ID   IPOU_DROME              Reviewed;         396 AA.
AC   P24350; O77214; Q26465; Q59E44; Q59E45; Q8IR36; Q9VXW1;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 3.
DT   24-JAN-2024, entry version 195.
DE   RecName: Full=Inhibitory POU protein;
DE            Short=I-POU;
DE   AltName: Full=Abnormal chemosensory jump 6 protein;
GN   Name=acj6; Synonyms=Ipou; ORFNames=CG9151;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), AND TISSUE SPECIFICITY.
RX   PubMed=1673230; DOI=10.1038/350577a0;
RA   Treacy M.N., He X., Rosenfeld M.G.;
RT   "I-POU: a POU-domain protein that inhibits neuron-specific gene
RT   activation.";
RL   Nature 350:577-584(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND TISSUE SPECIFICITY.
RC   STRAIN=Canton-S;
RX   PubMed=10069339; DOI=10.1016/s0896-6273(00)81094-6;
RA   Clyne P.J., Certel S.J., de Bruyne M., Zaslavsky L., Johnson W.A.,
RA   Carlson J.R.;
RT   "The odor specificities of a subset of olfactory receptor neurons are
RT   governed by Acj6, a POU-domain transcription factor.";
RL   Neuron 22:339-347(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [4]
RP   GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 318-327 (ISOFORMS B/C/D), AND
RP   FUNCTION.
RX   PubMed=1346754; DOI=10.1016/0092-8674(92)90186-g;
RA   Treacy M.N., Neilson L.I., Turner E.E., He X., Rosenfeld M.G.;
RT   "Twin of I-POU: a two amino acid difference in the I-POU homeodomain
RT   distinguishes an activator from an inhibitor of transcription.";
RL   Cell 68:491-505(1992).
RN   [6]
RP   DNA-BINDING.
RX   PubMed=8986770; DOI=10.1073/pnas.93.26.15097;
RA   Turner E.E.;
RT   "Similar DNA recognition properties of alternatively spliced Drosophila POU
RT   factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:15097-15101(1996).
CC   -!- FUNCTION: Modulates gene transcription; simultaneously generates both a
CC       specific activator and an inhibitor of gene transcription, capable of
CC       modulating two distinct regulatory programs during neural development.
CC       Has a role in olfactory behavior. {ECO:0000269|PubMed:10069339,
CC       ECO:0000269|PubMed:1346754}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=A; Synonyms=TI-POU, Twin-of-I-POU;
CC         IsoId=P24350-2; Sequence=Displayed;
CC       Name=C; Synonyms=I-POU;
CC         IsoId=P24350-1; Sequence=VSP_018342, VSP_018343, VSP_002342;
CC       Name=B;
CC         IsoId=P24350-3; Sequence=VSP_018342, VSP_002342;
CC       Name=D;
CC         IsoId=P24350-4; Sequence=VSP_002342;
CC   -!- TISSUE SPECIFICITY: Coexpressed with vvl in overlapping subsets of
CC       neurons in the embryonic central nervous system. Expressed in olfactory
CC       neurons. {ECO:0000269|PubMed:10069339, ECO:0000269|PubMed:1673230}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-4
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought that the I-POU homeobox is unable to
CC       bind DNA because it lacks two N-terminal basic residues.
CC       {ECO:0000305|PubMed:1673230}.
CC   -!- CAUTION: Was originally thought to interact with vvl.
CC       {ECO:0000305|PubMed:1673230}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA41342.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X58436; CAA41342.1; ALT_FRAME; mRNA.
DR   EMBL; AF086816; AAC35369.1; -; mRNA.
DR   EMBL; AE014298; AAF48447.2; -; Genomic_DNA.
DR   EMBL; AE014298; AAN09663.1; -; Genomic_DNA.
DR   EMBL; AE014298; AAX52497.1; -; Genomic_DNA.
DR   EMBL; AE014298; AAX52498.1; -; Genomic_DNA.
DR   EMBL; AH003854; AAB21441.1; -; Genomic_DNA.
DR   PIR; A42089; A42089.
DR   PIR; S14795; S14795.
DR   RefSeq; NP_001014743.1; NM_001014743.3. [P24350-4]
DR   RefSeq; NP_001014744.1; NM_001014744.2. [P24350-1]
DR   RefSeq; NP_524876.1; NM_080137.3. [P24350-2]
DR   RefSeq; NP_727845.1; NM_167438.2. [P24350-3]
DR   AlphaFoldDB; P24350; -.
