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Protein

Glutathione S-transferase A4

Gene

Gsta4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.5.1.18. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A4 (EC:2.5.1.18)
Alternative name(s):
GST A4-4
Short name:
GSTA4-4
GST class-alpha member 4
Glutathione S-transferase 5.7
Short name:
GST 5.7
Gene namesi
Name:Gsta4
Synonyms:Gsta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1309515. Gsta4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857911 – 222Glutathione S-transferase A4Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP24472.
PaxDbiP24472.
PeptideAtlasiP24472.
PRIDEiP24472.
TopDownProteomicsiP24472.

2D gel databases

REPRODUCTION-2DPAGEP24472.

PTM databases

iPTMnetiP24472.
PhosphoSitePlusiP24472.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032348.
CleanExiMM_GSTA4.
GenevisibleiP24472. MM.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi200093. 1 interactor.
IntActiP24472. 2 interactors.
MINTiMINT-1487605.
STRINGi10090.ENSMUSP00000034903.

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Turni14 – 16Combined sources3
Helixi17 – 26Combined sources10
Beta strandi31 – 34Combined sources4
Helixi38 – 45Combined sources8
Turni46 – 48Combined sources3
Beta strandi50 – 53Combined sources4
Beta strandi57 – 60Combined sources4
Beta strandi63 – 65Combined sources3
Helixi68 – 78Combined sources11
Helixi86 – 108Combined sources23
Helixi109 – 111Combined sources3
Helixi114 – 131Combined sources18
Helixi133 – 142Combined sources10
Beta strandi145 – 152Combined sources8
Helixi155 – 168Combined sources14
Helixi174 – 177Combined sources4
Helixi179 – 189Combined sources11
Helixi192 – 198Combined sources7
Beta strandi199 – 203Combined sources5
Helixi210 – 219Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B48X-ray2.60A/B2-222[»]
1GUKX-ray2.90A/B1-222[»]
ProteinModelPortaliP24472.
SMRiP24472.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24472.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 208GST C-terminalAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 55Glutathione bindingCombined sources1 Publication3
Regioni66 – 68Glutathione bindingCombined sources1 Publication3

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP24472.
KOiK00799.
OMAiMGMIMYL.
OrthoDBiEOG091G0O3D.
PhylomeDBiP24472.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24472-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKPKLYYF NGRGRMESIR WLLAAAGVEF EEEFLETREQ YEKMQKDGHL
60 70 80 90 100
LFGQVPLVEI DGMMLTQTRA ILSYLAAKYN LYGKDLKERV RIDMYADGTQ
110 120 130 140 150
DLMMMIAVAP FKTPKEKEES YDLILSRAKT RYFPVFEKIL KDHGEAFLVG
160 170 180 190 200
NQLSWADIQL LEAILMVEEL SAPVLSDFPL LQAFKTRISN IPTIKKFLQP
210 220
GSQRKPPPDG PYVEVVRTVL KF
Length:222
Mass (Da):25,564
Last modified:January 23, 2002 - v3
Checksum:iB580E3415289424D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36E → D in BAB31640 (PubMed:16141072).Curated1
Sequence conflicti205 – 206KP → SA in BAB27873 (PubMed:16141072).Curated2
Sequence conflicti218T → I in AAA37754 (PubMed:1426286).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti115K → P Requires 2 nucleotide substitutions. 1
Natural varianti167V → G.1
Natural varianti179 – 180PL → GE.2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06047 mRNA. Translation: AAA37754.1.
AK008189 mRNA. Translation: BAB25520.1.
AK008193 mRNA. Translation: BAB25524.1.
AK008400 mRNA. Translation: BAB25649.1.
AK008490 mRNA. Translation: BAB25696.1.
AK009668 mRNA. Translation: BAB26429.1.
AK010098 mRNA. Translation: BAB26701.1.
AK011177 mRNA. Translation: BAB27449.1.
AK011841 mRNA. Translation: BAB27873.1.
AK019100 mRNA. Translation: BAB31546.1.
AK019271 mRNA. Translation: BAB31640.1.
BC012639 mRNA. Translation: AAH12639.1.
CCDSiCCDS23357.1.
PIRiS27234.
RefSeqiNP_034487.2. NM_010357.3.
UniGeneiMm.2662.

Genome annotation databases

EnsembliENSMUST00000034903; ENSMUSP00000034903; ENSMUSG00000032348.
GeneIDi14860.
KEGGimmu:14860.
UCSCiuc009qty.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06047 mRNA. Translation: AAA37754.1.
AK008189 mRNA. Translation: BAB25520.1.
AK008193 mRNA. Translation: BAB25524.1.
AK008400 mRNA. Translation: BAB25649.1.
AK008490 mRNA. Translation: BAB25696.1.
AK009668 mRNA. Translation: BAB26429.1.
AK010098 mRNA. Translation: BAB26701.1.
AK011177 mRNA. Translation: BAB27449.1.
AK011841 mRNA. Translation: BAB27873.1.
AK019100 mRNA. Translation: BAB31546.1.
AK019271 mRNA. Translation: BAB31640.1.
BC012639 mRNA. Translation: AAH12639.1.
CCDSiCCDS23357.1.
PIRiS27234.
RefSeqiNP_034487.2. NM_010357.3.
UniGeneiMm.2662.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B48X-ray2.60A/B2-222[»]
1GUKX-ray2.90A/B1-222[»]
ProteinModelPortaliP24472.
SMRiP24472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200093. 1 interactor.
IntActiP24472. 2 interactors.
MINTiMINT-1487605.
STRINGi10090.ENSMUSP00000034903.

PTM databases

iPTMnetiP24472.
PhosphoSitePlusiP24472.

2D gel databases

REPRODUCTION-2DPAGEP24472.

Proteomic databases

MaxQBiP24472.
PaxDbiP24472.
PeptideAtlasiP24472.
PRIDEiP24472.
TopDownProteomicsiP24472.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034903; ENSMUSP00000034903; ENSMUSG00000032348.
GeneIDi14860.
KEGGimmu:14860.
UCSCiuc009qty.2. mouse.

Organism-specific databases

CTDi2941.
MGIiMGI:1309515. Gsta4.

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP24472.
KOiK00799.
OMAiMGMIMYL.
OrthoDBiEOG091G0O3D.
PhylomeDBiP24472.
TreeFamiTF105321.

Enzyme and pathway databases

BRENDAi2.5.1.18. 3474.

Miscellaneous databases

EvolutionaryTraceiP24472.
PROiP24472.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032348.
CleanExiMM_GSTA4.
GenevisibleiP24472. MM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA4_MOUSE
AccessioniPrimary (citable) accession number: P24472
Secondary accession number(s): Q9CQ81
, Q9CTY7, Q9CY87, Q9D038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2002
Last modified: November 2, 2016
This is version 163 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

On the basis of immunological and kinetics data, GST 5.7 is distinct from alpha, mu and pI classes of GTS. However it has been postulated that this protein may be part of a distinct subgroup within this alpha class.
The variations were found from AA sequencing and imply there are multiple forms of this protein. These variations are likely to be sex-linked and tissue specific.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.