P24389 (IOD1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type I iodothyronine deiodinase
EC=1.97.1.10

Alternative name(s):
5DI
DIOI
Type 1 DI
Type-I 5'-deiodinase

Gene names

Name:	Dio1
Synonyms:	Itdi1, Txdi1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).
Catalytic activity	3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein.
Tissue specificity	Highly expressed in the liver, kidney and thyroid gland of adult rats.
Sequence similarities	Belongs to the iodothyronine deiodinase family.

Ontologies

Keywords
Biological process	Thyroid hormones biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Selenocysteine
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	generation of precursor metabolites and energy Non-traceable author statement PubMed 11765219. Source: RGD hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	thyroxine 5'-deiodinase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Type I iodothyronine deiodinase		PRO_0000154313
Regions
	Transmembrane	13 – 33	21	Helical; Potential
Sites
	Active site	126	1
Amino acid modifications
	Non-standard residue	126	1	Selenocysteine
Experimental info
	Mutagenesis	158	1	H → F, Q or N: Loss of enzyme activity. Ref.3
	Mutagenesis	174	1	H → N: 100-fold increase in KM. Ref.3
	Mutagenesis	174	1	H → Q: 20-fold increase in KM. Ref.3
	Mutagenesis	185	1	H → N: No effect on enzyme activity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P24389 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 3142ADB974A3E184	FASTA	257	29,730
	10 20 30 40 50 60 MGLSQLWLWL KRLVIFLQVA LEVATGKVLM TLFPERVKQN ILAMGQKTGM TRNPRFAPDN 70 80 90 100 110 120 WVPTFFSIQY FWFVLKVRWQ RLEDRAEYGG LAPNCTVVRL SGQKCNVWDF IQGSRPLVLN 130 140 150 160 170 180 FGSCTUPSFL LKFDQFKRLV DDFASTADFL IIYIEEAHAT DGWAFKNNVD IRQHRSLQDR 190 200 210 220 230 240 LRAAHLLLAR SPQCPVVVDT MQNQSSQLYA ALPERLYVIQ EGRICYKGKP GPWNYNPEEV 250 RAVLEKLCIP PGHMPQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Type I iodothyronine deiodinase is a selenocysteine-containing enzyme." Berry M.J., Banu L., Larsen P.R. Nature 349:438-440(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Identification of essential histidine residues in rat type I iodothyronine deiodinase." Berry M.J. J. Biol. Chem. 267:18055-18059(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF HIS-158; HIS-174 AND HIS-185.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57999 mRNA. Translation: CAA41063.1. BC083557 mRNA. Translation: AAH83557.1.
IPI	IPI00213691.
PIR	S13642.
RefSeq	NP_067685.5. NM_021653.3.
UniGene	Rn.87549.
3D structure databases
ProteinModelPortal	P24389.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000040061.
Proteomic databases
PRIDE	P24389.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25430.
KEGG	rno:25430.
UCSC	RGD:2504. rat.
Organism-specific databases
CTD	1733.
RGD	2504. Dio1.
Phylogenomic databases
eggNOG	NOG149534.
HOVERGEN	HBG000099.
InParanoid	P24389.
KO	K01562.
OMA	NHQNLQD.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14889.
BRENDA	1.97.1.10. 5301.
SABIO-RK	P24389.
Gene expression databases
Genevestigator	P24389.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000643. Iodothyronine_deiodinase. IPR008261. Iodothyronine_deiodinase_AS. IPR027252. Iodothyronine_deiodinase_I/III. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11781. PTHR11781. 1 hit.
Pfam	PF00837. T4_deiodinase. 1 hit. [Graphical view]
PIRSF	PIRSF001330. IOD. 1 hit. PIRSF500144. IODI_III. 1 hit.
PROSITE	PS01205. T4_DEIODINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606621.

Entry information

Entry name

IOD1_RAT

Accession

Primary (citable) accession number: P24389

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program