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Protein

Protein Wnt-7a

Gene

Wnt7a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors (PubMed:18230341, PubMed:20530549). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341, PubMed:23629626). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:7885472, PubMed:9790192, PubMed:9769174). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (PubMed:9790192). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (PubMed:23629626). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (PubMed:23629626). Promotes formation of synapses via its interaction with FZD5 (PubMed:20530549).6 Publications

Miscellaneous

Male mice lacking Wnt7a fail to undergo regression of the Mullerian duct as a result of the absence of the receptor for Mullerian-inhibiting substance. In males, mature sperm fills the vas deferens, but sperm exit is blocked due to the persistence of the Mullerian duct, causing male sterility. Wnt7a deficient females are infertile because of abnormal development of the oviduct and uterus, both of which are Mullerian duct derivatives.1 Publication

GO - Molecular functioni

  • cytokine activity Source: MGI
  • frizzled binding Source: MGI
  • receptor binding Source: MGI
  • receptor ligand activity Source: MGI

GO - Biological processi

  • angiogenesis Source: MGI
  • animal organ morphogenesis Source: MGI
  • asymmetric protein localization involved in cell fate determination Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • cartilage condensation Source: MGI
  • cartilage development Source: BHF-UCL
  • cell-cell signaling Source: MGI
  • cell fate commitment Source: GO_Central
  • cell proliferation in forebrain Source: MGI
  • cellular response to transforming growth factor beta stimulus Source: Ensembl
  • central nervous system vasculogenesis Source: MGI
  • cerebellar granule cell differentiation Source: MGI
  • chondrocyte differentiation Source: MGI
  • dendritic spine morphogenesis Source: ParkinsonsUK-UCL
  • dorsal/ventral pattern formation Source: MGI
  • embryonic axis specification Source: MGI
  • embryonic digit morphogenesis Source: MGI
  • embryonic forelimb morphogenesis Source: MGI
  • embryonic hindlimb morphogenesis Source: MGI
  • embryonic limb morphogenesis Source: MGI
  • establishment of cell polarity Source: MGI
  • limb development Source: BHF-UCL
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of neurogenesis Source: MGI
  • neurotransmitter secretion Source: MGI
  • non-canonical Wnt signaling pathway Source: MGI
  • oviduct development Source: UniProtKB
  • palate development Source: MGI
  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • positive regulation of cellular protein metabolic process Source: MGI
  • positive regulation of endothelial cell migration Source: MGI
  • positive regulation of epithelial cell proliferation involved in wound healing Source: MGI
  • positive regulation of excitatory postsynaptic potential Source: ParkinsonsUK-UCL
  • positive regulation of excitatory synapse assembly Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of JNK cascade Source: Ensembl
  • positive regulation of synapse assembly Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of axon diameter Source: MGI
  • regulation of axonogenesis Source: MGI
  • regulation of cell proliferation Source: MGI
  • response to estradiol Source: Ensembl
  • response to estrogen Source: MGI
  • signal transduction Source: MGI
  • skeletal muscle satellite cell activation Source: MGI
  • skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration Source: MGI
  • somatic stem cell division Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • stem cell development Source: MGI
  • synapse organization Source: MGI
  • uterus development Source: MGI
  • uterus morphogenesis Source: UniProtKB
  • Wnt signaling pathway Source: ParkinsonsUK-UCL
  • Wnt signaling pathway involved in wound healing, spreading of epidermal cells Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-7a
Gene namesi
Name:Wnt7a
Synonyms:Wnt-7a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98961. Wnt7a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Wnt7a cause the postaxial hemimelia (px) phenotype that is characterized by limb patterning defects accompanied by Mullerian duct-associated sterility in both sexes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000004144332 – 349Protein Wnt-7aAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 84By similarity
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi123 ↔ 131By similarity
Glycosylationi127N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi133 ↔ 152By similarity
Disulfide bondi200 ↔ 214By similarity
Disulfide bondi202 ↔ 209By similarity
Lipidationi206O-palmitoleoyl serine; by PORCNBy similarity1
Disulfide bondi294 ↔ 309By similarity
Glycosylationi295N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi324 ↔ 339By similarity
Disulfide bondi326 ↔ 336By similarity
Disulfide bondi331 ↔ 332By similarity

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiP24383.
PRIDEiP24383.

