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Protein

Inhibitory POU protein

Gene

acj6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates gene transcription; simultaneously generates both a specific activator and an inhibitor of gene transcription, capable of modulating two distinct regulatory programs during neural development. Has a role in olfactory behavior.2 Publications

Caution

Was originally thought that the I-POU homeobox is unable to bind DNA because it lacks two N-terminal basic residues.1 Publication
Was originally thought to interact with vvl.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi320 – 377Homeobox; atypicalPROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

  • DNA binding transcription factor activity Source: FlyBase
  • sequence-specific DNA binding Source: InterPro
  • transcription corepressor activity Source: FlyBase

GO - Biological processi

  • axon guidance Source: FlyBase
  • chemosensory jump behavior Source: FlyBase
  • dendrite guidance Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • detection of chemical stimulus involved in sensory perception of smell Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • synaptic target recognition Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processOlfaction, Sensory transduction, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory POU protein
Short name:
I-POU
Alternative name(s):
Abnormal chemosensory jump 6 protein
Gene namesi
Name:acj6
Synonyms:Ipou
ORF Names:CG9151
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000028 acj6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007751 – 396Inhibitory POU proteinAdd BLAST396

Proteomic databases

PaxDbiP24350
PRIDEiP24350

Expressioni

Tissue specificityi

Coexpressed with vvl in overlapping subsets of neurons in the embryonic central nervous system. Expressed in olfactory neurons.2 Publications

Gene expression databases

BgeeiFBgn0000028
GenevisibleiP24350 DM

Interactioni

Protein-protein interaction databases

BioGridi70572, 4 interactors
IntActiP24350, 2 interactors
STRINGi7227.FBpp0073832

Structurei

3D structure databases

ProteinModelPortaliP24350
SMRiP24350
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 299POU-specificPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi86 – 95POU-IV box10

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG1168 Eukaryota
ENOG410XPNX LUCA
GeneTreeiENSGT00760000118935
InParanoidiP24350
KOiK09366
OrthoDBiEOG091G0WBK
PhylomeDBiP24350

Family and domain databases

CDDicd00086 homeodomain, 1 hit
cd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P24350-2) [UniParc]FASTAAdd to basket
Also known as: TI-POU, Twin-of-I-POU

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD
60 70 80 90 100
SLESRLLEDA SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA
110 120 130 140 150
QHVHSQMPSQ IKHDVMYHHH SMSGPPQRPL QENPFSRQMH HSMDQLDMLD
160 170 180 190 200
PTGSMTTLAP ISESPLTPTH QHLHGSYHSM NHMMSHHHPG TLSGHTGGHH
210 220 230 240 250
GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR RIKLGVTQAD
260 270 280 290 300
VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA
310 320 330 340 350
QAKNKRRDPD APSVLPAGEK KRKRTSIAAP EKRSLEAYFA VQPRPSGEKI
360 370 380 390
AAIAEKLDLK KNVVRVWFCN QRQKQKRIVS SVTPSMTGHG SAGFGY
Length:396
Mass (Da):43,704
Last modified:May 16, 2006 - v3
Checksum:iAEA80A7EF633B30D
GO
Isoform C (identifier: P24350-1) [UniParc]FASTAAdd to basket
Also known as: I-POU

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     132-138: Missing.
     323-324: Missing.

Show »
Length:368
Mass (Da):40,415
Checksum:i10F6A5623E79F148
GO
Isoform B (identifier: P24350-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     323-324: Missing.

Note: No experimental confirmation available.
Show »
Length:375
Mass (Da):41,274
Checksum:i8380618C620E266F
GO
Isoform D (identifier: P24350-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-324: Missing.

Note: No experimental confirmation available.
Show »
Length:394
Mass (Da):43,419
Checksum:i069CC77EB0721CD5
GO

Sequence cautioni

The sequence CAA41342 differs from that shown. Reason: Frameshift at positions 127 and 216.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166L → V (PubMed:1673230).Curated1
Sequence conflicti216A → P (PubMed:1673230).Curated1
Sequence conflicti266L → V in CAA41342 (PubMed:1673230).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01834254 – 72Missing in isoform B and isoform C. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_018343132 – 138Missing in isoform C. 1 Publication7
Alternative sequenceiVSP_002342323 – 324Missing in isoform B, isoform C and isoform D. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58436 mRNA Translation: CAA41342.1 Frameshift.
AF086816 mRNA Translation: AAC35369.1
AE014298 Genomic DNA Translation: AAF48447.2
AE014298 Genomic DNA Translation: AAN09663.1
AE014298 Genomic DNA Translation: AAX52497.1
AE014298 Genomic DNA Translation: AAX52498.1
AH003854 Genomic DNA Translation: AAB21441.1
PIRiA42089
S14795
RefSeqiNP_001014743.1, NM_001014743.3 [P24350-4]
NP_001014744.1, NM_001014744.2 [P24350-1]
NP_524876.1, NM_080137.3 [P24350-2]
NP_727845.1, NM_167438.2 [P24350-3]
UniGeneiDm.33472

Genome annotation databases

EnsemblMetazoaiFBtr0074015; FBpp0073832; FBgn0000028 [P24350-2]
FBtr0100326; FBpp0099732; FBgn0000028 [P24350-1]
FBtr0100328; FBpp0099734; FBgn0000028 [P24350-4]
FBtr0290063; FBpp0288502; FBgn0000028 [P24350-3]
GeneIDi47080
KEGGidme:Dmel_CG9151

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIPOU_DROME
AccessioniPrimary (citable) accession number: P24350
Secondary accession number(s): O77214
, Q26465, Q59E44, Q59E45, Q8IR36, Q9VXW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 16, 2006
Last modified: March 28, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health