacteria.
COG2010. LUCA.
HOGENOMiHOG000225986.
InParanoidiP24474.
KOiK15864.
OMAiMPNWGTS.
PhylomeDBiP24474.

Enzyme and pathway databases

BRENDAi1.7.2.1. 5087.

Miscellaneous databases

EvolutionaryTraceiP24474.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR003143. Cyt_cd1_C.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 1 hit.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF51004. SSF51004. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIRS_PSEAE
AccessioniPrimary (citable) accession number: P24474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.