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P24389

- IOD1_RAT

UniProt

P24389 - IOD1_RAT

Protein

Type I iodothyronine deiodinase

Gene

Dio1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).

    Catalytic activityi

    3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei126 – 1261

    GO - Molecular functioni

    1. thyroxine 5'-deiodinase activity Source: RGD

    GO - Biological processi

    1. generation of precursor metabolites and energy Source: RGD
    2. hormone biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Thyroid hormones biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14889.
    BRENDAi1.97.1.10. 5301.
    SABIO-RKP24389.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type I iodothyronine deiodinase (EC:1.97.1.10)
    Alternative name(s):
    5DI
    DIOI
    Type 1 DI
    Type-I 5'-deiodinase
    Gene namesi
    Name:Dio1
    Synonyms:Itdi1, Txdi1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2504. Dio1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi158 – 1581H → F, Q or N: Loss of enzyme activity. 1 Publication
    Mutagenesisi174 – 1741H → N: 100-fold increase in KM. 1 Publication
    Mutagenesisi174 – 1741H → Q: 20-fold increase in KM. 1 Publication
    Mutagenesisi185 – 1851H → N: No effect on enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 257257Type I iodothyronine deiodinasePRO_0000154313Add
    BLAST

    Proteomic databases

    PRIDEiP24389.

    Expressioni

    Tissue specificityi

    Highly expressed in the liver, kidney and thyroid gland of adult rats.

    Gene expression databases

    GenevestigatoriP24389.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000040061.

    Structurei

    3D structure databases

    ProteinModelPortaliP24389.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the iodothyronine deiodinase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG149534.
    HOVERGENiHBG000099.
    InParanoidiP24389.
    KOiK01562.
    PhylomeDBiP24389.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000643. Iodothyronine_deiodinase.
    IPR008261. Iodothyronine_deiodinase_AS.
    IPR027252. Iodothyronine_deiodinase_I/III.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR11781. PTHR11781. 1 hit.
    PfamiPF00837. T4_deiodinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001330. IOD. 1 hit.
    PIRSF500144. IODI_III. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS01205. T4_DEIODINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24389-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLSQLWLWL KRLVIFLQVA LEVATGKVLM TLFPERVKQN ILAMGQKTGM    50
    TRNPRFAPDN WVPTFFSIQY FWFVLKVRWQ RLEDRAEYGG LAPNCTVVRL 100
    SGQKCNVWDF IQGSRPLVLN FGSCTUPSFL LKFDQFKRLV DDFASTADFL 150
    IIYIEEAHAT DGWAFKNNVD IRQHRSLQDR LRAAHLLLAR SPQCPVVVDT 200
    MQNQSSQLYA ALPERLYVIQ EGRICYKGKP GPWNYNPEEV RAVLEKLCIP 250
    PGHMPQF 257
    Length:257
    Mass (Da):29,730
    Last modified:February 26, 2008 - v2
    Checksum:i3142ADB974A3E184
    GO

    Non-standard residue

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-standard residuei126 – 1261Selenocysteine

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57999 mRNA. Translation: CAA41063.1.
    BC083557 mRNA. Translation: AAH83557.1.
    PIRiS13642.
    RefSeqiNP_067685.5. NM_021653.3.
    UniGeneiRn.87549.

    Genome annotation databases

    GeneIDi25430.
    KEGGirno:25430.
    UCSCiRGD:2504. rat.

    Keywords - Coding sequence diversityi

    Selenocysteine

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57999 mRNA. Translation: CAA41063.1 .
    BC083557 mRNA. Translation: AAH83557.1 .
    PIRi S13642.
    RefSeqi NP_067685.5. NM_021653.3.
    UniGenei Rn.87549.

    3D structure databases

    ProteinModelPortali P24389.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000040061.

    Proteomic databases

    PRIDEi P24389.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25430.
    KEGGi rno:25430.
    UCSCi RGD:2504. rat.

    Organism-specific databases

    CTDi 1733.
    RGDi 2504. Dio1.

    Phylogenomic databases

    eggNOGi NOG149534.
    HOVERGENi HBG000099.
    InParanoidi P24389.
    KOi K01562.
    PhylomeDBi P24389.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14889.
    BRENDAi 1.97.1.10. 5301.
    SABIO-RK P24389.

    Miscellaneous databases

    NextBioi 606621.
    PROi P24389.

    Gene expression databases

    Genevestigatori P24389.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR000643. Iodothyronine_deiodinase.
    IPR008261. Iodothyronine_deiodinase_AS.
    IPR027252. Iodothyronine_deiodinase_I/III.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR11781. PTHR11781. 1 hit.
    Pfami PF00837. T4_deiodinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001330. IOD. 1 hit.
    PIRSF500144. IODI_III. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    PROSITEi PS01205. T4_DEIODINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Type I iodothyronine deiodinase is a selenocysteine-containing enzyme."
      Berry M.J., Banu L., Larsen P.R.
      Nature 349:438-440(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. "Identification of essential histidine residues in rat type I iodothyronine deiodinase."
      Berry M.J.
      J. Biol. Chem. 267:18055-18059(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF HIS-158; HIS-174 AND HIS-185.

    Entry informationi

    Entry nameiIOD1_RAT
    AccessioniPrimary (citable) accession number: P24389
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3