Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24350 (IPOU_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inhibitory POU protein

Short name=I-POU
Alternative name(s):
Abnormal chemosensory jump 6 protein
Gene names
Name:acj6
Synonyms:Ipou
ORF Names:CG9151
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Modulates gene transcription; simultaneously generates both a specific activator and an inhibitor of gene transcription, capable of modulating two distinct regulatory programs during neural development. Has a role in olfactory behavior. Ref.2 Ref.5

Subcellular location

Nucleus.

Tissue specificity

Coexpressed with vvl in overlapping subsets of neurons in the embryonic central nervous system. Expressed in olfactory neurons. Ref.1 Ref.2

Sequence similarities

Belongs to the POU transcription factor family. Class-4 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Caution

Was originally (Ref.1) thought that the I-POU homeobox is unable to bind DNA because it lacks two N-terminal basic residues.

Was originally (Ref.1) thought to interact with vvl.

Sequence caution

The sequence CAA41342.1 differs from that shown. Reason: Frameshift at positions 127 and 216.

Ontologies

Keywords
   Biological processOlfaction
Sensory transduction
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Repressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Inferred from mutant phenotype PubMed 15247920. Source: FlyBase

chemosensory jump behavior

Inferred from mutant phenotype PubMed 2510161. Source: FlyBase

dendrite guidance

Inferred from mutant phenotype PubMed 17276922. Source: FlyBase

dendrite morphogenesis

Inferred from mutant phenotype PubMed 12553905. Source: FlyBase

detection of chemical stimulus involved in sensory perception of smell

Inferred from direct assay PubMed 20371823. Source: FlyBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12097480. Source: FlyBase

synaptic target recognition

Inferred from mutant phenotype PubMed 10804181. Source: FlyBase

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 12097480. Source: FlyBase

transcription corepressor activity

Inferred from genetic interaction Ref.1. Source: FlyBase

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P24350-2)

Also known as: TI-POU; Twin-of-I-POU;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform C (identifier: P24350-1)

Also known as: I-POU;

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     132-138: Missing.
     323-324: Missing.
Isoform B (identifier: P24350-3)

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     323-324: Missing.
Note: No experimental confirmation available.
Isoform D (identifier: P24350-4)

The sequence of this isoform differs from the canonical sequence as follows:
     323-324: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Inhibitory POU protein
PRO_0000100775

Regions

Domain222 – 29978POU-specific
DNA binding320 – 37758Homeobox; atypical Ref.6
Motif86 – 9510POU-IV box

Natural variations

Alternative sequence54 – 7219Missing in isoform B and isoform C.
VSP_018342
Alternative sequence132 – 1387Missing in isoform C.
VSP_018343
Alternative sequence323 – 3242Missing in isoform B, isoform C and isoform D.
VSP_002342

Experimental info

Sequence conflict1661L → V Ref.1
Sequence conflict2161A → P Ref.1
Sequence conflict2661L → V in CAA41342. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform A (TI-POU) (Twin-of-I-POU) [UniParc].

Last modified May 16, 2006. Version 3.
Checksum: AEA80A7EF633B30D

FASTA39643,704
        10         20         30         40         50         60 
MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD SLESRLLEDA 

        70         80         90        100        110        120 
SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA QHVHSQMPSQ IKHDVMYHHH 

       130        140        150        160        170        180 
SMSGPPQRPL QENPFSRQMH HSMDQLDMLD PTGSMTTLAP ISESPLTPTH QHLHGSYHSM 

       190        200        210        220        230        240 
NHMMSHHHPG TLSGHTGGHH GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR 

       250        260        270        280        290        300 
RIKLGVTQAD VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA 

       310        320        330        340        350        360 
QAKNKRRDPD APSVLPAGEK KRKRTSIAAP EKRSLEAYFA VQPRPSGEKI AAIAEKLDLK 

       370        380        390 
KNVVRVWFCN QRQKQKRIVS SVTPSMTGHG SAGFGY 

« Hide

Isoform C (I-POU) [UniParc].

Checksum: 10F6A5623E79F148
Show »

FASTA36840,415
Isoform B [UniParc].

Checksum: 8380618C620E266F
Show »

FASTA37541,274
Isoform D [UniParc].

Checksum: 069CC77EB0721CD5
Show »

FASTA39443,419

References

« Hide 'large scale' references
[1]"I-POU: a POU-domain protein that inhibits neuron-specific gene activation."
Treacy M.N., He X., Rosenfeld M.G.
Nature 350:577-584(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), TISSUE SPECIFICITY.
[2]"The odor specificities of a subset of olfactory receptor neurons are governed by Acj6, a POU-domain transcription factor."
Clyne P.J., Certel S.J., de Bruyne M., Zaslavsky L., Johnson W.A., Carlson J.R.
Neuron 22:339-347(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY.
Strain: Canton-S.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[5]"Twin of I-POU: a two amino acid difference in the I-POU homeodomain distinguishes an activator from an inhibitor of transcription."
Treacy M.N., Neilson L.I., Turner E.E., He X., Rosenfeld M.G.
Cell 68:491-505(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 318-327 (ISOFORMS B/C/D), FUNCTION.
[6]"Similar DNA recognition properties of alternatively spliced Drosophila POU factors."
Turner E.E.
Proc. Natl. Acad. Sci. U.S.A. 93:15097-15101(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58436 mRNA. Translation: CAA41342.1. Frameshift.
AF086816 mRNA. Translation: AAC35369.1.
AE014298 Genomic DNA. Translation: AAF48447.2.
AE014298 Genomic DNA. Translation: AAN09663.1.
AE014298 Genomic DNA. Translation: AAX52497.1.
AE014298 Genomic DNA. Translation: AAX52498.1.
AH003854 Genomic DNA. Translation: AAB21441.1.
PIRA42089.
S14795.
RefSeqNP_001014743.1. NM_001014743.2. [P24350-4]
NP_001014744.1. NM_001014744.1. [P24350-1]
NP_524876.1. NM_080137.2. [P24350-2]
NP_727845.1. NM_167438.1. [P24350-3]
UniGeneDm.33472.

3D structure databases

ProteinModelPortalP24350.
SMRP24350. Positions 226-377.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid70572. 2 interactions.

Proteomic databases

PaxDbP24350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0074015; FBpp0073832; FBgn0000028. [P24350-2]
GeneID47080.
KEGGdme:Dmel_CG9151.

Organism-specific databases

CTD47080.
FlyBaseFBgn0000028. acj6.

Phylogenomic databases

eggNOGNOG289030.
GeneTreeENSGT00750000117482.
InParanoidP24350.
KOK09366.
OMAGHSAVHH.
OrthoDBEOG7RV9G7.
PhylomeDBP24350.

Gene expression databases

BgeeP24350.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015584. POU4-related.
IPR000327. POU_specific.
[Graphical view]
PANTHERPTHR11636:SF10. PTHR11636:SF10. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSacj6. drosophila.
GenomeRNAi47080.
NextBio838884.
PROP24350.

Entry information

Entry nameIPOU_DROME
AccessionPrimary (citable) accession number: P24350
Secondary accession number(s): O77214 expand/collapse secondary AC list , Q26465, Q59E44, Q59E45, Q8IR36, Q9VXW1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 16, 2006
Last modified: July 9, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase