P24455 (CP2A9_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2A9
EC=1.14.14.1

Alternative name(s):
CYPIIA9
Cytochrome P450-MC1-R
Testosterone 7-alpha-hydroxylase

Gene names

Name:

CYP2A9

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Cytochrome P450 2A9		PRO_0000051672
Sites
	Metal binding	438	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	367	1	L → C in AAA37069. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24455 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: E7376475D21AEC10	FASTA	493	56,418
	10 20 30 40 50 60 MLGSGLILVA ILAYLSVMVL VFVWKQKFRG KLPPGPTPLP YIGNYLQLNT KDIYSSITEL 70 80 90 100 110 120 SERYGPVFTI YLGPRPVVVL YGYDAVKEAL VDQAEEFSGR GEQATYNTLF KDYGVAFSSG 130 140 150 160 170 180 ERAKQLRRFS IATLRDFGVG KRGVEERIQE EAAYLIKMLR STRGAPIDPN DYLSQTVSNV 190 200 210 220 230 240 ISSVVFGDAF DYEDKEFLEL LHMMNEMNKF AASPVGQLYD MFHSVMKYLP GPQQQIIKNT 250 260 270 280 290 300 KELEDFMIRK VKQNQSTLDL NSARNFIDSF LIHMHEEKKN PTSEFNIKNL VMTSLNLFFA 310 320 330 340 350 360 GSETVSSTIR YGFLLLMKYP EVEAKVHEEI DRVIGRNRQP QFEDRMKMPY TEAVINEIQR 370 380 390 400 410 420 FANLAPLGIP RKTIKNTTFR GFFLPKDTDV YPILGSLLTD PKFFTSPKHF NPQNFLDDRG 430 440 450 460 470 480 QLKKIAAFVP FSVGKRFCLG DGLARMELFL FLTTILQNFR LKFPKKLEDI DASPKPLGFS 490 RIIPRYTMSF LPI

References

[1]	"Cloning and characterization of Syrian hamster testosterone 7 alpha-hydroxylase, CYP2A9." Kurose K., Tohkin M., Ushio F., Fukuhara M. Arch. Biochem. Biophys. 351:60-65(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s." Lai T.S., Chiang J.Y.L. Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 208-493. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86953 mRNA. Translation: BAA25259.1. M63789 mRNA. Translation: AAA37069.1.
3D structure databases
ProteinModelPortal	P24455.
SMR	P24455. Positions 30-492.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2A9_MESAU

Accession

Primary (citable) accession number: P24455
Secondary accession number(s): O70538

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 15, 1998
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program