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Protein

Myotoxin

Gene
N/A
Organism
Crotalus adamanteus (Eastern diamondback rattlesnake)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cationic peptide that possess multiple functions. It acts as a cell-penetrating peptide (CPP), and as a potent voltage-gated potassium channel (Kv) inhibitor. It exhibits antimicrobial activities, hind limb paralysis, and severe muscle necrosis by a non-enzymatic mechanism (By similarity).By similarity

GO - Biological processi

  1. envenomation resulting in occlusion of the pore of voltage-gated potassium channel in other organism Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Ion channel impairing toxin, Myotoxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Myotoxin
Alternative name(s):
CAM-toxin
OrganismiCrotalus adamanteus (Eastern diamondback rattlesnake)
Taxonomic identifieri8729 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeCrotalus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.96 mg/kg by subcutaneous injection.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22222 PublicationsAdd
BLAST
Chaini23 – 7048MyotoxinPRO_0000221562Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 58PROSITE-ProRule annotation
Disulfide bondi33 ↔ 52PROSITE-ProRule annotation
Disulfide bondi40 ↔ 59PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Family & Domainsi

Sequence similaritiesi

Belongs to the crotamine-myotoxin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006434.

Family and domain databases

Gene3Di2.20.20.10. 1 hit.
InterProiIPR023355. Myo_neuro_toxin.
IPR000881. Rattlesnake_Myotoxin.
[Graphical view]
PfamiPF00819. Myotoxins. 1 hit.
[Graphical view]
PRINTSiPR00283. MYOTOXIN.
ProDomiPD005972. Myotoxin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00459. MYOTOXINS_1. 1 hit.
PS51345. MYOTOXINS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILYLLFAF LFLAFLSEPG NAYKRCHKKG GHCFPKTVIC LPPSSDFGKM
60 70
DCRWRWKCCK KGSVNNAISI
Length:70
Mass (Da):8,017
Last modified:November 12, 2013 - v2
Checksum:i21432AE06B0989EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631S → V in AEJ31978 (PubMed:21255598).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ414100 mRNA. Translation: AEJ31978.1.
JU173668 mRNA. Translation: AFJ49194.1.
PIRiA37909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ414100 mRNA. Translation: AEJ31978.1.
JU173668 mRNA. Translation: AFJ49194.1.
PIRiA37909.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006434.

Family and domain databases

Gene3Di2.20.20.10. 1 hit.
InterProiIPR023355. Myo_neuro_toxin.
IPR000881. Rattlesnake_Myotoxin.
[Graphical view]
PfamiPF00819. Myotoxins. 1 hit.
[Graphical view]
PRINTSiPR00283. MYOTOXIN.
ProDomiPD005972. Myotoxin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00459. MYOTOXINS_1. 1 hit.
PS51345. MYOTOXINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)."
    Rokyta D.R., Lemmon A.R., Margres M.J., Aronow K.
    BMC Genomics 13:312-312(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "A high-throughput venom-gland transcriptome for the eastern diamondback rattlesnake (Crotalus adamanteus) and evidence for pervasive positive selection across toxin classes."
    Rokyta D.R., Wray K.P., Lemmon A.R., Lemmon E.M., Caudle S.B.
    Toxicon 57:657-671(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-63.
  3. "Amino acid sequence of a myotoxin from venom of the eastern diamondback rattlesnake (Crotalus adamanteus)."
    Samejima Y., Aoki Y., Mebs D.
    Toxicon 29:461-468(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-67, LETHAL DOSE.
    Tissue: Venom.
  4. "Linking the transcriptome and proteome to characterize the venom of the eastern diamondback rattlesnake (Crotalus adamanteus)."
    Margres M.J., McGivern J.J., Wray K.P., Seavy M., Calvin K., Rokyta D.R.
    J. Proteomics 96:145-158(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-34, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Venom.

Entry informationi

Entry nameiMYX_CROAD
AccessioniPrimary (citable) accession number: P24330
Secondary accession number(s): F8S0Z6, J3RY57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 1992
Last sequence update: November 12, 2013
Last modified: January 6, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

This myotoxin is the most abundant transcript in the venom of the specimen analyzed in PubMed:23025625.
Individuals of C.adamanteus from populations in southern and central Florida lack this toxin in their venoms.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.