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P24348

- LET23_CAEEL

UniProt

P24348 - LET23_CAEEL

Protein

Receptor tyrosine-protein kinase let-23

Gene

let-23

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 3 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Tyrosine kinase receptor required for the induction of vulval differentiation. Possible receptor for the inductive signal required for vulval development. Activated by lin-3 and acts by way of let-60 RAS. The lin-3/let-23 pair is a simplified version of the mammalian neuregulin-ERBB network.

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei919 – 9191ATPPROSITE-ProRule annotation
    Active sitei1010 – 10101Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi891 – 8999ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. epidermal growth factor-activated receptor activity Source: WormBase

    GO - Biological processi

    1. epidermal growth factor receptor signaling pathway Source: WormBase
    2. male genitalia development Source: WormBase
    3. nematode larval development Source: WormBase
    4. ovulation Source: WormBase
    5. positive regulation of vulval development Source: WormBase

    Keywords - Molecular functioni

    Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.1. 1045.
    SignaLinkiP24348.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Receptor tyrosine-protein kinase let-23 (EC:2.7.10.1)
    Alternative name(s):
    Lethal protein 23
    Gene namesi
    Name:let-23
    Synonyms:kin-7
    ORF Names:ZK1067.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome II

    Organism-specific databases

    WormBaseiZK1067.1a; CE03840; WBGene00002299; let-23.

    Subcellular locationi

    Apical cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Basolateral cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
    Note: Basolateral and apical membrane of cell junctions in epithelial vulval precursor cells.

    GO - Cellular componenti

    1. apical plasma membrane Source: WormBase
    2. basolateral plasma membrane Source: WormBase
    3. cell-cell junction Source: WormBase
    4. integral component of membrane Source: UniProtKB-KW
    5. lateral plasma membrane Source: WormBase

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi368 – 3681C → Y in SY10. 1 Publication
    Mutagenesisi469 – 4691G → R in MN216. 1 Publication
    Mutagenesisi700 – 7001C → W in MN23. 1 Publication
    Mutagenesisi753 – 7531C → Y in SY11. 1 Publication
    Mutagenesisi1065 – 10651T → I in SY16. 1 Publication
    Mutagenesisi1074 – 10741G → E in SY7. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 13231303Receptor tyrosine-protein kinase let-23PRO_0000016677Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi220 ↔ 228By similarity
    Disulfide bondi224 ↔ 236By similarity
    Disulfide bondi244 ↔ 251By similarity
    Disulfide bondi248 ↔ 262By similarity
    Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi263 ↔ 271By similarity
    Disulfide bondi267 ↔ 279By similarity
    Disulfide bondi282 ↔ 291By similarity
    Disulfide bondi295 ↔ 322By similarity
    Disulfide bondi326 ↔ 337By similarity
    Disulfide bondi341 ↔ 356By similarity
    Disulfide bondi359 ↔ 364By similarity
    Glycosylationi376 – 3761N-linked (GlcNAc...)1 Publication
    Disulfide bondi520 ↔ 529By similarity
    Disulfide bondi524 ↔ 537By similarity
    Disulfide bondi540 ↔ 549By similarity
    Disulfide bondi553 ↔ 567By similarity
    Glycosylationi561 – 5611N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi570 ↔ 577By similarity
    Disulfide bondi574 ↔ 585By similarity
    Disulfide bondi588 ↔ 604By similarity
    Disulfide bondi608 ↔ 620By similarity
    Disulfide bondi623 ↔ 632By similarity
    Disulfide bondi627 ↔ 644By similarity
    Disulfide bondi647 ↔ 660Sequence Analysis
    Glycosylationi655 – 6551N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi670 ↔ 693Sequence Analysis
    Disulfide bondi696 ↔ 703Sequence Analysis
    Disulfide bondi700 ↔ 715Sequence Analysis
    Disulfide bondi717 ↔ 731Sequence Analysis
    Disulfide bondi735 ↔ 750Sequence Analysis
    Glycosylationi746 – 7461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi753 ↔ 763Sequence Analysis
    Disulfide bondi757 ↔ 771Sequence Analysis
    Disulfide bondi774 ↔ 787Sequence Analysis
    Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi791 ↔ 805Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP24348.

