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P24453

- CP1A2_MESAU

UniProt

P24453 - CP1A2_MESAU

Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.By similarity

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251SubstrateBy similarity
    Metal bindingi456 – 4561Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 1A2 (EC:1.14.14.1)
    Alternative name(s):
    CYPIA2
    Cytochrome P450-MC4
    Hepatic cytochrome P-450MC1
    Gene namesi
    Name:CYP1A2
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 513512Cytochrome P450 1A2PRO_0000051653Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi68 – 681O-linked (GlcNAc)By similarity

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Found in lung and liver.

    Inductioni

    By 3-methylcholanthrene (3MC).

    Structurei

    3D structure databases

    ProteinModelPortaliP24453.
    SMRiP24453. Positions 33-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    HOVERGENiHBG106944.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24453-1 [UniParc]FASTAAdd to Basket

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    MALSQYTSLS TELVLATAIF CIVFWVARAL RTQVPKGLKT PPGPWGLPIL    50
    GHVLTLGKNP HLSLTKLSKQ YGDVLQIRIG STPVVVLSGL DTIRQALVRQ 100
    GDDFKGRPDL YSFTLITNGK SMTFNPDCGP VWAARRRLAQ DALKSFSIAS 150
    DPTSASSCYL EDHVIKEANH LVSKLQKLTA EVGHFEPVNQ VVESVANVIG 200
    AMCFGKNFPR KSEEMLRIVK GSSDFVENVS SGNAVDFFPI LRYLPNPDLK 250
    RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENSKDSGGLI 300
    PQEKIVNIVN DLFGAGFDTV TTAITLSILL LVTWPNVQRK IHKELDTVIG 350
    RDRQPRLSDR LQLPYMEAFI LELYRYTSFV PFTIPHSTTR DTSLNGFYIP 400
    KDRCIFINQW QVNHDEKQWK DPFVFRPERF LTDNDTVINK TLSEKVMLFG 450
    LGKRRCIGEI PAKWEVFLFL AILLQQLEFS VPPGTKVDLT PTYGLTMKPQ 500
    TCKYIQAWPR FSK 513
    Length:513
    Mass (Da):58,082
    Last modified:January 23, 2007 - v4
    Checksum:iD179B43386C19815
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491I → F in AAA37070. (PubMed:2275554)Curated
    Sequence conflicti52 – 532HV → MC in AAA37070. (PubMed:2275554)Curated
    Sequence conflicti253 – 2542KN → GG in AAA37070. (PubMed:2275554)Curated
    Sequence conflicti326 – 3261L → W in AAA37070. (PubMed:2275554)Curated
    Sequence conflicti356 – 3561R → L in AAA37070. (PubMed:2275554)Curated
    Sequence conflicti485 – 4851T → Q in AAA37070. (PubMed:2275554)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63787 mRNA. Translation: AAA37070.1.
    D10252 mRNA. Translation: BAA01097.1.
    D10914 Genomic DNA. Translation: BAA01718.1.
    PIRiJX0190.
    RefSeqiNP_001268302.1. NM_001281373.1.

    Genome annotation databases

    GeneIDi101825565.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63787 mRNA. Translation: AAA37070.1 .
    D10252 mRNA. Translation: BAA01097.1 .
    D10914 Genomic DNA. Translation: BAA01718.1 .
    PIRi JX0190.
    RefSeqi NP_001268302.1. NM_001281373.1.

    3D structure databases

    ProteinModelPortali P24453.
    SMRi P24453. Positions 33-512.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101825565.

    Phylogenomic databases

    HOVERGENi HBG106944.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s."
      Lai T.S., Chiang J.Y.L.
      Arch. Biochem. Biophys. 283:429-439(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in lung and liver: cDNA cloning and sequence analysis."
      Sagami I., Ohmachi T., Fujii H., Kikuchi H., Watanabe M.
      J. Biochem. 110:641-647(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver."
      Koga N., Ariyoshi N., Nakashima H., Yoshimura H.
      J. Biochem. 107:826-833(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-19.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP1A2_MESAU
    AccessioniPrimary (citable) accession number: P24453
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3