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P24350

- IPOU_DROME

UniProt

P24350 - IPOU_DROME

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Protein

Inhibitory POU protein

Gene
acj6, Ipou, CG9151
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Modulates gene transcription; simultaneously generates both a specific activator and an inhibitor of gene transcription, capable of modulating two distinct regulatory programs during neural development. Has a role in olfactory behavior.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi320 – 37758Homeobox; atypical1 PublicationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: FlyBase
  3. transcription corepressor activity Source: FlyBase

GO - Biological processi

  1. axon guidance Source: FlyBase
  2. chemosensory jump behavior Source: FlyBase
  3. dendrite guidance Source: FlyBase
  4. dendrite morphogenesis Source: FlyBase
  5. detection of chemical stimulus involved in sensory perception of smell Source: FlyBase
  6. positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  7. synaptic target recognition Source: FlyBase
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Olfaction, Sensory transduction, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory POU protein
Short name:
I-POU
Alternative name(s):
Abnormal chemosensory jump 6 protein
Gene namesi
Name:acj6
Synonyms:Ipou
ORF Names:CG9151
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000028. acj6.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Inhibitory POU proteinPRO_0000100775Add
BLAST

Proteomic databases

PaxDbiP24350.

Expressioni

Tissue specificityi

Coexpressed with vvl in overlapping subsets of neurons in the embryonic central nervous system. Expressed in olfactory neurons.2 Publications

Gene expression databases

BgeeiP24350.

Interactioni

Protein-protein interaction databases

BioGridi70572. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP24350.
SMRiP24350. Positions 226-377.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini222 – 29978POU-specificAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi86 – 9510POU-IV box

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG289030.
GeneTreeiENSGT00750000117482.
InParanoidiP24350.
KOiK09366.
OMAiGHSAVHH.
OrthoDBiEOG7RV9G7.
PhylomeDBiP24350.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015584. POU4-related.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF10. PTHR11636:SF10. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform A (identifier: P24350-2) [UniParc]FASTAAdd to Basket

Also known as: TI-POU, Twin-of-I-POU

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD    50
SLESRLLEDA SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA 100
QHVHSQMPSQ IKHDVMYHHH SMSGPPQRPL QENPFSRQMH HSMDQLDMLD 150
PTGSMTTLAP ISESPLTPTH QHLHGSYHSM NHMMSHHHPG TLSGHTGGHH 200
GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR RIKLGVTQAD 250
VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA 300
QAKNKRRDPD APSVLPAGEK KRKRTSIAAP EKRSLEAYFA VQPRPSGEKI 350
AAIAEKLDLK KNVVRVWFCN QRQKQKRIVS SVTPSMTGHG SAGFGY 396
Length:396
Mass (Da):43,704
Last modified:May 16, 2006 - v3
Checksum:iAEA80A7EF633B30D
GO
Isoform C (identifier: P24350-1) [UniParc]FASTAAdd to Basket

Also known as: I-POU

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     132-138: Missing.
     323-324: Missing.

Show »
Length:368
Mass (Da):40,415
Checksum:i10F6A5623E79F148
GO
Isoform B (identifier: P24350-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     323-324: Missing.

Note: No experimental confirmation available.

Show »
Length:375
Mass (Da):41,274
Checksum:i8380618C620E266F
GO
Isoform D (identifier: P24350-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-324: Missing.

Note: No experimental confirmation available.

Show »
Length:394
Mass (Da):43,419
Checksum:i069CC77EB0721CD5
GO

Sequence cautioni

The sequence CAA41342.1 differs from that shown. Reason: Frameshift at positions 127 and 216.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 7219Missing in isoform B and isoform C. VSP_018342Add
BLAST
Alternative sequencei132 – 1387Missing in isoform C. VSP_018343
Alternative sequencei323 – 3242Missing in isoform B, isoform C and isoform D. VSP_002342

