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P24464 (CP4AC_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 4A12

EC=1.14.14.1
Alternative name(s):
CYPIVA12
CYPIVA8
Cytochrome P450-KP1
Cytochrome P450-PP1
Gene names
Name:Cyp4a12
Synonyms:Cyp4a-8, Cyp4a8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Cytochrome P450 4A12
PRO_0000051818

Sites

Metal binding4551Iron (heme axial ligand)
Binding site3191Heme (covalent; via 1 link)

Experimental info

Sequence conflict321F → L in AAA63485. Ref.1
Sequence conflict691E → D in AAA63485. Ref.1
Sequence conflict1011D → E in AAA63485. Ref.1
Sequence conflict114 – 1163SHH → AHG in AAA63485. Ref.1
Sequence conflict2261S → F in AAA63485. Ref.1
Sequence conflict230 – 2334IRNI → VQNM in AAA63485. Ref.1
Sequence conflict2391I → F in AAA63485. Ref.1
Sequence conflict251 – 2522RS → HN in AAA63485. Ref.1
Sequence conflict259 – 2602EH → DY in AAA63485. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24464 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: 2C2218525FC65513

FASTA50858,559
        10         20         30         40         50         60 
MSGSALSFTI FPGSILGFLQ IATVLTVLLL LFKTAQFYLH RRWLLRATQQ FPSPPSHWFF 

        70         80         90        100        110        120 
GHKIPKDQEF QDILTRVKNF PSACPQWLWG SNVRIQVYDP DYMKLILGRS DPKSHHSYRF 

       130        140        150        160        170        180 
LAPWIGYGLL LLNGQTWFQH RRMLTPAFHY DTLKPYVGIM ADSVRIMLDK WEQIVGQDST 

       190        200        210        220        230        240 
LEIFQHITLM TLDTIMKCAF SQEGSVQLDR KYKSYIKAVE DLNNLSFFRI RNIFHQNDII 

       250        260        270        280        290        300 
YSLSSNGRKA RSAWQLAHEH TDQVIKSRKA QLQDEEELQK VKQKRRLDFL DILLFARIEN 

       310        320        330        340        350        360 
GSSLSDKDLR AEVDTFMFEG HDTTASGISW IFYALATNPE HQQGCRKEIQ SLLGDGASIT 

       370        380        390        400        410        420 
WDDLDKMPYT TMCIKEALRI YPPVTAVSRM LSTPVTFPDG RSLPKGITVM LSFYGLHHNP 

       430        440        450        460        470        480 
TVWPNPEVFD PYRFAPESSR HSHSFLPFSG GARNCIGKQF AMNELKVAVA LTLLRFELLP 

       490        500 
DPTRIPIPIP RLVLKSKNGI YLRLKKLQ 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel member of the cytochrome P450 subfamily IVA in rat prostate."
Stroemstedt M., Hayashi S., Zaphiropoulos P.G., Gustafsson J.-A.
DNA Cell Biol. 9:569-577(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Prostate.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]"Covalently linked heme in cytochrome P4504A fatty acid hydroxylases."
Hoch U., Ortiz de Montellano P.R.
J. Biol. Chem. 276:11339-11346(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: COVALENT HEME ATTACHMENT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M37828 mRNA. Translation: AAA63485.1.
BC081771 mRNA. Translation: AAH81771.1.
PIRA36304.
PC4352.
RefSeqNP_113793.2. NM_031605.2.
UniGeneRn.10034.

3D structure databases

ProteinModelPortalP24464.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000012448.

Proteomic databases

PaxDbP24464.
PRIDEP24464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000012448; ENSRNOP00000012448; ENSRNOG00000008842.
GeneID266674.
KEGGrno:266674.
UCSCRGD:628846. rat.

Organism-specific databases

CTD266674.
RGD628846. Cyp4a8.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00740000115505.
HOGENOMHOG000233833.
HOVERGENHBG000182.
InParanoidP24464.
KOK07425.
OMAANSACKL.
OrthoDBEOG7CNZFK.
PhylomeDBP24464.
TreeFamTF105088.

Gene expression databases

GenevestigatorP24464.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624468.
PROP24464.

Entry information

Entry nameCP4AC_RAT
AccessionPrimary (citable) accession number: P24464
Secondary accession number(s): Q642E9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 10, 2006
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families