Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P24335

- WAG13_TROWA

UniProt

P24335 - WAG13_TROWA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Waglerin-3

Gene
N/A
Organism
Tropidolaemus wagleri (Wagler's pit viper) (Trimeresurus wagleri)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Waglerin-1 selectively blocks the epsilon subunit of muscle nicotinic acetylcholine receptor (nAChR). Has also effects on rodent ionotropic GABA(A)/GABRA1 receptors, since it potentiates I(GABA) in some neurons and depresses I(GABA) in others. In mice, it elicits tachypnea, ocular proptosis, rapid collapse and spasms, whereas no toxic effects on respiration and blood pressure are observed in rats.
Waglerin-3 selectively blocks the epsilon subunit of muscle nicotinic acetylcholine receptor (nAChR). It elicits tachypnea, ocular proptosis, rapid collapse and spasms in mice. It causes death by respiratory failure.

GO - Molecular functioni

  1. acetylcholine receptor inhibitor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Waglerin-3
Alternative name(s):
SL-Waglerin-1
Short name:
SL-I
Cleaved into the following chain:
Alternative name(s):
Lethal peptide I
Waglerin I
Wtx-1
OrganismiTropidolaemus wagleri (Wagler's pit viper) (Trimeresurus wagleri)
Taxonomic identifieri8770 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeTropidolaemus

Subcellular locationi

GO - Cellular componenti

  1. other organism postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 of waglerin-1 is 0.33-0.369 mg/kg by intraperitoneal injection into mice.
LD50 of waglerin-3 is 0.22 mg/kg by intraperitoneal injection into mice.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi3 – 42Missing: No loss of affinity; in Waglerin-1. 1 Publication
Mutagenesisi5 – 51K → A: No decrease in lethality; in Waglerin-1. 1 Publication
Mutagenesisi7 – 71D → E or N: Slight decrease in lethality; in Waglerin-1. 1 Publication
Mutagenesisi9 – 91R → A: Slight decrease in lethality; in Waglerin-1. 1 Publication
Mutagenesisi11 – 111C → S: 3000-fold decrease in affinity at the alpha-epsilon site of nAChR and 4- to 9-fold decrease at the alpha-gamma and alpha-delta sites, respectively; when associated with S-15 in Waglerin-1. 1 Publication
Mutagenesisi12 – 121H → A: Very important (34-fold) decrease in lethality; in Waglerin-1. 2 Publications
Mutagenesisi12 – 121H → D: 260-fold decrease in affinity; in Waglerin-1. 2 Publications
Mutagenesisi15 – 151C → S: 3000-fold decrease in affinity at the alpha-epsilon site of nAChR and 4- to 9-fold decrease at the alpha-gamma and alpha-delta sites, respectively; when associated with S-11 in Waglerin-1. 1 Publication
Mutagenesisi16 – 161H → A: Important (16-fold) decrease in lethality; in Waglerin-1. 2 Publications
Mutagenesisi16 – 161H → D: 1160-fold decrease in affinity; in Waglerin-1. 2 Publications
Mutagenesisi20 – 201R → A: Slight decrease in lethality; in Waglerin-1. 1 Publication
Mutagenesisi22 – 221K → A: Slight decrease in lethality; in Waglerin-1. 1 Publication
Mutagenesisi23 – 242Missing: No loss of affinity; in Waglerin-1. 1 Publication
Mutagenesisi24 – 241R → A: Slight decrease in lethality; in Waglerin-1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 2424Waglerin-3PRO_0000404085Add
BLAST
Peptidei3 – 2422Waglerin-1PRO_0000044549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi11 ↔ 151 Publication

Post-translational modificationi

Amidation of the waglerin-1 C-terminus increases the affinity by 2-fold.

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Waglerin-1 is monomeric.

Family & Domainsi

Sequence similaritiesi

Belongs to the waglerin family.Curated

Family and domain databases

InterProiIPR012637. Toxin_33.
[Graphical view]
PfamiPF08121. Toxin_33. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24335-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20
SLGGKPDLRP CHPPCHYIPR PKPR
Length:24
Mass (Da):2,722
Last modified:February 8, 2011 - v2
Checksum:iD9B533A32692594F
GO

Sequence databases

PIRiB44008.

