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P24425

- KITH_EHV4

UniProt

P24425 - KITH_EHV4

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Protein

Thymidine kinase

Gene

TK

Organism
Equine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601Proton acceptorSequence Analysis
Binding sitei78 – 781Substrate
Binding sitei102 – 1021Substrate
Binding sitei105 – 1051Substrate
Binding sitei148 – 1481Substrate
Binding sitei192 – 1921ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 398ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. TMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
OrganismiEquine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Taxonomic identifieri10333 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Thymidine kinasePRO_0000175067Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
352
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 319
Helixi38 – 469
Helixi48 – 503
Beta strandi55 – 584
Helixi62 – 665
Beta strandi68 – 714
Helixi73 – 8614
Helixi91 – 11727
Beta strandi122 – 1254
Beta strandi132 – 1398
Helixi142 – 1454
Helixi147 – 1548
Helixi160 – 1678
Beta strandi177 – 1837
Helixi186 – 19611
Helixi205 – 22622
Turni231 – 2377
Helixi243 – 2497
Helixi263 – 2653
Helixi267 – 2715
Helixi274 – 2763
Helixi285 – 29814
Beta strandi301 – 3077
Helixi312 – 3209
Helixi321 – 3255
Beta strandi328 – 3325
Helixi333 – 35018

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P6XX-ray2.00A/B23-352[»]
1P72X-ray2.10A/B23-352[»]
1P73X-ray2.70A/B/C/D23-352[»]
1P75X-ray3.02A/B/C/D23-352[»]
ProteinModelPortaliP24425.
SMRiP24425. Positions 23-352.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24425.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P24425-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAACVPPGEA PRSASGTPTR RQVTIVRIYL DGVYGIGKST TGRVMASAAS
60 70 80 90 100
GGSPTLYFPE PMAYWRTLFE TDVISGIYDT QNRKQQGNLA VDDAALITAH
110 120 130 140 150
YQSRFTTPYL ILHDHTCTLF GGNSLQRGTQ PDLTLVFDRH PVASTVCFPA
160 170 180 190 200
ARYLLGDMSM CALMAMVATL PREPQGGNIV VTTLNVEEHI RRLRTRARIG
210 220 230 240 250
EQIDITLIAT LRNVYFMLVN TCHFLRSGRV WRDGWGELPT SCGAYKHRAT
260 270 280 290 300
QMDAFQERVS PELGDTLFAL FKTQELLDDR GVILEVHAWA LDALMLKLRN
310 320 330 340 350
LNVFSADLSG TPRQCAAVVE SLLPLMSSTL SDFDSASALE RAARTFNAEM

GV
Length:352
Mass (Da):38,784
Last modified:March 1, 1992 - v1
Checksum:iF925ACF592077456
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14486 Genomic DNA. Translation: BAA03378.1.
PIRiA36657. KIBEE4.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14486 Genomic DNA. Translation: BAA03378.1 .
PIRi A36657. KIBEE4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1P6X X-ray 2.00 A/B 23-352 [» ]
1P72 X-ray 2.10 A/B 23-352 [» ]
1P73 X-ray 2.70 A/B/C/D 23-352 [» ]
1P75 X-ray 3.02 A/B/C/D 23-352 [» ]
ProteinModelPortali P24425.
SMRi P24425. Positions 23-352.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P24425.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00693. Herpes_TK. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The nucleotide sequence of the equine herpesvirus 4 thymidine kinase gene."
    Nicolson L., Cullinane A.A., Onions D.E.
    J. Gen. Virol. 71:1801-1805(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases."
    Gardberg A., Shuvalova L., Monnerjahn C., Konrad M., Lavie A.
    Structure 11:1265-1277(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 23-352 IN COMPLEXES WITH THYMIDINE; ADP AND BISUBSTRATE ANALOGS, ACTIVE SITE, CATALYTIC ACTIVITY, SUBUNIT.

Entry informationi

Entry nameiKITH_EHV4
AccessioniPrimary (citable) accession number: P24425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 1, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3