P24425 (KITH_EHV4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:

TK

Organism

Equine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)

Taxonomic identifier

10333 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus › Equid herpesvirus 4

Virus host

Equus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome By similarity.
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. Ref.2
Subunit structure	Homodimer. Ref.2
Sequence similarities	Belongs to the herpesviruses thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW TMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 352

352

Thymidine kinase

PRO_0000175067

Regions

Nucleotide binding

32 – 39

8

ATP

Sites

Active site

60

1

Proton acceptor Potential

Binding site

78

1

Substrate

Binding site

102

1

Substrate

Binding site

105

1

Substrate

Binding site

148

1

Substrate

Binding site

192

1

ATP

Secondary structure

1

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352

Helix Strand Turn

Details...

Beta strand

23 – 31

9

Helix

38 – 46

9

Helix

48 – 50

3

Beta strand

55 – 58

4

Helix

62 – 66

5

Beta strand

68 – 71

4

Helix

73 – 86

14

Helix

91 – 117

27

Beta strand

122 – 125

4

Beta strand

132 – 139

8

Helix

142 – 145

4

Helix

147 – 154

8

Helix

160 – 167

8

Beta strand

177 – 183

7

Helix

186 – 196

11

Helix

205 – 226

22

Turn

231 – 237

7

Helix

243 – 249

7

Helix

263 – 265

3

Helix

267 – 271

5

Helix

274 – 276

3

Helix

285 – 298

14

Beta strand

301 – 307

7

Helix

312 – 320

9

Helix

321 – 325

5

Beta strand

328 – 332

5

Helix

333 – 350

18

Sequences

	Sequence		Length	Mass (Da)
	P24425 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: F925ACF592077456	FASTA	352	38,784
	10 20 30 40 50 60 MAACVPPGEA PRSASGTPTR RQVTIVRIYL DGVYGIGKST TGRVMASAAS GGSPTLYFPE 70 80 90 100 110 120 PMAYWRTLFE TDVISGIYDT QNRKQQGNLA VDDAALITAH YQSRFTTPYL ILHDHTCTLF 130 140 150 160 170 180 GGNSLQRGTQ PDLTLVFDRH PVASTVCFPA ARYLLGDMSM CALMAMVATL PREPQGGNIV 190 200 210 220 230 240 VTTLNVEEHI RRLRTRARIG EQIDITLIAT LRNVYFMLVN TCHFLRSGRV WRDGWGELPT 250 260 270 280 290 300 SCGAYKHRAT QMDAFQERVS PELGDTLFAL FKTQELLDDR GVILEVHAWA LDALMLKLRN 310 320 330 340 350 LNVFSADLSG TPRQCAAVVE SLLPLMSSTL SDFDSASALE RAARTFNAEM GV

References

[1]	"The nucleotide sequence of the equine herpesvirus 4 thymidine kinase gene." Nicolson L., Cullinane A.A., Onions D.E. J. Gen. Virol. 71:1801-1805(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases." Gardberg A., Shuvalova L., Monnerjahn C., Konrad M., Lavie A. Structure 11:1265-1277(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 23-352 IN COMPLEXES WITH THYMIDINE; ADP AND BISUBSTRATE ANALOGS, ACTIVE SITE, CATALYTIC ACTIVITY, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D14486 Genomic DNA. Translation: BAA03378.1.

PIR

KIBEE4. A36657.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P6X	X-ray	2.00	A/B	23-352	[»]
1P72	X-ray	2.10	A/B	23-352	[»]
1P73	X-ray	2.70	A/B/C/D	23-352	[»]
1P75	X-ray	3.02	A/B/C/D	23-352	[»]

ProteinModelPortal

P24425.

SMR

P24425. Positions 23-352.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]

Pfam

PF00693. Herpes_TK. 1 hit.
[Graphical view]

SUPFAM

SSF52540. SSF52540. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P24425.

Entry information

Entry name

KITH_EHV4

Accession

Primary (citable) accession number: P24425

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program