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P24455

- CP2A9_MESAU

UniProt

P24455 - CP2A9_MESAU

Protein

Cytochrome P450 2A9

Gene

CYP2A9

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi438 – 4381Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A9 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIA9
    Cytochrome P450-MC1-R
    Testosterone 7-alpha-hydroxylase
    Gene namesi
    Name:CYP2A9
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493Cytochrome P450 2A9PRO_0000051672Add
    BLAST

    Expressioni

    Tissue specificityi

    Liver.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4996550.

    Structurei

    3D structure databases

    ProteinModelPortaliP24455.
    SMRiP24455. Positions 30-492.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    HOVERGENiHBG015789.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24455-1 [UniParc]FASTAAdd to Basket

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    MLGSGLILVA ILAYLSVMVL VFVWKQKFRG KLPPGPTPLP YIGNYLQLNT    50
    KDIYSSITEL SERYGPVFTI YLGPRPVVVL YGYDAVKEAL VDQAEEFSGR 100
    GEQATYNTLF KDYGVAFSSG ERAKQLRRFS IATLRDFGVG KRGVEERIQE 150
    EAAYLIKMLR STRGAPIDPN DYLSQTVSNV ISSVVFGDAF DYEDKEFLEL 200
    LHMMNEMNKF AASPVGQLYD MFHSVMKYLP GPQQQIIKNT KELEDFMIRK 250
    VKQNQSTLDL NSARNFIDSF LIHMHEEKKN PTSEFNIKNL VMTSLNLFFA 300
    GSETVSSTIR YGFLLLMKYP EVEAKVHEEI DRVIGRNRQP QFEDRMKMPY 350
    TEAVINEIQR FANLAPLGIP RKTIKNTTFR GFFLPKDTDV YPILGSLLTD 400
    PKFFTSPKHF NPQNFLDDRG QLKKIAAFVP FSVGKRFCLG DGLARMELFL 450
    FLTTILQNFR LKFPKKLEDI DASPKPLGFS RIIPRYTMSF LPI 493
    Length:493
    Mass (Da):56,418
    Last modified:December 15, 1998 - v2
    Checksum:iE7376475D21AEC10
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti367 – 3671L → C in AAA37069. (PubMed:2275554)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86953 mRNA. Translation: BAA25259.1.
    M63789 mRNA. Translation: AAA37069.1.
    RefSeqiNP_001268294.1. NM_001281365.1.

    Genome annotation databases

    GeneIDi101826326.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86953 mRNA. Translation: BAA25259.1 .
    M63789 mRNA. Translation: AAA37069.1 .
    RefSeqi NP_001268294.1. NM_001281365.1.

    3D structure databases

    ProteinModelPortali P24455.
    SMRi P24455. Positions 30-492.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4996550.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101826326.

    Phylogenomic databases

    HOVERGENi HBG015789.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of Syrian hamster testosterone 7 alpha-hydroxylase, CYP2A9."
      Kurose K., Tohkin M., Ushio F., Fukuhara M.
      Arch. Biochem. Biophys. 351:60-65(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s."
      Lai T.S., Chiang J.Y.L.
      Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 208-493.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP2A9_MESAU
    AccessioniPrimary (citable) accession number: P24455
    Secondary accession number(s): O70538
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3