P24453 (CP1A2_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 1A2
EC=1.14.14.1

Alternative name(s):
CYPIA2
Cytochrome P450-MC4
Hepatic cytochrome P-450MC1

Gene names

Name:

CYP1A2

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Found in lung and liver.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 513	512	Cytochrome P450 1A2		PRO_0000051653
Sites
	Metal binding	456	1	Iron (heme axial ligand) By similarity
	Binding site	225	1	Substrate By similarity
Amino acid modifications
	Modified residue	288	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	49	1	I → F in AAA37070. Ref.1
	Sequence conflict	52 – 53	2	HV → MC in AAA37070. Ref.1
	Sequence conflict	253 – 254	2	KN → GG in AAA37070. Ref.1
	Sequence conflict	326	1	L → W in AAA37070. Ref.1
	Sequence conflict	356	1	R → L in AAA37070. Ref.1
	Sequence conflict	485	1	T → Q in AAA37070. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24453 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: D179B43386C19815	FASTA	513	58,082
	10 20 30 40 50 60 MALSQYTSLS TELVLATAIF CIVFWVARAL RTQVPKGLKT PPGPWGLPIL GHVLTLGKNP 70 80 90 100 110 120 HLSLTKLSKQ YGDVLQIRIG STPVVVLSGL DTIRQALVRQ GDDFKGRPDL YSFTLITNGK 130 140 150 160 170 180 SMTFNPDCGP VWAARRRLAQ DALKSFSIAS DPTSASSCYL EDHVIKEANH LVSKLQKLTA 190 200 210 220 230 240 EVGHFEPVNQ VVESVANVIG AMCFGKNFPR KSEEMLRIVK GSSDFVENVS SGNAVDFFPI 250 260 270 280 290 300 LRYLPNPDLK RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENSKDSGGLI 310 320 330 340 350 360 PQEKIVNIVN DLFGAGFDTV TTAITLSILL LVTWPNVQRK IHKELDTVIG RDRQPRLSDR 370 380 390 400 410 420 LQLPYMEAFI LELYRYTSFV PFTIPHSTTR DTSLNGFYIP KDRCIFINQW QVNHDEKQWK 430 440 450 460 470 480 DPFVFRPERF LTDNDTVINK TLSEKVMLFG LGKRRCIGEI PAKWEVFLFL AILLQQLEFS 490 500 510 VPPGTKVDLT PTYGLTMKPQ TCKYIQAWPR FSK

References

[1]	"Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s." Lai T.S., Chiang J.Y.L. Arch. Biochem. Biophys. 283:429-439(1990) [PubMed: 2275554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in lung and liver: cDNA cloning and sequence analysis." Sagami I., Ohmachi T., Fujii H., Kikuchi H., Watanabe M. J. Biochem. 110:641-647(1991) [PubMed: 1778988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver." Koga N., Ariyoshi N., Nakashima H., Yoshimura H. J. Biochem. 107:826-833(1990) [PubMed: 2391346] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-19. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63787 mRNA. Translation: AAA37070.1. D10252 mRNA. Translation: BAA01097.1. D10914 Genomic DNA. Translation: BAA01718.1.
PIR	JX0190.
3D structure databases
ProteinModelPortal	P24453.
SMR	P24453. Positions 33-512.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG106944.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A2_MESAU

Accession

Primary (citable) accession number: P24453

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 28, 2011
This is version 82 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program