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P24454

- CP2A8_MESAU

UniProt

P24454 - CP2A8_MESAU

Protein

Cytochrome P450 2A8

Gene

CYP2A8

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Highly active in 7-ethoxycoumarin O-deethylation, and benzphetamine N-demethylation; moderately active in testosterone 7-alpha-hydroxylation, ethylmorphine N-demethylation, p-nitroanisole O-demethylation; and only slightly active in benzopyrene 3-hydroxylation, 7-ethoxyresorufin O-deethylation, testosterone 2-alpha-hydroxylation and testosterone 17-oxidation. Competent in the metabolic activation of aflatoxin B1.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A8 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIA8
    Cytochrome P450-AFB
    Cytochrome P450-MC1
    Gene namesi
    Name:CYP2A8
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Cytochrome P450 2A8PRO_0000051671Add
    BLAST

    Expressioni

    Tissue specificityi

    Liver.

    Inductioni

    By 3-methylcholanthrene (3MC).

    Structurei

    3D structure databases

    ProteinModelPortaliP24454.
    SMRiP24454. Positions 32-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    HOVERGENiHBG015789.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24454-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVSGMLLVV VLTCLSVMII MSVWRQRRLL RKMPPGPTPL PFIGNFLELD    50
    TEKFYDCLSK MRERYGPVFT IHLGPRPAVM LWGYDAVKEA LIDQAEELSD 100
    RGEQAFFDWF FKGYGVVFSS GERAKQLRRF SIATLRDFGF GKRGIEERTI 150
    EETSFLIQAL RDTNGATIDP TFYMSRTVSN VISSIVFGNR FEYDDKEFLS 200
    LLGMIMRSFQ FMSTSTGQLF EMFYSVMKHL PGCQHQAYKE MQGLEDFIAR 250
    KVEENQRTLD PNSPRDFIDS FLIRMQEEKK NPRTQFHMRN LLMTTLNLFF 300
    AGTETVSTTT RYGFLLLMKY PHIAAKMHEE IDQVIGRNRQ PKYEDHLKMP 350
    YTEAVIYEIQ RFVDVVPLGL PRSTTKDIKF RDFLIPKGTD VFPVLSSVLK 400
    DPKFFSNPND FNPQHFSDDK GQFKKSNAFM PFSVGKRYCF GESLAKMELF 450
    IFFTTIMQNF CFKSPQAPQD IDVTPQYFSF AAIPPKFTMS FLPR 494
    Length:494
    Mass (Da):57,387
    Last modified:March 1, 1992 - v1
    Checksum:i16816672AA4B112B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti417 – 4171S → L in AAA37084. (PubMed:2502109)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63788 mRNA. Translation: AAA37068.1.
    M27906 mRNA. Translation: AAA37084.1.
    PIRiA33293.
    RefSeqiNP_001268512.1. NM_001281583.1.

    Genome annotation databases

    GeneIDi101825889.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63788 mRNA. Translation: AAA37068.1 .
    M27906 mRNA. Translation: AAA37084.1 .
    PIRi A33293.
    RefSeqi NP_001268512.1. NM_001281583.1.

    3D structure databases

    ProteinModelPortali P24454.
    SMRi P24454. Positions 32-494.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101825889.

    Phylogenomic databases

    HOVERGENi HBG015789.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s."
      Lai T.S., Chiang J.Y.L.
      Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Complete cDNA sequence of a major 3-methylcholanthrene-inducible cytochrome P-450 isozyme (P-450AFB) of Syrian hamsters with high activity toward aflatoxin B1."
      Fukuhara M., Nagata K., Mizokami K., Yamazoe Y., Takanaka A., Kato R.
      Biochem. Biophys. Res. Commun. 162:265-272(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver."
      Koga N., Ariyoshi N., Nakashima H., Yoshimura H.
      J. Biochem. 107:826-833(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP2A8_MESAU
    AccessioniPrimary (citable) accession number: P24454
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3