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P24470

- CP2CN_RAT

UniProt

P24470 - CP2CN_RAT

Protein

Cytochrome P450 2C23

Gene

Cyp2c23

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Predominant enzyme isoform responsible for arachidonic acid epoxidation in the rat kidney. Catalyzes the NADPH-dependent metabolism of arachidonic acid to a mixture of 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (EETS) as the only oxygenation products. Low rate of lauric acid hydroxylation at the (omega-1)-position.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. arachidonic acid epoxygenase activity Source: RGD
    2. aromatase activity Source: UniProtKB-EC
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro

    GO - Biological processi

    1. arachidonic acid metabolic process Source: RGD
    2. response to testosterone Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2C23 (EC:1.14.14.1)
    Alternative name(s):
    Arachidonic acid epoxygenase
    CYPIIC23
    Gene namesi
    Name:Cyp2c23
    Synonyms:Cyp2c-23
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620370. Cyp2c23.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Cytochrome P450 2C23PRO_0000051711Add
    BLAST

    Proteomic databases

    PaxDbiP24470.
    PRIDEiP24470.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney and liver; undetectable in brain and heart.

    Inductioni

    Constitutively expressed.

    Gene expression databases

    GenevestigatoriP24470.

    Interactioni

    Protein-protein interaction databases

    IntActiP24470. 1 interaction.
    MINTiMINT-4567268.

    Structurei

    3D structure databases

    ProteinModelPortaliP24470.
    SMRiP24470. Positions 31-493.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiP24470.
    PhylomeDBiP24470.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24470-1 [UniParc]FASTAAdd to Basket

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    MELLGFTTLA LVVSVTCLSL LSVWTKLRTR GRLPPGPTPL PIIGNLLQLN    50
    LKDIPASLSK LAKEYGPVYT LYFGTSPTVV LHGYDVVKEA LLQQGDEFLG 100
    RGPLPIIEDT HKGYGLIFSN GERWKVMRRF SLMTLRNFGM GKRSLEERVQ 150
    EEARCLVEEL QKTKAQPFDP TFILACAPCN VICSILFNDR FQYNDKTFLN 200
    LMDLLNKNFQ QVNSVWCQMY NLWPTIIKYL PGKHIEFAKR IDDVKNFILE 250
    KVKEHQKSLD PANPRDYIDC FLSKIEEEKD NLKSEFHLEN LAVCGSNLFT 300
    AGTETTSTTL RFGLLLLMKY PEVQAKVHEE LDRVIGRHQP PSMKDKMKLP 350
    YTDAVLHEIQ RYITLLPSSL PHAVVQDTKF RDYVIPKGTT VLPMLSSVML 400
    DQKEFANPEK FDPGHFLDKN GCFKKTDYFV PFSLGKRACV GESLARMELF 450
    LFFTTLLQKF SLKTLVEPKD LDIKPITTGI INLPPPYKLC LVPR 494
    Length:494
    Mass (Da):56,433
    Last modified:July 15, 1999 - v2
    Checksum:iBB6529F9953B540E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 5417TPLPI…NLKDI → HPPSHYWESTATEPQGH in CAA39087. (PubMed:2263487)CuratedAdd
    BLAST
    Sequence conflicti154 – 1541R → W in CAA39087. (PubMed:2263487)Curated
    Sequence conflicti366 – 3672LP → VG in CAA39087. (PubMed:2263487)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55446 mRNA. Translation: CAA39087.1.
    U04733 mRNA. Translation: AAA03716.1.
    S67064 mRNA. Translation: AAB29022.1.
    PIRiA46588.
    S13101.
    UniGeneiRn.2184.

    Genome annotation databases

    UCSCiRGD:620370. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55446 mRNA. Translation: CAA39087.1 .
    U04733 mRNA. Translation: AAA03716.1 .
    S67064 mRNA. Translation: AAB29022.1 .
    PIRi A46588.
    S13101.
    UniGenei Rn.2184.

    3D structure databases

    ProteinModelPortali P24470.
    SMRi P24470. Positions 31-493.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P24470. 1 interaction.
    MINTi MINT-4567268.

    Chemistry

    BindingDBi P24470.
    ChEMBLi CHEMBL2772.

    Proteomic databases

    PaxDbi P24470.
    PRIDEi P24470.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:620370. rat.

    Organism-specific databases

    RGDi 620370. Cyp2c23.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi P24470.
    PhylomeDBi P24470.

    Gene expression databases

    Genevestigatori P24470.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA and deduced amino acid sequence of a novel cytochrome P-450 from female rat liver mRNA with high homology to P-450 IIC family."
      Cook E.A., Groenewegen W.A., Gloger I.S., Piggott J.R., Suckling K.E., Wolf C.R.
      Nucleic Acids Res. 18:7156-7156(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Liver.
    2. "Molecular cloning, expression, and enzymatic characterization of the rat kidney cytochrome P-450 arachidonic acid epoxygenase."
      Karara A., Makita K., Jacobson H.R., Falk J.R., Guengerich P.F., Dubois R.N., Capdevila J.H.
      J. Biol. Chem. 268:13565-13570(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    3. "Identification of CYP2C23 expressed in rat kidney as an arachidonic acid epoxygenase."
      Imaoka S., Wedlund P.J., Ogawa H., Kimura S., Gonzalez F.J., Kim H.Y.
      J. Pharmacol. Exp. Ther. 267:1012-1016(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-86.
      Tissue: Kidney.

    Entry informationi

    Entry nameiCP2CN_RAT
    AccessioniPrimary (citable) accession number: P24470
    Secondary accession number(s): Q63914, Q64534
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3