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P24350

- IPOU_DROME

UniProt

P24350 - IPOU_DROME

Protein

Inhibitory POU protein

Gene

acj6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 3 (16 May 2006)
      Previous versions | rss
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    Functioni

    Modulates gene transcription; simultaneously generates both a specific activator and an inhibitor of gene transcription, capable of modulating two distinct regulatory programs during neural development. Has a role in olfactory behavior.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi320 – 37758Homeobox; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: FlyBase
    3. transcription corepressor activity Source: FlyBase

    GO - Biological processi

    1. axon guidance Source: FlyBase
    2. chemosensory jump behavior Source: FlyBase
    3. dendrite guidance Source: FlyBase
    4. dendrite morphogenesis Source: FlyBase
    5. detection of chemical stimulus involved in sensory perception of smell Source: FlyBase
    6. positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
    7. synaptic target recognition Source: FlyBase
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Olfaction, Sensory transduction, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inhibitory POU protein
    Short name:
    I-POU
    Alternative name(s):
    Abnormal chemosensory jump 6 protein
    Gene namesi
    Name:acj6
    Synonyms:Ipou
    ORF Names:CG9151
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0000028. acj6.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 396396Inhibitory POU proteinPRO_0000100775Add
    BLAST

    Proteomic databases

    PaxDbiP24350.

    Expressioni

    Tissue specificityi

    Coexpressed with vvl in overlapping subsets of neurons in the embryonic central nervous system. Expressed in olfactory neurons.2 Publications

    Gene expression databases

    BgeeiP24350.

    Interactioni

    Protein-protein interaction databases

    BioGridi70572. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP24350.
    SMRiP24350. Positions 226-377.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini222 – 29978POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi86 – 9510POU-IV box

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG289030.
    GeneTreeiENSGT00750000117482.
    InParanoidiP24350.
    KOiK09366.
    OMAiGHSAVHH.
    OrthoDBiEOG7RV9G7.
    PhylomeDBiP24350.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015584. POU4-related.
    IPR000327. POU_specific.
    [Graphical view]
    PANTHERiPTHR11636:SF10. PTHR11636:SF10. 1 hit.
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P24350-2) [UniParc]FASTAAdd to Basket

    Also known as: TI-POU, Twin-of-I-POU

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD    50
    SLESRLLEDA SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA 100
    QHVHSQMPSQ IKHDVMYHHH SMSGPPQRPL QENPFSRQMH HSMDQLDMLD 150
    PTGSMTTLAP ISESPLTPTH QHLHGSYHSM NHMMSHHHPG TLSGHTGGHH 200
    GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR RIKLGVTQAD 250
    VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA 300
    QAKNKRRDPD APSVLPAGEK KRKRTSIAAP EKRSLEAYFA VQPRPSGEKI 350
    AAIAEKLDLK KNVVRVWFCN QRQKQKRIVS SVTPSMTGHG SAGFGY 396
    Length:396
    Mass (Da):43,704
    Last modified:May 16, 2006 - v3
    Checksum:iAEA80A7EF633B30D
    GO
    Isoform C (identifier: P24350-1) [UniParc]FASTAAdd to Basket

    Also known as: I-POU

    The sequence of this isoform differs from the canonical sequence as follows:
         54-72: Missing.
         132-138: Missing.
         323-324: Missing.

    Show »
    Length:368
    Mass (Da):40,415
    Checksum:i10F6A5623E79F148
    GO
    Isoform B (identifier: P24350-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         54-72: Missing.
         323-324: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:375
    Mass (Da):41,274
    Checksum:i8380618C620E266F
    GO
    Isoform D (identifier: P24350-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         323-324: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:394
    Mass (Da):43,419
    Checksum:i069CC77EB0721CD5
    GO

    Sequence cautioni

    The sequence CAA41342.1 differs from that shown. Reason: Frameshift at positions 127 and 216.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti166 – 1661L → V(PubMed:1673230)Curated
    Sequence conflicti216 – 2161A → P(PubMed:1673230)Curated
    Sequence conflicti266 – 2661L → V in CAA41342. (PubMed:1673230)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei54 – 7219Missing in isoform B and isoform C. 1 PublicationVSP_018342Add
    BLAST
    Alternative sequencei132 – 1387Missing in isoform C. 1 PublicationVSP_018343
    Alternative sequencei323 – 3242Missing in isoform B, isoform C and isoform D. 1 PublicationVSP_002342

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58436 mRNA. Translation: CAA41342.1. Frameshift.
    AF086816 mRNA. Translation: AAC35369.1.
    AE014298 Genomic DNA. Translation: AAF48447.2.
    AE014298 Genomic DNA. Translation: AAN09663.1.
    AE014298 Genomic DNA. Translation: AAX52497.1.
    AE014298 Genomic DNA. Translation: AAX52498.1.
    AH003854 Genomic DNA. Translation: AAB21441.1.
    PIRiA42089.
    S14795.
    RefSeqiNP_001014743.1. NM_001014743.2. [P24350-4]
    NP_001014744.1. NM_001014744.1. [P24350-1]
    NP_524876.1. NM_080137.2. [P24350-2]
    NP_727845.1. NM_167438.1. [P24350-3]
    UniGeneiDm.33472.

    Genome annotation databases

    EnsemblMetazoaiFBtr0074015; FBpp0073832; FBgn0000028. [P24350-2]
    GeneIDi47080.
    KEGGidme:Dmel_CG9151.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58436 mRNA. Translation: CAA41342.1 . Frameshift.
    AF086816 mRNA. Translation: AAC35369.1 .
    AE014298 Genomic DNA. Translation: AAF48447.2 .
    AE014298 Genomic DNA. Translation: AAN09663.1 .
    AE014298 Genomic DNA. Translation: AAX52497.1 .
    AE014298 Genomic DNA. Translation: AAX52498.1 .
    AH003854 Genomic DNA. Translation: AAB21441.1 .
    PIRi A42089.
    S14795.
    RefSeqi NP_001014743.1. NM_001014743.2. [P24350-4 ]
    NP_001014744.1. NM_001014744.1. [P24350-1 ]
    NP_524876.1. NM_080137.2. [P24350-2 ]
    NP_727845.1. NM_167438.1. [P24350-3 ]
    UniGenei Dm.33472.

    3D structure databases

    ProteinModelPortali P24350.
    SMRi P24350. Positions 226-377.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 70572. 2 interactions.

    Proteomic databases

    PaxDbi P24350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0074015 ; FBpp0073832 ; FBgn0000028 . [P24350-2 ]
    GeneIDi 47080.
    KEGGi dme:Dmel_CG9151.

    Organism-specific databases

    CTDi 47080.
    FlyBasei FBgn0000028. acj6.

    Phylogenomic databases

    eggNOGi NOG289030.
    GeneTreei ENSGT00750000117482.
    InParanoidi P24350.
    KOi K09366.
    OMAi GHSAVHH.
    OrthoDBi EOG7RV9G7.
    PhylomeDBi P24350.

    Miscellaneous databases

    ChiTaRSi acj6. drosophila.
    GenomeRNAii 47080.
    NextBioi 838884.
    PROi P24350.

    Gene expression databases

    Bgeei P24350.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015584. POU4-related.
    IPR000327. POU_specific.
    [Graphical view ]
    PANTHERi PTHR11636:SF10. PTHR11636:SF10. 1 hit.
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "I-POU: a POU-domain protein that inhibits neuron-specific gene activation."
      Treacy M.N., He X., Rosenfeld M.G.
      Nature 350:577-584(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), TISSUE SPECIFICITY.
    2. "The odor specificities of a subset of olfactory receptor neurons are governed by Acj6, a POU-domain transcription factor."
      Clyne P.J., Certel S.J., de Bruyne M., Zaslavsky L., Johnson W.A., Carlson J.R.
      Neuron 22:339-347(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY.
      Strain: Canton-S.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    5. "Twin of I-POU: a two amino acid difference in the I-POU homeodomain distinguishes an activator from an inhibitor of transcription."
      Treacy M.N., Neilson L.I., Turner E.E., He X., Rosenfeld M.G.
      Cell 68:491-505(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 318-327 (ISOFORMS B/C/D), FUNCTION.
    6. "Similar DNA recognition properties of alternatively spliced Drosophila POU factors."
      Turner E.E.
      Proc. Natl. Acad. Sci. U.S.A. 93:15097-15101(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.

    Entry informationi

    Entry nameiIPOU_DROME
    AccessioniPrimary (citable) accession number: P24350
    Secondary accession number(s): O77214
    , Q26465, Q59E44, Q59E45, Q8IR36, Q9VXW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 139 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3