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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

Gene

PRP22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts late in the splicing of pre-mRNA. Mediates the release of the spliced mRNA from spliceosomes.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi506 – 513ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • poly(A) RNA binding Source: GO_Central

GO - Biological processi

  • generation of catalytic spliceosome for second transesterification step Source: SGD
  • spliceosomal complex disassembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30200-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 (EC:3.6.4.13)
Gene namesi
Name:PRP22
Ordered Locus Names:YER013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER013W.
SGDiS000000815. PRP22.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • U2-type catalytic step 2 spliceosome Source: SGD
  • U2-type post-spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551351 – 1145Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22Add BLAST1145

Proteomic databases

MaxQBiP24384.
PRIDEiP24384.

PTM databases

iPTMnetiP24384.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi36745. 182 interactors.
DIPiDIP-655N.
IntActiP24384. 3 interactors.
MINTiMINT-648209.

Structurei

3D structure databases

ProteinModelPortaliP24384.
SMRiP24384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 250S1 motifPROSITE-ProRule annotationAdd BLAST73
Domaini493 – 656Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST164
Domaini678 – 854Helicase C-terminalPROSITE-ProRule annotationAdd BLAST177

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi603 – 606DEAH box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
InParanoidiP24384.
KOiK12818.
OMAiRNPEWLV.
OrthoDBiEOG092C2SAZ.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE
60 70 80 90 100
DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDVN LDDPVVTEFV
110 120 130 140 150
LSILNKSKSI TEFQEQLNLM QSGLDNETIF KIYQIASPPV MKEEVSVLPS
160 170 180 190 200
TKIPAKIEAK IEEEVQKIES LDPSPVLHKV YEGKVRNITT FGCFVQIFGT
210 220 230 240 250
RMKNCDGLVH ISEMSDQRTL DPHDVVRQGQ HIFVEVIKIQ NNGKISLSMK
260 270 280 290 300
NIDQHSGEIR KRNTESVEDR GRSNDAHTSR NMKNKIKRRA LTSPERWEIR
310 320 330 340 350
QLIASGAASI DDYPELKDEI PINTSYLTAK RDDGSIVNGN TEKVDSKLEE
360 370 380 390 400
QQRDETDEID VELNTDDGPK FLKDQQVKGA KKYEMPKITK VPRGFMNRSA
410 420 430 440 450
INGSNAIRDH REEKLRKKRE IEQQIRKQQS FDDPTKNKKD SRNEIQMLKN
460 470 480 490 500
QLIVTEWEKN RMNESISYGK RTSLPISAQR QTLPVYAMRS ELIQAVRDNQ
510 520 530 540 550
FLVIVGETGS GKTTQITQYL DEEGFSNYGM IGCTQPRRVA AVSVAKRVAE
560 570 580 590 600
EVGCKVGHDV GYTIRFEDVT GPDTRIKYMT DGMLQREALL DPEMSKYSVI
610 620 630 640 650
MLDEAHERTV ATDVLFALLK KAAIKRPELK VIVTSATLNS AKFSEYFLNC
660 670 680 690 700
PIINIPGKTF PVEVLYSQTP QMDYIEAALD CVIDIHINEG PGDILVFLTG
710 720 730 740 750
QEEIDSCCEI LYDRVKTLGD SIGELLILPV YSALPSEIQS KIFEPTPKGS
760 770 780 790 800
RKVVFATNIA ETSITIDGIY YVVDPGFAKI NIYNARAGIE QLIVSPISQA
810 820 830 840 850
QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML
860 870 880 890 900
KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEGK LTNLGKEMSL
910 920 930 940 950
FPMDPTLSRS LLSSVDNQCS DEIVTIISML SVQNVFYRPK DRQLEADSKK
960 970 980 990 1000
AKFHHPYGDH LTLLNVYTRW QQANYSEQYC KTNFLHFRHL KRARDVKSQI
1010 1020 1030 1040 1050
SMIFKKIGLK LISCHSDPDL IRKTFVSGFF MNAAKRDSQV GYKTINGGTE
1060 1070 1080 1090 1100
VGIHPSSSLY GKEYEYVMYH SIVLTSREYM SQVTSIEPQW LLEVAPHFYK
1110 1120 1130 1140
AGDAESQSRK KAKIIPLHNK FAKDQNSWRL SSIRQSRERA LGIKR
Length:1,145
Mass (Da):130,011
Last modified:March 1, 1992 - v1
Checksum:iD92DBD620531B9A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58681 Genomic DNA. Translation: CAA41530.1.
U18778 Genomic DNA. Translation: AAB64546.1.
BK006939 Genomic DNA. Translation: DAA07664.1.
PIRiS13643.
RefSeqiNP_010929.3. NM_001178904.3.

Genome annotation databases

EnsemblFungiiYER013W; YER013W; YER013W.
GeneIDi856732.
KEGGisce:YER013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58681 Genomic DNA. Translation: CAA41530.1.
U18778 Genomic DNA. Translation: AAB64546.1.
BK006939 Genomic DNA. Translation: DAA07664.1.
PIRiS13643.
RefSeqiNP_010929.3. NM_001178904.3.

3D structure databases

ProteinModelPortaliP24384.
SMRiP24384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36745. 182 interactors.
DIPiDIP-655N.
IntActiP24384. 3 interactors.
MINTiMINT-648209.

PTM databases

iPTMnetiP24384.

Proteomic databases

MaxQBiP24384.
PRIDEiP24384.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER013W; YER013W; YER013W.
GeneIDi856732.
KEGGisce:YER013W.

Organism-specific databases

EuPathDBiFungiDB:YER013W.
SGDiS000000815. PRP22.

Phylogenomic databases

GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
InParanoidiP24384.
KOiK12818.
OMAiRNPEWLV.
OrthoDBiEOG092C2SAZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30200-MONOMER.

Miscellaneous databases

PROiP24384.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP22_YEAST
AccessioniPrimary (citable) accession number: P24384
Secondary accession number(s): D3DLR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.