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P24389

- IOD1_RAT

UniProt

P24389 - IOD1_RAT

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Protein

Type I iodothyronine deiodinase

Gene

Dio1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei126 – 1261

GO - Molecular functioni

  1. thyroxine 5'-deiodinase activity Source: RGD

GO - Biological processi

  1. generation of precursor metabolites and energy Source: RGD
  2. hormone biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Thyroid hormones biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14889.
BRENDAi1.97.1.10. 5301.
SABIO-RKP24389.

Names & Taxonomyi

Protein namesi
Recommended name:
Type I iodothyronine deiodinase (EC:1.97.1.10)
Alternative name(s):
5DI
DIOI
Type 1 DI
Type-I 5'-deiodinase
Gene namesi
Name:Dio1
Synonyms:Itdi1, Txdi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2504. Dio1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi158 – 1581H → F, Q or N: Loss of enzyme activity. 1 Publication
Mutagenesisi174 – 1741H → N: 100-fold increase in KM. 1 Publication
Mutagenesisi174 – 1741H → Q: 20-fold increase in KM. 1 Publication
Mutagenesisi185 – 1851H → N: No effect on enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Type I iodothyronine deiodinasePRO_0000154313Add
BLAST

Proteomic databases

PRIDEiP24389.

Expressioni

Tissue specificityi

Highly expressed in the liver, kidney and thyroid gland of adult rats.

Gene expression databases

GenevestigatoriP24389.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040061.

Structurei

3D structure databases

ProteinModelPortaliP24389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the iodothyronine deiodinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG149534.
HOVERGENiHBG000099.
InParanoidiP24389.
KOiK01562.
PhylomeDBiP24389.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR027252. Iodothyronine_deiodinase_I/III.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
PIRSF500144. IODI_III. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24389-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLSQLWLWL KRLVIFLQVA LEVATGKVLM TLFPERVKQN ILAMGQKTGM
60 70 80 90 100
TRNPRFAPDN WVPTFFSIQY FWFVLKVRWQ RLEDRAEYGG LAPNCTVVRL
110 120 130 140 150
SGQKCNVWDF IQGSRPLVLN FGSCTUPSFL LKFDQFKRLV DDFASTADFL
160 170 180 190 200
IIYIEEAHAT DGWAFKNNVD IRQHRSLQDR LRAAHLLLAR SPQCPVVVDT
210 220 230 240 250
MQNQSSQLYA ALPERLYVIQ EGRICYKGKP GPWNYNPEEV RAVLEKLCIP

PGHMPQF
Length:257
Mass (Da):29,730
Last modified:February 26, 2008 - v2
Checksum:i3142ADB974A3E184
GO

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei126 – 1261Selenocysteine

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57999 mRNA. Translation: CAA41063.1.
BC083557 mRNA. Translation: AAH83557.1.
PIRiS13642.
RefSeqiNP_067685.5. NM_021653.3.
UniGeneiRn.87549.

Genome annotation databases

GeneIDi25430.
KEGGirno:25430.
UCSCiRGD:2504. rat.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57999 mRNA. Translation: CAA41063.1 .
BC083557 mRNA. Translation: AAH83557.1 .
PIRi S13642.
RefSeqi NP_067685.5. NM_021653.3.
UniGenei Rn.87549.

3D structure databases

ProteinModelPortali P24389.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000040061.

Proteomic databases

PRIDEi P24389.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25430.
KEGGi rno:25430.
UCSCi RGD:2504. rat.

Organism-specific databases

CTDi 1733.
RGDi 2504. Dio1.

Phylogenomic databases

eggNOGi NOG149534.
HOVERGENi HBG000099.
InParanoidi P24389.
KOi K01562.
PhylomeDBi P24389.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-14889.
BRENDAi 1.97.1.10. 5301.
SABIO-RK P24389.

Miscellaneous databases

NextBioi 606621.
PROi P24389.

Gene expression databases

Genevestigatori P24389.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR027252. Iodothyronine_deiodinase_I/III.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
PANTHERi PTHR11781. PTHR11781. 1 hit.
Pfami PF00837. T4_deiodinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001330. IOD. 1 hit.
PIRSF500144. IODI_III. 1 hit.
SUPFAMi SSF52833. SSF52833. 1 hit.
PROSITEi PS01205. T4_DEIODINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Type I iodothyronine deiodinase is a selenocysteine-containing enzyme."
    Berry M.J., Banu L., Larsen P.R.
    Nature 349:438-440(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Identification of essential histidine residues in rat type I iodothyronine deiodinase."
    Berry M.J.
    J. Biol. Chem. 267:18055-18059(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-158; HIS-174 AND HIS-185.

Entry informationi

Entry nameiIOD1_RAT
AccessioniPrimary (citable) accession number: P24389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 26, 2008
Last modified: October 29, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3