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661L → V1 Publication
Sequence conflicti216 – 2161A → P1 Publication
Sequence conflicti266 – 2661L → V in CAA41342. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58436 mRNA. Translation: CAA41342.1. Frameshift.
AF086816 mRNA. Translation: AAC35369.1.
AE014298 Genomic DNA. Translation: AAF48447.2.
AE014298 Genomic DNA. Translation: AAN09663.1.
AE014298 Genomic DNA. Translation: AAX52497.1.
AE014298 Genomic DNA. Translation: AAX52498.1.
AH003854 Genomic DNA. Translation: AAB21441.1.
PIRiA42089.
S14795.
RefSeqiNP_001014743.1. NM_001014743.2. [P24350-4]
NP_001014744.1. NM_001014744.1. [P24350-1]
NP_524876.1. NM_080137.2. [P24350-2]
NP_727845.1. NM_167438.1. [P24350-3]
UniGeneiDm.33472.

Genome annotation databases

EnsemblMetazoaiFBtr0074015; FBpp0073832; FBgn0000028. [P24350-2]
GeneIDi47080.
KEGGidme:Dmel_CG9151.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58436 mRNA. Translation: CAA41342.1 . Frameshift.
AF086816 mRNA. Translation: AAC35369.1 .
AE014298 Genomic DNA. Translation: AAF48447.2 .
AE014298 Genomic DNA. Translation: AAN09663.1 .
AE014298 Genomic DNA. Translation: AAX52497.1 .
AE014298 Genomic DNA. Translation: AAX52498.1 .
AH003854 Genomic DNA. Translation: AAB21441.1 .
PIRi A42089.
S14795.
RefSeqi NP_001014743.1. NM_001014743.2. [P24350-4 ]
NP_001014744.1. NM_001014744.1. [P24350-1 ]
NP_524876.1. NM_080137.2. [P24350-2 ]
NP_727845.1. NM_167438.1. [P24350-3 ]
UniGenei Dm.33472.

3D structure databases

ProteinModelPortali P24350.
SMRi P24350. Positions 226-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 70572. 2 interactions.

Proteomic databases

PaxDbi P24350.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0074015 ; FBpp0073832 ; FBgn0000028 . [P24350-2 ]
GeneIDi 47080.
KEGGi dme:Dmel_CG9151.

Organism-specific databases

CTDi 47080.
FlyBasei FBgn0000028. acj6.

Phylogenomic databases

eggNOGi NOG289030.
GeneTreei ENSGT00750000117482.
InParanoidi P24350.
KOi K09366.
OMAi GHSAVHH.
OrthoDBi EOG7RV9G7.
PhylomeDBi P24350.

Miscellaneous databases

ChiTaRSi acj6. drosophila.
GenomeRNAii 47080.
NextBioi 838884.
PROi P24350.

Gene expression databases

Bgeei P24350.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015584. POU4-related.
IPR000327. POU_specific.
[Graphical view ]
PANTHERi PTHR11636:SF10. PTHR11636:SF10. 1 hit.
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "I-POU: a POU-domain protein that inhibits neuron-specific gene activation."
    Treacy M.N., He X., Rosenfeld M.G.
    Nature 350:577-584(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), TISSUE SPECIFICITY.
  2. "The odor specificities of a subset of olfactory receptor neurons are governed by Acj6, a POU-domain transcription factor."
    Clyne P.J., Certel S.J., de Bruyne M., Zaslavsky L., Johnson W.A., Carlson J.R.
    Neuron 22:339-347(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY.
    Strain: Canton-S.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. "Twin of I-POU: a two amino acid difference in the I-POU homeodomain distinguishes an activator from an inhibitor of transcription."
    Treacy M.N., Neilson L.I., Turner E.E., He X., Rosenfeld M.G.
    Cell 68:491-505(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 318-327 (ISOFORMS B/C/D), FUNCTION.
  6. "Similar DNA recognition properties of alternatively spliced Drosophila POU factors."
    Turner E.E.
    Proc. Natl. Acad. Sci. U.S.A. 93:15097-15101(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.

Entry informationi

Entry nameiIPOU_DROME
AccessioniPrimary (citable) accession number: P24350
Secondary accession number(s): O77214
, Q26465, Q59E44, Q59E45, Q8IR36, Q9VXW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 16, 2006
Last modified: July 9, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi