Cytochrome P450 4A12 - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P24464 (CP4AC_RAT)

Last modified January 19, 2010. Version 81. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Cytochrome P450 4A12
    EC=1.14.14.1
Alternative name(s):
    CYPIVA12
    CYPIVA8
    Cytochrome P450-KP1
    Cytochrome P450-PP1

Gene names

Name:	Cyp4a12
Synonyms:	Cyp4a-8, Cyp4a8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

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Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	arachidonic acid metabolic process Inferred from direct assay. Source: RGD kidney development Inferred from expression pattern. Source: RGD lauric acid metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to testosterone stimulus Inferred from expression pattern. Source: RGD
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Traceable author statement. Source: UniProtKB
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 508

508

Cytochrome P450 4A12

PRO_0000051818

Sites

Metal binding

455

Iron (heme axial ligand)

Binding site

319

Heme (covalent; via 1 link)

Experimental info

Sequence conflict

F → L in AAA63485. Ref.1

Sequence conflict

E → D in AAA63485. Ref.1

Sequence conflict

101

D → E in AAA63485. Ref.1

Sequence conflict

114 – 116

SHH → AHG in AAA63485. Ref.1

Sequence conflict

226

S → F in AAA63485. Ref.1

Sequence conflict

230 – 233

IRNI → VQNM in AAA63485. Ref.1

Sequence conflict

239

I → F in AAA63485. Ref.1

Sequence conflict

251 – 252

RS → HN in AAA63485. Ref.1

Sequence conflict

259 – 260

EH → DY in AAA63485. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P24464-1 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: 2C2218525FC65513
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FASTA

508

58,559

        10         20         30         40         50         60 
MSGSALSFTI FPGSILGFLQ IATVLTVLLL LFKTAQFYLH RRWLLRATQQ FPSPPSHWFF 

        70         80         90        100        110        120 
GHKIPKDQEF QDILTRVKNF PSACPQWLWG SNVRIQVYDP DYMKLILGRS DPKSHHSYRF 

       130        140        150        160        170        180 
LAPWIGYGLL LLNGQTWFQH RRMLTPAFHY DTLKPYVGIM ADSVRIMLDK WEQIVGQDST 

       190        200        210        220        230        240 
LEIFQHITLM TLDTIMKCAF SQEGSVQLDR KYKSYIKAVE DLNNLSFFRI RNIFHQNDII 

       250        260        270        280        290        300 
YSLSSNGRKA RSAWQLAHEH TDQVIKSRKA QLQDEEELQK VKQKRRLDFL DILLFARIEN 

       310        320        330        340        350        360 
GSSLSDKDLR AEVDTFMFEG HDTTASGISW IFYALATNPE HQQGCRKEIQ SLLGDGASIT 

       370        380        390        400        410        420 
WDDLDKMPYT TMCIKEALRI YPPVTAVSRM LSTPVTFPDG RSLPKGITVM LSFYGLHHNP 

       430        440        450        460        470        480 
TVWPNPEVFD PYRFAPESSR HSHSFLPFSG GARNCIGKQF AMNELKVAVA LTLLRFELLP 

       490        500 
DPTRIPIPIP RLVLKSKNGI YLRLKKLQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel member of the cytochrome P450 subfamily IVA in rat prostate." Stroemstedt M., Hayashi S., Zaphiropoulos P.G., Gustafsson J.-A. DNA Cell Biol. 9:569-577(1990) [PubMed: 1980193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Prostate.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Covalently linked heme in cytochrome P4504A fatty acid hydroxylases." Hoch U., Ortiz de Montellano P.R. J. Biol. Chem. 276:11339-11346(2001) [PubMed: 11139583] [Abstract] Cited for: COVALENT HEME ATTACHMENT.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	M37828 mRNA. Translation: AAA63485.1. BC081771 mRNA. Translation: AAH81771.1.
IPI	IPI00214540.
PIR	A36304. PC4352.
RefSeq	NP_113793.2.
UniGene	Rn.10034
3D structure databases
SMR	P24464. Positions 51-507.
ModBase	Search...
Protein-protein interaction databases
STRING	P24464.
Proteomic databases
PRIDE	P24464.
Genome annotation databases
Ensembl	ENSRNOT00000012448; ENSRNOP00000012448; ENSRNOG00000008842; Rattus norvegicus. [Genome view]
GeneID	266674.
KEGG	rno:266674.
NMPDR	fig\|10116.3.peg.23673.
UCSC	NM_031605. rat.
Organism-specific databases
CTD	266674.
RGD	628846. Cyp4a8.
Phylogenomic databases
eggNOG	maNOG05356.
HOVERGEN	P24464.
InParanoid	P24464.
PhylomeDB	P24464.
Enzyme and pathway databases
BRENDA	1.14.14.1. 248.
Gene expression databases
ArrayExpress	P24464.
Genevestigator	P24464.
GermOnline	ENSRNOG00000008842. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	624468.

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Entry information

Entry name

CP4AC_RAT

Accession

Primary (citable) accession number: P24464
Secondary accession number(s): Q642E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 10, 2006
Last modified:	January 19, 2010
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents