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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

Gene

PRP22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts late in the splicing of pre-mRNA. Mediates the release of the spliced mRNA from spliceosomes.1 Publication

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi506 – 513ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: GO_Central

GO - Biological processi

  • generation of catalytic spliceosome for second transesterification step Source: SGD
  • spliceosomal complex disassembly Source: SGD

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30200-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 (EC:3.6.4.131 Publication)
Gene namesi
Name:PRP22
Ordered Locus Names:YER013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER013W
SGDiS000000815 PRP22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551351 – 1145Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22Add BLAST1145

Proteomic databases

MaxQBiP24384
PaxDbiP24384
PRIDEiP24384

PTM databases

iPTMnetiP24384

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi36745, 335 interactors
ComplexPortaliCPX-1651 PRP19-associated complex
DIPiDIP-655N
IntActiP24384, 3 interactors
MINTiP24384
STRINGi4932.YER013W

Structurei

Secondary structure

11145
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi477 – 480Combined sources4
Helixi485 – 488Combined sources4
Helixi489 – 497Combined sources9
Beta strandi500 – 505Combined sources6
Helixi512 – 524Combined sources13
Beta strandi531 – 536Combined sources6
Helixi538 – 552Combined sources15
Beta strandi558 – 564Combined sources7
Beta strandi567 – 569Combined sources3
Beta strandi576 – 580Combined sources5
Helixi581 – 590Combined sources10
Beta strandi598 – 602Combined sources5
Helixi610 – 625Combined sources16
Beta strandi630 – 636Combined sources7
Helixi641 – 646Combined sources6
Beta strandi652 – 654Combined sources3
Beta strandi663 – 665Combined sources3
Helixi674 – 687Combined sources14
Beta strandi692 – 697Combined sources6
Helixi701 – 717Combined sources17
Beta strandi725 – 730Combined sources6
Helixi736 – 739Combined sources4
Helixi740 – 743Combined sources4
Beta strandi750 – 756Combined sources7
Helixi758 – 760Combined sources3
Beta strandi769 – 774Combined sources6
Beta strandi777 – 784Combined sources8
Turni785 – 788Combined sources4
Beta strandi789 – 796Combined sources8
Helixi799 – 807Combined sources9
Helixi808 – 810Combined sources3
Beta strandi811 – 814Combined sources4
Beta strandi816 – 821Combined sources6
Helixi823 – 828Combined sources6
Helixi837 – 840Combined sources4
Helixi846 – 853Combined sources8
Beta strandi856 – 858Combined sources3
Turni859 – 861Combined sources3
Helixi869 – 881Combined sources13
Beta strandi889 – 891Combined sources3
Helixi893 – 899Combined sources7
Beta strandi901 – 903Combined sources3
Helixi905 – 916Combined sources12
Helixi920 – 931Combined sources12
Beta strandi940 – 942Combined sources3
Helixi943 – 953Combined sources11
Helixi960 – 972Combined sources13
Helixi977 – 982Combined sources6
Helixi987 – 1005Combined sources19
Helixi1018 – 1029Combined sources12
Beta strandi1030 – 1037Combined sources8
Turni1038 – 1040Combined sources3
Beta strandi1041 – 1044Combined sources4
Turni1045 – 1047Combined sources3
Beta strandi1060 – 1062Combined sources3
Beta strandi1065 – 1077Combined sources13
Beta strandi1079 – 1086Combined sources8
Helixi1088 – 1094Combined sources7
Turni1096 – 1098Combined sources3
Turni1108 – 1110Combined sources3
Turni1125 – 1128Combined sources4
Helixi1130 – 1140Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MQ0electron microscopy4.17V1-1145[»]
6BK8electron microscopy3.30P1-1145[»]
6EXNelectron microscopy3.70V1-1145[»]
ProteinModelPortaliP24384
SMRiP24384
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 250S1 motifPROSITE-ProRule annotationAdd BLAST73
Domaini493 – 656Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST164
Domaini678 – 854Helicase C-terminalPROSITE-ProRule annotationAdd BLAST177

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi603 – 606DEAH box4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
InParanoidiP24384
KOiK12818
OMAiIQKAICS
OrthoDBiEOG092C2SAZ

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
IPR022967 S1_dom
IPR003029 S1_domain
PfamiView protein in Pfam
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
PF00575 S1, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

P24384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE
60 70 80 90 100
DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDVN LDDPVVTEFV
110 120 130 140 150
LSILNKSKSI TEFQEQLNLM QSGLDNETIF KIYQIASPPV MKEEVSVLPS
160 170 180 190 200
TKIPAKIEAK IEEEVQKIES LDPSPVLHKV YEGKVRNITT FGCFVQIFGT
210 220 230 240 250
RMKNCDGLVH ISEMSDQRTL DPHDVVRQGQ HIFVEVIKIQ NNGKISLSMK
260 270 280 290 300
NIDQHSGEIR KRNTESVEDR GRSNDAHTSR NMKNKIKRRA LTSPERWEIR
310 320 330 340 350
QLIASGAASI DDYPELKDEI PINTSYLTAK RDDGSIVNGN TEKVDSKLEE
360 370 380 390 400
QQRDETDEID VELNTDDGPK FLKDQQVKGA KKYEMPKITK VPRGFMNRSA
410 420 430 440 450
INGSNAIRDH REEKLRKKRE IEQQIRKQQS FDDPTKNKKD SRNEIQMLKN
460 470 480 490 500
QLIVTEWEKN RMNESISYGK RTSLPISAQR QTLPVYAMRS ELIQAVRDNQ
510 520 530 540 550
FLVIVGETGS GKTTQITQYL DEEGFSNYGM IGCTQPRRVA AVSVAKRVAE
560 570 580 590 600
EVGCKVGHDV GYTIRFEDVT GPDTRIKYMT DGMLQREALL DPEMSKYSVI
610 620 630 640 650
MLDEAHERTV ATDVLFALLK KAAIKRPELK VIVTSATLNS AKFSEYFLNC
660 670 680 690 700
PIINIPGKTF PVEVLYSQTP QMDYIEAALD CVIDIHINEG PGDILVFLTG
710 720 730 740 750
QEEIDSCCEI LYDRVKTLGD SIGELLILPV YSALPSEIQS KIFEPTPKGS
760 770 780 790 800
RKVVFATNIA ETSITIDGIY YVVDPGFAKI NIYNARAGIE QLIVSPISQA
810 820 830 840 850
QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML
860 870 880 890 900
KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEGK LTNLGKEMSL
910 920 930 940 950
FPMDPTLSRS LLSSVDNQCS DEIVTIISML SVQNVFYRPK DRQLEADSKK
960 970 980 990 1000
AKFHHPYGDH LTLLNVYTRW QQANYSEQYC KTNFLHFRHL KRARDVKSQI
1010 1020 1030 1040 1050
SMIFKKIGLK LISCHSDPDL IRKTFVSGFF MNAAKRDSQV GYKTINGGTE
1060 1070 1080 1090 1100
VGIHPSSSLY GKEYEYVMYH SIVLTSREYM SQVTSIEPQW LLEVAPHFYK
1110 1120 1130 1140
AGDAESQSRK KAKIIPLHNK FAKDQNSWRL SSIRQSRERA LGIKR
Length:1,145
Mass (Da):130,011
Last modified:March 1, 1992 - v1
Checksum:iD92DBD620531B9A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58681 Genomic DNA Translation: CAA41530.1
U18778 Genomic DNA Translation: AAB64546.1
BK006939 Genomic DNA Translation: DAA07664.1
PIRiS13643
RefSeqiNP_010929.3, NM_001178904.3

Genome annotation databases

EnsemblFungiiYER013W; YER013W; YER013W
GeneIDi856732
KEGGisce:YER013W

Similar proteinsi

Entry informationi

Entry nameiPRP22_YEAST
AccessioniPrimary (citable) accession number: P24384
Secondary accession number(s): D3DLR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 20, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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