    Expressioni

    Tissue specificityi

    Vulval precursor cells.

    Developmental stagei

    Expressed during L2 and L3 larval stages.

    Interactioni

    Protein-protein interaction databases

    BioGridi39787. 107 interactions.
    DIPiDIP-1014N.
    IntActiP24348. 2 interactions.
    MINTiMINT-1515688.

    Structurei

    3D structure databases

    ProteinModelPortaliP24348.
    SMRiP24348. Positions 7-644, 860-1181.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 818798ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini840 – 1323484CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei819 – 83921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini885 – 1152268Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115459.
    HOGENOMiHOG000016236.
    KOiK04361.
    PhylomeDBiP24348.

    Family and domain databases

    Gene3Di3.80.20.20. 2 hits.
    InterProiIPR000494. EGF_rcpt_L.
    IPR006211. Furin-like_Cys-rich_dom.
    IPR006212. Furin_repeat.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF00757. Furin-like. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF01030. Recep_L_domain. 2 hits.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    SMARTiSM00261. FU. 7 hits.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    SSF57184. SSF57184. 3 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24348-1 [UniParc]FASTAAdd to Basket

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    MRYPPSIGSI LLIIPIFLTF FGNSNAQLWK RCVSPQDCLC SGTTNGISRY     50
    GTGNILEDLE TMYRGCRRVY GNLEITWIEA NEIKKWREST NSTVDPKNED 100
    SPLKSINFFD NLEEIRGSLI IYRANIQKIS FPRLRVIYGD EVFHDNALYI 150
    HKNDKVHEVV MRELRVIRNG SVTIQDNPKM CYIGDKIDWK ELLYDPDVQK 200
    VETTNSHQHC YQNGKSMAKC HESCNDKCWG SGDNDCQRVY RSVCPKSCSQ 250
    CFYSNSTSSY ECCDSACLGG CTGHGPKNCI ACSKYELDGI CIETCPSRKI 300
    FNHKTGRLVF NPDGRYQNGN HCVKECPPEL LIENDVCVRH CSDGHHYDAT 350
    KDVRECEKCR SSSCPKICTV DGHLTNETLK NLEGCEQIDG HLIIEHAFTY 400
    EQLKVLETVK IVSEYITIVQ QNFYDLKFLK NLQIIEGRKL HNVRWALAIY 450
    QCDDLEELSL NSLKLIKTGA VLIMKNHRLC YVSKIDWSSI ITSKGKDNKP 500
    SLAIAENRDS KLCETEQRVC DKNCNKRGCW GKEPEDCLEC KTWKSVGTCV 550
    EKCDTKGFLR NQTSMKCERC SPECETCNGL GELDCLTCRH KTLYNSDFGN 600
    RMECVHDCPV SHFPTQKNVC EKCHPTCYDN GCTGPDSNLG YGGCKQCKYA 650
    VKYENDTIFC LQSSGMNNVC VENDLPNYYI STYDTEGVIE THCEKCSISC 700
    KTCSSAGRNV VQNKCVCKHV EYQPNPSERI CMDQCPVNSF MVPDTNNTVC 750
    KKCHHECDQN YHCANGQSTG CQKCKNFTVF KGDIAQCVSE CPKNLPFSNP 800
    ANGECLDYDI ASRQRKTRMV IIGSVLFGFA VMFLFILLVY WRCQRIGKKL 850
    KIAEMVDMPE LTPIDASVRP NMSRICLIPS SELQTKLDKK LGAGAFGTVF 900
    AGIYYPKRAK NVKIPVAIKV FQTDQSQTDE MLEEATNMFR LRHDNLLKII 950
    GFCMHDDGLK IVTIYRPLGN LQNFLKLHKE NLGAREQVLY CYQIASGMQY 1000
    LEKQRVVHRD LATRNVLVKK FNHVEITDFG LSKILKHDAD SITIKSGKVA 1050
    IKWLAIEIFS KHCYTHASDV WAFGVTCWEI ITFGQSPYQG MSTDSIHNFL 1100
    KDGNRLSQPP NCSQDLYQEL LRCWMADPKS RPGFEILYER FKEFCKVPQL 1150
    FLENSNKISE SDLSAEERFQ TERIREMFDG NIDPQMYFDQ GSLPSMPSSP 1200
    TSMATFTIPH GDLMNRMQSV NSSRYKTEPF DYGSTAQEDN SYLIPKTKEV 1250
    QQSAVLYTAV TNEDGQTELS PSNGDYYNQP NTPSSSSGYY NEPHLKTKKP 1300
    ETSEEAEAVQ YENEEVSQKE TCL 1323
    Length:1,323
    Mass (Da):150,511
    Last modified:July 25, 2006 - v3
    Checksum:i6B0307EE53EEFA99
    GO

