P24456 (CP2DA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2D10
EC=1.14.14.1

Alternative name(s):
CYPIID10
Cytochrome P450-16-alpha
Cytochrome P450CB
Testosterone 16-alpha hydroxylase

Gene names

Name:	Cyp2d10
Synonyms:	Cyp2d-10

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Cytochrome P450 2D10		PRO_0000051735
Sites
	Metal binding	446	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	46	1	L → Q in AAA39878. Ref.2
	Sequence conflict	221 – 224	4	GLIP → LAYS in AAA79023. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24456 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 41375F9834489401	FASTA	504	57,233
	10 20 30 40 50 60 MELLTGAGLW SVAIFTVIFI LLVDLMHRHQ RWTSRYPPGP VPWPVLGNLL QVDLDNMPYS 70 80 90 100 110 120 LYKLQNRYGD VFSLQMGWKP MVVINGLKAM KEVLLTCGED TADRPQVPIF EYLGVKPGSQ 130 140 150 160 170 180 GVVLAPYGPE WREQRRFSVS TLRNFGLGKK SLEDWVTKEA RHLCDAFTAQ AGQPINPNTM 190 200 210 220 230 240 LNNAVCNVIA SLIFARRFEY EDPYLIRMQK VLEDSLTEIS GLIPEVLNMF PILLRIPGLP 250 260 270 280 290 300 GKVFQGQKSL LAIVENLLTE NRNTWDPDQP PRNLTDAFLA EIEKVKGNAE SSFNDENLRM 310 320 330 340 350 360 VVLDLFTAGM VTTSTTLSWA LLLMILHPDV QRRVQQEIDA VIGQVRHPEM ADQARMPYTN 370 380 390 400 410 420 AVIHEVQRFG DIAPLNLPRI TSRDIEVQDF LIPKGSILIP NMSSVLKDET VWEKPLRFHP 430 440 450 460 470 480 EHFLDAQGHF VKPEAFMPFS AGRRSCLGEP LARMELFLFF TCLLQHFSFS VPNGQPRPRN 490 500 LGVFPFPVAP YPYQLCAVMR EQGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene family of male-specific testosterone 16 alpha-hydroxylase (C-P-450(16 alpha)) in mice. Organization, differential regulation, and chromosome localization." Wong G., Itakura T., Kawajiri K., Skow L., Negishi M. J. Biol. Chem. 264:2920-2927(1989) [PubMed: 2914938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Functional characterization of two cytochrome P-450s within the mouse, male-specific steroid 16 alpha-hydroxylase gene family: expression in mammalian cells and chimeric proteins." Ichikawa T., Itakura T., Negishi M. Biochemistry 28:4779-4784(1989) [PubMed: 2788458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/J. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Salivary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24263 Genomic DNA. Translation: AAA79023.1. M27167 mRNA. Translation: AAA39878.1. BC010989 mRNA. Translation: AAH10989.1. BC057924 mRNA. Translation: AAH57924.1.
IPI	IPI00323908.
PIR	A30247.
RefSeq	NP_034135.2. NM_010005.3.
UniGene	Mm.174372.
3D structure databases
ProteinModelPortal	P24456.
SMR	P24456. Positions 37-500.
ModBase	Search...
Protein-protein interaction databases
IntAct	P24456. 4 interactions.
STRING	P24456.
Proteomic databases
PRIDE	P24456.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000072776; ENSMUSP00000072555; ENSMUSG00000014372.
GeneID	13101.
KEGG	mmu:13101.
UCSC	uc007wzf.2. mouse.
Organism-specific databases
CTD	13101.
MGI	MGI:88602. Cyp2d10.
Phylogenomic databases
eggNOG	roNOG04886.
GeneTree	ENSGT00560000076621.
HOGENOM	HBG749920.
HOVERGEN	HBG015789.
InParanoid	P24456.
OMA	VVESETN.
PhylomeDB	P24456.
Gene expression databases
ArrayExpress	P24456.
Bgee	P24456.
Genevestigator	P24456.
GermOnline	ENSMUSG00000014372. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K07414.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	283094.
SOURCE	Search...

Entry information

Entry name

CP2DA_MOUSE

Accession

Primary (citable) accession number: P24456
Secondary accession number(s): Q64490

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 15, 1998
Last modified:	November 16, 2011
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program