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Protein

Cytochrome P450 2A8

Gene

CYP2A8

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Highly active in 7-ethoxycoumarin O-deethylation, and benzphetamine N-demethylation; moderately active in testosterone 7-alpha-hydroxylation, ethylmorphine N-demethylation, p-nitroanisole O-demethylation; and only slightly active in benzopyrene 3-hydroxylation, 7-ethoxyresorufin O-deethylation, testosterone 2-alpha-hydroxylation and testosterone 17-oxidation. Competent in the metabolic activation of aflatoxin B1.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A8 (EC:1.14.14.1)
Alternative name(s):
CYPIIA8
Cytochrome P450-AFB
Cytochrome P450-MC1
Gene namesi
Name:CYP2A8
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Cytochrome P450 2A8PRO_0000051671Add
BLAST

Expressioni

Tissue specificityi

Liver.

Inductioni

By 3-methylcholanthrene (3MC).

Structurei

3D structure databases

ProteinModelPortaliP24454.
SMRiP24454. Positions 32-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG015789.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVSGMLLVV VLTCLSVMII MSVWRQRRLL RKMPPGPTPL PFIGNFLELD
60 70 80 90 100
TEKFYDCLSK MRERYGPVFT IHLGPRPAVM LWGYDAVKEA LIDQAEELSD
110 120 130 140 150
RGEQAFFDWF FKGYGVVFSS GERAKQLRRF SIATLRDFGF GKRGIEERTI
160 170 180 190 200
EETSFLIQAL RDTNGATIDP TFYMSRTVSN VISSIVFGNR FEYDDKEFLS
210 220 230 240 250
LLGMIMRSFQ FMSTSTGQLF EMFYSVMKHL PGCQHQAYKE MQGLEDFIAR
260 270 280 290 300
KVEENQRTLD PNSPRDFIDS FLIRMQEEKK NPRTQFHMRN LLMTTLNLFF
310 320 330 340 350
AGTETVSTTT RYGFLLLMKY PHIAAKMHEE IDQVIGRNRQ PKYEDHLKMP
360 370 380 390 400
YTEAVIYEIQ RFVDVVPLGL PRSTTKDIKF RDFLIPKGTD VFPVLSSVLK
410 420 430 440 450
DPKFFSNPND FNPQHFSDDK GQFKKSNAFM PFSVGKRYCF GESLAKMELF
460 470 480 490
IFFTTIMQNF CFKSPQAPQD IDVTPQYFSF AAIPPKFTMS FLPR
Length:494
Mass (Da):57,387
Last modified:March 1, 1992 - v1
Checksum:i16816672AA4B112B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti417 – 4171S → L in AAA37084 (PubMed:2502109).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63788 mRNA. Translation: AAA37068.1.
M27906 mRNA. Translation: AAA37084.1.
PIRiA33293.
RefSeqiNP_001268512.1. NM_001281583.1.

Genome annotation databases

GeneIDi101825889.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63788 mRNA. Translation: AAA37068.1.
M27906 mRNA. Translation: AAA37084.1.
PIRiA33293.
RefSeqiNP_001268512.1. NM_001281583.1.

3D structure databases

ProteinModelPortaliP24454.
SMRiP24454. Positions 32-494.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101825889.

Phylogenomic databases

HOVERGENiHBG015789.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s."
    Lai T.S., Chiang J.Y.L.
    Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Complete cDNA sequence of a major 3-methylcholanthrene-inducible cytochrome P-450 isozyme (P-450AFB) of Syrian hamsters with high activity toward aflatoxin B1."
    Fukuhara M., Nagata K., Mizokami K., Yamazoe Y., Takanaka A., Kato R.
    Biochem. Biophys. Res. Commun. 162:265-272(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver."
    Koga N., Ariyoshi N., Nakashima H., Yoshimura H.
    J. Biochem. 107:826-833(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15.
    Tissue: Liver.

Entry informationi

Entry nameiCP2A8_MESAU
AccessioniPrimary (citable) accession number: P24454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.