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P24454 (CP2A8_MESAU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2A8

EC=1.14.14.1
Alternative name(s):
CYPIIA8
Cytochrome P450-AFB
Cytochrome P450-MC1
Gene names
Name:CYP2A8
OrganismMesocricetus auratus (Golden hamster)
Taxonomic identifier10036 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Highly active in 7-ethoxycoumarin O-deethylation, and benzphetamine N-demethylation; moderately active in testosterone 7-alpha-hydroxylation, ethylmorphine N-demethylation, p-nitroanisole O-demethylation; and only slightly active in benzopyrene 3-hydroxylation, 7-ethoxyresorufin O-deethylation, testosterone 2-alpha-hydroxylation and testosterone 17-oxidation. Competent in the metabolic activation of aflatoxin B1.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver.

Induction

By 3-methylcholanthrene (3MC).

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Cytochrome P450 2A8
PRO_0000051671

Sites

Metal binding4391Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict4171S → L in AAA37084. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P24454 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 16816672AA4B112B

FASTA49457,387
        10         20         30         40         50         60 
MLVSGMLLVV VLTCLSVMII MSVWRQRRLL RKMPPGPTPL PFIGNFLELD TEKFYDCLSK 

        70         80         90        100        110        120 
MRERYGPVFT IHLGPRPAVM LWGYDAVKEA LIDQAEELSD RGEQAFFDWF FKGYGVVFSS 

       130        140        150        160        170        180 
GERAKQLRRF SIATLRDFGF GKRGIEERTI EETSFLIQAL RDTNGATIDP TFYMSRTVSN 

       190        200        210        220        230        240 
VISSIVFGNR FEYDDKEFLS LLGMIMRSFQ FMSTSTGQLF EMFYSVMKHL PGCQHQAYKE 

       250        260        270        280        290        300 
MQGLEDFIAR KVEENQRTLD PNSPRDFIDS FLIRMQEEKK NPRTQFHMRN LLMTTLNLFF 

       310        320        330        340        350        360 
AGTETVSTTT RYGFLLLMKY PHIAAKMHEE IDQVIGRNRQ PKYEDHLKMP YTEAVIYEIQ 

       370        380        390        400        410        420 
RFVDVVPLGL PRSTTKDIKF RDFLIPKGTD VFPVLSSVLK DPKFFSNPND FNPQHFSDDK 

       430        440        450        460        470        480 
GQFKKSNAFM PFSVGKRYCF GESLAKMELF IFFTTIMQNF CFKSPQAPQD IDVTPQYFSF 

       490 
AAIPPKFTMS FLPR 

« Hide

References

[1]"Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s."
Lai T.S., Chiang J.Y.L.
Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Complete cDNA sequence of a major 3-methylcholanthrene-inducible cytochrome P-450 isozyme (P-450AFB) of Syrian hamsters with high activity toward aflatoxin B1."
Fukuhara M., Nagata K., Mizokami K., Yamazoe Y., Takanaka A., Kato R.
Biochem. Biophys. Res. Commun. 162:265-272(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver."
Koga N., Ariyoshi N., Nakashima H., Yoshimura H.
J. Biochem. 107:826-833(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63788 mRNA. Translation: AAA37068.1.
M27906 mRNA. Translation: AAA37084.1.
PIRA33293.
RefSeqNP_001268512.1. NM_001281583.1.

3D structure databases

ProteinModelPortalP24454.
SMRP24454. Positions 32-494.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101825889.

Phylogenomic databases

HOVERGENHBG015789.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP2A8_MESAU
AccessionPrimary (citable) accession number: P24454
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: December 11, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families