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P24425

- KITH_EHV4

UniProt

P24425 - KITH_EHV4

Protein

Thymidine kinase

Gene

TK

Organism
Equine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei60 – 601Proton acceptorSequence Analysis
    Binding sitei78 – 781Substrate
    Binding sitei102 – 1021Substrate
    Binding sitei105 – 1051Substrate
    Binding sitei148 – 1481Substrate
    Binding sitei192 – 1921ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 398ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. TMP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    OrganismiEquine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
    Taxonomic identifieri10333 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiEquus caballus (Horse) [TaxID: 9796]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 352352Thymidine kinasePRO_0000175067Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1
    352
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi23 – 319
    Helixi38 – 469
    Helixi48 – 503
    Beta strandi55 – 584
    Helixi62 – 665
    Beta strandi68 – 714
    Helixi73 – 8614
    Helixi91 – 11727
    Beta strandi122 – 1254
    Beta strandi132 – 1398
    Helixi142 – 1454
    Helixi147 – 1548
    Helixi160 – 1678
    Beta strandi177 – 1837
    Helixi186 – 19611
    Helixi205 – 22622
    Turni231 – 2377
    Helixi243 – 2497
    Helixi263 – 2653
    Helixi267 – 2715
    Helixi274 – 2763
    Helixi285 – 29814
    Beta strandi301 – 3077
    Helixi312 – 3209
    Helixi321 – 3255
    Beta strandi328 – 3325
    Helixi333 – 35018

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1P6XX-ray2.00A/B23-352[»]
    1P72X-ray2.10A/B23-352[»]
    1P73X-ray2.70A/B/C/D23-352[»]
    1P75X-ray3.02A/B/C/D23-352[»]
    ProteinModelPortaliP24425.
    SMRiP24425. Positions 23-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24425.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR001889. Herpes_TK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00693. Herpes_TK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P24425-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAACVPPGEA PRSASGTPTR RQVTIVRIYL DGVYGIGKST TGRVMASAAS    50
    GGSPTLYFPE PMAYWRTLFE TDVISGIYDT QNRKQQGNLA VDDAALITAH 100
    YQSRFTTPYL ILHDHTCTLF GGNSLQRGTQ PDLTLVFDRH PVASTVCFPA 150
    ARYLLGDMSM CALMAMVATL PREPQGGNIV VTTLNVEEHI RRLRTRARIG 200
    EQIDITLIAT LRNVYFMLVN TCHFLRSGRV WRDGWGELPT SCGAYKHRAT 250
    QMDAFQERVS PELGDTLFAL FKTQELLDDR GVILEVHAWA LDALMLKLRN 300
    LNVFSADLSG TPRQCAAVVE SLLPLMSSTL SDFDSASALE RAARTFNAEM 350
    GV 352
    Length:352
    Mass (Da):38,784
    Last modified:March 1, 1992 - v1
    Checksum:iF925ACF592077456
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14486 Genomic DNA. Translation: BAA03378.1.
    PIRiA36657. KIBEE4.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14486 Genomic DNA. Translation: BAA03378.1 .
    PIRi A36657. KIBEE4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1P6X X-ray 2.00 A/B 23-352 [» ]
    1P72 X-ray 2.10 A/B 23-352 [» ]
    1P73 X-ray 2.70 A/B/C/D 23-352 [» ]
    1P75 X-ray 3.02 A/B/C/D 23-352 [» ]
    ProteinModelPortali P24425.
    SMRi P24425. Positions 23-352.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P24425.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR001889. Herpes_TK.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00693. Herpes_TK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the equine herpesvirus 4 thymidine kinase gene."
      Nicolson L., Cullinane A.A., Onions D.E.
      J. Gen. Virol. 71:1801-1805(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases."
      Gardberg A., Shuvalova L., Monnerjahn C., Konrad M., Lavie A.
      Structure 11:1265-1277(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 23-352 IN COMPLEXES WITH THYMIDINE; ADP AND BISUBSTRATE ANALOGS, ACTIVE SITE, CATALYTIC ACTIVITY, SUBUNIT.

    Entry informationi

    Entry nameiKITH_EHV4
    AccessioniPrimary (citable) accession number: P24425
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3