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Protein

Ras-related protein Rab-9A

Gene

RAB9A

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of proteins between the endosomes and the trans-Golgi network.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 229GTP
Nucleotide bindingi62 – 665GTPBy similarity
Nucleotide bindingi124 – 1274GTP
Nucleotide bindingi154 – 1563GTP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CFA-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-9A
Gene namesi
Name:RAB9A
Synonyms:RAB9
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome X

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor Curated; Cytoplasmic side Curated
  • Endoplasmic reticulum membrane By similarity
  • Golgi apparatus membrane By similarity
  • Late endosome By similarity
  • Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor Curated; Cytoplasmic side Curated
  • Cytoplasmic vesiclephagosome By similarity

  • Note: Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 201200Ras-related protein Rab-9APRO_0000121138Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei179 – 1791PhosphoserineBy similarity
Modified residuei187 – 1871PhosphothreonineBy similarity
Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity
Lipidationi201 – 2011S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP24408.
PRIDEiP24408.

Interactioni

Subunit structurei

Interacts (preferentially in its GTP-bound form) with GCC2 (via its GRIP domain).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RHOBTB3O949555EBI-1646050,EBI-2367123From a different organism.

Protein-protein interaction databases

IntActiP24408. 2 interactions.
STRINGi9615.ENSCAFP00000017285.

Structurei

Secondary structure

1
201
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 137Combined sources
Helixi20 – 2910Combined sources
Beta strandi38 – 5114Combined sources
Beta strandi54 – 6310Combined sources
Helixi67 – 693Combined sources
Helixi70 – 734Combined sources
Helixi74 – 763Combined sources
Beta strandi81 – 888Combined sources
Helixi92 – 965Combined sources
Helixi98 – 10912Combined sources
Beta strandi119 – 1246Combined sources
Helixi135 – 14410Combined sources
Beta strandi150 – 1523Combined sources
Turni155 – 1573Combined sources
Helixi161 – 17313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S8FX-ray1.77A/B1-174[»]
ProteinModelPortaliP24408.
SMRiP24408. Positions 1-173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24408.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 449Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP24408.
KOiK07899.
OMAiKIDINER.
OrthoDBiEOG7G4QG8.
TreeFamiTF326442.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKSSLFKV ILLGDGGVGK SSLMNRYVTN KFDTQLFHTI GVEFLNKDLE
60 70 80 90 100
VDGHFVTMQI WDTAGQERFR SLRTPFYRGS DCCLLTFSVD DSQSFQNLSN
110 120 130 140 150
WKKEFIYYAD VKEPESFPFV ILGNKIDISE RQVSTEEAQA WCRDNGDYPY
160 170 180 190 200
FETSAKDATN VAAAFEEAVR RVLATEDRSD HLIQTDTVSL HRKPKPSSSC

C
Length:201
Mass (Da):22,811
Last modified:October 1, 1994 - v2
Checksum:i65B502C204BEDB72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56386 mRNA. Translation: CAA39797.1.
PIRiS36187.
RefSeqiNP_001183960.1. NM_001197031.1.
XP_005641047.1. XM_005640990.2.
XP_013966791.1. XM_014111316.1.
UniGeneiCfa.316.

Genome annotation databases

EnsembliENSCAFT00000018660; ENSCAFP00000017285; ENSCAFG00000011750.
GeneIDi403947.
KEGGicfa:403947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56386 mRNA. Translation: CAA39797.1.
PIRiS36187.
RefSeqiNP_001183960.1. NM_001197031.1.
XP_005641047.1. XM_005640990.2.
XP_013966791.1. XM_014111316.1.
UniGeneiCfa.316.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S8FX-ray1.77A/B1-174[»]
ProteinModelPortaliP24408.
SMRiP24408. Positions 1-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24408. 2 interactions.
STRINGi9615.ENSCAFP00000017285.

Proteomic databases

PaxDbiP24408.
PRIDEiP24408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000018660; ENSCAFP00000017285; ENSCAFG00000011750.
GeneIDi403947.
KEGGicfa:403947.

Organism-specific databases

CTDi9367.

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP24408.
KOiK07899.
OMAiKIDINER.
OrthoDBiEOG7G4QG8.
TreeFamiTF326442.

Enzyme and pathway databases

ReactomeiR-CFA-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

EvolutionaryTraceiP24408.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rab9 functions in transport between late endosomes and the trans Golgi network."
    Lombardi D., Soldati T., Riederer M.A., Goda Y., Zerial M., Pfeffer S.R.
    EMBO J. 12:677-682(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Cocker spaniel.
    Tissue: Kidney.
  2. "Molecular cloning of YPT1/SEC4-related cDNAs from an epithelial cell line."
    Chavrier P., Vingron M., Sander C., Simons K., Zerial M.
    Mol. Cell. Biol. 10:6578-6585(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-201.
    Strain: Cocker spaniel.
  3. "A functional role for the GCC185 golgin in mannose 6-phosphate receptor recycling."
    Reddy J.V., Burguete A.S., Sridevi K., Ganley I.G., Nottingham R.M., Pfeffer S.R.
    Mol. Biol. Cell 17:4353-4363(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GCC2.
  4. "Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II."
    Wittmann J.G., Rudolph M.G.
    FEBS Lett. 568:23-29(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 1-174 IN COMPLEX WITH GDP.

Entry informationi

Entry nameiRAB9A_CANLF
AccessioniPrimary (citable) accession number: P24408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1994
Last modified: June 8, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.