Reviewed, UniProtKB/Swiss-Prot P24397 (HY6H_HYONI)

Names and origin

Protein names

Recommended name:
    Hyoscyamine 6-dioxygenase
    EC=1.14.11.11
Alternative name(s):
    Hyoscyamine 6-beta-hydroxylase

Gene names

Name:

H6H

Organism

Hyoscyamus niger (Black henbane)

Taxonomic identifier

4079 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Hyoscyameae › Hyoscyamus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-hyoscyamine + 2-oxoglutarate + O2 = (6S)-hydroxyhyoscyamine + succinate + CO2.
Cofactor	Iron. Ascorbate.
Pathway	Alkaloid biosynthesis; scopolamine biosynthesis.
Subunit structure	Monomer.
Tissue specificity	Root.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW hyoscyamine (6S)-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Hyoscyamine 6-dioxygenase		PRO_0000067281
Regions
	Domain	193 – 293	101	Fe2OG dioxygenase
Sites
	Metal binding	217	1	Iron Potential
	Metal binding	274	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	P24397-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 2618501AC1587F24	FASTA	344	39,001
	10 20 30 40 50 60 MATFVSNWST KSVSESFIAP LQKRAEKDVP VGNDVPIIDL QQHHHLLVQQ ITKACQDFGL 70 80 90 100 110 120 FQVINHGFPE ELMLETMEVC KEFFALPAEE KEKFKPKGEA AKFELPLEQK AKLYVEGEQL 130 140 150 160 170 180 SNEEFLYWKD TLAHGCHPLD QDLVNSWPEK PAKYREVVAK YSVEVRKLTM RMLDYICEGL 190 200 210 220 230 240 GLKLGYFDNE LSQIQMMLTN YYPPCPDPSS TLGSGGHYDG NLITLLQQDL PGLQQLIVKD 250 260 270 280 290 300 ATWIAVQPIP TAFVVNLGLT LKVITNEKFE GSIHRVVTDP TRDRVSIATL IGPDYSCTIE 310 320 330 340 PAKELLNQDN PPLYKPYSYS EFADIYLSDK SDYDSGVKPY KINV

References

[1]	"Molecular cloning of hyoscyamine 6 beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger." Matsuda J., Okabe S., Hashimoto T., Yamada Y. J. Biol. Chem. 266:9460-9464(1991) [PubMed: 2033047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE. Tissue: Root.
[2]	"Species-dependent expression of the hyoscyamine 6 beta-hydroxylase gene in the pericycle." Kanegae T., Kajiya H., Amano Y., Hashimoto T., Yamada Y. Plant Physiol. 105:483-490(1994) [PubMed: 8066129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62719 mRNA. Translation: AAA33387.1. D26583 Genomic DNA. Translation: BAA05630.1.
PIR	A40005.
3D structure databases
SMR	P24397. Positions 9-331.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.11. 95966.
Family and domain databases
InterPro	IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HY6H_HYONI

Accession

Primary (citable) accession number: P24397

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	January 19, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)