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Protein

Ras-related protein Rab-9A

Gene

RAB9A

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of proteins between the endosomes and the trans-Golgi network (PubMed:8440258). Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155GTP; via amide nitrogenCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22GTPCombined sources1 Publication9
Nucleotide bindingi62 – 66GTPBy similarity5
Nucleotide bindingi124 – 127GTPCombined sources1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CFA-6811440. Retrograde transport at the Trans-Golgi-Network.
R-CFA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-9A
Gene namesi
Name:RAB9A
Synonyms:RAB9
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome X

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor Curated; Cytoplasmic side Curated
  • Endoplasmic reticulum membrane By similarity
  • Golgi apparatus membrane By similarity
  • Late endosome By similarity
  • Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor Curated; Cytoplasmic side Curated
  • Cytoplasmic vesiclephagosome By similarity
  • Cytoplasmic vesicle membrane By similarity
  • Melanosome By similarity

  • Note: Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001211382 – 201Ras-related protein Rab-9AAdd BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei179PhosphoserineBy similarity1
Modified residuei187PhosphothreonineBy similarity1
Lipidationi200S-geranylgeranyl cysteineBy similarity1
Lipidationi201S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP24408.
PRIDEiP24408.

Interactioni

Subunit structurei

Interacts (preferentially in its GTP-bound form) with GCC2 (via its GRIP domain) (PubMed:16885419). Interacts (GTP-bound form) with SGSM1; the GDP-bound form has much lower affinity for SGSM1. Interacts with SGSM2. The GTP-bound form but not the GDP-bound form interacts with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) but does not interact with HPS1 alone (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RHOBTB3O949555EBI-1646050,EBI-2367123From a different organism.

Protein-protein interaction databases

IntActiP24408. 2 interactors.
STRINGi9615.ENSCAFP00000017285.

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 13Combined sources7
Helixi20 – 29Combined sources10
Beta strandi38 – 51Combined sources14
Beta strandi54 – 63Combined sources10
Helixi67 – 69Combined sources3
Helixi70 – 73Combined sources4
Helixi74 – 76Combined sources3
Beta strandi81 – 88Combined sources8
Helixi92 – 96Combined sources5
Helixi98 – 109Combined sources12
Beta strandi119 – 124Combined sources6
Helixi135 – 144Combined sources10
Beta strandi150 – 152Combined sources3
Turni155 – 157Combined sources3
Helixi161 – 173Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S8FX-ray1.77A/B1-174[»]
ProteinModelPortaliP24408.
SMRiP24408.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24408.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi36 – 44Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP24408.
KOiK07899.
OMAiKIDINER.
OrthoDBiEOG091G0JIV.
TreeFamiTF326442.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKSSLFKV ILLGDGGVGK SSLMNRYVTN KFDTQLFHTI GVEFLNKDLE
60 70 80 90 100
VDGHFVTMQI WDTAGQERFR SLRTPFYRGS DCCLLTFSVD DSQSFQNLSN
110 120 130 140 150
WKKEFIYYAD VKEPESFPFV ILGNKIDISE RQVSTEEAQA WCRDNGDYPY
160 170 180 190 200
FETSAKDATN VAAAFEEAVR RVLATEDRSD HLIQTDTVSL HRKPKPSSSC

C
Length:201
Mass (Da):22,811
Last modified:October 1, 1994 - v2
Checksum:i65B502C204BEDB72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56386 mRNA. Translation: CAA39797.1.
PIRiS36187.
RefSeqiNP_001183960.1. NM_001197031.1.
XP_005641047.1. XM_005640990.2.
XP_013966791.1. XM_014111316.1.
UniGeneiCfa.316.

Genome annotation databases

EnsembliENSCAFT00000018660; ENSCAFP00000017285; ENSCAFG00000011750.
GeneIDi403947.
KEGGicfa:403947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56386 mRNA. Translation: CAA39797.1.
PIRiS36187.
RefSeqiNP_001183960.1. NM_001197031.1.
XP_005641047.1. XM_005640990.2.
XP_013966791.1. XM_014111316.1.
UniGeneiCfa.316.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S8FX-ray1.77A/B1-174[»]
ProteinModelPortaliP24408.
SMRiP24408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24408. 2 interactors.
STRINGi9615.ENSCAFP00000017285.

Proteomic databases

PaxDbiP24408.
PRIDEiP24408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000018660; ENSCAFP00000017285; ENSCAFG00000011750.
GeneIDi403947.
KEGGicfa:403947.

Organism-specific databases

CTDi9367.

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP24408.
KOiK07899.
OMAiKIDINER.
OrthoDBiEOG091G0JIV.
TreeFamiTF326442.

Enzyme and pathway databases

ReactomeiR-CFA-6811440. Retrograde transport at the Trans-Golgi-Network.
R-CFA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

EvolutionaryTraceiP24408.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB9A_CANLF
AccessioniPrimary (citable) accession number: P24408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.