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P24348

- LET23_CAEEL

UniProt

P24348 - LET23_CAEEL

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Protein

Receptor tyrosine-protein kinase let-23

Gene

let-23

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Tyrosine kinase receptor required for the induction of vulval differentiation. Possible receptor for the inductive signal required for vulval development. Activated by lin-3 and acts by way of let-60 RAS. The lin-3/let-23 pair is a simplified version of the mammalian neuregulin-ERBB network.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei919 – 9191ATPPROSITE-ProRule annotation
Active sitei1010 – 10101Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi891 – 8999ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. epidermal growth factor-activated receptor activity Source: WormBase

GO - Biological processi

  1. epidermal growth factor receptor signaling pathway Source: WormBase
  2. male genitalia development Source: WormBase
  3. nematode larval development Source: WormBase
  4. ovulation Source: WormBase
  5. positive regulation of vulval development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1045.
ReactomeiREACT_182985. EGFR Transactivation by Gastrin.
REACT_184110. Signaling by ERBB2.
REACT_220181. Sema4D induced cell migration and growth-cone collapse.
REACT_236360. Signal transduction by L1.
REACT_243535. Downregulation of ERBB4 signaling.
REACT_250031. Signaling by constitutively active EGFR.
REACT_258320. EGFR downregulation.
REACT_258387. GRB2 events in EGFR signaling.
REACT_261452. SHC1 events in EGFR signaling.
REACT_262378. Nuclear signaling by ERBB4.
SignaLinkiP24348.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor tyrosine-protein kinase let-23 (EC:2.7.10.1)
Alternative name(s):
Lethal protein 23
Gene namesi
Name:let-23
Synonyms:kin-7
ORF Names:ZK1067.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiZK1067.1a; CE03840; WBGene00002299; let-23.

Subcellular locationi

Apical cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Basolateral cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Note: Basolateral and apical membrane of cell junctions in epithelial vulval precursor cells.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 818798ExtracellularSequence AnalysisAdd
BLAST
Transmembranei819 – 83921HelicalSequence AnalysisAdd
BLAST
Topological domaini840 – 1323484CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: WormBase
  2. basolateral plasma membrane Source: WormBase
  3. cell-cell junction Source: WormBase
  4. integral component of membrane Source: UniProtKB-KW
  5. lateral plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi368 – 3681C → Y in SY10. 1 Publication
Mutagenesisi469 – 4691G → R in MN216. 1 Publication
Mutagenesisi700 – 7001C → W in MN23. 1 Publication
Mutagenesisi753 – 7531C → Y in SY11. 1 Publication
Mutagenesisi1065 – 10651T → I in SY16. 1 Publication
Mutagenesisi1074 – 10741G → E in SY7. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 13231303Receptor tyrosine-protein kinase let-23PRO_0000016677Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi220 ↔ 228By similarity
Disulfide bondi224 ↔ 236By similarity
Disulfide bondi244 ↔ 251By similarity
Disulfide bondi248 ↔ 262By similarity
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi263 ↔ 271By similarity
Disulfide bondi267 ↔ 279By similarity
Disulfide bondi282 ↔ 291By similarity
Disulfide bondi295 ↔ 322By similarity
Disulfide bondi326 ↔ 337By similarity
Disulfide bondi341 ↔ 356By similarity
Disulfide bondi359 ↔ 364By similarity
Glycosylationi376 – 3761N-linked (GlcNAc...)1 Publication
Disulfide bondi520 ↔ 529By similarity
Disulfide bondi524 ↔ 537By similarity
Disulfide bondi540 ↔ 549By similarity
Disulfide bondi553 ↔ 567By similarity
Glycosylationi561 – 5611N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi570 ↔ 577By similarity
Disulfide bondi574 ↔ 585By similarity
Disulfide bondi588 ↔ 604By similarity
Disulfide bondi608 ↔ 620By similarity
Disulfide bondi623 ↔ 632By similarity
Disulfide bondi627 ↔ 644By similarity
Disulfide bondi647 ↔ 660Sequence Analysis
Glycosylationi655 – 6551N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi670 ↔ 693Sequence Analysis
Disulfide bondi696 ↔ 703Sequence Analysis
Disulfide bondi700 ↔ 715Sequence Analysis
Disulfide bondi717 ↔ 731Sequence Analysis
Disulfide bondi735 ↔ 750Sequence Analysis
Glycosylationi746 – 7461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi753 ↔ 763Sequence Analysis
Disulfide bondi757 ↔ 771Sequence Analysis
Disulfide bondi774 ↔ 787Sequence Analysis
Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi791 ↔ 805Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP24348.

