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P24473

- GSTK1_RAT

UniProt

P24473 - GSTK1_RAT

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Protein
Glutathione S-transferase kappa 1
Gene
Gstk1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Might confer protection against genotoxic and cytotoxic electrophiles in the mitochondrial compartment.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531Glutathione
Binding sitei183 – 1831Glutathione; via amide nitrogen and carbonyl oxygen

GO - Molecular functioni

  1. glutathione peroxidase activity Source: Ensembl
  2. glutathione transferase activity Source: UniProtKB-EC
  3. protein disulfide oxidoreductase activity Source: InterPro

GO - Biological processi

  1. glutathione metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase kappa 1 (EC:2.5.1.18)
Alternative name(s):
GST 13-13
GST class-kappa
GSTK1-1
Short name:
rGSTK1
Glutathione S-transferase subunit 13
Gene namesi
Name:Gstk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi735188. Gstk1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: Ensembl
  2. mitochondrial matrix Source: HGNC
  3. peroxisome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi16 – 161S → A: Reduces catalytic activity about 30-fold. Reduces affinity for glutathione about 4-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 226225Glutathione S-transferase kappa 1
PRO_0000185893Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361N6-succinyllysine By similarity
Modified residuei49 – 491N6-succinyllysine By similarity
Modified residuei68 – 681N6-acetyllysine; alternate By similarity
Modified residuei68 – 681N6-succinyllysine; alternate By similarity
Modified residuei74 – 741N6-acetyllysine; alternate By similarity
Modified residuei74 – 741N6-succinyllysine; alternate By similarity
Modified residuei85 – 851N6-acetyllysine By similarity
Modified residuei93 – 931N6-acetyllysine; alternate By similarity
Modified residuei93 – 931N6-succinyllysine; alternate By similarity
Modified residuei116 – 1161N6-acetyllysine; alternate By similarity
Modified residuei116 – 1161N6-succinyllysine; alternate By similarity
Modified residuei144 – 1441N6-succinyllysine By similarity
Modified residuei158 – 1581N6-acetyllysine; alternate By similarity
Modified residuei158 – 1581N6-succinyllysine; alternate By similarity
Modified residuei165 – 1651N6-acetyllysine By similarity
Modified residuei167 – 1671N6-acetyllysine; alternate By similarity
Modified residuei167 – 1671N6-succinyllysine; alternate By similarity
Modified residuei177 – 1771N6-acetyllysine; alternate By similarity
Modified residuei177 – 1771N6-succinyllysine; alternate By similarity
Modified residuei193 – 1931N6-succinyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP24473.
PRIDEiP24473.

PTM databases

PhosphoSiteiP24473.

Expressioni

Gene expression databases

GenevestigatoriP24473.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP24473. 1 interaction.
MINTiMINT-4589693.
STRINGi10116.ENSRNOP00000022275.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127
Helixi17 – 2913
Turni30 – 323
Beta strandi33 – 419
Helixi44 – 507
Helixi60 – 7718
Turni87 – 893
Helixi90 – 945
Helixi97 – 10913
Helixi111 – 1133
Helixi114 – 12613
Helixi135 – 14410
Helixi149 – 1568
Turni157 – 1604
Helixi162 – 17716
Beta strandi185 – 1906
Beta strandi193 – 1997
Helixi203 – 2108

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4WX-ray2.50A/B/C/D1-226[»]
ProteinModelPortaliP24473.
SMRiP24473. Positions 2-222.

Miscellaneous databases

EvolutionaryTraceiP24473.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 183Glutathione binding
Regioni200 – 2012Glutathione binding

Sequence similaritiesi

Belongs to the GST superfamily. Kappa family.

