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P24389 (IOD1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type I iodothyronine deiodinase

EC=1.97.1.10
Alternative name(s):
5DI
DIOI
Type 1 DI
Type-I 5'-deiodinase
Gene names
Name:Dio1
Synonyms:Itdi1, Txdi1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).

Catalytic activity

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein.

Tissue specificity

Highly expressed in the liver, kidney and thyroid gland of adult rats.

Sequence similarities

Belongs to the iodothyronine deiodinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Type I iodothyronine deiodinase
PRO_0000154313

Regions

Transmembrane13 – 3321Helical; Potential

Sites

Active site1261

Amino acid modifications

Non-standard residue1261Selenocysteine

Experimental info

Mutagenesis1581H → F, Q or N: Loss of enzyme activity. Ref.3
Mutagenesis1741H → N: 100-fold increase in KM. Ref.3
Mutagenesis1741H → Q: 20-fold increase in KM. Ref.3
Mutagenesis1851H → N: No effect on enzyme activity. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P24389 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: 3142ADB974A3E184

FASTA25729,730
        10         20         30         40         50         60 
MGLSQLWLWL KRLVIFLQVA LEVATGKVLM TLFPERVKQN ILAMGQKTGM TRNPRFAPDN 

        70         80         90        100        110        120 
WVPTFFSIQY FWFVLKVRWQ RLEDRAEYGG LAPNCTVVRL SGQKCNVWDF IQGSRPLVLN 

       130        140        150        160        170        180 
FGSCTUPSFL LKFDQFKRLV DDFASTADFL IIYIEEAHAT DGWAFKNNVD IRQHRSLQDR 

       190        200        210        220        230        240 
LRAAHLLLAR SPQCPVVVDT MQNQSSQLYA ALPERLYVIQ EGRICYKGKP GPWNYNPEEV 

       250 
RAVLEKLCIP PGHMPQF 

« Hide

References

« Hide 'large scale' references
[1]"Type I iodothyronine deiodinase is a selenocysteine-containing enzyme."
Berry M.J., Banu L., Larsen P.R.
Nature 349:438-440(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]"Identification of essential histidine residues in rat type I iodothyronine deiodinase."
Berry M.J.
J. Biol. Chem. 267:18055-18059(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF HIS-158; HIS-174 AND HIS-185.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57999 mRNA. Translation: CAA41063.1.
BC083557 mRNA. Translation: AAH83557.1.
PIRS13642.
RefSeqNP_067685.5. NM_021653.3.
UniGeneRn.87549.

3D structure databases

ProteinModelPortalP24389.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000040061.

Proteomic databases

PRIDEP24389.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25430.
KEGGrno:25430.
UCSCRGD:2504. rat.

Organism-specific databases

CTD1733.
RGD2504. Dio1.

Phylogenomic databases

eggNOGNOG149534.
HOVERGENHBG000099.
InParanoidP24389.
KOK01562.
PhylomeDBP24389.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14889.
BRENDA1.97.1.10. 5301.
SABIO-RKP24389.

Gene expression databases

GenevestigatorP24389.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR027252. Iodothyronine_deiodinase_I/III.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERPTHR11781. PTHR11781. 1 hit.
PfamPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFPIRSF001330. IOD. 1 hit.
PIRSF500144. IODI_III. 1 hit.
SUPFAMSSF52833. SSF52833. 1 hit.
PROSITEPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio606621.
PROP24389.

Entry information

Entry nameIOD1_RAT
AccessionPrimary (citable) accession number: P24389
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 26, 2008
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families