P24396 (PLY18_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase P18
EC=4.2.2.2

Alternative name(s):
Style development-specific protein 9612

Gene names

Name:

9612

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in the development of the transmitting tissue of the style and/or in the events related to pollination such as some aspect in the facilitation of compatible pollen tube growth.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Tissue specificity	Predominantly found in the pistil where it is found in the outer five layers of the strands of transmitting tissue within the upper two-thirds of the style. Found at much lower levels in the anthers and vegetative organs.
Developmental stage	Maximum levels are found during anthesis.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 404	384	Probable pectate lyase P18		PRO_0000024889
Sites
	Active site	280	1	Potential
	Metal binding	200	1	Calcium By similarity
	Metal binding	224	1	Calcium By similarity
	Metal binding	228	1	Calcium By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P24396 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: B26ED69B128D8675	FASTA	404	44,298
	10 20 30 40 50 60 MSTLFFTFSL LLLAPLLVIS SIQDPELVVQ DVHRSINASL TRRNLGYLSC GSGNPIDRLL 70 80 90 100 110 120 AMQPQLGKKS PAFSYCAIGF GKNAIGGKNG RIYVVTDSGN DDPVNPKPGT LRHAVIQDEP 130 140 150 160 170 180 LWIIFKRDMV IQLKQELVMN SYKTIDGRGA SVHISGGPCI TIHHTSNIII HGINIHDCKQ 190 200 210 220 230 240 SGNGNIRDSP NHSGWWDVSD GDGISIFGGK NIWVDHCSLS NCHDGLIDAI HGSTAITISN 250 260 270 280 290 300 NYFTHHDKVM LLGHSDSFTQ DKGMQVTVAF NHFGEGLVQR MPRCRHGYFH VVNNDYTHWE 310 320 330 340 350 360 MYAIGGSAAP TINSQGNRFL APNEKYRKEV TKHEDAPESQ WRSWNWRSEG DLMLNGAYFR 370 380 390 400 QTGAGASSSS TYARASSLSA RPSSLVGSIT TNAGPVNCKK GSRC

References

[1]	"Regulation of a stylar transmitting tissue-specific gene in wild-type and transgenic tomato and tobacco." Budelier K.A., Smith A.G., Gasser C.S. Mol. Gen. Genet. 224:183-192(1990) [PubMed: 2277637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. VF36. Tissue: Pistil.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55193 mRNA. Translation: CAA38979.1.
PIR	S12209.
RefSeq	NP_001234029.1. NM_001247100.1.
UniGene	Les.3663.
3D structure databases
ProteinModelPortal	P24396.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	778293.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY18_SOLLC

Accession

Primary (citable) accession number: P24396

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program