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Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.By similarity

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225SubstrateBy similarity1
Metal bindingi456Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1By similarity)
Alternative name(s):
CYPIA2
Cholesterol 25-hydroxylaseBy similarity
Cytochrome P450-MC4
Hepatic cytochrome P-450MC1
Gene namesi
Name:CYP1A2
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000516532 – 513Cytochrome P450 1A2Add BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68O-linked (GlcNAc)By similarity1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Found in lung and liver.

Inductioni

By 3-methylcholanthrene (3MC).

Structurei

3D structure databases

ProteinModelPortaliP24453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.
OrthoDBiEOG091G0BT8.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSQYTSLS TELVLATAIF CIVFWVARAL RTQVPKGLKT PPGPWGLPIL
60 70 80 90 100
GHVLTLGKNP HLSLTKLSKQ YGDVLQIRIG STPVVVLSGL DTIRQALVRQ
110 120 130 140 150
GDDFKGRPDL YSFTLITNGK SMTFNPDCGP VWAARRRLAQ DALKSFSIAS
160 170 180 190 200
DPTSASSCYL EDHVIKEANH LVSKLQKLTA EVGHFEPVNQ VVESVANVIG
210 220 230 240 250
AMCFGKNFPR KSEEMLRIVK GSSDFVENVS SGNAVDFFPI LRYLPNPDLK
260 270 280 290 300
RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENSKDSGGLI
310 320 330 340 350
PQEKIVNIVN DLFGAGFDTV TTAITLSILL LVTWPNVQRK IHKELDTVIG
360 370 380 390 400
RDRQPRLSDR LQLPYMEAFI LELYRYTSFV PFTIPHSTTR DTSLNGFYIP
410 420 430 440 450
KDRCIFINQW QVNHDEKQWK DPFVFRPERF LTDNDTVINK TLSEKVMLFG
460 470 480 490 500
LGKRRCIGEI PAKWEVFLFL AILLQQLEFS VPPGTKVDLT PTYGLTMKPQ
510
TCKYIQAWPR FSK
Length:513
Mass (Da):58,082
Last modified:January 23, 2007 - v4
Checksum:iD179B43386C19815
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49I → F in AAA37070 (PubMed:2275554).Curated1
Sequence conflicti52 – 53HV → MC in AAA37070 (PubMed:2275554).Curated2
Sequence conflicti253 – 254KN → GG in AAA37070 (PubMed:2275554).Curated2
Sequence conflicti326L → W in AAA37070 (PubMed:2275554).Curated1
Sequence conflicti356R → L in AAA37070 (PubMed:2275554).Curated1
Sequence conflicti485T → Q in AAA37070 (PubMed:2275554).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63787 mRNA. Translation: AAA37070.1.
D10252 mRNA. Translation: BAA01097.1.
D10914 Genomic DNA. Translation: BAA01718.1.
PIRiJX0190.
RefSeqiNP_001268302.1. NM_001281373.1.

Genome annotation databases

GeneIDi101825565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63787 mRNA. Translation: AAA37070.1.
D10252 mRNA. Translation: BAA01097.1.
D10914 Genomic DNA. Translation: BAA01718.1.
PIRiJX0190.
RefSeqiNP_001268302.1. NM_001281373.1.

3D structure databases

ProteinModelPortaliP24453.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101825565.

Phylogenomic databases

HOVERGENiHBG106944.
OrthoDBiEOG091G0BT8.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP1A2_MESAU
AccessioniPrimary (citable) accession number: P24453
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.