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P24453 (CP1A2_MESAU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 1A2

EC=1.14.14.1
Alternative name(s):
CYPIA2
Cytochrome P450-MC4
Hepatic cytochrome P-450MC1
Gene names
Name:CYP1A2
OrganismMesocricetus auratus (Golden hamster)
Taxonomic identifier10036 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Found in lung and liver.

Induction

By 3-methylcholanthrene (3MC).

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 513512Cytochrome P450 1A2
PRO_0000051653

Sites

Metal binding4561Iron (heme axial ligand) By similarity
Binding site2251Substrate By similarity

Experimental info

Sequence conflict491I → F in AAA37070. Ref.1
Sequence conflict52 – 532HV → MC in AAA37070. Ref.1
Sequence conflict253 – 2542KN → GG in AAA37070. Ref.1
Sequence conflict3261L → W in AAA37070. Ref.1
Sequence conflict3561R → L in AAA37070. Ref.1
Sequence conflict4851T → Q in AAA37070. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24453 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: D179B43386C19815

FASTA51358,082
        10         20         30         40         50         60 
MALSQYTSLS TELVLATAIF CIVFWVARAL RTQVPKGLKT PPGPWGLPIL GHVLTLGKNP 

        70         80         90        100        110        120 
HLSLTKLSKQ YGDVLQIRIG STPVVVLSGL DTIRQALVRQ GDDFKGRPDL YSFTLITNGK 

       130        140        150        160        170        180 
SMTFNPDCGP VWAARRRLAQ DALKSFSIAS DPTSASSCYL EDHVIKEANH LVSKLQKLTA 

       190        200        210        220        230        240 
EVGHFEPVNQ VVESVANVIG AMCFGKNFPR KSEEMLRIVK GSSDFVENVS SGNAVDFFPI 

       250        260        270        280        290        300 
LRYLPNPDLK RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENSKDSGGLI 

       310        320        330        340        350        360 
PQEKIVNIVN DLFGAGFDTV TTAITLSILL LVTWPNVQRK IHKELDTVIG RDRQPRLSDR 

       370        380        390        400        410        420 
LQLPYMEAFI LELYRYTSFV PFTIPHSTTR DTSLNGFYIP KDRCIFINQW QVNHDEKQWK 

       430        440        450        460        470        480 
DPFVFRPERF LTDNDTVINK TLSEKVMLFG LGKRRCIGEI PAKWEVFLFL AILLQQLEFS 

       490        500        510 
VPPGTKVDLT PTYGLTMKPQ TCKYIQAWPR FSK 

« Hide

References

[1]"Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s."
Lai T.S., Chiang J.Y.L.
Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in lung and liver: cDNA cloning and sequence analysis."
Sagami I., Ohmachi T., Fujii H., Kikuchi H., Watanabe M.
J. Biochem. 110:641-647(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver."
Koga N., Ariyoshi N., Nakashima H., Yoshimura H.
J. Biochem. 107:826-833(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-19.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63787 mRNA. Translation: AAA37070.1.
D10252 mRNA. Translation: BAA01097.1.
D10914 Genomic DNA. Translation: BAA01718.1.
PIRJX0190.
RefSeqNP_001268302.1. NM_001281373.1.

3D structure databases

ProteinModelPortalP24453.
SMRP24453. Positions 33-512.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101825565.

Phylogenomic databases

HOVERGENHBG106944.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP1A2_MESAU
AccessionPrimary (citable) accession number: P24453
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: December 11, 2013
This is version 90 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families