P24454 (CP2A8_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2A8
EC=1.14.14.1

Alternative name(s):
CYPIIA8
Cytochrome P450-AFB
Cytochrome P450-MC1

Gene names

Name:

CYP2A8

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Highly active in 7-ethoxycoumarin O-deethylation, and benzphetamine N-demethylation; moderately active in testosterone 7-alpha-hydroxylation, ethylmorphine N-demethylation, p-nitroanisole O-demethylation; and only slightly active in benzopyrene 3-hydroxylation, 7-ethoxyresorufin O-deethylation, testosterone 2-alpha-hydroxylation and testosterone 17-oxidation. Competent in the metabolic activation of aflatoxin B1.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Cytochrome P450 2A8		PRO_0000051671
Sites
	Metal binding	439	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	417	1	S → L in AAA37084. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24454 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 16816672AA4B112B	FASTA	494	57,387
	10 20 30 40 50 60 MLVSGMLLVV VLTCLSVMII MSVWRQRRLL RKMPPGPTPL PFIGNFLELD TEKFYDCLSK 70 80 90 100 110 120 MRERYGPVFT IHLGPRPAVM LWGYDAVKEA LIDQAEELSD RGEQAFFDWF FKGYGVVFSS 130 140 150 160 170 180 GERAKQLRRF SIATLRDFGF GKRGIEERTI EETSFLIQAL RDTNGATIDP TFYMSRTVSN 190 200 210 220 230 240 VISSIVFGNR FEYDDKEFLS LLGMIMRSFQ FMSTSTGQLF EMFYSVMKHL PGCQHQAYKE 250 260 270 280 290 300 MQGLEDFIAR KVEENQRTLD PNSPRDFIDS FLIRMQEEKK NPRTQFHMRN LLMTTLNLFF 310 320 330 340 350 360 AGTETVSTTT RYGFLLLMKY PHIAAKMHEE IDQVIGRNRQ PKYEDHLKMP YTEAVIYEIQ 370 380 390 400 410 420 RFVDVVPLGL PRSTTKDIKF RDFLIPKGTD VFPVLSSVLK DPKFFSNPND FNPQHFSDDK 430 440 450 460 470 480 GQFKKSNAFM PFSVGKRYCF GESLAKMELF IFFTTIMQNF CFKSPQAPQD IDVTPQYFSF 490 AAIPPKFTMS FLPR

References

[1]	"Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s." Lai T.S., Chiang J.Y.L. Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Complete cDNA sequence of a major 3-methylcholanthrene-inducible cytochrome P-450 isozyme (P-450AFB) of Syrian hamsters with high activity toward aflatoxin B1." Fukuhara M., Nagata K., Mizokami K., Yamazoe Y., Takanaka A., Kato R. Biochem. Biophys. Res. Commun. 162:265-272(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver." Koga N., Ariyoshi N., Nakashima H., Yoshimura H. J. Biochem. 107:826-833(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63788 mRNA. Translation: AAA37068.1. M27906 mRNA. Translation: AAA37084.1.
PIR	A33293.
3D structure databases
ProteinModelPortal	P24454.
SMR	P24454. Positions 32-494.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2A8_MESAU

Accession

Primary (citable) accession number: P24454

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program