Expressioni

Tissue specificityi

Vulval precursor cells.

Developmental stagei

Expressed during L2 and L3 larval stages.

Gene expression databases

ExpressionAtlasiP24348. baseline.

Interactioni

Protein-protein interaction databases

BioGridi39787. 107 interactions.
DIPiDIP-1014N.
IntActiP24348. 2 interactions.
MINTiMINT-1515688.

Structurei

3D structure databases

ProteinModelPortaliP24348.
SMRiP24348. Positions 7-644, 861-1156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini885 – 1152268Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118799.
HOGENOMiHOG000016236.
InParanoidiP24348.
KOiK04361.
PhylomeDBiP24348.

Family and domain databases

Gene3Di3.80.20.20. 2 hits.
InterProiIPR000494. EGF_rcpt_L.
IPR006211. Furin-like_Cys-rich_dom.
IPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00757. Furin-like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF01030. Recep_L_domain. 2 hits.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00261. FU. 7 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24348-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRYPPSIGSI LLIIPIFLTF FGNSNAQLWK RCVSPQDCLC SGTTNGISRY
60 70 80 90 100
GTGNILEDLE TMYRGCRRVY GNLEITWIEA NEIKKWREST NSTVDPKNED
110 120 130 140 150
SPLKSINFFD NLEEIRGSLI IYRANIQKIS FPRLRVIYGD EVFHDNALYI
160 170 180 190 200
HKNDKVHEVV MRELRVIRNG SVTIQDNPKM CYIGDKIDWK ELLYDPDVQK
210 220 230 240 250
VETTNSHQHC YQNGKSMAKC HESCNDKCWG SGDNDCQRVY RSVCPKSCSQ
260 270 280 290 300
CFYSNSTSSY ECCDSACLGG CTGHGPKNCI ACSKYELDGI CIETCPSRKI
310 320 330 340 350
FNHKTGRLVF NPDGRYQNGN HCVKECPPEL LIENDVCVRH CSDGHHYDAT
360 370 380 390 400
KDVRECEKCR SSSCPKICTV DGHLTNETLK NLEGCEQIDG HLIIEHAFTY
410 420 430 440 450
EQLKVLETVK IVSEYITIVQ QNFYDLKFLK NLQIIEGRKL HNVRWALAIY
460 470 480 490 500
QCDDLEELSL NSLKLIKTGA VLIMKNHRLC YVSKIDWSSI ITSKGKDNKP
510 520 530 540 550
SLAIAENRDS KLCETEQRVC DKNCNKRGCW GKEPEDCLEC KTWKSVGTCV
560 570 580 590 600
EKCDTKGFLR NQTSMKCERC SPECETCNGL GELDCLTCRH KTLYNSDFGN
610 620 630 640 650
RMECVHDCPV SHFPTQKNVC EKCHPTCYDN GCTGPDSNLG YGGCKQCKYA
660 670 680 690 700
VKYENDTIFC LQSSGMNNVC VENDLPNYYI STYDTEGVIE THCEKCSISC
710 720 730 740 750
KTCSSAGRNV VQNKCVCKHV EYQPNPSERI CMDQCPVNSF MVPDTNNTVC
760 770 780 790 800
KKCHHECDQN YHCANGQSTG CQKCKNFTVF KGDIAQCVSE CPKNLPFSNP
810 820 830 840 850
ANGECLDYDI ASRQRKTRMV IIGSVLFGFA VMFLFILLVY WRCQRIGKKL
860 870 880 890 900
KIAEMVDMPE LTPIDASVRP NMSRICLIPS SELQTKLDKK LGAGAFGTVF
910 920 930 940 950
AGIYYPKRAK NVKIPVAIKV FQTDQSQTDE MLEEATNMFR LRHDNLLKII
960 970 980 990 1000
GFCMHDDGLK IVTIYRPLGN LQNFLKLHKE NLGAREQVLY CYQIASGMQY
1010 1020 1030 1040 1050
LEKQRVVHRD LATRNVLVKK FNHVEITDFG LSKILKHDAD SITIKSGKVA
1060 1070 1080 1090 1100
IKWLAIEIFS KHCYTHASDV WAFGVTCWEI ITFGQSPYQG MSTDSIHNFL
1110 1120 1130 1140 1150
KDGNRLSQPP NCSQDLYQEL LRCWMADPKS RPGFEILYER FKEFCKVPQL
1160 1170 1180 1190 1200
FLENSNKISE SDLSAEERFQ TERIREMFDG NIDPQMYFDQ GSLPSMPSSP
1210 1220 1230 1240 1250
TSMATFTIPH GDLMNRMQSV NSSRYKTEPF DYGSTAQEDN SYLIPKTKEV
1260 1270 1280 1290 1300
QQSAVLYTAV TNEDGQTELS PSNGDYYNQP NTPSSSSGYY NEPHLKTKKP
1310 1320
ETSEEAEAVQ YENEEVSQKE TCL
Length:1,323
Mass (Da):150,511
Last modified:July 25, 2006 - v3
Checksum:i6B0307EE53EEFA99
GO

