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P24464

- CP4AC_RAT

UniProt

P24464 - CP4AC_RAT

Protein

Cytochrome P450 4A12

Gene

Cyp4a12

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei319 – 3191Heme (covalent; via 1 link)
    Metal bindingi455 – 4551Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: UniProtKB

    GO - Biological processi

    1. arachidonic acid metabolic process Source: RGD
    2. fatty acid metabolic process Source: UniProtKB
    3. kidney development Source: RGD
    4. lauric acid metabolic process Source: RGD
    5. response to testosterone Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196408. PPARA activates gene expression.
    REACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 4A12 (EC:1.14.14.1)
    Alternative name(s):
    CYPIVA12
    CYPIVA8
    Cytochrome P450-KP1
    Cytochrome P450-PP1
    Gene namesi
    Name:Cyp4a12
    Synonyms:Cyp4a-8, Cyp4a8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi628846. Cyp4a8.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 508508Cytochrome P450 4A12PRO_0000051818Add
    BLAST

    Proteomic databases

    PaxDbiP24464.
    PRIDEiP24464.

    Expressioni

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Gene expression databases

    GenevestigatoriP24464.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000012448.

    Structurei

    3D structure databases

    ProteinModelPortaliP24464.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00740000115505.
    HOGENOMiHOG000233833.
    HOVERGENiHBG000182.
    InParanoidiP24464.
    KOiK07425.
    OMAiANSACKL.
    OrthoDBiEOG7CNZFK.
    PhylomeDBiP24464.
    TreeFamiTF105088.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24464-1 [UniParc]FASTAAdd to Basket

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    MSGSALSFTI FPGSILGFLQ IATVLTVLLL LFKTAQFYLH RRWLLRATQQ    50
    FPSPPSHWFF GHKIPKDQEF QDILTRVKNF PSACPQWLWG SNVRIQVYDP 100
    DYMKLILGRS DPKSHHSYRF LAPWIGYGLL LLNGQTWFQH RRMLTPAFHY 150
    DTLKPYVGIM ADSVRIMLDK WEQIVGQDST LEIFQHITLM TLDTIMKCAF 200
    SQEGSVQLDR KYKSYIKAVE DLNNLSFFRI RNIFHQNDII YSLSSNGRKA 250
    RSAWQLAHEH TDQVIKSRKA QLQDEEELQK VKQKRRLDFL DILLFARIEN 300
    GSSLSDKDLR AEVDTFMFEG HDTTASGISW IFYALATNPE HQQGCRKEIQ 350
    SLLGDGASIT WDDLDKMPYT TMCIKEALRI YPPVTAVSRM LSTPVTFPDG 400
    RSLPKGITVM LSFYGLHHNP TVWPNPEVFD PYRFAPESSR HSHSFLPFSG 450
    GARNCIGKQF AMNELKVAVA LTLLRFELLP DPTRIPIPIP RLVLKSKNGI 500
    YLRLKKLQ 508
    Length:508
    Mass (Da):58,559
    Last modified:January 10, 2006 - v2
    Checksum:i2C2218525FC65513
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti32 – 321F → L in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti69 – 691E → D in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti101 – 1011D → E in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti114 – 1163SHH → AHG in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti226 – 2261S → F in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti230 – 2334IRNI → VQNM in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti239 – 2391I → F in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti251 – 2522RS → HN in AAA63485. (PubMed:1980193)Curated
    Sequence conflicti259 – 2602EH → DY in AAA63485. (PubMed:1980193)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37828 mRNA. Translation: AAA63485.1.
    BC081771 mRNA. Translation: AAH81771.1.
    PIRiA36304.
    PC4352.
    RefSeqiNP_113793.2. NM_031605.2.
    UniGeneiRn.10034.

    Genome annotation databases

    EnsembliENSRNOT00000012448; ENSRNOP00000012448; ENSRNOG00000008842.
    GeneIDi266674.
    KEGGirno:266674.
    UCSCiRGD:628846. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37828 mRNA. Translation: AAA63485.1 .
    BC081771 mRNA. Translation: AAH81771.1 .
    PIRi A36304.
    PC4352.
    RefSeqi NP_113793.2. NM_031605.2.
    UniGenei Rn.10034.

    3D structure databases

    ProteinModelPortali P24464.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000012448.

    Proteomic databases

    PaxDbi P24464.
    PRIDEi P24464.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012448 ; ENSRNOP00000012448 ; ENSRNOG00000008842 .
    GeneIDi 266674.
    KEGGi rno:266674.
    UCSCi RGD:628846. rat.

    Organism-specific databases

    CTDi 266674.
    RGDi 628846. Cyp4a8.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00740000115505.
    HOGENOMi HOG000233833.
    HOVERGENi HBG000182.
    InParanoidi P24464.
    KOi K07425.
    OMAi ANSACKL.
    OrthoDBi EOG7CNZFK.
    PhylomeDBi P24464.
    TreeFami TF105088.

    Enzyme and pathway databases

    Reactomei REACT_196408. PPARA activates gene expression.
    REACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

    Miscellaneous databases

    NextBioi 624468.
    PROi P24464.

    Gene expression databases

    Genevestigatori P24464.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a novel member of the cytochrome P450 subfamily IVA in rat prostate."
      Stroemstedt M., Hayashi S., Zaphiropoulos P.G., Gustafsson J.-A.
      DNA Cell Biol. 9:569-577(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Prostate.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. "Covalently linked heme in cytochrome P4504A fatty acid hydroxylases."
      Hoch U., Ortiz de Montellano P.R.
      J. Biol. Chem. 276:11339-11346(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: COVALENT HEME ATTACHMENT.

    Entry informationi

    Entry nameiCP4AC_RAT
    AccessioniPrimary (citable) accession number: P24464
    Secondary accession number(s): Q642E9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3