    Sequence cautioni

    The sequence BAA09729.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1179 – 11791D → H in CAA40919. (PubMed:1979659)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57767 mRNA. Translation: CAA40919.1.
    D63426 Genomic DNA. Translation: BAA09729.1. Sequence problems.
    Z70038 Genomic DNA. Translation: CAA93882.3.
    PIRiE88257.
    S70712.
    RefSeqiNP_495962.2. NM_063561.4.
    UniGeneiCel.16858.

    Genome annotation databases

    EnsemblMetazoaiZK1067.1a; ZK1067.1a; WBGene00002299.
    GeneIDi174462.
    KEGGicel:CELE_ZK1067.1.
    UCSCiZK1067.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57767 mRNA. Translation: CAA40919.1 .
    D63426 Genomic DNA. Translation: BAA09729.1 . Sequence problems.
    Z70038 Genomic DNA. Translation: CAA93882.3 .
    PIRi E88257.
    S70712.
    RefSeqi NP_495962.2. NM_063561.4.
    UniGenei Cel.16858.

    3D structure databases

    ProteinModelPortali P24348.
    SMRi P24348. Positions 7-644, 860-1181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 39787. 107 interactions.
    DIPi DIP-1014N.
    IntActi P24348. 2 interactions.
    MINTi MINT-1515688.

    Proteomic databases

    PaxDbi P24348.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai ZK1067.1a ; ZK1067.1a ; WBGene00002299 .
    GeneIDi 174462.
    KEGGi cel:CELE_ZK1067.1.
    UCSCi ZK1067.1. c. elegans.

    Organism-specific databases

    CTDi 174462.
    WormBasei ZK1067.1a ; CE03840 ; WBGene00002299 ; let-23.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115459.
    HOGENOMi HOG000016236.
    KOi K04361.
    PhylomeDBi P24348.

    Enzyme and pathway databases

    BRENDAi 2.7.10.1. 1045.
    SignaLinki P24348.

    Miscellaneous databases

    NextBioi 884138.

    Family and domain databases

    Gene3Di 3.80.20.20. 2 hits.
    InterProi IPR000494. EGF_rcpt_L.
    IPR006211. Furin-like_Cys-rich_dom.
    IPR006212. Furin_repeat.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00757. Furin-like. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF01030. Recep_L_domain. 2 hits.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    SMARTi SM00261. FU. 7 hits.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    SSF57184. SSF57184. 3 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor subfamily."
      Aroian R.V., Koga M., Mendel J.E., Ohshima Y., Sternberg P.W.
      Nature 348:693-699(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic structure and 5' regulatory regions of the let-23 gene in the nematode C. elegans."
      Sakai T., Koga M., Ohshima Y.
      J. Mol. Biol. 256:548-555(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Bristol N2.
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    4. "Mutations in the Caenorhabditis elegans let-23 EGFR-like gene define elements important for cell-type specificity and function."
      Aroian R.V., Les G.M., Sternberg P.W.
      EMBO J. 13:360-366(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CYS-368; GLY-469; CYS-700; CYS-753; THR-1065 AND GLY-1074.
    5. "Basolateral localization of the Caenorhabditis elegans epidermal growth factor receptor in epithelial cells by the PDZ protein lin-10."
      Whitfield C.W., Benard C., Barnes T., Hekimi S., Kim S.K.
      Mol. Biol. Cell 10:2087-2100(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: Bristol N2.
    6. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-376, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiLET23_CAEEL
    AccessioniPrimary (citable) accession number: P24348
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 147 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3