Cross-referencesi

Sequence databases

PIRi B44008.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR012637. Toxin_33.
[Graphical view ]
Pfami PF08121. Toxin_33. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular properties and structure-function relationships of lethal peptides from venom of Wagler's pit viper, Trimeresurus wagleri."
    Schmidt J.J., Weinstein S.A., Smith L.A.
    Toxicon 30:1027-1036(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE (WAGLERIN-3), SYNTHESIS OF 1-24 (WAGLERIN-3) AND 3-24 (WAGLERIN-1), DISULFIDE BOND, LETHAL DOSE.
    Tissue: Venom.
  2. "Characterization and amino acid sequences of two lethal peptides isolated from venom of Wagler's pit viper, Trimeresurus wagleri."
    Weinstein S.A., Schmidt J.J., Bernheimer A.W., Smith L.A.
    Toxicon 29:227-236(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-24 (WAGLERIN-1), LETHAL DOSE.
    Tissue: Venom.
  3. "A study on the cause of death due to waglerin-I, a toxin from Trimeresurus wagleri."
    Lin W.W., Smith L.A., Lee C.Y.
    Toxicon 33:111-114(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION (WAGLERIN-1).
  4. "Structure-function studies of waglerin I, a lethal peptide from the venom of Wagler's pit viper, Trimeresurus wagleri."
    Schmidt J.J., Weinstein S.A.
    Toxicon 33:1043-1049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-7.
  5. "Protein engineering of venom toxins by synthetic approach and NMR dynamic simulation: status of basic amino acid residues in waglerin I."
    Hsiao Y.-M., Chuang C.-C., Chuang L.-C., Yu H.-M., Wang K.-T., Chiou S.-H., Wu S.-H.
    Biochem. Biophys. Res. Commun. 227:59-63(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: LETHAL DOSE, SYNTHESIS, MUTAGENESIS OF LYS-5; ARG-9; HIS-12; HIS-16; ARG-20; LYS-22 AND ARG-24.
  6. "Waglerin-1 modulates gamma-aminobutyric acid activated current of murine hypothalamic neurons."
    Ye J.-H., McArdle J.J.
    J. Pharmacol. Exp. Ther. 282:74-80(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOXIN TARGET.
    Tissue: Venom.
  7. "Waglerin-1 selectively blocks the epsilon form of the muscle nicotinic acetylcholine receptor."
    McArdle J.J., Lentz T.L., Witzemann V., Schwarz H., Weinstein S.A., Schmidt J.J.
    J. Pharmacol. Exp. Ther. 289:543-550(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION (WAGLERIN-1), TOXIN TARGET.
  8. "Identification of residues at the alpha and epsilon subunit interfaces mediating species selectivity of Waglerin-1 for nicotinic acetylcholine receptors."
    Molles B.E., Rezai P., Kline E.F., McArdle J.J., Sine S.M., Taylor P.
    J. Biol. Chem. 277:5433-5440(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOXIN TARGET.
  9. "Structure and function of the waglerins, peptide toxins from the venom of Wagler's Pit Viper, Tropidolaemus wagleri."
    Molles B.E., Taylor P.
    J. Toxicol. Toxin Rev. 21:273-292(2002)
    Cited for: FUNCTION, TOXIN TARGET, MUTAGENESIS OF 3-GLY-GLY-4; 23-PRO-ARG-24; CYS-11; HIS-12; CYS-15 AND HIS-16, REVIEW.
  10. "Determination of three-dimensional solution structure of waglerin I, a toxin from Trimeresurus wagleri, using 2D-NMR and molecular dynamics simulation."
    Chuang L.C., Yu H.M., Chen C., Huang T.H., Wu S.H., Wang K.T.
    Biochim. Biophys. Acta 1292:145-155(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 3-24, SYNTHESIS OF 3-24, ISOMERIZATION (WAGLERIN-1).
  11. "Conformational analysis of a toxic peptide from Trimeresurus wagleri which blocks the nicotinic acetylcholine receptor."
    Sellin L.C., Mattila K., Annila A., Schmidt J.J., McArdle J.J., Hyvonen M., Rantala T.T., Kivisto T.
    Biophys. J. 70:3-13(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 3-24.

Entry informationi

Entry nameiWAG13_TROWA
AccessioniPrimary (citable) accession number: P24335
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 8, 2011
Last modified: October 29, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Exists in two forms, due to cis-trans isomerization at 13-Pro-Pro-14.

Caution

Mutagenesis results from PubMed:8858103 should be interpreted carefully, since the authors found waglerin-2 (AC P58930) as inactive, despite the finding of other groups that found this peptide equipotent in lethality to waglerin-1.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3