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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

Gene

PRP22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts late in the splicing of pre-mRNA. Mediates the release of the spliced mRNA from spliceosomes.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi506 – 5138ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • generation of catalytic spliceosome for second transesterification step Source: SGD
  • spliceosomal complex disassembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30200-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 (EC:3.6.4.13)
Gene namesi
Name:PRP22
Ordered Locus Names:YER013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER013W.
SGDiS000000815. PRP22.

Subcellular locationi

GO - Cellular componenti

  • U2-type catalytic step 2 spliceosome Source: SGD
  • U2-type post-spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11451145Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22PRO_0000055135Add
BLAST

Proteomic databases

MaxQBiP24384.

PTM databases

iPTMnetiP24384.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi36745. 182 interactions.
DIPiDIP-655N.
IntActiP24384. 3 interactions.
MINTiMINT-648209.

Structurei

3D structure databases

ProteinModelPortaliP24384.
SMRiP24384. Positions 175-263, 480-1114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 25073S1 motifPROSITE-ProRule annotationAdd
BLAST
Domaini493 – 656164Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini678 – 854177Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi603 – 6064DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
InParanoidiP24384.
KOiK12818.
OMAiHSKEHHH.
OrthoDBiEOG7M98QW.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE
60 70 80 90 100
DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDVN LDDPVVTEFV
110 120 130 140 150
LSILNKSKSI TEFQEQLNLM QSGLDNETIF KIYQIASPPV MKEEVSVLPS
160 170 180 190 200
TKIPAKIEAK IEEEVQKIES LDPSPVLHKV YEGKVRNITT FGCFVQIFGT
210 220 230 240 250
RMKNCDGLVH ISEMSDQRTL DPHDVVRQGQ HIFVEVIKIQ NNGKISLSMK
260 270 280 290 300
NIDQHSGEIR KRNTESVEDR GRSNDAHTSR NMKNKIKRRA LTSPERWEIR
310 320 330 340 350
QLIASGAASI DDYPELKDEI PINTSYLTAK RDDGSIVNGN TEKVDSKLEE
360 370 380 390 400
QQRDETDEID VELNTDDGPK FLKDQQVKGA KKYEMPKITK VPRGFMNRSA
410 420 430 440 450
INGSNAIRDH REEKLRKKRE IEQQIRKQQS FDDPTKNKKD SRNEIQMLKN
460 470 480 490 500
QLIVTEWEKN RMNESISYGK RTSLPISAQR QTLPVYAMRS ELIQAVRDNQ
510 520 530 540 550
FLVIVGETGS GKTTQITQYL DEEGFSNYGM IGCTQPRRVA AVSVAKRVAE
560 570 580 590 600
EVGCKVGHDV GYTIRFEDVT GPDTRIKYMT DGMLQREALL DPEMSKYSVI
610 620 630 640 650
MLDEAHERTV ATDVLFALLK KAAIKRPELK VIVTSATLNS AKFSEYFLNC
660 670 680 690 700
PIINIPGKTF PVEVLYSQTP QMDYIEAALD CVIDIHINEG PGDILVFLTG
710 720 730 740 750
QEEIDSCCEI LYDRVKTLGD SIGELLILPV YSALPSEIQS KIFEPTPKGS
760 770 780 790 800
RKVVFATNIA ETSITIDGIY YVVDPGFAKI NIYNARAGIE QLIVSPISQA
810 820 830 840 850
QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML
860 870 880 890 900
KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEGK LTNLGKEMSL
910 920 930 940 950
FPMDPTLSRS LLSSVDNQCS DEIVTIISML SVQNVFYRPK DRQLEADSKK
960 970 980 990 1000
AKFHHPYGDH LTLLNVYTRW QQANYSEQYC KTNFLHFRHL KRARDVKSQI
1010 1020 1030 1040 1050
SMIFKKIGLK LISCHSDPDL IRKTFVSGFF MNAAKRDSQV GYKTINGGTE
1060 1070 1080 1090 1100
VGIHPSSSLY GKEYEYVMYH SIVLTSREYM SQVTSIEPQW LLEVAPHFYK
1110 1120 1130 1140
AGDAESQSRK KAKIIPLHNK FAKDQNSWRL SSIRQSRERA LGIKR
Length:1,145
Mass (Da):130,011
Last modified:March 1, 1992 - v1
Checksum:iD92DBD620531B9A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58681 Genomic DNA. Translation: CAA41530.1.
U18778 Genomic DNA. Translation: AAB64546.1.
BK006939 Genomic DNA. Translation: DAA07664.1.
PIRiS13643.
RefSeqiNP_010929.3. NM_001178904.3.

Genome annotation databases

EnsemblFungiiYER013W; YER013W; YER013W.
GeneIDi856732.
KEGGisce:YER013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58681 Genomic DNA. Translation: CAA41530.1.
U18778 Genomic DNA. Translation: AAB64546.1.
BK006939 Genomic DNA. Translation: DAA07664.1.
PIRiS13643.
RefSeqiNP_010929.3. NM_001178904.3.

3D structure databases

ProteinModelPortaliP24384.
SMRiP24384. Positions 175-263, 480-1114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36745. 182 interactions.
DIPiDIP-655N.
IntActiP24384. 3 interactions.
MINTiMINT-648209.

PTM databases

iPTMnetiP24384.

Proteomic databases

MaxQBiP24384.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER013W; YER013W; YER013W.
GeneIDi856732.
KEGGisce:YER013W.

Organism-specific databases

EuPathDBiFungiDB:YER013W.
SGDiS000000815. PRP22.

Phylogenomic databases

GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
InParanoidiP24384.
KOiK12818.
OMAiHSKEHHH.
OrthoDBiEOG7M98QW.

Enzyme and pathway databases

BioCyciYEAST:G3O-30200-MONOMER.

Miscellaneous databases

NextBioi982846.
PROiP24384.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes."
    Company M., Arenas J., Abelson J.
    Nature 349:487-493(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP22_YEAST
AccessioniPrimary (citable) accession number: P24384
Secondary accession number(s): D3DLR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 11, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.