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Protein

Thymidine kinase

Gene

TK

Organism
Equine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60Proton acceptorUniRule annotation1
Binding sitei78SubstrateUniRule annotationCombined sources1 Publication1
Binding sitei102SubstrateUniRule annotationCombined sources1 Publication1
Binding sitei105SubstrateCombined sources1 Publication1
Binding sitei148SubstrateCombined sources1 Publication1
Binding sitei192ATPUniRule annotationCombined sources1 Publication1
Binding sitei198SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 39ATPUniRule annotationCombined sources1 Publication8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
OrganismiEquine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Taxonomic identifieri10333 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001750671 – 352Thymidine kinaseAdd BLAST352

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 31Combined sources9
Helixi38 – 46Combined sources9
Helixi48 – 50Combined sources3
Beta strandi55 – 58Combined sources4
Helixi62 – 66Combined sources5
Beta strandi68 – 71Combined sources4
Helixi73 – 86Combined sources14
Helixi91 – 117Combined sources27
Beta strandi122 – 125Combined sources4
Beta strandi132 – 139Combined sources8
Helixi142 – 145Combined sources4
Helixi147 – 154Combined sources8
Helixi160 – 167Combined sources8
Beta strandi177 – 183Combined sources7
Helixi186 – 196Combined sources11
Helixi205 – 226Combined sources22
Turni231 – 237Combined sources7
Helixi243 – 249Combined sources7
Helixi263 – 265Combined sources3
Helixi267 – 271Combined sources5
Helixi274 – 276Combined sources3
Helixi285 – 298Combined sources14
Beta strandi301 – 307Combined sources7
Helixi312 – 320Combined sources9
Helixi321 – 325Combined sources5
Beta strandi328 – 332Combined sources5
Helixi333 – 350Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P6XX-ray2.00A/B23-352[»]
1P72X-ray2.10A/B23-352[»]
1P73X-ray2.70A/B/C/D23-352[»]
1P75X-ray3.02A/B/C/D23-352[»]
ProteinModelPortaliP24425.
SMRiP24425.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24425.

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P24425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAACVPPGEA PRSASGTPTR RQVTIVRIYL DGVYGIGKST TGRVMASAAS
60 70 80 90 100
GGSPTLYFPE PMAYWRTLFE TDVISGIYDT QNRKQQGNLA VDDAALITAH
110 120 130 140 150
YQSRFTTPYL ILHDHTCTLF GGNSLQRGTQ PDLTLVFDRH PVASTVCFPA
160 170 180 190 200
ARYLLGDMSM CALMAMVATL PREPQGGNIV VTTLNVEEHI RRLRTRARIG
210 220 230 240 250
EQIDITLIAT LRNVYFMLVN TCHFLRSGRV WRDGWGELPT SCGAYKHRAT
260 270 280 290 300
QMDAFQERVS PELGDTLFAL FKTQELLDDR GVILEVHAWA LDALMLKLRN
310 320 330 340 350
LNVFSADLSG TPRQCAAVVE SLLPLMSSTL SDFDSASALE RAARTFNAEM

GV
Length:352
Mass (Da):38,784
Last modified:March 1, 1992 - v1
Checksum:iF925ACF592077456
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14486 Genomic DNA. Translation: BAA03378.1.
PIRiA36657. KIBEE4.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14486 Genomic DNA. Translation: BAA03378.1.
PIRiA36657. KIBEE4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P6XX-ray2.00A/B23-352[»]
1P72X-ray2.10A/B23-352[»]
1P73X-ray2.70A/B/C/D23-352[»]
1P75X-ray3.02A/B/C/D23-352[»]
ProteinModelPortaliP24425.
SMRiP24425.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP24425.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_EHV4
AccessioniPrimary (citable) accession number: P24425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.