ID   MYX_CROAD               Reviewed;          70 AA.
AC   P24330; F8S0Z6; J3RY57;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2013, sequence version 2.
DT   22-FEB-2023, entry version 81.
DE   RecName: Full=Myotoxin;
DE   AltName: Full=CAM-toxin;
DE   Flags: Precursor;
OS   Crotalus adamanteus (Eastern diamondback rattlesnake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Crotalus.
OX   NCBI_TaxID=8729;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=23025625; DOI=10.1186/1471-2164-13-312;
RA   Rokyta D.R., Lemmon A.R., Margres M.J., Aronow K.;
RT   "The venom-gland transcriptome of the eastern diamondback rattlesnake
RT   (Crotalus adamanteus).";
RL   BMC Genomics 13:312-312(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-63.
RX   PubMed=21255598; DOI=10.1016/j.toxicon.2011.01.008;
RA   Rokyta D.R., Wray K.P., Lemmon A.R., Lemmon E.M., Caudle S.B.;
RT   "A high-throughput venom-gland transcriptome for the eastern diamondback
RT   rattlesnake (Crotalus adamanteus) and evidence for pervasive positive
RT   selection across toxin classes.";
RL   Toxicon 57:657-671(2011).
RN   [3]
RP   PROTEIN SEQUENCE OF 23-67, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=1862521; DOI=10.1016/0041-0101(91)90020-r;
RA   Samejima Y., Aoki Y., Mebs D.;
RT   "Amino acid sequence of a myotoxin from venom of the eastern diamondback
RT   rattlesnake (Crotalus adamanteus).";
RL   Toxicon 29:461-468(1991).
RN   [4]
RP   PROTEIN SEQUENCE OF 23-34, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=24231107; DOI=10.1016/j.jprot.2013.11.001;
RA   Margres M.J., McGivern J.J., Wray K.P., Seavy M., Calvin K., Rokyta D.R.;
RT   "Linking the transcriptome and proteome to characterize the venom of the
RT   eastern diamondback rattlesnake (Crotalus adamanteus).";
RL   J. Proteomics 96:145-158(2014).
CC   -!- FUNCTION: Cationic peptide that possesses multiple functions. It acts
CC       as a cell-penetrating peptide (CPP), and as a potent voltage-gated
CC       potassium channel (Kv) inhibitor. It exhibits antimicrobial activities,
CC       hind limb paralysis, and severe muscle necrosis by a non-enzymatic
CC       mechanism. {ECO:0000250|UniProtKB:Q9PWF3}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1862521}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:1862521}.
CC   -!- TOXIC DOSE: LD(50) is 0.96 mg/kg by subcutaneous injection.
CC       {ECO:0000269|PubMed:1862521}.
CC   -!- MISCELLANEOUS: This myotoxin is the most abundant transcript in the
CC       venom of the specimen analyzed in PubMed:23025625.
CC   -!- MISCELLANEOUS: Individuals of C.adamanteus from populations in southern
CC       and central Florida lack this toxin in their venoms.
CC       {ECO:0000305|PubMed:23025625}.
CC   -!- SIMILARITY: Belongs to the crotamine-myotoxin family. {ECO:0000305}.
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DR   EMBL; HQ414100; AEJ31978.1; -; mRNA.
DR   EMBL; JU173668; AFJ49194.1; -; mRNA.
DR   PIR; A37909; A37909.
DR   AlphaFoldDB; P24330; -.
DR   SMR; P24330; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044564; P:envenomation resulting in occlusion of the pore of voltage-gated potassium channel in another organism; ISS:UniProtKB.
DR   Gene3D; 2.20.20.10; Anthopleurin-A; 1.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR   InterPro; IPR000881; Myotoxin.
DR   Pfam; PF00819; Myotoxins; 1.
DR   PRINTS; PR00283; MYOTOXIN.
DR   SUPFAM; SSF57392; Defensin-like; 1.
DR   PROSITE; PS00459; MYOTOXINS_1; 1.
DR   PROSITE; PS51345; MYOTOXINS_2; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Myotoxin; Neurotoxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:1862521,
FT                   ECO:0000269|PubMed:24231107"
FT   CHAIN           23..70
FT                   /note="Myotoxin"
FT                   /evidence="ECO:0000305|PubMed:1862521"
FT                   /id="PRO_0000221562"
FT   DISULFID        26..58
FT                   /evidence="ECO:0000250|UniProtKB:Q9PWF3"
FT   DISULFID        33..52
FT                   /evidence="ECO:0000250|UniProtKB:Q9PWF3"
FT   DISULFID        40..59
FT                   /evidence="ECO:0000250|UniProtKB:Q9PWF3"
FT   CONFLICT        63
FT                   /note="S -> V (in Ref. 2; AEJ31978)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   70 AA;  8017 MW;  21432AE06B0989EE CRC64;
     MKILYLLFAF LFLAFLSEPG NAYKRCHKKG GHCFPKTVIC LPPSSDFGKM DCRWRWKCCK
     KGSVNNAISI
//