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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

Gene

PRP22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts late in the splicing of pre-mRNA. Mediates the release of the spliced mRNA from spliceosomes.

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi506 – 513ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: GO_Central

GO - Biological processi

  • generation of catalytic spliceosome for second transesterification step Source: SGD
  • spliceosomal complex disassembly Source: SGD

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30200-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 (EC:3.6.4.13)
Gene namesi
Name:PRP22
Ordered Locus Names:YER013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER013W.
SGDiS000000815. PRP22.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551351 – 1145Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22Add BLAST1145

Proteomic databases

MaxQBiP24384.
PRIDEiP24384.

PTM databases

iPTMnetiP24384.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi36745. 333 interactors.
DIPiDIP-655N.
IntActiP24384. 3 interactors.
MINTiMINT-648209.
STRINGi4932.YER013W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MQ0electron microscopy4.17V1-1145[»]
ProteinModelPortaliP24384.
SMRiP24384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 250S1 motifPROSITE-ProRule annotationAdd BLAST73
Domaini493 – 656Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST164
Domaini678 – 854Helicase C-terminalPROSITE-ProRule annotationAdd BLAST177

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi603 – 606DEAH box4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00900000140898.
HOGENOMiHOG000175261.
InParanoidiP24384.
KOiK12818.
OMAiIQKAICS.
OrthoDBiEOG092C2SAZ.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
PfamiView protein in Pfam
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

P24384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE
60 70 80 90 100
DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDVN LDDPVVTEFV
110 120 130 140 150
LSILNKSKSI TEFQEQLNLM QSGLDNETIF KIYQIASPPV MKEEVSVLPS
160 170 180 190 200
TKIPAKIEAK IEEEVQKIES LDPSPVLHKV YEGKVRNITT FGCFVQIFGT
210 220 230 240 250
RMKNCDGLVH ISEMSDQRTL DPHDVVRQGQ HIFVEVIKIQ NNGKISLSMK
260 270 280 290 300
NIDQHSGEIR KRNTESVEDR GRSNDAHTSR NMKNKIKRRA LTSPERWEIR
310 320 330 340 350
QLIASGAASI DDYPELKDEI PINTSYLTAK RDDGSIVNGN TEKVDSKLEE
360 370 380 390 400
QQRDETDEID VELNTDDGPK FLKDQQVKGA KKYEMPKITK VPRGFMNRSA
410 420 430 440 450
INGSNAIRDH REEKLRKKRE IEQQIRKQQS FDDPTKNKKD SRNEIQMLKN
460 470 480 490 500
QLIVTEWEKN RMNESISYGK RTSLPISAQR QTLPVYAMRS ELIQAVRDNQ
510 520 530 540 550
FLVIVGETGS GKTTQITQYL DEEGFSNYGM IGCTQPRRVA AVSVAKRVAE
560 570 580 590 600
EVGCKVGHDV GYTIRFEDVT GPDTRIKYMT DGMLQREALL DPEMSKYSVI
610 620 630 640 650
MLDEAHERTV ATDVLFALLK KAAIKRPELK VIVTSATLNS AKFSEYFLNC
660 670 680 690 700
PIINIPGKTF PVEVLYSQTP QMDYIEAALD CVIDIHINEG PGDILVFLTG
710 720 730 740 750
QEEIDSCCEI LYDRVKTLGD SIGELLILPV YSALPSEIQS KIFEPTPKGS
760 770 780 790 800
RKVVFATNIA ETSITIDGIY YVVDPGFAKI NIYNARAGIE QLIVSPISQA
810 820 830 840 850
QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML
860 870 880 890 900
KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEGK LTNLGKEMSL
910 920 930 940 950
FPMDPTLSRS LLSSVDNQCS DEIVTIISML SVQNVFYRPK DRQLEADSKK
960 970 980 990 1000
AKFHHPYGDH LTLLNVYTRW QQANYSEQYC KTNFLHFRHL KRARDVKSQI
1010 1020 1030 1040 1050
SMIFKKIGLK LISCHSDPDL IRKTFVSGFF MNAAKRDSQV GYKTINGGTE
1060 1070 1080 1090 1100
VGIHPSSSLY GKEYEYVMYH SIVLTSREYM SQVTSIEPQW LLEVAPHFYK
1110 1120 1130 1140
AGDAESQSRK KAKIIPLHNK FAKDQNSWRL SSIRQSRERA LGIKR
Length:1,145
Mass (Da):130,011
Last modified:March 1, 1992 - v1
Checksum:iD92DBD620531B9A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58681 Genomic DNA. Translation: CAA41530.1.
U18778 Genomic DNA. Translation: AAB64546.1.
BK006939 Genomic DNA. Translation: DAA07664.1.
PIRiS13643.
RefSeqiNP_010929.3. NM_001178904.3.

Genome annotation databases

EnsemblFungiiYER013W; YER013W; YER013W.
GeneIDi856732.
KEGGisce:YER013W.

Similar proteinsi

Entry informationi

Entry nameiPRP22_YEAST
AccessioniPrimary (citable) accession number: P24384
Secondary accession number(s): D3DLR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 22, 2017
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names