Reviewed, UniProtKB/Swiss-Prot P24400 (PTLCB_LACCA)

Names and origin

Protein names

Recommended name:
    PTS system lactose-specific EIICB component
Alternative name(s):
    EIICB-Lac
      Short name=EII-Lac
Including the following 2 domains:
    1- Recommended name:
            Lactose permease IIC component
        Alternative name(s):
            PTS system lactose-specific EIIC component
    2- Recommended name:
            Lactose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system lactose-specific EIIB component

Gene names

Name:

lacE

Organism

Lactobacillus casei

Taxonomic identifier

1582 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-3 domain. Contains 1 PTS EIIC type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	PTS system lactose-specific EIICB component		PRO_0000186583
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	63 – 83	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	176 – 196	21	Potential
	Transmembrane	219 – 239	21	Potential
	Transmembrane	280 – 300	21	Potential
	Transmembrane	326 – 346	21	Potential
	Transmembrane	386 – 406	21	Potential
	Domain	4 – 405	402	PTS EIIC type-3
	Domain	476 – 577	102	PTS EIIB type-3
Sites
	Active site	483	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24400-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: BE56852CAB0D76E4	FASTA	577	62,392
	10 20 30 40 50 60 MNKVFDKLKP VFEAIAANKY ISAIRDGFIA CMPIIIFSSI FMMVAYVPNA WGFYWPDNVT 70 80 90 100 110 120 NTLMVAYNYS MGLLALFVAG TTAKNLTDSK NLELPKTNQI NPVAVIVASE ISFVILSILP 130 140 150 160 170 180 LKTGVDLTYM GTQGLICAYI VGLIVPNIYY VCIKNNVTIK LPEQVPGNIA QSFKDLIPMG 190 200 210 220 230 240 LSVTAFWLFG VGFKAATGTV LPRWIIQVLS PLFQASDSYL GLALIAGAMA FFWFCGVQGP 250 260 270 280 290 300 SIVQPAVVPI MIANTAANLQ QYQAGQHVSH VLAMNTMDYV MNFGGTGATL VVPFIMLFAA 310 320 330 340 350 360 RSAQLKAVGK AAFVPCTFGV NEPVLFGMPI IMNPMLFIPF LATPIVNVCL FKFFVSVLGM 370 380 390 400 410 420 NSMMYTMPWT VPGPIGILIS TGFAPLAFVF VLLTLVLDVA IYFPFIRVYD STLLAEEKAK 430 440 450 460 470 480 EEVIEDDGMA VLASDTVSPS IPTGLTVATA TDDDATHVLP ETAPSAHGEA YFKQNEVDVL 490 500 510 520 530 540 VLCAGGGTSG ILANALNKLS KERGLKLSAA ARAYGQDMDL IKDMNMVILA PQMESMKGNL 550 560 570 KKITDKYGVK LVTTTGRQYI ELTNNGDMAL DFVESNL

References

[1]

"Molecular cloning and DNA sequence of lacE, the gene encoding the lactose-specific enzyme II of the phosphotransferase system of Lactobacillus casei. Evidence that a cysteine residue is essential for sugar phosphorylation." Alpert C.-A., Chassy B.M. J. Biol. Chem. 265:22561-22568(1990) [PubMed: 2125053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60851 Genomic DNA. Translation: AAA72984.1.
PIR	B23697.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.69. 610.
Family and domain databases
InterPro	IPR014350. PTrfase_system_EIIB_3_subgr. IPR013012. PTS_EIIB_3. IPR003352. PTS_EIIC. IPR003501. PTS_IIB_lac. IPR004501. PTS_IIC_lac. IPR004801. PTS_IIC_lac_spec. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00394. lac_pts_IIC. 1 hit. TIGR00410. lacE. 1 hit. TIGR00853. pts-lac. 1 hit.
PROSITE	PS51100. PTS_EIIB_TYPE_3. 1 hit. PS51105. PTS_EIIC_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTLCB_LACCA

Accession

Primary (citable) accession number: P24400

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)