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P24470

- CP2CN_RAT

UniProt

P24470 - CP2CN_RAT

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Protein

Cytochrome P450 2C23

Gene
Cyp2c23, Cyp2c-23
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Predominant enzyme isoform responsible for arachidonic acid epoxidation in the rat kidney. Catalyzes the NADPH-dependent metabolism of arachidonic acid to a mixture of 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (EETS) as the only oxygenation products. Low rate of lauric acid hydroxylation at the (omega-1)-position.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: RGD
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro

GO - Biological processi

  1. arachidonic acid metabolic process Source: RGD
  2. response to testosterone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C23 (EC:1.14.14.1)
Alternative name(s):
Arachidonic acid epoxygenase
CYPIIC23
Gene namesi
Name:Cyp2c23
Synonyms:Cyp2c-23
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620370. Cyp2c23.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Cytochrome P450 2C23PRO_0000051711Add
BLAST

Proteomic databases

PaxDbiP24470.
PRIDEiP24470.

Expressioni

Tissue specificityi

Highly expressed in kidney and liver; undetectable in brain and heart.

Inductioni

Constitutively expressed.

Gene expression databases

GenevestigatoriP24470.

Interactioni

Protein-protein interaction databases

IntActiP24470. 1 interaction.
MINTiMINT-4567268.

Structurei

3D structure databases

ProteinModelPortaliP24470.
SMRiP24470. Positions 31-493.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP24470.
PhylomeDBiP24470.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24470-1 [UniParc]FASTAAdd to Basket

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MELLGFTTLA LVVSVTCLSL LSVWTKLRTR GRLPPGPTPL PIIGNLLQLN    50
LKDIPASLSK LAKEYGPVYT LYFGTSPTVV LHGYDVVKEA LLQQGDEFLG 100
RGPLPIIEDT HKGYGLIFSN GERWKVMRRF SLMTLRNFGM GKRSLEERVQ 150
EEARCLVEEL QKTKAQPFDP TFILACAPCN VICSILFNDR FQYNDKTFLN 200
LMDLLNKNFQ QVNSVWCQMY NLWPTIIKYL PGKHIEFAKR IDDVKNFILE 250
KVKEHQKSLD PANPRDYIDC FLSKIEEEKD NLKSEFHLEN LAVCGSNLFT 300
AGTETTSTTL RFGLLLLMKY PEVQAKVHEE LDRVIGRHQP PSMKDKMKLP 350
YTDAVLHEIQ RYITLLPSSL PHAVVQDTKF RDYVIPKGTT VLPMLSSVML 400
DQKEFANPEK FDPGHFLDKN GCFKKTDYFV PFSLGKRACV GESLARMELF 450
LFFTTLLQKF SLKTLVEPKD LDIKPITTGI INLPPPYKLC LVPR 494
Length:494
Mass (Da):56,433
Last modified:July 15, 1999 - v2
Checksum:iBB6529F9953B540E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 5417TPLPI…NLKDI → HPPSHYWESTATEPQGH in CAA39087. 1 PublicationAdd
BLAST
Sequence conflicti154 – 1541R → W in CAA39087. 1 Publication
Sequence conflicti366 – 3672LP → VG in CAA39087. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55446 mRNA. Translation: CAA39087.1.
U04733 mRNA. Translation: AAA03716.1.
S67064 mRNA. Translation: AAB29022.1.
PIRiA46588.
S13101.
UniGeneiRn.2184.

Genome annotation databases

UCSCiRGD:620370. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55446 mRNA. Translation: CAA39087.1 .
U04733 mRNA. Translation: AAA03716.1 .
S67064 mRNA. Translation: AAB29022.1 .
PIRi A46588.
S13101.
UniGenei Rn.2184.

3D structure databases

ProteinModelPortali P24470.
SMRi P24470. Positions 31-493.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P24470. 1 interaction.
MINTi MINT-4567268.

Chemistry

BindingDBi P24470.
ChEMBLi CHEMBL2772.

Proteomic databases

PaxDbi P24470.
PRIDEi P24470.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620370. rat.

Organism-specific databases

RGDi 620370. Cyp2c23.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi P24470.
PhylomeDBi P24470.

Gene expression databases

Genevestigatori P24470.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA and deduced amino acid sequence of a novel cytochrome P-450 from female rat liver mRNA with high homology to P-450 IIC family."
    Cook E.A., Groenewegen W.A., Gloger I.S., Piggott J.R., Suckling K.E., Wolf C.R.
    Nucleic Acids Res. 18:7156-7156(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  2. "Molecular cloning, expression, and enzymatic characterization of the rat kidney cytochrome P-450 arachidonic acid epoxygenase."
    Karara A., Makita K., Jacobson H.R., Falk J.R., Guengerich P.F., Dubois R.N., Capdevila J.H.
    J. Biol. Chem. 268:13565-13570(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. "Identification of CYP2C23 expressed in rat kidney as an arachidonic acid epoxygenase."
    Imaoka S., Wedlund P.J., Ogawa H., Kimura S., Gonzalez F.J., Kim H.Y.
    J. Pharmacol. Exp. Ther. 267:1012-1016(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-86.
    Tissue: Kidney.

Entry informationi

Entry nameiCP2CN_RAT
AccessioniPrimary (citable) accession number: P24470
Secondary accession number(s): Q63914, Q64534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 15, 1999
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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