Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24348 (LET23_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor tyrosine-protein kinase let-23

EC=2.7.10.1
Alternative name(s):
Lethal protein 23
Gene names
Name:let-23
Synonyms:kin-7
ORF Names:ZK1067.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1323 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine kinase receptor required for the induction of vulval differentiation. Possible receptor for the inductive signal required for vulval development. Activated by lin-3 and acts by way of let-60 RAS. The lin-3/let-23 pair is a simplified version of the mammalian neuregulin-ERBB network.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Apical cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein. Note: Basolateral and apical membrane of cell junctions in epithelial vulval precursor cells. Ref.5

Tissue specificity

Vulval precursor cells.

Developmental stage

Expressed during L2 and L3 larval stages.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAA09729.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Receptor
Transferase
Tyrosine-protein kinase
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processepidermal growth factor receptor signaling pathway

Inferred from sequence or structural similarity Ref.1. Source: WormBase

male genitalia development

Inferred from mutant phenotype PubMed 7607066. Source: WormBase

nematode larval development

Inferred from mutant phenotype PubMed 631558. Source: WormBase

ovulation

Inferred from mutant phenotype PubMed 9491893. Source: WormBase

positive regulation of vulval development

Inferred from mutant phenotype PubMed 3996896. Source: WormBase

   Cellular_componentapical plasma membrane

Inferred from direct assay Ref.5PubMed 8612272. Source: WormBase

basolateral plasma membrane

Inferred from direct assay PubMed 9753323. Source: WormBase

cell-cell junction

Inferred from direct assay PubMed 8612272. Source: WormBase

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lateral plasma membrane

Inferred from direct assay PubMed 8612272. Source: WormBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

epidermal growth factor-activated receptor activity

Inferred from sequence or structural similarity Ref.1. Source: WormBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 13231303Receptor tyrosine-protein kinase let-23
PRO_0000016677

Regions

Topological domain21 – 818798Extracellular Potential
Transmembrane819 – 83921Helical; Potential
Topological domain840 – 1323484Cytoplasmic Potential
Domain885 – 1152268Protein kinase
Nucleotide binding891 – 8999ATP By similarity

Sites

Active site10101Proton acceptor By similarity
Binding site9191ATP By similarity

Amino acid modifications

Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation1691N-linked (GlcNAc...) Potential
Glycosylation2551N-linked (GlcNAc...) Potential
Glycosylation3761N-linked (GlcNAc...) Ref.6
Glycosylation5611N-linked (GlcNAc...) Potential
Glycosylation6551N-linked (GlcNAc...) Potential
Glycosylation7461N-linked (GlcNAc...) Potential
Glycosylation7761N-linked (GlcNAc...) Potential
Disulfide bond220 ↔ 228 By similarity
Disulfide bond224 ↔ 236 By similarity
Disulfide bond244 ↔ 251 By similarity
Disulfide bond248 ↔ 262 By similarity
Disulfide bond263 ↔ 271 By similarity
Disulfide bond267 ↔ 279 By similarity
Disulfide bond282 ↔ 291 By similarity
Disulfide bond295 ↔ 322 By similarity
Disulfide bond326 ↔ 337 By similarity
Disulfide bond341 ↔ 356 By similarity
Disulfide bond359 ↔ 364 By similarity
Disulfide bond520 ↔ 529 By similarity
Disulfide bond524 ↔ 537 By similarity
Disulfide bond540 ↔ 549 By similarity
Disulfide bond553 ↔ 567 By similarity
Disulfide bond570 ↔ 577 By similarity
Disulfide bond574 ↔ 585 By similarity
Disulfide bond588 ↔ 604 By similarity
Disulfide bond608 ↔ 620 By similarity
Disulfide bond623 ↔ 632 By similarity
Disulfide bond627 ↔ 644 By similarity
Disulfide bond647 ↔ 660 Potential
Disulfide bond670 ↔ 693 Potential
Disulfide bond696 ↔ 703 Potential
Disulfide bond700 ↔ 715 Potential
Disulfide bond717 ↔ 731 Potential
Disulfide bond735 ↔ 750 Potential
Disulfide bond753 ↔ 763 Potential
Disulfide bond757 ↔ 771 Potential
Disulfide bond774 ↔ 787 Potential
Disulfide bond791 ↔ 805 Potential

Experimental info

Mutagenesis3681C → Y in SY10. Ref.4
Mutagenesis4691G → R in MN216. Ref.4
Mutagenesis7001C → W in MN23. Ref.4
Mutagenesis7531C → Y in SY11. Ref.4
Mutagenesis10651T → I in SY16. Ref.4
Mutagenesis10741G → E in SY7. Ref.4
Sequence conflict11791D → H in CAA40919. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24348 [UniParc].

Last modified July 25, 2006. Version 3.
Checksum: 6B0307EE53EEFA99

FASTA1,323150,511
        10         20         30         40         50         60 
MRYPPSIGSI LLIIPIFLTF FGNSNAQLWK RCVSPQDCLC SGTTNGISRY GTGNILEDLE 

        70         80         90        100        110        120 
TMYRGCRRVY GNLEITWIEA NEIKKWREST NSTVDPKNED SPLKSINFFD NLEEIRGSLI 

       130        140        150        160        170        180 
IYRANIQKIS FPRLRVIYGD EVFHDNALYI HKNDKVHEVV MRELRVIRNG SVTIQDNPKM 

       190        200        210        220        230        240 
CYIGDKIDWK ELLYDPDVQK VETTNSHQHC YQNGKSMAKC HESCNDKCWG SGDNDCQRVY 

       250        260        270        280        290        300 
RSVCPKSCSQ CFYSNSTSSY ECCDSACLGG CTGHGPKNCI ACSKYELDGI CIETCPSRKI 

       310        320        330        340        350        360 
FNHKTGRLVF NPDGRYQNGN HCVKECPPEL LIENDVCVRH CSDGHHYDAT KDVRECEKCR 

       370        380        390        400        410        420 
SSSCPKICTV DGHLTNETLK NLEGCEQIDG HLIIEHAFTY EQLKVLETVK IVSEYITIVQ 

       430        440        450        460        470        480 
QNFYDLKFLK NLQIIEGRKL HNVRWALAIY QCDDLEELSL NSLKLIKTGA VLIMKNHRLC 

       490        500        510        520        530        540 
YVSKIDWSSI ITSKGKDNKP SLAIAENRDS KLCETEQRVC DKNCNKRGCW GKEPEDCLEC 

       550        560        570        580        590        600 
KTWKSVGTCV EKCDTKGFLR NQTSMKCERC SPECETCNGL GELDCLTCRH KTLYNSDFGN 

       610        620        630        640        650        660 
RMECVHDCPV SHFPTQKNVC EKCHPTCYDN GCTGPDSNLG YGGCKQCKYA VKYENDTIFC 

       670        680        690        700        710        720 
LQSSGMNNVC VENDLPNYYI STYDTEGVIE THCEKCSISC KTCSSAGRNV VQNKCVCKHV 

       730        740        750        760        770        780 
EYQPNPSERI CMDQCPVNSF MVPDTNNTVC KKCHHECDQN YHCANGQSTG CQKCKNFTVF 

       790        800        810        820        830        840 
KGDIAQCVSE CPKNLPFSNP ANGECLDYDI ASRQRKTRMV IIGSVLFGFA VMFLFILLVY 

       850        860        870        880        890        900 
WRCQRIGKKL KIAEMVDMPE LTPIDASVRP NMSRICLIPS SELQTKLDKK LGAGAFGTVF 

       910        920        930        940        950        960 
AGIYYPKRAK NVKIPVAIKV FQTDQSQTDE MLEEATNMFR LRHDNLLKII GFCMHDDGLK 

       970        980        990       1000       1010       1020 
IVTIYRPLGN LQNFLKLHKE NLGAREQVLY CYQIASGMQY LEKQRVVHRD LATRNVLVKK 

      1030       1040       1050       1060       1070       1080 
FNHVEITDFG LSKILKHDAD SITIKSGKVA IKWLAIEIFS KHCYTHASDV WAFGVTCWEI 

      1090       1100       1110       1120       1130       1140 
ITFGQSPYQG MSTDSIHNFL KDGNRLSQPP NCSQDLYQEL LRCWMADPKS RPGFEILYER 

      1150       1160       1170       1180       1190       1200 
FKEFCKVPQL FLENSNKISE SDLSAEERFQ TERIREMFDG NIDPQMYFDQ GSLPSMPSSP 

      1210       1220       1230       1240       1250       1260 
TSMATFTIPH GDLMNRMQSV NSSRYKTEPF DYGSTAQEDN SYLIPKTKEV QQSAVLYTAV 

      1270       1280       1290       1300       1310       1320 
TNEDGQTELS PSNGDYYNQP NTPSSSSGYY NEPHLKTKKP ETSEEAEAVQ YENEEVSQKE 


TCL 

« Hide

References

« Hide 'large scale' references
[1]"The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor subfamily."
Aroian R.V., Koga M., Mendel J.E., Ohshima Y., Sternberg P.W.
Nature 348:693-699(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genomic structure and 5' regulatory regions of the let-23 gene in the nematode C. elegans."
Sakai T., Koga M., Ohshima Y.
J. Mol. Biol. 256:548-555(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bristol N2.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[4]"Mutations in the Caenorhabditis elegans let-23 EGFR-like gene define elements important for cell-type specificity and function."
Aroian R.V., Les G.M., Sternberg P.W.
EMBO J. 13:360-366(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYS-368; GLY-469; CYS-700; CYS-753; THR-1065 AND GLY-1074.
[5]"Basolateral localization of the Caenorhabditis elegans epidermal growth factor receptor in epithelial cells by the PDZ protein lin-10."
Whitfield C.W., Benard C., Barnes T., Hekimi S., Kim S.K.
Mol. Biol. Cell 10:2087-2100(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: Bristol N2.
[6]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-376, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57767 mRNA. Translation: CAA40919.1.
D63426 Genomic DNA. Translation: BAA09729.1. Sequence problems.
Z70038 Genomic DNA. Translation: CAA93882.3.
PIRE88257.
S70712.
RefSeqNP_495962.2. NM_063561.4.
UniGeneCel.16858.

3D structure databases

ProteinModelPortalP24348.
SMRP24348. Positions 7-644, 882-1152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid39787. 107 interactions.
DIPDIP-1014N.
IntActP24348. 2 interactions.
MINTMINT-1515688.

Proteomic databases

PaxDbP24348.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaZK1067.1a; ZK1067.1a; ZK1067.1.
GeneID174462.
KEGGcel:CELE_ZK1067.1.
UCSCZK1067.1. c. elegans.

Organism-specific databases

CTD174462.
WormBaseZK1067.1a; CE03840; WBGene00002299; let-23.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000016236.
KOK04361.
PhylomeDBP24348.

Enzyme and pathway databases

BRENDA2.7.10.1. 1045.
SignaLinkP24348.

Family and domain databases

Gene3D3.80.20.20. 2 hits.
InterProIPR000494. EGF_rcpt_L.
IPR006211. Furin-like_Cys-rich_dom.
IPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF00757. Furin-like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF01030. Recep_L_domain. 2 hits.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SMARTSM00261. FU. 7 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 3 hits.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio884138.

Entry information

Entry nameLET23_CAEEL
AccessionPrimary (citable) accession number: P24348
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase