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P24348

- LET23_CAEEL

UniProt

P24348 - LET23_CAEEL

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Protein

Receptor tyrosine-protein kinase let-23

Gene
let-23, kin-7, ZK1067.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Tyrosine kinase receptor required for the induction of vulval differentiation. Possible receptor for the inductive signal required for vulval development. Activated by lin-3 and acts by way of let-60 RAS. The lin-3/let-23 pair is a simplified version of the mammalian neuregulin-ERBB network.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei919 – 9191ATP By similarity
Active sitei1010 – 10101Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi891 – 8999ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. epidermal growth factor-activated receptor activity Source: WormBase

GO - Biological processi

  1. epidermal growth factor receptor signaling pathway Source: WormBase
  2. male genitalia development Source: WormBase
  3. nematode larval development Source: WormBase
  4. ovulation Source: WormBase
  5. positive regulation of vulval development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1045.
SignaLinkiP24348.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor tyrosine-protein kinase let-23 (EC:2.7.10.1)
Alternative name(s):
Lethal protein 23
Gene namesi
Name:let-23
Synonyms:kin-7
ORF Names:ZK1067.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiZK1067.1a; CE03840; WBGene00002299; let-23.

Subcellular locationi

Apical cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein
Note: Basolateral and apical membrane of cell junctions in epithelial vulval precursor cells.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 818798Extracellular Reviewed predictionAdd
BLAST
Transmembranei819 – 83921Helical; Reviewed predictionAdd
BLAST
Topological domaini840 – 1323484Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: WormBase
  2. basolateral plasma membrane Source: WormBase
  3. cell-cell junction Source: WormBase
  4. integral component of membrane Source: UniProtKB-KW
  5. lateral plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi368 – 3681C → Y in SY10. 1 Publication
Mutagenesisi469 – 4691G → R in MN216. 1 Publication
Mutagenesisi700 – 7001C → W in MN23. 1 Publication
Mutagenesisi753 – 7531C → Y in SY11. 1 Publication
Mutagenesisi1065 – 10651T → I in SY16. 1 Publication
Mutagenesisi1074 – 10741G → E in SY7. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 13231303Receptor tyrosine-protein kinase let-23PRO_0000016677Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi169 – 1691N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi220 ↔ 228 By similarity
Disulfide bondi224 ↔ 236 By similarity
Disulfide bondi244 ↔ 251 By similarity
Disulfide bondi248 ↔ 262 By similarity
Glycosylationi255 – 2551N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi263 ↔ 271 By similarity
Disulfide bondi267 ↔ 279 By similarity
Disulfide bondi282 ↔ 291 By similarity
Disulfide bondi295 ↔ 322 By similarity
Disulfide bondi326 ↔ 337 By similarity
Disulfide bondi341 ↔ 356 By similarity
Disulfide bondi359 ↔ 364 By similarity
Glycosylationi376 – 3761N-linked (GlcNAc...)1 Publication
Disulfide bondi520 ↔ 529 By similarity
Disulfide bondi524 ↔ 537 By similarity
Disulfide bondi540 ↔ 549 By similarity
Disulfide bondi553 ↔ 567 By similarity
Glycosylationi561 – 5611N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi570 ↔ 577 By similarity
Disulfide bondi574 ↔ 585 By similarity
Disulfide bondi588 ↔ 604 By similarity
Disulfide bondi608 ↔ 620 By similarity
Disulfide bondi623 ↔ 632 By similarity
Disulfide bondi627 ↔ 644 By similarity
Disulfide bondi647 ↔ 660 Reviewed prediction
Glycosylationi655 – 6551N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi670 ↔ 693 Reviewed prediction
Disulfide bondi696 ↔ 703 Reviewed prediction
Disulfide bondi700 ↔ 715 Reviewed prediction
Disulfide bondi717 ↔ 731 Reviewed prediction
Disulfide bondi735 ↔ 750 Reviewed prediction
Glycosylationi746 – 7461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi753 ↔ 763 Reviewed prediction
Disulfide bondi757 ↔ 771 Reviewed prediction
Disulfide bondi774 ↔ 787 Reviewed prediction
Glycosylationi776 – 7761N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi791 ↔ 805 Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP24348.

Expressioni

Tissue specificityi

Vulval precursor cells.

Developmental stagei

Expressed during L2 and L3 larval stages.

Interactioni

Protein-protein interaction databases

BioGridi39787. 107 interactions.
DIPiDIP-1014N.
IntActiP24348. 2 interactions.
MINTiMINT-1515688.

Structurei

3D structure databases

ProteinModelPortaliP24348.
SMRiP24348. Positions 7-644, 860-1181.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini885 – 1152268Protein kinaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000115459.
HOGENOMiHOG000016236.
KOiK04361.
PhylomeDBiP24348.

Family and domain databases

Gene3Di3.80.20.20. 2 hits.
InterProiIPR000494. EGF_rcpt_L.
IPR006211. Furin-like_Cys-rich_dom.
IPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00757. Furin-like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF01030. Recep_L_domain. 2 hits.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00261. FU. 7 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24348-1 [UniParc]FASTAAdd to Basket

« Hide

MRYPPSIGSI LLIIPIFLTF FGNSNAQLWK RCVSPQDCLC SGTTNGISRY     50
GTGNILEDLE TMYRGCRRVY GNLEITWIEA NEIKKWREST NSTVDPKNED 100
SPLKSINFFD NLEEIRGSLI IYRANIQKIS FPRLRVIYGD EVFHDNALYI 150
HKNDKVHEVV MRELRVIRNG SVTIQDNPKM CYIGDKIDWK ELLYDPDVQK 200
VETTNSHQHC YQNGKSMAKC HESCNDKCWG SGDNDCQRVY RSVCPKSCSQ 250
CFYSNSTSSY ECCDSACLGG CTGHGPKNCI ACSKYELDGI CIETCPSRKI 300
FNHKTGRLVF NPDGRYQNGN HCVKECPPEL LIENDVCVRH CSDGHHYDAT 350
KDVRECEKCR SSSCPKICTV DGHLTNETLK NLEGCEQIDG HLIIEHAFTY 400
EQLKVLETVK IVSEYITIVQ QNFYDLKFLK NLQIIEGRKL HNVRWALAIY 450
QCDDLEELSL NSLKLIKTGA VLIMKNHRLC YVSKIDWSSI ITSKGKDNKP 500
SLAIAENRDS KLCETEQRVC DKNCNKRGCW GKEPEDCLEC KTWKSVGTCV 550
EKCDTKGFLR NQTSMKCERC SPECETCNGL GELDCLTCRH KTLYNSDFGN 600
RMECVHDCPV SHFPTQKNVC EKCHPTCYDN GCTGPDSNLG YGGCKQCKYA 650
VKYENDTIFC LQSSGMNNVC VENDLPNYYI STYDTEGVIE THCEKCSISC 700
KTCSSAGRNV VQNKCVCKHV EYQPNPSERI CMDQCPVNSF MVPDTNNTVC 750
KKCHHECDQN YHCANGQSTG CQKCKNFTVF KGDIAQCVSE CPKNLPFSNP 800
ANGECLDYDI ASRQRKTRMV IIGSVLFGFA VMFLFILLVY WRCQRIGKKL 850
KIAEMVDMPE LTPIDASVRP NMSRICLIPS SELQTKLDKK LGAGAFGTVF 900
AGIYYPKRAK NVKIPVAIKV FQTDQSQTDE MLEEATNMFR LRHDNLLKII 950
GFCMHDDGLK IVTIYRPLGN LQNFLKLHKE NLGAREQVLY CYQIASGMQY 1000
LEKQRVVHRD LATRNVLVKK FNHVEITDFG LSKILKHDAD SITIKSGKVA 1050
IKWLAIEIFS KHCYTHASDV WAFGVTCWEI ITFGQSPYQG MSTDSIHNFL 1100
KDGNRLSQPP NCSQDLYQEL LRCWMADPKS RPGFEILYER FKEFCKVPQL 1150
FLENSNKISE SDLSAEERFQ TERIREMFDG NIDPQMYFDQ GSLPSMPSSP 1200
TSMATFTIPH GDLMNRMQSV NSSRYKTEPF DYGSTAQEDN SYLIPKTKEV 1250
QQSAVLYTAV TNEDGQTELS PSNGDYYNQP NTPSSSSGYY NEPHLKTKKP 1300
ETSEEAEAVQ YENEEVSQKE TCL 1323
Length:1,323
Mass (Da):150,511
Last modified:July 25, 2006 - v3
Checksum:i6B0307EE53EEFA99
GO

Sequence cautioni

The sequence BAA09729.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1179 – 11791D → H in CAA40919. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57767 mRNA. Translation: CAA40919.1.
D63426 Genomic DNA. Translation: BAA09729.1. Sequence problems.
Z70038 Genomic DNA. Translation: CAA93882.3.
PIRiE88257.
S70712.
RefSeqiNP_495962.2. NM_063561.4.
UniGeneiCel.16858.

Genome annotation databases

EnsemblMetazoaiZK1067.1a; ZK1067.1a; WBGene00002299.
GeneIDi174462.
KEGGicel:CELE_ZK1067.1.
UCSCiZK1067.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57767 mRNA. Translation: CAA40919.1 .
D63426 Genomic DNA. Translation: BAA09729.1 . Sequence problems.
Z70038 Genomic DNA. Translation: CAA93882.3 .
PIRi E88257.
S70712.
RefSeqi NP_495962.2. NM_063561.4.
UniGenei Cel.16858.

3D structure databases

ProteinModelPortali P24348.
SMRi P24348. Positions 7-644, 860-1181.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 39787. 107 interactions.
DIPi DIP-1014N.
IntActi P24348. 2 interactions.
MINTi MINT-1515688.

Proteomic databases

PaxDbi P24348.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai ZK1067.1a ; ZK1067.1a ; WBGene00002299 .
GeneIDi 174462.
KEGGi cel:CELE_ZK1067.1.
UCSCi ZK1067.1. c. elegans.

Organism-specific databases

CTDi 174462.
WormBasei ZK1067.1a ; CE03840 ; WBGene00002299 ; let-23.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000115459.
HOGENOMi HOG000016236.
KOi K04361.
PhylomeDBi P24348.

Enzyme and pathway databases

BRENDAi 2.7.10.1. 1045.
SignaLinki P24348.

Miscellaneous databases

NextBioi 884138.

Family and domain databases

Gene3Di 3.80.20.20. 2 hits.
InterProi IPR000494. EGF_rcpt_L.
IPR006211. Furin-like_Cys-rich_dom.
IPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF00757. Furin-like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF01030. Recep_L_domain. 2 hits.
[Graphical view ]
PRINTSi PR00109. TYRKINASE.
SMARTi SM00261. FU. 7 hits.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 3 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor subfamily."
    Aroian R.V., Koga M., Mendel J.E., Ohshima Y., Sternberg P.W.
    Nature 348:693-699(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic structure and 5' regulatory regions of the let-23 gene in the nematode C. elegans."
    Sakai T., Koga M., Ohshima Y.
    J. Mol. Biol. 256:548-555(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "Mutations in the Caenorhabditis elegans let-23 EGFR-like gene define elements important for cell-type specificity and function."
    Aroian R.V., Les G.M., Sternberg P.W.
    EMBO J. 13:360-366(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-368; GLY-469; CYS-700; CYS-753; THR-1065 AND GLY-1074.
  5. "Basolateral localization of the Caenorhabditis elegans epidermal growth factor receptor in epithelial cells by the PDZ protein lin-10."
    Whitfield C.W., Benard C., Barnes T., Hekimi S., Kim S.K.
    Mol. Biol. Cell 10:2087-2100(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: Bristol N2.
  6. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-376, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiLET23_CAEEL
AccessioniPrimary (citable) accession number: P24348
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi