P24330 (MYX_CROAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myotoxin Alternative name(s): CAM-toxin
Organism	Crotalus adamanteus (Eastern diamondback rattlesnake)
Taxonomic identifier	8729 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Crotalus

Protein attributes

Sequence length	45 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This toxin specifically modifies voltage-gated sodium channels (Nav), and it exhibits analgesic activity. Moreover, it actively interacts with lipid membranes By similarity. It causes severe muscle necrosis by a non-enzymatic mechanism.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Toxic dose	LD50 is 0.96 mg/kg by subcutaneous injection. Ref.1
Sequence similarities	Belongs to the snake myotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Myotoxin Sodium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 45	45	Myotoxin		PRO_0000221562
Amino acid modifications
	Disulfide bond	4 ↔ 36		By similarity
	Disulfide bond	11 ↔ 30		By similarity
	Disulfide bond	18 ↔ 37		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24330 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 8A12DD3B42A5DF3A	FASTA	45	5,202
	10 20 30 40 YKRCHKKGGH CFPKTVICLP PSSDFGKMDC RWRWKCCKKG SVNNA

References

[1]	"Amino acid sequence of a myotoxin from venom of the eastern diamondback rattlesnake (Crotalus adamanteus)." Samejima Y., Aoki Y., Mebs D. Toxicon 29:461-468(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, LETHAL DOSE. Tissue: Venom.

Cross-references

Sequence databases
PIR	A37909.
3D structure databases
ProteinModelPortal	P24330.
SMR	P24330. Positions 1-41.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG006434.
Family and domain databases
Gene3D	2.20.20.10. 1 hit.
InterPro	IPR023355. Myo_neuro_toxin. IPR000881. Myotoxin. [Graphical view]
Pfam	PF00819. Myotoxins. 1 hit. [Graphical view]
PRINTS	PR00283. MYOTOXIN.
ProDom	PD005972. Myotoxin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00459. MYOTOXINS_1. 1 hit. PS51345. MYOTOXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MYX_CROAD

Accession

Primary (citable) accession number: P24330

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program

Annotation program

Chordata Protein Annotation Program