PTM databases

PhosphoSitePlusiP24383.

Expressioni

Developmental stagei

Detected in the flanking ectoderm of the trunk prior to limb outgrowth. First detected in the presumptive forelimb region at 8.75 dpc, and in the presumptive hindlimb region at 9.25 dpc. Uniformly distributed throughout the dorsal limb ectoderm during the initial stages of limb-bud outgrowth (9.25 dpc for the forelimbs, 9.75 dpc for the hindlimbs).1 Publication

Gene expression databases

BgeeiENSMUSG00000030093.
CleanExiMM_WNT7A.
GenevisibleiP24383. MM.

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids (By similarity). Interacts with FZD5 (PubMed:18230341, PubMed:20530549). Interacts with PORCN (PubMed:10866835).By similarity3 Publications

GO - Molecular functioni

  • cytokine activity Source: MGI
  • frizzled binding Source: MGI
  • receptor binding Source: MGI
  • receptor ligand activity Source: MGI

Protein-protein interaction databases

BioGridi204580. 4 interactors.
STRINGi10090.ENSMUSP00000032180.

Structurei

3D structure databases

ProteinModelPortaliP24383.
SMRiP24383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039528.
HOVERGENiHBG001595.
InParanoidiP24383.
KOiK00572.
OMAiYIDKSPN.
OrthoDBiEOG091G0OFF.
TreeFamiTF105310.

Family and domain databases

InterProiView protein in InterPro
IPR005817. Wnt.
IPR013300. Wnt7.
IPR018161. Wnt_CS.
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiView protein in Pfam
PF00110. wnt. 1 hit.
PRINTSiPR01891. WNT7PROTEIN.
PR01349. WNTPROTEIN.
SMARTiView protein in SMART
SM00097. WNT1. 1 hit.
PROSITEiView protein in PROSITE
PS00246. WNT1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRKARRCLG HLFLSLGIVY LRIGGFSSVV ALGASIICNK IPGLAPRQRA
60 70 80 90 100
ICQSRPDAII VIGEGSQMGL DECQFQFRNG RWNCSALGER TVFGKELKVG
110 120 130 140 150
SREAAFTYAI IAAGVAHAIT AACTQGNLSD CGCDKEKQGQ YHRDEGWKWG
160 170 180 190 200
GCSADIRYGI GFAKVFVDAR EIKQNARTLM NLHNNEAGRK ILEENMKLEC
210 220 230 240 250
KCHGVSGSCT TKTCWTTLPQ FRELGYVLKD KYNEAVHVEP VRASRNKRPT
260 270 280 290 300
FLKIKKPLSY RKPMDTDLVY IEKSPNYCEE DPVTGSVGTQ GRACNKTAPQ
310 320 330 340
ASGCDLMCCG RGYNTHQYAR VWQCNCKFHW CCYVKCNTCS ERTEMYTCK
Length:349
Mass (Da):38,974
Last modified:June 28, 2011 - v2
Checksum:iC36ACDF7930790A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143R → W in AAA40570 (PubMed:2279700).Curated1
Sequence conflicti273K → L in AAA40570 (PubMed:2279700).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89801 mRNA. Translation: AAA40570.1.
AK004683 mRNA. Translation: BAB23470.1.
CH466523 Genomic DNA. Translation: EDK99298.1.
BC049093 mRNA. Translation: AAH49093.2.
BC057586 mRNA. Translation: AAH57586.1.
CCDSiCCDS39568.1.
PIRiG36470.
RefSeqiNP_033553.2. NM_009527.3.
UniGeneiMm.56964.

Genome annotation databases

EnsembliENSMUST00000032180; ENSMUSP00000032180; ENSMUSG00000030093.
GeneIDi22421.
KEGGimmu:22421.
UCSCiuc009cxz.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiWNT7A_MOUSE
AccessioniPrimary (citable) accession number: P24383
Secondary accession number(s): Q80VH3, Q9DBY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 28, 2011
Last modified: March 28, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome