P24350 (IPOU_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inhibitory POU protein
Short name=I-POU

Alternative name(s):
Abnormal chemosensory jump 6 protein

Gene names

Name:	acj6
Synonyms:	Ipou
ORF Names:	CG9151

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulates gene transcription; simultaneously generates both a specific activator and inhibitor of gene transcription, capable of modulating two distinct regulatory programs during neural development. Has a role in olfactory behavior. Ref.2 Ref.5
Subcellular location	Nucleus.
Tissue specificity	Coexpressed with acj6 in overlapping subsets of neurons in the embryonic central nervous system. Expressed in olfactory neurons. Ref.1 Ref.2
Sequence similarities	Belongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
Caution	Was originally (Ref.1) thought that the I-POU homeobox is unable to bind DNA because it lacks two N-terminal basic residues. Was originally (Ref.1) thought to interact with vvl.
Sequence caution	The sequence CAA41342.1 differs from that shown. Reason: Frameshift at positions 127 and 216.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axon guidance Inferred from mutant phenotype. Source: FlyBase chemosensory jump behavior Inferred from mutant phenotype. Source: FlyBase dendrite guidance Inferred from mutant phenotype. Source: FlyBase detection of chemical stimulus involved in sensory perception of smell Inferred from direct assay. Source: FlyBase synaptic target recognition Inferred from mutant phenotype. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription corepressor activity Inferred from physical interaction Ref.1. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P24350-2)

Also known as: TI-POU; Twin-of-I-POU;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C (identifier: P24350-1)

Also known as: I-POU;

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     132-138: Missing.
     323-324: Missing.

Isoform B (identifier: P24350-3)

The sequence of this isoform differs from the canonical sequence as follows:
     54-72: Missing.
     323-324: Missing.

Note: No experimental confirmation available.

Isoform D (identifier: P24350-4)

