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Protein

Waglerin-3

Gene
N/A
Organism
Tropidolaemus wagleri (Wagler's pit viper) (Trimeresurus wagleri)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Waglerin-1 selectively blocks the epsilon subunit of muscle nicotinic acetylcholine receptor (nAChR). Has also effects on rodent ionotropic GABA(A) receptors (GABR), since it potentiates I(GABA) in some neurons and depresses I(GABA) in others. In mice, it elicits tachypnea, ocular proptosis, rapid collapse and spasms, whereas no toxic effects on respiration and blood pressure are observed in rats.
Waglerin-3 selectively blocks the epsilon subunit of muscle nicotinic acetylcholine receptor (nAChR). It elicits tachypnea, ocular proptosis, rapid collapse and spasms in mice. It causes death by respiratory failure.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Waglerin-3
Alternative name(s):
SL-Waglerin-1
Short name:
SL-I
Cleaved into the following chain:
Alternative name(s):
Lethal peptide I
Waglerin I
Wtx-1
OrganismiTropidolaemus wagleri (Wagler's pit viper) (Trimeresurus wagleri)
Taxonomic identifieri8770 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeTropidolaemus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 of waglerin-1 is 0.33-0.369 mg/kg by intraperitoneal injection into mice.
LD50 of waglerin-3 is 0.22 mg/kg by intraperitoneal injection into mice.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi3 – 4Missing : No loss of affinity; in Waglerin-1. 1 Publication2
Mutagenesisi5K → A: No decrease in lethality; in Waglerin-1. 1 Publication1
Mutagenesisi7D → E or N: Slight decrease in lethality; in Waglerin-1. 1 Publication1
Mutagenesisi9R → A: Slight decrease in lethality; in Waglerin-1. 1 Publication1
Mutagenesisi11C → S: 3000-fold decrease in affinity at the alpha-epsilon site of nAChR and 4- to 9-fold decrease at the alpha-gamma and alpha-delta sites, respectively; when associated with S-15 in Waglerin-1. 1 Publication1
Mutagenesisi12H → A: Very important (34-fold) decrease in lethality; in Waglerin-1. 2 Publications1
Mutagenesisi12H → D: 260-fold decrease in affinity; in Waglerin-1. 2 Publications1
Mutagenesisi15C → S: 3000-fold decrease in affinity at the alpha-epsilon site of nAChR and 4- to 9-fold decrease at the alpha-gamma and alpha-delta sites, respectively; when associated with S-11 in Waglerin-1. 1 Publication1
Mutagenesisi16H → A: Important (16-fold) decrease in lethality; in Waglerin-1. 2 Publications1
Mutagenesisi16H → D: 1160-fold decrease in affinity; in Waglerin-1. 2 Publications1
Mutagenesisi20R → A: Slight decrease in lethality; in Waglerin-1. 1 Publication1
Mutagenesisi22K → A: Slight decrease in lethality; in Waglerin-1. 1 Publication1
Mutagenesisi23 – 24Missing : No loss of affinity; in Waglerin-1. 1 Publication2
Mutagenesisi24R → A: Slight decrease in lethality; in Waglerin-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00004040851 – 24Waglerin-3Add BLAST24
PeptideiPRO_00000445493 – 24Waglerin-1Add BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi11 ↔ 151 Publication

Post-translational modificationi

Amidation of the waglerin-1 C-terminus increases the affinity by 2-fold.

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Waglerin-1 is monomeric.

Family & Domainsi

Sequence similaritiesi

Belongs to the waglerin family.Curated

Family and domain databases

InterProiIPR012637. Toxin_33.
[Graphical view]
PfamiPF08121. Toxin_33. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24335-1 [UniParc]FASTAAdd to basket

« Hide

        10         20 
SLGGKPDLRP CHPPCHYIPR PKPR
Length:24
Mass (Da):2,722
Last modified:February 8, 2011 - v2
Checksum:iD9B533A32692594F
GO

Sequence databases

PIRiB44008.

Cross-referencesi

Sequence databases

PIRiB44008.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR012637. Toxin_33.
[Graphical view]
PfamiPF08121. Toxin_33. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWAG13_TROWA
AccessioniPrimary (citable) accession number: P24335
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 8, 2011
Last modified: July 22, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Exists in two forms, due to cis-trans isomerization at 13-Pro-Pro-14.

Caution

Mutagenesis results from PubMed:8858103 should be interpreted carefully, since the authors found waglerin-2 (AC P58930) as inactive, despite the finding of other groups that found this peptide equipotent in lethality to waglerin-1.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.