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Protein

Cytochrome P450 4A12

Gene

Cyp4a12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei319 – 3191Heme (covalent; via 1 link)1 Publication
Metal bindingi455 – 4551Iron (heme axial ligand)1 Publication

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. monooxygenase activity Source: UniProtKB

GO - Biological processi

  1. arachidonic acid metabolic process Source: RGD
  2. fatty acid metabolic process Source: UniProtKB
  3. kidney development Source: RGD
  4. lauric acid metabolic process Source: RGD
  5. response to testosterone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A12 (EC:1.14.14.1)
Alternative name(s):
CYPIVA12
CYPIVA8
Cytochrome P450-KP1
Cytochrome P450-PP1
Gene namesi
Name:Cyp4a12
Synonyms:Cyp4a-8, Cyp4a8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi628846. Cyp4a8.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. intracellular membrane-bounded organelle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 508507Cytochrome P450 4A12PRO_0000051818Add
BLAST

Proteomic databases

PaxDbiP24464.
PRIDEiP24464.

Expressioni

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

GenevestigatoriP24464.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012448.

Structurei

3D structure databases

ProteinModelPortaliP24464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP24464.
KOiK07425.
OMAiCKANSAC.
OrthoDBiEOG7CNZFK.
PhylomeDBiP24464.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSALSFTI FPGSILGFLQ IATVLTVLLL LFKTAQFYLH RRWLLRATQQ
60 70 80 90 100
FPSPPSHWFF GHKIPKDQEF QDILTRVKNF PSACPQWLWG SNVRIQVYDP
110 120 130 140 150
DYMKLILGRS DPKSHHSYRF LAPWIGYGLL LLNGQTWFQH RRMLTPAFHY
160 170 180 190 200
DTLKPYVGIM ADSVRIMLDK WEQIVGQDST LEIFQHITLM TLDTIMKCAF
210 220 230 240 250
SQEGSVQLDR KYKSYIKAVE DLNNLSFFRI RNIFHQNDII YSLSSNGRKA
260 270 280 290 300
RSAWQLAHEH TDQVIKSRKA QLQDEEELQK VKQKRRLDFL DILLFARIEN
310 320 330 340 350
GSSLSDKDLR AEVDTFMFEG HDTTASGISW IFYALATNPE HQQGCRKEIQ
360 370 380 390 400
SLLGDGASIT WDDLDKMPYT TMCIKEALRI YPPVTAVSRM LSTPVTFPDG
410 420 430 440 450
RSLPKGITVM LSFYGLHHNP TVWPNPEVFD PYRFAPESSR HSHSFLPFSG
460 470 480 490 500
GARNCIGKQF AMNELKVAVA LTLLRFELLP DPTRIPIPIP RLVLKSKNGI

YLRLKKLQ
Length:508
Mass (Da):58,559
Last modified:January 10, 2006 - v2
Checksum:i2C2218525FC65513
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321F → L in AAA63485 (PubMed:1980193).Curated
Sequence conflicti69 – 691E → D in AAA63485 (PubMed:1980193).Curated
Sequence conflicti101 – 1011D → E in AAA63485 (PubMed:1980193).Curated
Sequence conflicti114 – 1163SHH → AHG in AAA63485 (PubMed:1980193).Curated
Sequence conflicti226 – 2261S → F in AAA63485 (PubMed:1980193).Curated
Sequence conflicti230 – 2334IRNI → VQNM in AAA63485 (PubMed:1980193).Curated
Sequence conflicti239 – 2391I → F in AAA63485 (PubMed:1980193).Curated
Sequence conflicti251 – 2522RS → HN in AAA63485 (PubMed:1980193).Curated
Sequence conflicti259 – 2602EH → DY in AAA63485 (PubMed:1980193).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37828 mRNA. Translation: AAA63485.1.
BC081771 mRNA. Translation: AAH81771.1.
PIRiA36304.
PC4352.
RefSeqiNP_113793.2. NM_031605.2.
UniGeneiRn.10034.

Genome annotation databases

EnsembliENSRNOT00000012448; ENSRNOP00000012448; ENSRNOG00000008842.
GeneIDi266674.
KEGGirno:266674.
UCSCiRGD:628846. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37828 mRNA. Translation: AAA63485.1.
BC081771 mRNA. Translation: AAH81771.1.
PIRiA36304.
PC4352.
RefSeqiNP_113793.2. NM_031605.2.
UniGeneiRn.10034.

3D structure databases

ProteinModelPortaliP24464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012448.

Proteomic databases

PaxDbiP24464.
PRIDEiP24464.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012448; ENSRNOP00000012448; ENSRNOG00000008842.
GeneIDi266674.
KEGGirno:266674.
UCSCiRGD:628846. rat.

Organism-specific databases

CTDi266674.
RGDi628846. Cyp4a8.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP24464.
KOiK07425.
OMAiCKANSAC.
OrthoDBiEOG7CNZFK.
PhylomeDBiP24464.
TreeFamiTF105088.

Miscellaneous databases

NextBioi624468.
PROiP24464.

Gene expression databases

GenevestigatoriP24464.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel member of the cytochrome P450 subfamily IVA in rat prostate."
    Stroemstedt M., Hayashi S., Zaphiropoulos P.G., Gustafsson J.-A.
    DNA Cell Biol. 9:569-577(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Prostate.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Covalently linked heme in cytochrome P4504A fatty acid hydroxylases."
    Hoch U., Ortiz de Montellano P.R.
    J. Biol. Chem. 276:11339-11346(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT.

Entry informationi

Entry nameiCP4AC_RAT
AccessioniPrimary (citable) accession number: P24464
Secondary accession number(s): Q642E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 10, 2006
Last modified: April 1, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.