The sequence of this isoform differs from the canonical sequence as follows:
     323-324: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Inhibitory POU protein		PRO_0000100775
Regions
	Domain	222 – 299	78	POU-specific
	DNA binding	320 – 377	58	Homeobox; atypical Ref.6
	Motif	86 – 95	10	POU-IV box
Natural variations
	Alternative sequence	54 – 72	19	Missing in isoform B and isoform C.		VSP_018342
	Alternative sequence	132 – 138	7	Missing in isoform C.		VSP_018343
	Alternative sequence	323 – 324	2	Missing in isoform B, isoform C and isoform D.		VSP_002342
Experimental info
	Sequence conflict	166	1	L → V Ref.1
	Sequence conflict	216	1	A → P Ref.1
	Sequence conflict	266	1	L → V in CAA41342. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (TI-POU) (Twin-of-I-POU) [UniParc]. Last modified May 16, 2006. Version 3. Checksum: AEA80A7EF633B30D	FASTA	396	43,704
	10 20 30 40 50 60 MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD SLESRLLEDA 70 80 90 100 110 120 SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA QHVHSQMPSQ IKHDVMYHHH 130 140 150 160 170 180 SMSGPPQRPL QENPFSRQMH HSMDQLDMLD PTGSMTTLAP ISESPLTPTH QHLHGSYHSM 190 200 210 220 230 240 NHMMSHHHPG TLSGHTGGHH GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR 250 260 270 280 290 300 RIKLGVTQAD VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA 310 320 330 340 350 360 QAKNKRRDPD APSVLPAGEK KRKRTSIAAP EKRSLEAYFA VQPRPSGEKI AAIAEKLDLK 370 380 390 KNVVRVWFCN QRQKQKRIVS SVTPSMTGHG SAGFGY
	Isoform C (I-POU) [UniParc]. Checksum: 10F6A5623E79F148	FASTA	368	40,415
	10 20 30 40 50 60 MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD SLEGDIFAGI 70 80 90 100 110 120 NDGILSRAEA LAAVDIQKHQ AQHVHSQMPS QIKHDVMYHH HSMSGPPQRP LQMHHSMDQL 130 140 150 160 170 180 DMLDPTGSMT TLAPISESPL TPTHQHLHGS YHSMNHMMSH HHPGTLSGHT GGHHGHSAVH 190 200 210 220 230 240 HPVITAAVAA AGLHPDTDTD PRELEAFAER FKQRRIKLGV TQADVGKALA NLKLPGVGAL 250 260 270 280 290 300 SQSTICRFES LTLSHNNMIA LKPILQAWLE EAEAQAKNKR RDPDAPSVLP AGEKKRTSIA 310 320 330 340 350 360 APEKRSLEAY FAVQPRPSGE KIAAIAEKLD LKKNVVRVWF CNQRQKQKRI VSSVTPSMTG HGSAGFGY
	Isoform B [UniParc]. Checksum: 8380618C620E266F	FASTA	375	41,274
	10 20 30 40 50 60 MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD SLEGDIFAGI 70 80 90 100 110 120 NDGILSRAEA LAAVDIQKHQ AQHVHSQMPS QIKHDVMYHH HSMSGPPQRP LQENPFSRQM 130 140 150 160 170 180 HHSMDQLDML DPTGSMTTLA PISESPLTPT HQHLHGSYHS MNHMMSHHHP GTLSGHTGGH 190 200 210 220 230 240 HGHSAVHHPV ITAAVAAAGL HPDTDTDPRE LEAFAERFKQ RRIKLGVTQA DVGKALANLK 250 260 270 280 290 300 LPGVGALSQS TICRFESLTL SHNNMIALKP ILQAWLEEAE AQAKNKRRDP DAPSVLPAGE 310 320 330 340 350 360 KKRTSIAAPE KRSLEAYFAV QPRPSGEKIA AIAEKLDLKK NVVRVWFCNQ RQKQKRIVSS 370 VTPSMTGHGS AGFGY
	Isoform D [UniParc]. Checksum: 069CC77EB0721CD5	FASTA	394	43,419
	10 20 30 40 50 60 MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD SLESRLLEDA 70 80 90 100 110 120 SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA QHVHSQMPSQ IKHDVMYHHH 130 140 150 160 170 180 SMSGPPQRPL QENPFSRQMH HSMDQLDMLD PTGSMTTLAP ISESPLTPTH QHLHGSYHSM 190 200 210 220 230 240 NHMMSHHHPG TLSGHTGGHH GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR 250 260 270 280 290 300 RIKLGVTQAD VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA 310 320 330 340 350 360 QAKNKRRDPD APSVLPAGEK KRTSIAAPEK RSLEAYFAVQ PRPSGEKIAA IAEKLDLKKN 370 380 390 VVRVWFCNQR QKQKRIVSSV TPSMTGHGSA GFGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"I-POU: a POU-domain protein that inhibits neuron-specific gene activation." Treacy M.N., He X., Rosenfeld M.G. Nature 350:577-584(1991) [PubMed: 1673230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), TISSUE SPECIFICITY.
[2]	"The odor specificities of a subset of olfactory receptor neurons are governed by Acj6, a POU-domain transcription factor." Clyne P.J., Certel S.J., de Bruyne M., Zaslavsky L., Johnson W.A., Carlson J.R. Neuron 22:339-347(1999) [PubMed: 10069339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"Twin of I-POU: a two amino acid difference in the I-POU homeodomain distinguishes an activator from an inhibitor of transcription." Treacy M.N., Neilson L.I., Turner E.E., He X., Rosenfeld M.G. Cell 68:491-505(1992) [PubMed: 1346754] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 318-327 (ISOFORMS B/C/D), FUNCTION.
[6]	"Similar DNA recognition properties of alternatively spliced Drosophila POU factors." Turner E.E. Proc. Natl. Acad. Sci. U.S.A. 93:15097-15101(1996) [PubMed: 8986770] [Abstract] Cited for: DNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58436 mRNA. Translation: CAA41342.1. Frameshift. AF086816 mRNA. Translation: AAC35369.1. AE014298 Genomic DNA. Translation: AAF48447.2. AE014298 Genomic DNA. Translation: AAN09663.1. AE014298 Genomic DNA. Translation: AAX52497.1. AE014298 Genomic DNA. Translation: AAX52498.1. S82271, S82267 Genomic DNA. Translation: AAB21441.1.
PIR	A42089. S14795.
RefSeq	NP_001014743.1. NM_001014743.1. NP_001014744.1. NM_001014744.1. NP_524876.1. NM_080137.2. NP_727845.1. NM_167438.1.
UniGene	Dm.33472.
3D structure databases
ProteinModelPortal	P24350.
SMR	P24350. Positions 222-379.
ModBase	Search...
Protein-protein interaction databases
STRING	P24350.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074015; FBpp0073832; FBgn0000028.
GeneID	47080.
KEGG	dme:Dmel_CG9151.
NMPDR	fig|7227.3.peg.17925.
Organism-specific databases
CTD	47080.
FlyBase	FBgn0000028. acj6.
Phylogenomic databases
eggNOG	inNOG09026.
GeneTree	EMGT00050000001591.
InParanoid	P24350.
OMA	GHSAVHH.
OrthoDB	EOG4RBP15.
PhylomeDB	P24350.
Gene expression databases
Bgee	P24350.
GermOnline	CG9151. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR010982. Lambda_DNA-bd. IPR013847. POU. IPR000327. POU_specific. [Graphical view]
Gene3D	G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit. G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K09366.
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PRINTS	PR00028. POUDOMAIN.
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	838884.

Entry information

Entry name

IPOU_DROME

Accession

Primary (citable) accession number: P24350
Secondary accession number(s): O77214 , Q26465, Q59E44, Q59E45, Q8IR36, Q9VXW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project