DR   SMR; P24350; -.
DR   BioGRID; 70572; 5.
DR   STRING; 7227.FBpp0073832; -.
DR   PaxDb; 7227-FBpp0073832; -.
DR   DNASU; 47080; -.
DR   EnsemblMetazoa; FBtr0074015; FBpp0073832; FBgn0000028. [P24350-2]
DR   EnsemblMetazoa; FBtr0100326; FBpp0099732; FBgn0000028. [P24350-1]
DR   EnsemblMetazoa; FBtr0100328; FBpp0099734; FBgn0000028. [P24350-4]
DR   EnsemblMetazoa; FBtr0290063; FBpp0288502; FBgn0000028. [P24350-3]
DR   GeneID; 47080; -.
DR   KEGG; dme:Dmel_CG9151; -.
DR   AGR; FB:FBgn0000028; -.
DR   CTD; 47080; -.
DR   FlyBase; FBgn0000028; acj6.
DR   VEuPathDB; VectorBase:FBgn0000028; -.
DR   eggNOG; KOG1168; Eukaryota.
DR   GeneTree; ENSGT00940000169264; -.
DR   InParanoid; P24350; -.
DR   OMA; HRRKMEN; -.
DR   OrthoDB; 4250502at2759; -.
DR   PhylomeDB; P24350; -.
DR   Reactome; R-DME-6804759; Regulation of TP53 Activity through Association with Co-factors.
DR   BioGRID-ORCS; 47080; 0 hits in 3 CRISPR screens.
DR   ChiTaRS; acj6; fly.
DR   GenomeRNAi; 47080; -.
DR   PRO; PR:P24350; -.
DR   Proteomes; UP000000803; Chromosome X.
DR   Bgee; FBgn0000028; Expressed in brain and 22 other cell types or tissues.
DR   ExpressionAtlas; P24350; baseline and differential.
DR   GO; GO:0005634; C:nucleus; ISS:FlyBase.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:FlyBase.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003714; F:transcription corepressor activity; IGI:FlyBase.
DR   GO; GO:0007411; P:axon guidance; IMP:FlyBase.
DR   GO; GO:0007636; P:chemosensory jump behavior; IMP:FlyBase.
DR   GO; GO:0070983; P:dendrite guidance; IMP:FlyBase.
DR   GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR   GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IDA:FlyBase.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0008039; P:synaptic target recognition; IMP:FlyBase.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636:SF70; INHIBITORY POU PROTEIN; 1.
DR   PANTHER; PTHR11636; POU DOMAIN; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
DR   Genevisible; P24350; DM.
PE   1: Evidence at protein level;
KW   Activator; Alternative splicing; DNA-binding; Homeobox; Nucleus; Olfaction;
KW   Reference proteome; Repressor; Sensory transduction; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..396
FT                   /note="Inhibitory POU protein"
FT                   /id="PRO_0000100775"
FT   DOMAIN          222..299
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        320..377
FT                   /note="Homeobox; atypical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          302..328
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           86..95
FT                   /note="POU-IV box"
FT   VAR_SEQ         54..72
FT                   /note="Missing (in isoform B and isoform C)"
FT                   /evidence="ECO:0000303|PubMed:1673230"
FT                   /id="VSP_018342"
FT   VAR_SEQ         132..138
FT                   /note="Missing (in isoform C)"
FT                   /evidence="ECO:0000303|PubMed:1673230"
FT                   /id="VSP_018343"
FT   VAR_SEQ         323..324
FT                   /note="Missing (in isoform B, isoform C and isoform D)"
FT                   /evidence="ECO:0000303|PubMed:1673230"
FT                   /id="VSP_002342"
FT   CONFLICT        166
FT                   /note="L -> V (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        216
FT                   /note="A -> P (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        266
FT                   /note="L -> V (in Ref. 1; CAA41342)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   396 AA;  43704 MW;  AEA80A7EF633B30D CRC64;
     MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD SLESRLLEDA
     SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA QHVHSQMPSQ IKHDVMYHHH
     SMSGPPQRPL QENPFSRQMH HSMDQLDMLD PTGSMTTLAP ISESPLTPTH QHLHGSYHSM
     NHMMSHHHPG TLSGHTGGHH GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR
     RIKLGVTQAD VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA
     QAKNKRRDPD APSVLPAGEK KRKRTSIAAP EKRSLEAYFA VQPRPSGEKI AAIAEKLDLK
     KNVVRVWFCN QRQKQKRIVS SVTPSMTGHG SAGFGY
//