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Protein

Cytochrome P450 2C23

Gene

Cyp2c23

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Predominant enzyme isoform responsible for arachidonic acid epoxidation in the rat kidney. Catalyzes the NADPH-dependent metabolism of arachidonic acid to a mixture of 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (EETS) as the only oxygenation products. Low rate of lauric acid hydroxylation at the (omega-1)-position.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: RGD
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  6. oxygen binding Source: GO_Central
  7. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. arachidonic acid metabolic process Source: RGD
  2. epoxygenase P450 pathway Source: GO_Central
  3. exogenous drug catabolic process Source: GO_Central
  4. oxidation-reduction process Source: GO_Central
  5. response to testosterone Source: RGD
  6. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C23 (EC:1.14.14.1)
Alternative name(s):
Arachidonic acid epoxygenase
CYPIIC23
Gene namesi
Name:Cyp2c23
Synonyms:Cyp2c-23
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620370. Cyp2c23.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 494493Cytochrome P450 2C23PRO_0000051711Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei253 – 2531N6-acetyllysineBy similarity
Modified residuei379 – 3791N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP24470.
PRIDEiP24470.

Expressioni

Tissue specificityi

Highly expressed in kidney and liver; undetectable in brain and heart.

Inductioni

Constitutively expressed.

Gene expression databases

GenevestigatoriP24470.

Interactioni

Protein-protein interaction databases

IntActiP24470. 1 interaction.
MINTiMINT-4567268.

Structurei

3D structure databases

ProteinModelPortaliP24470.
SMRiP24470. Positions 31-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP24470.
PhylomeDBiP24470.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLGFTTLA LVVSVTCLSL LSVWTKLRTR GRLPPGPTPL PIIGNLLQLN
60 70 80 90 100
LKDIPASLSK LAKEYGPVYT LYFGTSPTVV LHGYDVVKEA LLQQGDEFLG
110 120 130 140 150
RGPLPIIEDT HKGYGLIFSN GERWKVMRRF SLMTLRNFGM GKRSLEERVQ
160 170 180 190 200
EEARCLVEEL QKTKAQPFDP TFILACAPCN VICSILFNDR FQYNDKTFLN
210 220 230 240 250
LMDLLNKNFQ QVNSVWCQMY NLWPTIIKYL PGKHIEFAKR IDDVKNFILE
260 270 280 290 300
KVKEHQKSLD PANPRDYIDC FLSKIEEEKD NLKSEFHLEN LAVCGSNLFT
310 320 330 340 350
AGTETTSTTL RFGLLLLMKY PEVQAKVHEE LDRVIGRHQP PSMKDKMKLP
360 370 380 390 400
YTDAVLHEIQ RYITLLPSSL PHAVVQDTKF RDYVIPKGTT VLPMLSSVML
410 420 430 440 450
DQKEFANPEK FDPGHFLDKN GCFKKTDYFV PFSLGKRACV GESLARMELF
460 470 480 490
LFFTTLLQKF SLKTLVEPKD LDIKPITTGI INLPPPYKLC LVPR
Length:494
Mass (Da):56,433
Last modified:July 15, 1999 - v2
Checksum:iBB6529F9953B540E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 5417TPLPI…NLKDI → HPPSHYWESTATEPQGH in CAA39087 (PubMed:2263487).CuratedAdd
BLAST
Sequence conflicti154 – 1541R → W in CAA39087 (PubMed:2263487).Curated
Sequence conflicti366 – 3672LP → VG in CAA39087 (PubMed:2263487).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55446 mRNA. Translation: CAA39087.1.
U04733 mRNA. Translation: AAA03716.1.
S67064 mRNA. Translation: AAB29022.1.
PIRiA46588.
S13101.
UniGeneiRn.2184.

Genome annotation databases

UCSCiRGD:620370. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55446 mRNA. Translation: CAA39087.1.
U04733 mRNA. Translation: AAA03716.1.
S67064 mRNA. Translation: AAB29022.1.
PIRiA46588.
S13101.
UniGeneiRn.2184.

3D structure databases

ProteinModelPortaliP24470.
SMRiP24470. Positions 31-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24470. 1 interaction.
MINTiMINT-4567268.

Chemistry

BindingDBiP24470.
ChEMBLiCHEMBL2772.

Proteomic databases

PaxDbiP24470.
PRIDEiP24470.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620370. rat.

Organism-specific databases

RGDi620370. Cyp2c23.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP24470.
PhylomeDBiP24470.

Miscellaneous databases

PROiP24470.

Gene expression databases

GenevestigatoriP24470.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA and deduced amino acid sequence of a novel cytochrome P-450 from female rat liver mRNA with high homology to P-450 IIC family."
    Cook E.A., Groenewegen W.A., Gloger I.S., Piggott J.R., Suckling K.E., Wolf C.R.
    Nucleic Acids Res. 18:7156-7156(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  2. "Molecular cloning, expression, and enzymatic characterization of the rat kidney cytochrome P-450 arachidonic acid epoxygenase."
    Karara A., Makita K., Jacobson H.R., Falk J.R., Guengerich P.F., Dubois R.N., Capdevila J.H.
    J. Biol. Chem. 268:13565-13570(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. "Identification of CYP2C23 expressed in rat kidney as an arachidonic acid epoxygenase."
    Imaoka S., Wedlund P.J., Ogawa H., Kimura S., Gonzalez F.J., Kim H.Y.
    J. Pharmacol. Exp. Ther. 267:1012-1016(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-86.
    Tissue: Kidney.

Entry informationi

Entry nameiCP2CN_RAT
AccessioniPrimary (citable) accession number: P24470
Secondary accession number(s): Q63914, Q64534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 15, 1999
Last modified: April 29, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.