P24328 (PERT_BORPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pertactin autotransporter

Alternative name(s):
P.95

Cleaved into the following 2 chains:

1. Outer membrane protein P.70
2. Pertactin translocator

Gene names

Name:	prn
Ordered Locus Names:	BPP1150

Organism

Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) [Complete proteome] [HAMAP]

Taxonomic identifier

257311 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella › Bordetella parapertussis

Protein attributes

Sequence length	922 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Agglutinogen that binds to eukaryotic cells; a process mediated by the R-G-D sequence. Pertactin may have a role in bacterial adhesion, and thus play a role in virulence. May contribute to the disease state of whooping cough.
Subunit structure	Monomer.
Subcellular location	Pertactin autotransporter: Periplasm By similarity. Outer membrane protein P.70: Secreted. Cell surface. Pertactin translocator: Cell outer membrane; Multi-pass membrane protein Potential. Note: The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane.
Domain	The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage By similarity.
Miscellaneous	Synthesized only in the presence of low Mg2+ concentrations.
Sequence similarities	Contains 1 autotransporter (TC 1.B.12) domain. [View classification]

Ontologies

Keywords
Biological process	Cell adhesion Virulence
Cellular component	Cell membrane Cell outer membrane Membrane Periplasm Secreted
Domain	Repeat Signal Transmembrane Transmembrane beta strand
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 922	888	Pertactin autotransporter		PRO_0000002708
	Chain	35 – 643	609	Outer membrane protein P.70		PRO_0000002709
	Chain	644 – 922	279	Pertactin translocator		PRO_0000002710
Regions
	Repeat	266 – 270	5	1
	Repeat	271 – 275	5	2
	Repeat	276 – 280	5	3
	Repeat	281 – 285	5	4; approximate
	Domain	654 – 922	269	Autotransporter
	Region	266 – 290	25	4 X 5 AA tandem repeats of G-G-A-V-P
	Region	575 – 603	29	9 X 3 AA approximate repeats of P-Q-P
	Motif	260 – 262	3	Cell attachment site; involved in adhesion to various eukaryotic cell lines
Sites
	Site	643 – 644	2	Cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24328 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 3DF7BC58D4712478	FASTA	922	95,178
	10 20 30 40 50 60 MNMSLSRIVK AAPLRRTTLA MALGALGAAP AAYADWNNQS IIKAGERQHG IHIKQSDGAG 70 80 90 100 110 120 VRTATGTTIK VSGRQAQGVL LENPAAELRF QNGSVTSSGQ LFDEGVRRFL GTVTVKAGKL 130 140 150 160 170 180 VADHATLANV SDTRDDDGIA LYVAGEQAQA SIADSTLQGA GGVRVERGAN VTVQRSTIVD 190 200 210 220 230 240 GGLHIGTLQP LQPEDLPPSR VVLGDTSVTA VPASGAPAAV FVFGANELTV DGGHITGGRA 250 260 270 280 290 300 AGVAAMDGAI VHLQRATIRR GDAPAGGAVP GGAVPGGAVP GGFGPLLDGW YGVDVSDSTV 310 320 330 340 350 360 DLAQSIVEAP QLGAAIRAGR GARVTVSGGS LSAPHGNVIE TGGGARRFPP PASPLSITLQ 370 380 390 400 410 420 AGARAQGRAL LYRVLPEPVK LTLAGGAQGQ GDIVATELPP IPGASSGPLD VALASQARWT 430 440 450 460 470 480 GATRAVDSLS IDNATWVMTD NSNVGALRLA SDGSVDFQQP AEAGRFKVLM VDTLAGSGLF 490 500 510 520 530 540 RMNVFADLGL SDKLVVMRDA SGQHRLWVRN SGSEPASGNT MLLVQTPRGS AATFTLANKD 550 560 570 580 590 600 GKVDIGTYRY RLAANGNGQW SLVGAKAPPA PKPAPQPGPQ PGPQPPQPPQ PPQPPQPPQP 610 620 630 640 650 660 PQRQPEAPAP QPPAGRELSA AANAAVNTGG VGLASTLWYA ESNALSKRLG ELRLNPDAGG 670 680 690 700 710 720 AWGRGFAQRQ QLDNRAGRRF DQKVAGFELG ADHAVAVAGG RWHLGGLAGY TRGDRGFTGD 730 740 750 760 770 780 GGGHTDSVHV GGYATYIANS GFYLDATLRA SRLENDFKVA GSDGYAVKGK YRTHGVGVSL 790 800 810 820 830 840 EAGRRFAHAD GWFLEPQAEL AVFRVGGGAY RAANGLRVRD EGGSSVLGRL GLEVGKRIEL 850 860 870 880 890 900 AGGRQVQPYI KASVLQEFDG AGTVRTNGIA HRTELRGTRA ELGLGMAAAL GRGHSLYASY 910 920 EYSKGPKLAM PWTFHAGYRY SW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"P.70 pertactin, an outer-membrane protein from Bordetella parapertussis: cloning, nucleotide sequence and surface expression in Escherichia coli." Li L.J., Dougan G., Novotny P., Charles I.G. Mol. Microbiol. 5:409-417(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CN2591.
[2]	"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54547 Genomic DNA. Translation: CAA38419.1. BX640426 Genomic DNA. Translation: CAE36451.1.
PIR	S15204.
RefSeq	NP_883467.1. NC_002928.3.
3D structure databases
ProteinModelPortal	P24328.
SMR	P24328. Positions 35-569.
ModBase	Search...
Protein-protein interaction databases
STRING	257311.BPP1150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE36451; CAE36451; BPP1150.
GeneID	1665367.
KEGG	bpa:BPP1150.
PATRIC	21145948. VBIBorPar43418_1186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3468.
HOGENOM	HOG000115499.
KO	K12681.
OMA	YATYIAN.
ProtClustDB	CLSK494418.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP1150-MONOMER.
Family and domain databases
Gene3D	2.160.20.20. 1 hit.
InterPro	IPR005546. Auto_transptbeta. IPR006315. OM_autotransptr_brl. IPR012332. P22_tailspike_C-like. IPR011050. Pectin_lyase_fold/virulence. IPR003992. Pertactin. IPR004899. Pertactin_central. IPR003991. Pertactin_virulence_factor. [Graphical view]
Pfam	PF03797. Autotransporter. 1 hit. PF03212. Pertactin. 1 hit. [Graphical view]
PRINTS	PR01482. PERTACTIN. PR01484. PRTACTNFAMLY.
SMART	SM00869. Autotransporter. 1 hit. [Graphical view]
SUPFAM	SSF103515. Auto_transptbeta. 1 hit. SSF51126. Pectin_lyas_like. 1 hit.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PERT_BORPA

Accession

Primary (citable) accession number: P24328

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program