Sequence cautioni

The sequence BAA09729.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1179 – 11791D → H in CAA40919. (PubMed:1979659)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57767 mRNA. Translation: CAA40919.1.
D63426 Genomic DNA. Translation: BAA09729.1. Sequence problems.
Z70038 Genomic DNA. Translation: CAA93882.3.
PIRiE88257.
S70712.
RefSeqiNP_495962.2. NM_063561.4.
UniGeneiCel.16858.

Genome annotation databases

EnsemblMetazoaiZK1067.1a; ZK1067.1a; WBGene00002299.
GeneIDi174462.
KEGGicel:CELE_ZK1067.1.
UCSCiZK1067.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57767 mRNA. Translation: CAA40919.1 .
D63426 Genomic DNA. Translation: BAA09729.1 . Sequence problems.
Z70038 Genomic DNA. Translation: CAA93882.3 .
PIRi E88257.
S70712.
RefSeqi NP_495962.2. NM_063561.4.
UniGenei Cel.16858.

3D structure databases

ProteinModelPortali P24348.
SMRi P24348. Positions 7-644, 861-1156.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 39787. 107 interactions.
DIPi DIP-1014N.
IntActi P24348. 2 interactions.
MINTi MINT-1515688.

Proteomic databases

PaxDbi P24348.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai ZK1067.1a ; ZK1067.1a ; WBGene00002299 .
GeneIDi 174462.
KEGGi cel:CELE_ZK1067.1.
UCSCi ZK1067.1. c. elegans.

Organism-specific databases

CTDi 174462.
WormBasei ZK1067.1a ; CE03840 ; WBGene00002299 ; let-23.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118799.
HOGENOMi HOG000016236.
InParanoidi P24348.
KOi K04361.
PhylomeDBi P24348.

Enzyme and pathway databases

BRENDAi 2.7.10.1. 1045.
Reactomei REACT_182985. EGFR Transactivation by Gastrin.
REACT_184110. Signaling by ERBB2.
REACT_220181. Sema4D induced cell migration and growth-cone collapse.
REACT_236360. Signal transduction by L1.
REACT_243535. Downregulation of ERBB4 signaling.
REACT_250031. Signaling by constitutively active EGFR.
REACT_258320. EGFR downregulation.
REACT_258387. GRB2 events in EGFR signaling.
REACT_261452. SHC1 events in EGFR signaling.
REACT_262378. Nuclear signaling by ERBB4.
SignaLinki P24348.

Miscellaneous databases

NextBioi 884138.

Gene expression databases

ExpressionAtlasi P24348. baseline.

Family and domain databases

Gene3Di 3.80.20.20. 2 hits.
InterProi IPR000494. EGF_rcpt_L.
IPR006211. Furin-like_Cys-rich_dom.
IPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF00757. Furin-like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF01030. Recep_L_domain. 2 hits.
[Graphical view ]
PRINTSi PR00109. TYRKINASE.
SMARTi SM00261. FU. 7 hits.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 3 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor subfamily."
    Aroian R.V., Koga M., Mendel J.E., Ohshima Y., Sternberg P.W.
    Nature 348:693-699(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic structure and 5' regulatory regions of the let-23 gene in the nematode C. elegans."
    Sakai T., Koga M., Ohshima Y.
    J. Mol. Biol. 256:548-555(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "Mutations in the Caenorhabditis elegans let-23 EGFR-like gene define elements important for cell-type specificity and function."
    Aroian R.V., Les G.M., Sternberg P.W.
    EMBO J. 13:360-366(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-368; GLY-469; CYS-700; CYS-753; THR-1065 AND GLY-1074.
  5. "Basolateral localization of the Caenorhabditis elegans epidermal growth factor receptor in epithelial cells by the PDZ protein lin-10."
    Whitfield C.W., Benard C., Barnes T., Hekimi S., Kim S.K.
    Mol. Biol. Cell 10:2087-2100(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: Bristol N2.
  6. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-376, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiLET23_CAEEL
AccessioniPrimary (citable) accession number: P24348
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 25, 2006
Last modified: November 26, 2014
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3