Phylogenomic databases

eggNOGiCOG3917.
GeneTreeiENSGT00440000033697.
HOGENOMiHOG000219769.
HOVERGENiHBG051852.
InParanoidiP24473.
KOiK13299.
OMAiELWRRIW.
OrthoDBiEOG793B9B.
PhylomeDBiP24473.
TreeFamiTF105323.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR001853. DSBA-like_thioredoxin_dom.
IPR014440. HCCAis_GSTk.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF01323. DSBA. 1 hit.
[Graphical view]
PIRSFiPIRSF006386. HCCAis_GSTk. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24473-1 [UniParc]FASTAAdd to Basket

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MGPAPRVLEL FYDVLSPYSW LGFEVLCRYQ HLWNIKLKLR PALLAGIMKD    50
SGNQPPAMVP HKGQYILKEI PLLKQLFQVP MSVPKDFFGE HVKKGTVNAM 100
RFLTAVSMEQ PEMLEKVSRE LWMRIWSRDE DITESQNILS AAEKAGMATA 150
QAQHLLNKIS TELVKSKLRE TTGAACKYGA FGLPTTVAHV DGKTYMLFGS 200
DRMELLAYLL GEKWMGPVPP TLNARL 226
Length:226
Mass (Da):25,493
Last modified:January 23, 2007 - v3
Checksum:iD1E5514C5A65B3CC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21G → C AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S83436 mRNA. Translation: AAB50831.1.
PIRiS17164.
RefSeqiNP_852036.1. NM_181371.2.
UniGeneiRn.109452.

Genome annotation databases

EnsembliENSRNOT00000022275; ENSRNOP00000022275; ENSRNOG00000016484.
GeneIDi297029.
KEGGirno:297029.
UCSCiRGD:735188. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S83436 mRNA. Translation: AAB50831.1 .
PIRi S17164.
RefSeqi NP_852036.1. NM_181371.2.
UniGenei Rn.109452.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1R4W X-ray 2.50 A/B/C/D 1-226 [» ]
ProteinModelPortali P24473.
SMRi P24473. Positions 2-222.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P24473. 1 interaction.
MINTi MINT-4589693.
STRINGi 10116.ENSRNOP00000022275.

Chemistry

DrugBanki DB00143. Glutathione.

PTM databases

PhosphoSitei P24473.

Proteomic databases

PaxDbi P24473.
PRIDEi P24473.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000022275 ; ENSRNOP00000022275 ; ENSRNOG00000016484 .
GeneIDi 297029.
KEGGi rno:297029.
UCSCi RGD:735188. rat.

Organism-specific databases

CTDi 373156.
RGDi 735188. Gstk1.

Phylogenomic databases

eggNOGi COG3917.
GeneTreei ENSGT00440000033697.
HOGENOMi HOG000219769.
HOVERGENi HBG051852.
InParanoidi P24473.
KOi K13299.
OMAi ELWRRIW.
OrthoDBi EOG793B9B.
PhylomeDBi P24473.
TreeFami TF105323.

Miscellaneous databases

EvolutionaryTracei P24473.
NextBioi 642014.
PROi P24473.

Gene expression databases

Genevestigatori P24473.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR001853. DSBA-like_thioredoxin_dom.
IPR014440. HCCAis_GSTk.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF01323. DSBA. 1 hit.
[Graphical view ]
PIRSFi PIRSF006386. HCCAis_GSTk. 1 hit.
SUPFAMi SSF52833. SSF52833. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Glutathione S-transferase class Kappa: characterization by the cloning of rat mitochondrial GST and identification of a human homologue."
    Pemble S.E., Wardle A.F., Taylor J.B.
    Biochem. J. 319:749-754(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "A novel glutathione transferase (13-13) isolated from the matrix of rat liver mitochondria having structural similarity to class theta enzymes."
    Harris M.J., Meyer D.J., Coles B., Ketterer B.
    Biochem. J. 278:137-141(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-34.
  3. "Parallel evolutionary pathways for glutathione transferases: structure and mechanism of the mitochondrial class kappa enzyme rGSTK1-1."
    Ladner J.E., Parsons J.F., Rife C.L., Gilliland G.L., Armstrong R.N.
    Biochemistry 43:352-361(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE, SUBUNIT, MUTAGENESIS OF SER-16.

Entry informationi

Entry nameiGSTK1_RAT
AccessioniPrimary (citable) accession number: P24473
Secondary accession number(s): O09034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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