Cytochrome P450 2C23 - Rattus norvegicus (Rat)

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P24470 (CP2CN_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C23
EC=1.14.14.1

Alternative name(s):
Arachidonic acid epoxygenase
CYPIIC23

Gene names

Name:	Cyp2c23
Synonyms:	Cyp2c-23

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Predominant enzyme isoform responsible for arachidonic acid epoxidation in the rat kidney. Catalyzes the NADPH-dependent metabolism of arachidonic acid to a mixture of 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (EETS) as the only oxygenation products. Low rate of lauric acid hydroxylation at the (omega-1)-position.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Highly expressed in kidney and liver; undetectable in brain and heart.
Induction	Constitutively expressed.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arachidonic acid metabolic process Non-traceable author statement Ref.2. Source: RGD response to testosterone stimulus Inferred from expression pattern PubMed 18276983. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular membrane-bounded organelle Inferred from direct assay Ref.2. Source: RGD
Molecular_function	arachidonic acid epoxygenase activity Inferred from direct assay Ref.2. Source: RGD aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 494

494

Cytochrome P450 2C23

PRO_0000051711

Sites

Metal binding

439

Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict

38 – 54

TPLPI…NLKDI → HPPSHYWESTATEPQGH in CAA39087. Ref.1

Sequence conflict

154

R → W in CAA39087. Ref.1

Sequence conflict

366 – 367

LP → VG in CAA39087. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P24470 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: BB6529F9953B540E
Show »

FASTA

494

56,433

        10         20         30         40         50         60 
MELLGFTTLA LVVSVTCLSL LSVWTKLRTR GRLPPGPTPL PIIGNLLQLN LKDIPASLSK 

        70         80         90        100        110        120 
LAKEYGPVYT LYFGTSPTVV LHGYDVVKEA LLQQGDEFLG RGPLPIIEDT HKGYGLIFSN 

       130        140        150        160        170        180 
GERWKVMRRF SLMTLRNFGM GKRSLEERVQ EEARCLVEEL QKTKAQPFDP TFILACAPCN 

       190        200        210        220        230        240 
VICSILFNDR FQYNDKTFLN LMDLLNKNFQ QVNSVWCQMY NLWPTIIKYL PGKHIEFAKR 

       250        260        270        280        290        300 
IDDVKNFILE KVKEHQKSLD PANPRDYIDC FLSKIEEEKD NLKSEFHLEN LAVCGSNLFT 

       310        320        330        340        350        360 
AGTETTSTTL RFGLLLLMKY PEVQAKVHEE LDRVIGRHQP PSMKDKMKLP YTDAVLHEIQ 

       370        380        390        400        410        420 
RYITLLPSSL PHAVVQDTKF RDYVIPKGTT VLPMLSSVML DQKEFANPEK FDPGHFLDKN 

       430        440        450        460        470        480 
GCFKKTDYFV PFSLGKRACV GESLARMELF LFFTTLLQKF SLKTLVEPKD LDIKPITTGI 

       490 
INLPPPYKLC LVPR

« Hide

References

[1]	"cDNA and deduced amino acid sequence of a novel cytochrome P-450 from female rat liver mRNA with high homology to P-450 IIC family." Cook E.A., Groenewegen W.A., Gloger I.S., Piggott J.R., Suckling K.E., Wolf C.R. Nucleic Acids Res. 18:7156-7156(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[2]	"Molecular cloning, expression, and enzymatic characterization of the rat kidney cytochrome P-450 arachidonic acid epoxygenase." Karara A., Makita K., Jacobson H.R., Falk J.R., Guengerich P.F., Dubois R.N., Capdevila J.H. J. Biol. Chem. 268:13565-13570(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney.
[3]	"Identification of CYP2C23 expressed in rat kidney as an arachidonic acid epoxygenase." Imaoka S., Wedlund P.J., Ogawa H., Kimura S., Gonzalez F.J., Kim H.Y. J. Pharmacol. Exp. Ther. 267:1012-1016(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-86. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55446 mRNA. Translation: CAA39087.1. U04733 mRNA. Translation: AAA03716.1. S67064 mRNA. Translation: AAB29022.1.
IPI	IPI00327991.
PIR	A46588. S13101.
UniGene	Rn.2184.
3D structure databases
ProteinModelPortal	P24470.
SMR	P24470. Positions 31-493.
ModBase	Search...
Protein-protein interaction databases
IntAct	P24470. 1 interaction.
Proteomic databases
PaxDb	P24470.
PRIDE	P24470.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:620370. rat.
Organism-specific databases
RGD	620370. Cyp2c23.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	P24470.
OrthoDB	EOG48WC22.
Gene expression databases
ArrayExpress	P24470.
Genevestigator	P24470.
GermOnline	ENSRNOG00000013291. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P24470.
ChEMBL	CHEMBL2772.

Entry information

Entry name

CP2CN_RAT

Accession

Primary (citable) accession number: P24470
Secondary accession number(s): Q63914, Q64534

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents