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P24474 (NIRS_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrite reductase
EC=1.7.2.1
Alternative name(s):
Cytochrome cd1
Cytochrome oxidase
Hydroxylamine reductase
EC=1.7.99.1
Gene names
Name:nirS
Ordered Locus Names:PA0519
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.

NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.

Cofactor

Binds 2 heme groups per subunit.

Subunit structure

Homodimer.

Subcellular location

Periplasm.

Sequence similarities

Contains 1 cytochrome c domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.1
Chain26 – 568543Nitrite reductase
PRO_0000006576

Regions

Domain55 – 14086Cytochrome c
Region26 – 5429N-terminal tail
Region141 – 568428D1-heme domain

Sites

Metal binding761Iron (heme axial ligand)
Metal binding1131Iron (heme axial ligand)
Metal binding2071Iron (heme D1 proximal ligand)
Binding site721Heme (covalent)
Binding site751Heme (covalent)

Secondary structure

1 ...................................................................................................................... 568
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24474 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 20FAE9FFE9127D62

FASTA56862,653
        10         20         30         40         50         60 
MPFGKPLVGT LLASLTLLGL ATAHAKDDMK AAEQYQGAAS AVDPAHVVRT NGAPDMSESE 

        70         80         90        100        110        120 
FNEAKQIYFQ RCAGCHGVLR KGATGKPLTP DITQQRGQQY LEALITYGTP LGMPNWGSSG 

       130        140        150        160        170        180 
ELSKEQITLM AKYIQHTPPQ PPEWGMPEMR ESWKVLVKPE DRPKKQLNDL DLPNLFSVTL 

       190        200        210        220        230        240 
RDAGQIALVD GDSKKIVKVI DTGYAVHISR MSASGRYLLV IGRDARIDMI DLWAKEPTKV 

       250        260        270        280        290        300 
AEIKIGIEAR SVESSKFKGY EDRYTIAGAY WPPQFAIMDG ETLEPKQIVS TRGMTVDTQT 

       310        320        330        340        350        360 
YHPEPRVAAI IASHEHPEFI VNVKETGKVL LVNYKDIDNL TVTSIGAAPF LHDGGWDSSH 

       370        380        390        400        410        420 
RYFMTAANNS NKVAVIDSKD RRLSALVDVG KTPHPGRGAN FVHPKYGPVW STSHLGDGSI 

       430        440        450        460        470        480 
SLIGTDPKNH PQYAWKKVAE LQGQGGGSLF IKTHPKSSHL YVDTTFNPDA RISQSVAVFD 

       490        500        510        520        530        540 
LKNLDAKYQV LPIAEWADLG EGAKRVVQPE YNKRGDEVWF SVWNGKNDSS ALVVVDDKTL 

       550        560 
KLKAVVKDPR LITPTGKFNV YNTQHDVY

« Hide

References

« Hide 'large scale' references
[1]"Nitrite reductase from Pseudomonas aeruginosa: sequence of the gene and the protein."
Silvestrini M.C., Galeotti C.L., Gervais M., Schinina E., Barra D., Bossa F., Brunori M.
FEBS Lett. 254:33-38(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-70.
Strain: NTCC 6750.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]"Cloning and sequencing of the gene encoding cytochrome c-551 from Pseudomonas aeruginosa."
Arai H., Sanbongi Y., Igarashi Y., Kodama T.
FEBS Lett. 261:196-198(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 462-568.
[4]"The structural gene for cytochrome c551 from Pseudomonas aeruginosa. The nucleotide sequence shows a location downstream of the nitrite reductase gene."
Nordling M., Young S., Karlsson B.G., Lundberg L.G.
FEBS Lett. 259:230-232(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 462-568.
Strain: ATCC 10145 / DSM 50071 / JCM 5962 / LMG 1242 / NBRC 12689 / NCIMB 8295 / NRRL B-771.
[5]"N-terminal arm exchange is observed in the 2.15 A crystal structure of oxidized nitrite reductase from Pseudomonas aeruginosa."
Nurizzo D., Silvestrini M.-C., Mathieu M., Cutruzzola F., Bourgeois D., Fueloep V., Hajdu J., Brunori M., Tegoni M., Cambillau C.
Structure 5:1157-1171(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
Strain: NTCC 6750.
[6]"Conformational changes occurring upon reduction and NO binding in nitrite reductase from Pseudomonas aeruginosa."
Nurizzo D., Cutruzzola F., Arese M., Bourgeois D., Brunori M., Cambillau C., Tegoni M.
Biochemistry 37:13987-13996(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).
Strain: NTCC 6750.
[7]"Does the reduction of c heme trigger the conformational change of crystalline nitrite reductase?"
Nurizzo D., Cutruzzola F., Arese M., Bourgeois D., Brunori M., Cambillau C., Tegoni M.
J. Biol. Chem. 274:14997-15004(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
Strain: NTCC 6750.
[8]"The nitrite reductase from Pseudomonas aeruginosa: essential role of two active-site histidines in the catalytic and structural properties."
Cutruzzola F., Brown K., Wilson E.K., Bellelli A., Arese M., Tegoni M., Cambillau C., Brunori B.
Proc. Natl. Acad. Sci. U.S.A. 98:2232-2237(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF H394A AND H352A MUTANTS.
[9]"Domain swing upon His to Ala mutation in nitrite reductase of Pseudomonas aeruginosa."
Brown K., Roig-Zamboni V., Cutruzzola F., Arese M., Sun W., Brunori M., Cambillau C., Tegoni M.
J. Mol. Biol. 312:541-554(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF H394A AND H352A MUTANTS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X16452 Genomic DNA. Translation: CAA34471.1.
AE004091 Genomic DNA. Translation: AAG03908.1.
X51631 Genomic DNA. Translation: CAA35957.1.
X51319 Genomic DNA. Translation: CAA35702.1.
PIROSPSA. A32975.
RefSeqNP_249210.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BL9X-ray2.90A/B26-568[»]
1GJQX-ray2.70A/B26-568[»]
1HZUX-ray2.70A26-568[»]
1HZVX-ray2.83A26-568[»]
1N15X-ray2.90A/B26-568[»]
1N50X-ray2.90A/B26-568[»]
1N90X-ray2.90A/B26-568[»]
1NIRX-ray2.15A/B26-568[»]
1NNOX-ray2.65A/B26-568[»]
ProteinModelPortalP24474.
SMRP24474. Positions 30-568.
ModBaseSearch...

Protein-protein interaction databases

STRING208964.PA0519.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID882217.
KEGGpae:PA0519.
PATRIC19835296. VBIPseAer58763_0549.

Organism-specific databases

PseudoCAPPA0519.

Phylogenomic databases

eggNOGCOG2010.
HOGENOMHOG000225986.
KOK15864.
OMAAGMPNWG.
ProtClustDBCLSK865915.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProIPR009056. Cyt_c_dom.
IPR011048. Cyt_cd1_nitrite_Rdtase_haem_C.
IPR003143. Cyt_d1_haem_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
SUPFAMSSF51004. Cyt_cd1_haem_C. 1 hit.
SSF46626. Cytochrome_c. 1 hit.
PROSITEPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP24474.

Entry information

Entry nameNIRS_PSEAE
AccessionPrimary (citable) accession number: P24474
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents