P24383 (WNT7A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-7a

Gene names

Name:	Wnt7a
Synonyms:	Wnt-7a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Ref.5
Subunit structure	Interacts with PORCN. Ref.6
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Palmitoylation at Ser-206 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-73. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Involvement in disease	Male mice lacking Wnt7a fail to undergo regression of the Mullerian duct as a result of the absence of the receptor for Mullerian-inhibiting substance. Wnt7a deficient females are infertile because of abnormal development of the oviduct and uterus, both of which are mullerian duct derivatives.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt signaling pathway involved in wound healing, spreading of epidermal cells Inferred from electronic annotation. Source: Ensembl asymmetric protein localization Inferred from direct assay PubMed 19497282. Source: MGI canonical Wnt signaling pathway Inferred from genetic interaction PubMed 19023080. Source: MGI cartilage condensation Inferred from electronic annotation. Source: Ensembl cartilage development Inferred from direct assay PubMed 16826533. Source: BHF-UCL cell fate commitment Inferred from Biological aspect of Ancestor. Source: RefGenome cell proliferation in forebrain Inferred from electronic annotation. Source: Ensembl cell-cell signaling Traceable author statement PubMed 9889131. Source: MGI cellular response to transforming growth factor beta stimulus Inferred from electronic annotation. Source: Ensembl central nervous system vasculogenesis Inferred from genetic interaction PubMed 19023080. Source: MGI cerebellar granule cell differentiation Inferred from direct assay PubMed 9405095. Source: MGI chondrocyte differentiation Inferred from electronic annotation. Source: Ensembl dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 7885472PubMed 9362463. Source: MGI embryonic axis specification Inferred from electronic annotation. Source: Ensembl embryonic digit morphogenesis Inferred from electronic annotation. Source: Ensembl embryonic forelimb morphogenesis Inferred from genetic interaction PubMed 15880584PubMed 9362463. Source: MGI embryonic hindlimb morphogenesis Inferred from genetic interaction PubMed 15880584. Source: MGI embryonic limb morphogenesis Inferred from genetic interaction PubMed 15242796. Source: MGI establishment of cell polarity Inferred from direct assay PubMed 19497282. Source: MGI limb development Inferred from direct assay PubMed 16826533. Source: BHF-UCL negative regulation of apoptotic process Inferred from mutant phenotype PubMed 15070830. Source: MGI negative regulation of neurogenesis Inferred from electronic annotation. Source: Ensembl neurotransmitter secretion Inferred from genetic interaction PubMed 16818724. Source: MGI non-canonical Wnt signaling pathway Inferred from genetic interaction PubMed 19497282. Source: MGI organ morphogenesis Traceable author statement PubMed 9889131. Source: MGI oviduct development Inferred from mutant phenotype PubMed 14695376. Source: UniProtKB palate development Inferred from electronic annotation. Source: Ensembl positive regulation of JNK cascade Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of canonical Wnt signaling pathway Inferred from direct assay PubMed 12937339. Source: UniProtKB positive regulation of epithelial cell proliferation involved in wound healing Inferred from electronic annotation. Source: Ensembl positive regulation of synapse assembly Inferred from direct assay PubMed 9405095. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Ensembl positive regulation of transcription, DNA-templated Inferred from direct assay PubMed 12937339. Source: UniProtKB regulation of axon diameter Inferred from direct assay PubMed 9405095. Source: MGI regulation of axonogenesis Inferred from direct assay PubMed 9405095. Source: MGI regulation of cell proliferation Inferred from mutant phenotype PubMed 15070830. Source: MGI response to estradiol Inferred from electronic annotation. Source: Ensembl response to estrogen Inferred from mutant phenotype PubMed 15070830. Source: MGI satellite cell activation Inferred from direct assay PubMed 19497282. Source: MGI satellite cell maintenance involved in skeletal muscle regeneration Inferred from direct assay PubMed 19497282. Source: MGI signal transduction Traceable author statement PubMed 9889131. Source: MGI somatic stem cell division Inferred from direct assay PubMed 19497282. Source: MGI somatic stem cell maintenance Inferred from mutant phenotype PubMed 19497282. Source: MGI synapse organization Inferred from mutant phenotype PubMed 16818724. Source: MGI uterus development Inferred from mutant phenotype PubMed 15070830PubMed 15073149. Source: MGI uterus morphogenesis Inferred from mutant phenotype PubMed 14695376. Source: UniProtKB
Cellular_component	cell surface Inferred from direct assay PubMed 8167409. Source: BHF-UCL endoplasmic reticulum lumen Traceable author statement. Source: Reactome extracellular matrix Inferred from direct assay PubMed 8167409. Source: MGI extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	frizzled binding Inferred from physical interaction PubMed 19497282. Source: MGI receptor binding Traceable author statement PubMed 9889131. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 349	318	Protein Wnt-7a		PRO_0000041443
Amino acid modifications
	Lipidation	73	1	S-palmitoyl cysteine By similarity
	Lipidation	206	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	143	1	R → W in AAA40570. Ref.1
	Sequence conflict	273	1	K → L in AAA40570. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24383 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: C36ACDF7930790A1	FASTA	349	38,974
	10 20 30 40 50 60 MTRKARRCLG HLFLSLGIVY LRIGGFSSVV ALGASIICNK IPGLAPRQRA ICQSRPDAII 70 80 90 100 110 120 VIGEGSQMGL DECQFQFRNG RWNCSALGER TVFGKELKVG SREAAFTYAI IAAGVAHAIT 130 140 150 160 170 180 AACTQGNLSD CGCDKEKQGQ YHRDEGWKWG GCSADIRYGI GFAKVFVDAR EIKQNARTLM 190 200 210 220 230 240 NLHNNEAGRK ILEENMKLEC KCHGVSGSCT TKTCWTTLPQ FRELGYVLKD KYNEAVHVEP 250 260 270 280 290 300 VRASRNKRPT FLKIKKPLSY RKPMDTDLVY IEKSPNYCEE DPVTGSVGTQ GRACNKTAPQ 310 320 330 340 ASGCDLMCCG RGYNTHQYAR VWQCNCKFHW CCYVKCNTCS ERTEMYTCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6.
[5]	"Sexually dimorphic development of the mammalian reproductive tract requires Wnt-7a." Parr B.A., McMahon A.P. Nature 395:707-710(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M89801 mRNA. Translation: AAA40570.1. AK004683 mRNA. Translation: BAB23470.1. CH466523 Genomic DNA. Translation: EDK99298.1. BC049093 mRNA. Translation: AAH49093.2. BC057586 mRNA. Translation: AAH57586.1.
CCDS	CCDS39568.1.
PIR	G36470.
RefSeq	NP_033553.2. NM_009527.3.
UniGene	Mm.56964.
3D structure databases
ProteinModelPortal	P24383.
SMR	P24383. Positions 61-278.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	204580. 4 interactions.
PTM databases
PhosphoSite	P24383.
Proteomic databases
PRIDE	P24383.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032180; ENSMUSP00000032180; ENSMUSG00000030093.
GeneID	22421.
KEGG	mmu:22421.
UCSC	uc009cxz.1. mouse.
Organism-specific databases
CTD	7476.
MGI	MGI:98961. Wnt7a.
Phylogenomic databases
eggNOG	NOG284879.
GeneTree	ENSGT00740000115016.
HOGENOM	HOG000039528.
HOVERGEN	HBG001595.
InParanoid	Q9DBY3.
KO	K00572.
OMA	HISGCDL.
OrthoDB	EOG7C8GJ8.
TreeFam	TF105310.
Enzyme and pathway databases
Reactome	REACT_188257. Signal Transduction.
Gene expression databases
Bgee	P24383.
CleanEx	MM_WNT7A.
Genevestigator	P24383.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013300. Wnt7. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01891. WNT7PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302853.
PRO	P24383.
SOURCE	Search...

Entry information

Entry name

WNT7A_MOUSE

Accession

Primary (citable) accession number: P24383
Secondary accession number(s): Q80VH3, Q9DBY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	June 28, 2011
Last modified:	July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program