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Protein

Glutathione S-transferase kappa 1

Gene

Gstk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Might confer protection against genotoxic and cytotoxic electrophiles in the mitochondrial compartment.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53Glutathione1 Publication1
Binding sitei183Glutathione; via amide nitrogen and carbonyl oxygen1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-RNO-156590. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase kappa 1 (EC:2.5.1.18)
Alternative name(s):
GST 13-13
GST class-kappa
GSTK1-1
Short name:
rGSTK1
Glutathione S-transferase subunit 13
Gene namesi
Name:Gstk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi735188. Gstk1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial matrix Source: HGNC
  • peroxisome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16S → A: Reduces catalytic activity about 30-fold. Reduces affinity for glutathione about 4-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001858932 – 226Glutathione S-transferase kappa 1Add BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36N6-succinyllysineBy similarity1
Modified residuei49N6-succinyllysineBy similarity1
Modified residuei68N6-acetyllysine; alternateBy similarity1
Modified residuei68N6-succinyllysine; alternateBy similarity1
Modified residuei74N6-acetyllysine; alternateBy similarity1
Modified residuei74N6-succinyllysine; alternateBy similarity1
Modified residuei85N6-acetyllysineBy similarity1
Modified residuei93N6-acetyllysine; alternateBy similarity1
Modified residuei93N6-succinyllysine; alternateBy similarity1
Modified residuei116N6-acetyllysine; alternateBy similarity1
Modified residuei116N6-succinyllysine; alternateBy similarity1
Modified residuei144N6-succinyllysineBy similarity1
Modified residuei158N6-acetyllysine; alternateBy similarity1
Modified residuei158N6-succinyllysine; alternateBy similarity1
Modified residuei165N6-acetyllysineBy similarity1
Modified residuei167N6-acetyllysine; alternateBy similarity1
Modified residuei167N6-succinyllysine; alternateBy similarity1
Modified residuei177N6-acetyllysine; alternateBy similarity1
Modified residuei177N6-succinyllysine; alternateBy similarity1
Modified residuei193N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP24473.
PRIDEiP24473.

PTM databases

iPTMnetiP24473.
PhosphoSitePlusiP24473.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016484.
ExpressionAtlasiP24473. baseline and differential.
GenevisibleiP24473. RN.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP24473. 1 interactor.
MINTiMINT-4589693.
STRINGi10116.ENSRNOP00000022275.

Structurei

Secondary structure

1226
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Helixi17 – 29Combined sources13
Turni30 – 32Combined sources3
Beta strandi33 – 41Combined sources9
Helixi44 – 50Combined sources7
Helixi60 – 77Combined sources18
Turni87 – 89Combined sources3
Helixi90 – 94Combined sources5
Helixi97 – 109Combined sources13
Helixi111 – 113Combined sources3
Helixi114 – 126Combined sources13
Helixi135 – 144Combined sources10
Helixi149 – 156Combined sources8
Turni157 – 160Combined sources4
Helixi162 – 177Combined sources16
Beta strandi185 – 190Combined sources6
Beta strandi193 – 199Combined sources7
Helixi203 – 210Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4WX-ray2.50A/B/C/D1-226[»]
ProteinModelPortaliP24473.
SMRiP24473.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24473.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 18Glutathione binding3
Regioni200 – 201Glutathione binding2

Sequence similaritiesi

Belongs to the GST superfamily. Kappa family.Curated

Phylogenomic databases

eggNOGiENOG410IJK9. Eukaryota.
COG3917. LUCA.
GeneTreeiENSGT00440000033697.
HOGENOMiHOG000219769.
HOVERGENiHBG051852.
InParanoidiP24473.
KOiK13299.
OMAiELWRRIW.
OrthoDBiEOG091G0LCM.
PhylomeDBiP24473.
TreeFamiTF105323.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR001853. DSBA-like_thioredoxin_dom.
IPR014440. HCCAis_GSTk.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF01323. DSBA. 1 hit.
[Graphical view]
PIRSFiPIRSF006386. HCCAis_GSTk. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPAPRVLEL FYDVLSPYSW LGFEVLCRYQ HLWNIKLKLR PALLAGIMKD
60 70 80 90 100
SGNQPPAMVP HKGQYILKEI PLLKQLFQVP MSVPKDFFGE HVKKGTVNAM
110 120 130 140 150
RFLTAVSMEQ PEMLEKVSRE LWMRIWSRDE DITESQNILS AAEKAGMATA
160 170 180 190 200
QAQHLLNKIS TELVKSKLRE TTGAACKYGA FGLPTTVAHV DGKTYMLFGS
210 220
DRMELLAYLL GEKWMGPVPP TLNARL
Length:226
Mass (Da):25,493
Last modified:January 23, 2007 - v3
Checksum:iD1E5514C5A65B3CC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2G → C AA sequence (PubMed:1883325).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83436 mRNA. Translation: AAB50831.1.
PIRiS17164.
RefSeqiNP_852036.1. NM_181371.2.
UniGeneiRn.109452.

Genome annotation databases

EnsembliENSRNOT00000022275; ENSRNOP00000022275; ENSRNOG00000016484.
GeneIDi297029.
KEGGirno:297029.
UCSCiRGD:735188. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83436 mRNA. Translation: AAB50831.1.
PIRiS17164.
RefSeqiNP_852036.1. NM_181371.2.
UniGeneiRn.109452.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4WX-ray2.50A/B/C/D1-226[»]
ProteinModelPortaliP24473.
SMRiP24473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24473. 1 interactor.
MINTiMINT-4589693.
STRINGi10116.ENSRNOP00000022275.

PTM databases

iPTMnetiP24473.
PhosphoSitePlusiP24473.

Proteomic databases

PaxDbiP24473.
PRIDEiP24473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022275; ENSRNOP00000022275; ENSRNOG00000016484.
GeneIDi297029.
KEGGirno:297029.
UCSCiRGD:735188. rat.

Organism-specific databases

CTDi373156.
RGDi735188. Gstk1.

Phylogenomic databases

eggNOGiENOG410IJK9. Eukaryota.
COG3917. LUCA.
GeneTreeiENSGT00440000033697.
HOGENOMiHOG000219769.
HOVERGENiHBG051852.
InParanoidiP24473.
KOiK13299.
OMAiELWRRIW.
OrthoDBiEOG091G0LCM.
PhylomeDBiP24473.
TreeFamiTF105323.

Enzyme and pathway databases

ReactomeiR-RNO-156590. Glutathione conjugation.

Miscellaneous databases

EvolutionaryTraceiP24473.
PROiP24473.

Gene expression databases

BgeeiENSRNOG00000016484.
ExpressionAtlasiP24473. baseline and differential.
GenevisibleiP24473. RN.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR001853. DSBA-like_thioredoxin_dom.
IPR014440. HCCAis_GSTk.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF01323. DSBA. 1 hit.
[Graphical view]
PIRSFiPIRSF006386. HCCAis_GSTk. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSTK1_RAT
AccessioniPrimary (citable) accession number: P24473
Secondary accession number(s): O09034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.